scholarly journals ID:2052 MANUFACTURING BIOLOGICAL MEMBRANE FROM BOVINE PERICARDIUM EXTRACELLULAR MATRIX TOWARD THE PERIODONTAL APPLICATION

2017 ◽  
Vol 4 (S) ◽  
pp. 82
Author(s):  
My Nguyen Thi Ngoc
Keyword(s):  
Biological Membrane ◽  
Human Fibroblasts ◽  
Bovine Pericardium ◽  
Membrane Properties ◽  
Collagen Membrane ◽  
Gingival Fibroblasts ◽  
In Vitro Degradation ◽  
In Vitro Biocompatibility ◽  
Porcine Pericardium

Collagen membrane that was mainly from the bovine and porcine pericardium has been studied and widely applied for most manufacturers of bioprothetic materials, especially periodontal therapeutics. As many studied, bovine pericardium has higher collagen content than that from porcine, although both membranes have no significant different in calcification when treated with glutaraldehyde. In this study, acellular bovine was used to fabricated a biomembrane for periodontal reconstruction and regeneration. Acellular bovine pericardium was treated with glutaraldehyde at different concentrations and time points. The treatment of 0,1% glutaraldehyde for 6 hours was determined as the optimal fabrication protocol according to membrane properties, including thickness (0.2mm-0.5mm), tensile strength (5MPa-12MPa), tensile strain (15%-50%), around 5µm pore size, limited in vitro degradation (1%-5%), which met all criteria parameters for periodontal treatment. The fabricated membrane presented in vitro biocompatibility to human fibroblasts according to ISO10993-5. Moreover, the membrane promoted the in vitro migration of humane gingival fibroblasts, which indicated the application as a guided biomembrane in periodontal reconstuction and regeneration.

Preliminary testing of silicone-urethane elastomers as substrates in human cell culture

e-Polymers ◽  
2007 ◽  
Vol 7 (1) ◽  
Author(s):  
Malgorzata Lewandowska-Szumieł ◽  
Janusz Kozakiewicz ◽  
Piotr Mrówka ◽  
Agnieszka Jurkowska ◽  
Edyta Sienkiewicz-Łatka ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Blood Platelets ◽  
Human Fibroblasts ◽  
Medical Implants ◽  
Tissue Engineering Scaffolds ◽  
Xtt Assay ◽  
In Vitro Biocompatibility ◽  
Different Types

AbstractSilicone-urethanes, polymers combining the characteristics of two widely used biomaterials, i.e. polyurethanes and silicones, are highly valued in many applications, including medical implants. To assess properties of these materials in contact with living cells, a set of different silicone-urethane materials, candidates for tissue engineering scaffolds, was synthesized and characterized. Two different oligomeric siloxane diols: Tegomer-2111 (Teg) and KF-6001 (KF), and two different types of diisocyanate, MDI and IPDI, were used in synthesis. Blood platelets adhesion to surfaces of selected materials showed a higher thrombogenicity of material based on Teg. Human fibroblasts were used in in vitro biocompatibility tests. The viability of cells cultured on silicone-urethanes was tested by XTT assay. Teg-based silicone-urethanes showed a significantly higher biocompatibility than those based on KF. Materials based on MDI compared to IPDI were found to be significantly more favoured by cells, not necessarily due to the type of diisocyanate but maybe also because of the necessity of using potentially toxic catalyst which accompanies the use of IPDI. Our studies indicate that silicone-urethanes are potent materials for tissue engineering products development. On the basis of the observations performed in cell culture, Tegomer- 2111 as oligomeric siloxane diol and MDI as diisocyanate are recommended as starting materials for silicone-urethane scaffolds synthesis.

scholarly journals In vitro Analysis of Cytotoxicity of Temporary Resilient Relining Materials

2016 ◽  
Vol 17 (6) ◽  
pp. 457-462 ◽  
Author(s):  
Isleine P Caldas ◽  
Miriam Z Scelza ◽  
Marco A Gallito ◽  
Gutemberg Alves ◽  
Licínio Silva
Keyword(s):  
Cell Viability ◽  
Human Fibroblasts ◽  
Primary Cultures ◽  
Polystyrene Latex ◽  
Test Method ◽  
Human Gingival Fibroblasts ◽  
Control Group ◽  
Gingival Fibroblasts ◽  
Significant Difference

ABSTRACT Aims The aim of this study is to evaluate the in vitro response of human gingival fibroblasts in primary cultures to two materials for temporary relining of dentures: Temporary Soft (TDV, Brazil) and Trusoft (Bosworth, USA) for 24 hours, 7 and 30 days by using a multi-parametric analysis. Materials and methods Each material sample (TDV, TS, Polystyrene, Latex) was prepared and incubated in a culture medium for 1, 7, and 30 days at 37°C. Human gingival fibroblasts were exposed to the extracts and cell viability was evaluated by a multi-parametric assay, which allowed sequential analysis of mitochondrial activity (XTT), membrane integrity [neutral red (NR)], and cell density [crystal violet dye exclusion (CVDE)] in the same cells. Analysis of variance (ANOVA) was used to test the interactions of the three sources of variation (material, test method, and time) with the proportions of viable cells for each relining material. Results Both evaluated materials (TDV and TS) had low cytotoxic effects during 1, 7, and 30 days after manipulation of the material, as assessed by all three methods used. A statistical difference was found when comparing the negative control group (latex fragments) with the other groups, which showed high toxicity and low percentage of cell viability in all tests used. There was no significant difference among other materials (p > 0.05). Conclusion Low cytotoxicity levels were detected by representatives of the major groups of temporary prosthetic relining materials, as evaluated by multiple cellular viability parameters in human fibroblasts. Clinical significance There are various soft materials on the market for relining prostheses; however, the effects of these materials on tissues need to be clarified to avoid problems for patients. How to cite this article Caldas IP, Scelza MZ, Gallito MA, Alves G, Silva L. In vitro Analysis of Cytotoxicity of Temporary Resilient Relining Materials. J Contemp Dent Pract 2016;17(6):457-462.

scholarly journals In vitro biocompatibility of biohybrid polymers membrane evaluated in human gingival fibroblasts

2020 ◽  
Vol 108 (6) ◽  
pp. 2590-2598 ◽  
Author(s):  
Bin Guo ◽  
Chuhua Tang ◽  
Mingguo Wang ◽  
Zhongqi Zhao ◽  
Hassan A. Shokoohi‐Tabrizi ◽  
...  
Keyword(s):  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
In Vitro Biocompatibility

scholarly journals Assessment of Human Gingival Fibroblast Proliferation After Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450 and 405nm) – Preliminary Report

2020 ◽  
Author(s):  
Barbara Sterczała ◽  
Kinga Grzech-Leśniak ◽  
Olga Michel ◽  
Witold Trzeciakowski ◽  
Kamil Jurczyszyn
Keyword(s):  
Energy Density ◽  
Preliminary Report ◽  
Human Fibroblasts ◽  
Mitochondrial Activity ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Proliferation ◽  
Gingival Fibroblasts ◽  
Healthy Donors

Purpose: to assess the effect of photobiomodulation (PBM) on human gingival fibroblast proliferation. Methods: The study was conducted using the primary cell cultures of human fibroblasts collected from systemically healthy donors. Three different laser types: Nd:YAG (1064nm), infrared diode laser (980nm) and prototype led laser emitting 405, 450 and 635nm were used to irradiate fibroblasts. Thanks to the patented structure of that laser, it was possible to irradiate fibroblasts with a beam combining two or three wavelengths. The energy density was 3 J/cm², 25 J/cm², 64 J/cm². The viability and proliferation of cells were determined using the MTT test conducted 24, 48 and 72 hours after laser irradiation. Results: The highest percentage of mitochondrial activity (MA=122.1%) was observed in the group irradiated with the 635nm laser, with an energy density of 64 J/cm² after 48 hours. The lowest percentage of MA (94.0%) was observed in the group simultaneously irradiated with three wavelengths (405 + 450 + 635 nm). The use of the 405nm laser at 25 J/cm² gave similar results to the 635 nm laser. Conclusions: The application of the 635nm and 405nm irradiation caused a statistically significant increase in the proliferation of gingival fibroblasts.

scholarly journals Bovine pericardium membrane, gingival stem cells, and ascorbic acid: a novel team in regenerative medicine

2019 ◽  
Vol 63 (3) ◽  
Author(s):  
Jacopo Pizzicannella ◽  
Guya D. Marconi ◽  
Sante D. Pierdomenico ◽  
Marcos F.X.B. Cavalcanti ◽  
Francesca Diomede ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ascorbic Acid ◽  
Regenerative Medicine ◽  
De Novo ◽  
Stem Cell Differentiation ◽  
Bovine Pericardium ◽  
Collagen Membrane ◽  
3D Scaffold ◽  
Related Transcription Factor

Recently, the development and the application of 3D scaffold able to promote stem cell differentiation represented an essential field of interest in regenerative medicine. In particular, functionalized scaffolds improve bone tissue formation and promote bone defects repair. This research aims to evaluate the role of ascorbic acid (AS) supplementation in an in vitro model, in which a novel 3D-scaffold, bovine pericardium collagen membrane called BioRipar (BioR) was functionalized with human Gingival Mesenchymal Stem Cells (hGMSCs). As extensively reported in the literature, AS is an essential antioxidant molecule involved in the extracellular matrix secretion and in the osteogenic induction. Specifically, hGMSCs were seeded on BioR and treated with 60 and 90 μg/mL of AS in order to assess their growth behavior, the expression of bone specific markers involved in osteogenesis (runt-related transcription factor 2, RUNX2; collagen1A1, COL1A1; osteopontin, OPN; bone morphogenetic protein2/4, BMP2/4), and de novo deposition of calcium. The expression of COL1A1, RUNX2, BMP2/4 and OPN was evaluated by RT-PCR, Western blotting and immunocytochemistry, and proved to be upregulated. Our results demonstrate that after three weeks of treatment AS at 60 and 90 μg/mL operates as an osteogenic inductor in hGMSCs. These data indicate that the AS supplementation produces an enhancement of osteogenic phenotype commitment in an in vitro environment. For this reason, AS could represent a valid support for basic and translational research in tissue engineering and regenerative medicine.

Effect of Modified Bovine Pericardium on Human Gingival Fibroblasts in vitro

2018 ◽  
Vol 206 (6) ◽  
pp. 296-307 ◽  
Author(s):  
My Thi Ngoc Nguyen ◽  
Ha Le Bao Tran
Keyword(s):  
Mechanical Properties ◽  
Periodontal Disease ◽  
Pore Size ◽  
Bovine Pericardium ◽  
Human Gingival Fibroblasts ◽  
Periodontal Treatment ◽  
Type I ◽  
Gingival Fibroblasts ◽  
Disease Treatment

Supportive membranes have recently been applied to treat periodontal disease in order to achieve periodontal tissue regeneration. The crucial role of these membranes is to facilitate the restoration of the structural and functional periodontium. Bovine pericardium (BP) is mainly composed of collagen type I, which was demonstrated to have good mechanical properties and biological regenerative potential. Our research aimed to extend the application of membrane derived from BP to periodontal disease treatment. However, the fabrication method to achieve a xenogenic-free membrane with the mechanical properties required for periodontal treatment is rarely mentioned. Therefore, a procedure for the extraction and modification of BP using sodium dodecyl sulfate (SDS) and glutaraldehyde (GA) was developed. BP was harvested and decellularized using different SDS concentrations (0.05–0.3%). GA was used to further modify the membranes to achieve suitable thickness, mechanical strength, and pore size. A combination protocol of 0.15% SDS treatment for 12 h with continuous agitation combined with 0.1% GA for 6 h for membrane fabricating was applied. The modified BP (mBP) had the targeted characteristics, such as 0.2–0.5 mm thickness, approximately 10 MPa in tensile strength, 30% in strain force, and a pore size <5 µm, which is comparable to commercially available collagen membranes. Findings from this study demonstrated that the established method was effective in preparing BP membrane for periodontal treatment while decreasing the concentration of reagents and processing time. Moreover, our modified membrane was found to have no cytotoxicity but supports the migration, attachment, and proliferation of human gingival fibroblasts in vitro. Taken together, these results confirmed that mBP is suitable for application in periodontal disease treatment and regeneration.

In vitro biocompatibility of air-fired opaque porcelain with human gingival fibroblasts

1988 ◽  
Vol 59 (2) ◽  
pp. 187-194 ◽  
Author(s):  
Charles M. Cobb ◽  
Connie L. White ◽  
Robert D. Gillahan ◽  
Daniel E. Tira
Keyword(s):  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
In Vitro Biocompatibility
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