scholarly journals A Novel Real-Time DNA Detection System for Loop-Mediated Isothermal Amplification Method

2010 ◽  
Vol 130 (5) ◽  
pp. 807-812
Author(s):  
Koji Kakugawa ◽  
Kenji Yamada ◽  
Hiroshi Maeda ◽  
Shougo Takashiba
Keyword(s):  
Real Time ◽  
Detection System ◽  
Dna Detection ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Method

scholarly journals Determination of ABO blood group genotypes using the real-time loop-mediated isothermal amplification method

2015 ◽  
Vol 12 (4) ◽  
pp. 5963-5966 ◽  
Author(s):  
CHAO ZHANG ◽  
JUANLI ZHU ◽  
JIANGCUN YANG ◽  
YINSHENG WAN ◽  
TING MA ◽  
...  
Keyword(s):  
Real Time ◽  
Blood Group ◽  
Isothermal Amplification ◽  
Abo Blood Group ◽  
The Real ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Method ◽  
Time Loop

scholarly journals Portable and label-free quantitative Loop-mediated Isothermal Amplification (qLAMP) for reliable COVID-19 diagnostics in 3 minutes: An Arduino-based detection system assisted by a pH microelectrode

2021 ◽  
Author(s):  
Mario Moisés Alvarez ◽  
Sergio Bravo-González ◽  
Everardo González-González ◽  
Grissel Trujillo-de Santiago
Keyword(s):  
Real Time ◽  
Detection System ◽  
Genetic Material ◽  
Cost Effective ◽  
Isothermal Amplification ◽  
Diagnostic Methods ◽  
Label Free ◽  
Viral Pathogens ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification System

Loop-mediated isothermal amplification (LAMP) has been recently studied as an alternative method for cost-effective diagnostics in the context of the current COVID-19 pandemic. Recent reports document that LAMP-based diagnostic methods have a comparable sensitivity and specificity to that of RT-qPCR. We report the use of a portable Arduino-based LAMP-based amplification system assisted by pH microelectrodes for the accurate and reliable diagnosis of SARS-CoV-2 during the first 3 minutes of the amplification reaction. We show that this simple system enables a straightforward discrimination between samples containing or not containing artificial SARS-CoV-2 genetic material in the range of 10 to 10,000 copies per 50 μL of reaction mix. We also spiked saliva samples with SARS-CoV-2 synthetic material and corroborated that the LAMP reaction can be successfully monitored in real time using microelectrodes in saliva samples as well. These results may have profound implications for the design of real-time and portable quantitative systems for the reliable detection of viral pathogens including SARS-CoV-2.

Development of a real time loop-mediated isothermal amplification method for detection of Senecavirus A

2018 ◽  
Vol 261 ◽  
pp. 98-103 ◽  
Author(s):  
Fanwen Zeng ◽  
Feng Cong ◽  
Xiangnan Liu ◽  
Yuexiao Lian ◽  
Miaoli Wu ◽  
...  
Keyword(s):  
Real Time ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Method ◽  
Time Loop

scholarly journals Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9278 ◽  
Author(s):  
Yee Ling Lau ◽  
Ilyiana Ismail ◽  
Nur Izati Mustapa ◽  
Meng Yee Lai ◽  
Tuan Suhaila Tuan Soh ◽  
...  
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
Clinical Sensitivity ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Method ◽  
Resource Limited ◽  
Highly Sensitive ◽  
Comparable Performance

Background Highly sensitive real-time reverse transcription polymerase chain reaction (RT-qPCR) methods have been developed for the detection of SARS-CoV-2. However, they are costly. Loop-mediated isothermal amplification (LAMP) assay has emerged as a novel alternative isothermal amplification method for the detection of nucleic acid. Methods A rapid, sensitive and specific real-time reverse transcription LAMP (RT-LAMP) assay was developed for SARS-CoV-2 detection. Results This assay detected one copy/reaction of SARS-CoV-2 RNA in 30 min. Both the clinical sensitivity and specificity of this assay were 100%. The RT-LAMP showed comparable performance with RT-qPCR. Combining simplicity and cost-effectiveness, this assay is therefore recommended for use in resource resource-limited settings.

Sign in / Sign up

Export Citation Format

Share Document