scholarly journals STUDI PERTUMBUHAN DAN DEGRADASI FENOL OLEH KULTUR TUNGGAL AKTINOMISETES DARI TANAH GAMBUT

2020 ◽  
Vol 13 (1) ◽  
pp. 95-104
Author(s):  
Tiara Elsita Masni ◽  
Tetty Marta Linda ◽  
Bernadeta Leni Fibriarti
Keyword(s):  
Water Quality ◽  
Total Population ◽  
Peat Soil ◽  
Growth Potential ◽  
Phenol Concentration ◽  
Minimal Salt Medium ◽  
Salt Medium ◽  
Quality Degradation ◽  
Initial Phenol Concentration ◽  
Degradation Ability

AbstrakFenol adalah senyawa organik yang bersifat toksik dan larut dalam air, sehingga mudah menimbulkan pencemaran pada perairan dan menurunkan kualitas air. Penelitian ini bertujuan untuk melihat potensi tiga isolat aktinomisetes asal tanah gambut Riau dalam Minimal Salt Medium yang mengandung fenol pada konsentrasi 0 ppm, 200 ppm, 400 ppm, dan 600 ppm serta mengetahui kemampuan aktinomisetes dalam mendegradasi fenol pada konsentrasi 600 ppm menggunakan metode folin ciocalteau. Potensi pertumbuhan isolat L121, L18, L11 menunjukkan total populasi tidak berbeda nyata dengan penambahan 400 ppm dan 600 ppm fenol, tetapi berbeda nyata terhadap 0 ppm dan 200 ppm fenol. Potensi pertumbuhan tertinggi terdapat pada isolat L121 dan terendah pada isolat L11. Kemampuan degradasi  fenol oleh  masing-masing isolat adalah  L121 sebesar 570,80 ppm (95%),  L18 sebesar 218,85 ppm (36%)  dan L11 sebesar  97,21 ppm (16%)  dari konsentrasi fenol awal 600 ppm pada Minimal Salt Medium. Isolat aktinomisetes ini berpotensi dikembangkan untuk penanggulangan pencemaran di lingkungan.Abstract Phenol is an organic compound is toxic and easily soluble in water so easy to cause pollution in a waters such as water quality degradation. The aim of this research is to see the potential of three isolates of actinomycetes from Riau peat soil in Minimal Salt Medium containing phenol concentration 0 ppm, 200 ppm, 400 ppm and 600 ppm and to know the ability of actinomycetes in degradation of phenol at the concentration of 600 ppm using folin ciocalteu. The growth potential of L121, L18, L11 isolates showed the total population was not significantly different with the addition of 400 ppm and 600 ppm of phenol but significantly different from 0 ppm and 200 ppm of phenol. The highest growth potential was found in L121 isolate and lowest in L11 isolate. The degradation ability of phenols by each isolate was L121 570.80 ppm (95%), L18 218.85 ppm (36%) and L11 was able to degrade phenol 97.21 ppm (16%) from the initial phenol concentration of 600 ppm at Minimum Salt Medium.These actinomycetes have the potential to be developed for the overcome of pollution in the environment.

scholarly journals Wild type Bacillus subtilis treated with ethyl methyl sulphonate produced catabolite insensitive mutants with improved cellulase production

2021 ◽  
Author(s):  
Oladipo Olaniyi
Keyword(s):  
Bacillus Subtilis ◽  
Carbon Source ◽  
Enzyme Production ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Minimal Salt Medium ◽  
Salt Medium ◽  
Production Medium ◽  
Wild Type ◽  
Ethyl Methyl

Abstract The goal of this present investigation was to mutagenize Bacillus subtilis with Ethyl Methyl Sulphonate (EMS), screen the mutants for cellulase production and evaluate the influence of different glucose concentrations on their cellulase production potentials. The wild type B. subtilis was treated with 20, 40, 60 and 80 µl of EMS and the mutants generated were screened for cellulase production in minimal salt medium containing carboxylmethylcellulose (CMC) as the carbon source. Quantitatively, cellulase activity and protein contents were determined by dinitrosalicylic acid and Lowry methods respectively. Seven mutants were developed from each of the EMS concentration bringing the total to twenty-eight from all the concentrations. Approximately 14 and 57% of the mutants developed from 40 and 60µl of EMS had higher cellulase activities than the wild type, while none of the mutants developed from 20 and 80 µl of EMS had better activities than the wild type. The supplementation of 0.2, 0.5, 1.0 and 1.5% glucose in enzyme production medium caused approximately 100, 14, 29 and 14% cellulase repression respectively in the mutants developed from 60µl EMS. Mutants MSSS02 and MSSS05 were considered as catabolite insensitive mutants because their cellulase production were enhanced in comparison to wild type.

scholarly journals Evidence for Enhanced Dissipation of Chlorpyrifos in an Agricultural Soil Inoculated with Serratia Rubidaea Strain ABS 10

2021 ◽  
Author(s):  
Asma Ben Salem ◽  
Hanene Chaabane ◽  
Tesnime Ghazouani ◽  
Pierluigi Caboni ◽  
Valentina Coroneo ◽  
...  
Keyword(s):  
16S Rrna ◽  
Bacterial Strain ◽  
Agricultural Soil ◽  
Liquid Culture ◽  
Minimal Salt Medium ◽  
Salt Medium ◽  
Sterile Soil ◽  
Rrna Sequencing ◽  
Biochemical Analyses ◽  
Serratia Rubidaea

Abstract Important mineralization of 14C-chlorpyrifos was found in a Tunisian soil exposed repeatedly to this insecticide. A bacterial strain able to grow in minimal salt medium (MSM) supplemented with 25 mg L− 1 of chlorpyrifos was isolated from this soil. It was characterized as Serratia rubidaea strain ABS 10 using morphological and biochemical analyses, as well as 16S rRNA sequencing. In liquid culture S. rubidaea stain ABS 10 was able to almost entirely dissipate chlorpyrifos within 48 hours of incubation. Although, S. rubidaea strain ABS 10 was able to grow on MSM supplemented with chlorpyrifos and to dissipate it in liquid culture, it was not able to mineralize 14C-chlorpyrifos. Therefore, one can conclude that the dissipation capability of this bacteria might be attributed to its capacity to adsorb CHL. In both non-sterile and sterile soil inoculated with S. rubidaea strain ABS 10, chlorpyrifos was more rapidly dissipated than in respective controls.

Decision Making Under Deep Uncertainty With Fuzzy Algorithm in Framework of Multi-Model Approach

2018 ◽  
pp. 1-34
Author(s):  
Yuriy V. Kostyuchenko ◽  
Yulia Stoyka ◽  
Iurii Negoda ◽  
Ivan Kopachevsky
Keyword(s):  
Water Quality ◽  
Risk Management ◽  
Decision Support ◽  
Soft Computing ◽  
Deep Uncertainty ◽  
Fuzzy Algorithm ◽  
Quality Degradation ◽  
Spatially Distributed ◽  
Distributed Measurement ◽  
Model Approach

Task of soft computing for decision support in field of risk management is analyzed in this chapter. Multi-model approach is described. Interrelations between models, remote sensing data and forecasting are described. Method of water quality assessment using satellite observation is described. Method is based on analysis of spectral reflectance of aquifers. Correlations between reflectance and pollutions are quantified. Fuzzy logic based approach for decision support in field of water quality degradation risk is discussed. Decision on water quality is making based on fuzzy algorithm using limited set of uncertain parameters. It is shown that this algorithm allows estimate water quality degradation rate and pollution risks. Using proposed approach, maps of surface water pollution risk from point and diffuse sources are calculated. Conclusions concerned soft computing in risk management are proposed and discussed. It was demonstrated, that basing on spatially distributed measurement data, proposed approach allows to calculate risk parameters with resolution close to observations.

Variabilities in the cost equivalency of decentralized treatment units designed to address network-derived water quality degradation

2006 ◽  
Vol 6 (4) ◽  
pp. 45-56
Author(s):  
J.W. Norton ◽  
W.J. Weber
Keyword(s):  
Water Quality ◽  
Water Distribution ◽  
Distribution Network ◽  
Treatment Plant ◽  
Water Distribution Network ◽  
Functional Requirements ◽  
Treatment Unit ◽  
Advantages And Disadvantages ◽  
Quality Degradation ◽  
The Cost

Factors impacting the cost equivalency point for deploying strategically-located treatment units within a distributed optimal technology network (DOT-Net) to manage network-derived water quality degradation are defined and quantified. The cost equivalency point is essentially the ‘breakeven’ allowable cost for implementing DOT-Net strategy as an alternative to upgrading a central treatment facility to ‘pretreat’ water sufficiently to manage quality degradation within a potable water distribution network. For the purposes of the analysis presented, water quality is assumed to degrade linearly with time as it flows through the distribution network. Disinfection by-product (DBP) formation, selected as a representative water quality degradation parameter, was modeled to predict service population DBP exposure and resulting cost of centralized treatment plant upgrades to meet water quality goals. The equivalency point was determined by apportioning the anticipated cost for upgrading the centralized treatment facilities over the fraction of service connections receiving deficient quality water. Both concentration of DBP precursor material and service population size are found to have limited impact on the equivalency point of a distributed treatment unit. The advantages and disadvantages of various treatment methods available for in-network water treatment are outlined and the ancillary functional requirements of the distributed treatment unit are delineated.

scholarly journals Water Quality of the Mun River in Thailand—Spatiotemporal Variations and Potential Causes

2019 ◽  
Vol 16 (20) ◽  
pp. 3906 ◽  
Author(s):  
Haoyu Tian ◽  
Guo-An Yu ◽  
Ling Tong ◽  
Renzhi Li ◽  
He Qing Huang ◽  
...  
Keyword(s):  
Water Quality ◽  
Agricultural Land ◽  
Kendall Test ◽  
Coliform Bacteria ◽  
Monitoring Site ◽  
Spatiotemporal Variations ◽  
Primary Parameter ◽  
Quality Degradation ◽  
Spatial Fluctuations

The water quality of the Mun River, one of the largest tributaries of the Mekong River and an important agricultural area in Thailand, is investigated to determine its status, identify spatiotemporal variations and distinguish the potential causes. Water quality dataset based on monitoring in the last two decades (1997–2017) from 21 monitoring sites distributed across the basin were analyzed using seasonal Kendall test and water quality index (WQI) method. The Kendall test shows significant declines in fecal coliform bacteria (FCB) and ammonia (NH3) in the upper reaches and increases in nitrate (NO3) and NH3 in the lower reaches. Strong temporal and spatial fluctuations were observed in both the concentrations of individual parameters and the WQI values. Seasonal variation of water quality was observed at each monitoring site. WQI values in August (flood season) were generally among the lowest, compared to other seasons. Spatially, sites in the upper reaches generally having lower WQI values than those in the lower reaches. Excessive phosphorus is the primary cause of water quality degradation in the upper reaches, while nitrogen is the primary parameter for water quality degradation in the lower reaches. Urban built-up land is an important “source” of water pollutants in the lower basin, while agricultural land plays a dual role, affecting across the basin.

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