scholarly journals Struktur Anatomi Daun Phyllanthaceae di Kabupaten Banggai Kepulauan

2021 ◽  
Vol 14 (1) ◽  
pp. 29-41
Author(s):  
Tri Yuni Indah Wulansari ◽  
Asih Perwita Dewi

Abstrak Pengenalan ciri makhluk hidup dalam praktik identifikasi sebagian besar menggunakan ciri morfologi. Ciri anatomi memperkuat ciri morfologi atau menyelesaikan permasalahan kerancuan identifikasi secara morfologi. Penelitian ini bertujuan untuk mengetahui karakter anatomi daun pada 11 spesies famili Phyllanthaceae yang ditemukan di wilayah eksplorasi Kabupaten Banggai Kepulauan. Metode yang digunakan adalah pembuatan preparat paradermal dan transversal helai dan tangkai daun. Karakter yang diamati pada setiap preparat adalah karakter paradermal yaitu epidermis dan derivatnya, karakter transversal meliputi bentuk dan jumlah lapisan epidermis, mesofil, keberadaan kristal dan karakter khusus spesies serta bentuk berkas pengangkut pada tulang daun dan tangkai daun. Berdasarkan preparat paradermal daun diperoleh tipe daun hipostomatik dengan tipe stomata umumnya parasitik dan anomositik, dan ditemukan variasi tipe stomata anisositik pada Baccaurea nanihua dan Antidesma excavatum. Pada preparat transversal diperoleh tipe daun dorsiventral, bentuk epidermis dan jaringan tiang yang beragam. Pada organ tangkai daun, ditemukan empat tipe berkas pengangkut, yaitu bentuk lonjong dengan dua tambahan berkas pengangkut, bentuk dasar menyerupai ginjal, bentuk semi-lunar, dan bentuk lonjong dengan satu berkas pengangkut. Abstract Morphological characters are commonly used as a tool for plant identification. Anatomical characters can also be used as additional characters to provide strong descriptions of morphological characters and to resolve unclear identification of morphological characters. This study aims to identify leaf anatomical characters of 11 species of Phyllanthaceae family collected from the Banggai Kepulauan Regency. The characters are observed in each slide were paradermal characters, namely epidermis and its derivatives; transverse characters including the shape and number of epidermal layers, mesophyll, presence of crystals and species-specific characters as well as the shape of the vascular bundle on the midrib and petiole. The observation on paradermal section of lamina showed that all species have hypostomatic leaf, parasitic and anomocytic stomata types with variation of the anisocytic types were found in Baccaurea nanihua and Antidesma excavatum.Observation of the transverse section showed dorsiventral leaf types, size variation of upper epidermal cells as well as variations of palisade cells. The observation on transverse section of the petiole showed four types of vascular bundles in the petiole: oval shape along with two small separated vascular, the kidney – like shape, the semi-lunar shape and oval single vascular bundle.

Author(s):  
Nery Sofiyanti ◽  
DYAH IRIANI Iriani ◽  
FITMAWATI Fitmawati

Abstract. Sofiyanti N, Iriani D, Fitmawati. 2019. The first record of grass-like fern, Schizaea dichotoma (L.) J. Sm. (Schizaeaceae) from Lingga Island, Indonesia: Its morphological, anatomical and palynological study. Biodiversitas 20: 2651-2660. Schizaea dichotoma (L.) J.Sm. is one of fern species from Family Schizaeaceae. This species is grass-like, dimorphic with fan-shaped fronds, and well-known with local name Rumput Merak. The first record of this species is reported from Lingga Island, Riau Islands Province, Indonesia. The aim of this study was to examine the morphology, anatomy and spore characteristics of this fern. This study had been carried from June to December 2017. Samples were collected using exploration method, documented, and prepared for herbarium specimen. In this study, we examined the morphological characters, anatomical characters of rhizome, stipes, and pinnae of both fertile and sterile fronds, as well as spore feature. The anatomical preparations were carried out using paraffin method, while spore specimens using acetolysis method. The anatomy of rhizome, stipes, and pinnae, as well as spore characters, were then observed and documented using digital microscope Celeron. S. dichotoma is dimorphic fern with grass-like habit, fan-shaped laminae that repeatedly forked end in sorospore in fertile frond. The rhizome has vascular bundle surrounded by star-shaped sclerenchymatous layer. The cross-section of stipes of both fertile and sterile fronds are "M" shaped with a vascular bundle located in the dorsal side, while the leaves have a vascular bundle in the middle of midrib. The type of vascular bundles and stele of those organs are similar, i.a. collateral closed and protostele. We observed The Casparian strip in the endodermis layer of rhizome, stipes, and leaves, as well as at cortex and among the xylem. The spore of S. dichotoma is monolete and large spore class (ca. 59.5 ± 2.4 x 35.86 ± 2.01 µm).


Author(s):  
Saleem E. Shahbaz ◽  
Nazar M. Shareef

Introduction: Paliurus spina-christi Mill is a species with two varieties namely Paliurus spina-christi L. var. spina-christi and Paliurus spina-christivar. macrocarpa Beck native to mountains of Kurdistan, rarely growing in the upper plains of northern Iraq. Materials and Methods: A total of 15 plants from different parts of Kurdistan region were sampled. 30measurements for each characters of Leaf, Inflorescence, Flowers, Fruit, seeds, and leaf anatomical characters were measured for comparison between the two varieties. Results and Discussion: Fruit diameter of var. macrocarpa is significantly larger than fruit diameter of var. spina-christi. Most mean flower parts especially the ovary, in addition to seed size is larger in var. macrocarpa. Upper epidermal layer is always thicker than the lower epidermal layer for the same blade. The isobilateral mesophyll includes 2 layers of long palisade cells adaxially and 2-3 layers of short cells abaxially. Many vascular bundles are seen equally spaced in the mesophyll tissue.With the exception of the presence of simple hairs on the petiole adaxial side of the var. spina-cristi and the absence of these hairs from the same site of var. macrocarpa, all other anatomical features are considered to be of no taxonomic application. Stomatal density of the adaxial leaf side is up to 19 stomata per mm² in var. spina-christi while it is up to 38 stomata per mm² in var. macrocarpa. Conclusions: Both fruit diameter and the stomatal density of the adaxial leaf side constitute diagnostic characters for separating between the two varieties of Paliurus spina-christi Mill


2016 ◽  
Vol 50 (2) ◽  
Author(s):  
Mohamed M. Moawed

<italic>Medicago sativa</italic> L. (alfalfa) is one of the most important legume forages in the world. The objective of this study was to characterize and discriminate among 15 alfalfa cultivars with a different geographical origin. Macro-morphological and anatomical characters as well as seed coat sculpture were investigated. Twenty five morphological characters were extracted directly from the fresh specimens. Transverse section in the main stem were carried out; stained and seventeen anatomical characters were examined by light microscope. Seed coat surface was investigated using Scanning Electron Microscope (SEM). Data obtained were coded and analysed using NTsys-Pc software (Version 2.02) and the resulted dendrogram is discussed. The results showed morphological and anatomical variation between the studied taxa. Vascular bundles ranged from 16 to 23. The Egyptian cultivar Nubaria has the lowest number of vessels (16) while the American Super supreme has the larger number (23). The seed coat ornamentation revealed five main surface patterns and suggests the presence of variations in anticlinal boundaries and periclinal walls that provide stable diagnostic characters for morphologically closely related taxa. The dendrogram showed that the Egyptian cultivar Nubaria was the most distant and clustered separately from all the other alfalfa cultivars which were grouped into two main clusters. Seed coat morphology and combination of other plant morphological and anatomical characters permitted identification and discrimination between the examined cultivars. Results obtained in this work could be considered for further breeding strategies and studies.


2018 ◽  
Vol 20 (1) ◽  
pp. 327-336
Author(s):  
NERY SOFIYANTI ◽  
DYAH IRIANI ◽  
FITMAWATI FITMAWATI ◽  
AFNI ATIKA MARPAUNG

Sofiyanti N, Iriani D, Fitmawati, Marpaung AA. 2019. Morphology, palynology, and stipe anatomy of four common ferns from Pekanbaru, Riau Province, Indonesia. Biodiversitas 20: 327-336. The fern explorations had been conducted in Pekanbaru, Riau Province from 2013 to 2017. The objectives of this study were to characterize the morphological characters, to examine the spore features and stipe anatomy characters of four common ferns from Pekanbaru. The specimens were collected from the fields using the exploration method from 21 study sites from 10 sub-districts. The spores were then observed using a digital microscope. A total of four fern species were known as the most common fern species in this region, i.e., Dicranopteris linearis, Stenochlaena palustris, Nephrolepis biserrata, and Davalia denticulata. This study examined the morphology, palynology and stipe anatomy of these four common ferns from Pekanbaru, Riau. The anatomical preparation was conducted using paraffin method while spore preparation using acetolysis method. We found dimorphic ferns (Stenochlaena palustris and Davallia denticulata) and monomorphic ferns (Dicranopteris linearis and Nephrolepis biserrata). The vascular bundle of stipes of four species examined is similar, i.e., amphicribral concentric, however, the vascular bundles within the species varies in size. We also observed similar type of stele, atactostele, among the species. The spore features found in this study were monolete and trilete with prolate and subprolate shape. The result of this study supports the anatomical data of the examined species.


2020 ◽  
pp. 182-186
Author(s):  
G. Prabhakar ◽  
K. Shailaja ◽  
P. Kamalakar

The paper deals with a detailed investigation on the leaves of Maerua oblongifolia (Forssk.) A. Rich. which includes it’s morphological, anatomical and powder analysis. It is a low woody bushy under-shrub belonging to the family Capparaceae. The leaves are used in treatment of as fever, ear ache, stomach ache, skin infections, urinary calculii, diabetes mellitus, epilepsy, rigidity in lower limbs, and abdominal colic. The leaf amphistomatic, with mostly anamocytic, few tetracytic stomata. In transverse section of leaf is ribbed on either sides at midvein, epidermis one layered. Mesophyll differentiated into palisade and spongy tissues. Ground tissue of midvein differentiated into palisade, collenchyma and parenchyma. The midvien consists of one large oval shaped vascular bundles arranged are at the centre. Petiole in transverse section is circular adaxially small, grooved at centre and epidermis is having one layered, a single circular vascular bundle present at the centre, vascular bundle is enclosed by endodermis. The powder microscopic and organoleptic characters are also presented in this study. This study would helps as an appropriate source for authentification of the present studied drug.  


2013 ◽  
Vol 20 (2) ◽  
pp. 135-144 ◽  
Author(s):  
Lin Meng ◽  
Peichun Mao

The micromorphological and anatomical characters of Elytrigia caespitosa (K.Koch) Nevski , E. intermedia (Host) Nevski × E. elongata (Host) Nevski, E. intermedia(Host) Nevski and  E. repens (L.) Desv. ex Nevski have been studied using Scanning Electron Microscope (SEM) to determine interspecific variation. The results show that the root transverse section consists of epidermis, cortex and stele. Two rings of vascular bundles and a central pith cavity appear in stem morphology. The leaves of  E. caespitosa have either single or twin, horse shoe-shaped short cells born along the costal zone of the upper epidermis, which lack prickle hairs and contain spherical or oblique-shaped papillae. In  E. intermedia, the parallel subsidiary cells are distributed on the upper epidermis, and there are no short cells in the leaves. Dome-shaped subsidiary cells appear on the upper epidermis of  E. intermedia × E. elongata and E. repens, but E. intermedia × E. elongata showes spot-shaped papillae, and its bulliform cells sank into the “hinge cells”. E. repens has no papillae, and its bulliform cells are not sunken into the mesophyll. Therefore, the differences in micromorphological characters on the upper epidermis of the leaf could be useful in classifying and determining phylogenetic relationships among the species.DOI: http://dx.doi.org/10.3329/bjpt.v20i2.17388Bangladesh J. Plant Taxon.  20(2): 135-144, 2013


Author(s):  
Patil Kalpana ◽  
Wadekar Raju

To study the morpho-anatomy of the aerial parts of Trichosanthes lobata (Cucurbitaceae) to increase the knowledge and standardization parameters of these plants. Morpho-anatomical studies of leaves have been carried out by free hand. The different types of histochemical test were performed by using staining reagents. Phytochemical and quantitative estimation has been determined along with HPTLC fingerprinting. Leaves simple, reniform, or ovate, 5 lobbed and glabrous. Anomocytic stomata are present on lower epidermis. A single layer of elongated palisade cells are present below upper epidermis. Leaf bears covering trichomes unicellular to 3-4 celled long. The central region is occupied by arc shaped vascular bundles comprising of xylem vessels surrounded with phloem cells. Physicochemical paradigms such as; ash value, inorganic elements, moisture content and extractive values were determined to develop stringent Pharmacognostic standards. Qualitative, and Quantitative standardization and, HPTLC fingerprint study of alcoholic and aqueous extracts confirmed the presence of quercitin as biomarker polyphenolic compound. These studies provides referential information for correct identification, as well as assessment of purity, quality of this plant, which definitely gaining the relevance in plant drug research and establishment of plant monograph.


2019 ◽  
Vol 49 (1) ◽  
pp. 28-35
Author(s):  
Andréia Silva FLORES ◽  
Eduardo de Souza COSTA ◽  
Germana Bueno DIAS

ABSTRACT Comparative studies on the structure of foliar anatomy in four species of Rhynchosia from Roraima state (northern Brazilian Amazon) were carried out to identify additional morphological characters to support the definition of the systematic boundaries among the species. Fully expanded leaves, including the petioles, were collected from the upper nodes of a plant’s stem. Anatomical characters were observed in cross-sections of the middle portion of leaflets and petiole segments. Presence and distribution of glandular trichomes, continuity of sclerenchyma around the vascular bundles in leaflet blades and the conformation of the area between the two ridges of petioles were important characters to distinguish among the species of Rhynchosia. For the first time we report a distinct multicellular gland-like structure which we found in the petioles of three species.


2017 ◽  
Vol 48 (3) ◽  
pp. 112-121 ◽  
Author(s):  
H. Kraehmer

AbstractGrass nodes play an essential role as interfaces between leaf and stem. The description of the bundle course in nodes considerably contributes to understanding of the transport of assimilates, minerals, and xenobiotics in grasses. Nodes and internodes of 38 species of the subfamilies Arundinoideae, Bambusoideae, Panicoideae, and Pooideae were analyzed histologically. Free-hand sections, various staining techniques, macro- and microphotography were used to reveal a few principles underlying their anatomy. In all grass species, specific nodal zones were found in which many vascular bundles undergo characteristic transformations. This transformation starts with the augmentation of xylem in lower nodal areas and continues with the formation of specific amphivasal structures providing connections with the leaf attached to the node. The anatomy of these strands, herein called vasotubuli, has not much in common with vascular bundles in internodes any more. Transverse nodal plexus strands provide many connections between bundles and vasotubuli. The nodal plexus is also an interface of sclerenchyma bundles. The nodes of most grass species are constructed very similarly with a few exceptions: the nodes ofPhragmites australis(Cav.) Steud. for example have something in common with bamboo: they develop spindle-like glomeruli.


2021 ◽  
Vol 22 (3) ◽  
Author(s):  
Weereesa Boonthasak ◽  
CHATCHAI NGERNSAENGSARUAY

Abstract. Boonthasak W, Ngernsaengsaruay C. 2021. The genus Dolichandrone (Fenzl.) Seem. (Bignoniaceae) in Thailand. Biodiversitas 22: 1120-1128. Morphological, anatomical, and palynological studies of the genus Dolichandrone (Bignoniaceae) Thailand were conducted. Three species, D. columnaris Santisuk, D. serrulata (Wall. ex DC.) Seem., and D. spathacea (L. f.) Seem. were investigated. Morphological descriptions, distributions and ecological information are provided. A key to the species based on morphological characters are leaflet margins, length of lower cylindric tube and upper campanulate tube of corolla, width of upper campanulate tube of corolla, winged seed, shape and characters of fruits, width of septum, characters and width of pseudoseptum. D. columnaris occurs in low-lying rice fields and marshlands only in the peninsular region. D. serrulata occurs in mixed deciduous forest and low-lying rice fields in the eastern, central and peninsular regions. D. spathacea occurs in edges of mangrove forest in the central, south-eastern and peninsular regions. A key to the species based on anatomical characters includes leaf type, number of rows of palisade cells, arrangement of axial parenchyma, and height of ray parenchyma. All pollen grains are similar and do not provide characters for identification within the genus Dolichandrone.


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