scholarly journals Expression of P16 biomarker in squamous cell carcinoma of uterine cervix and its association with clinico-pathological parameters: A cross-sectional study

2020 ◽  
Vol 7 (9) ◽  
pp. 3954-3961
Author(s):  
Kalyani R. ◽  
Raghuveer C.V. ◽  
Sheela S.R.
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Uterine Cervix ◽  
Surrogate Marker ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Stage Of Disease ◽  
P16 Expression

Introduction: Cervical cancer is the most common cancer among females. P16 is the surrogate marker for cervical carcinoma. This study aimed to evaluate the association of P16 marker with clinic-pathological parameters in squamous cell carcinoma of uterine cervix. Methods: This was a cross-sectional study. Histological confirmed cases of squamous cell carcinoma (SCC) of cervix were considered. All cases were evaluated for IHC P16 expression as per lower anogenital squamous terminology (LAST) criteria and correlated with clinico-pathological parameters. The data was analyzed by SPSS software version 22. Results: Out of 75 cases, P16 biomarker expression was block positive, ambiguous and negative in 67 (89.3%), 5 (6.6%), and 3 (4%) cases, respectively. There was a significant association between P16 expression and age (p = 0.005). All cases between 30-59 years of age showed block positivity. There was no significant association between P16 expression and age at marriage (p = 0.951), age at menopause (p = 0.311), parity (p = 0.554), clinical symptoms/signs, stage of disease (p = 0.28), or histopathological grade (p = 0.877). Maximum expression was seen between 40-44 years. Moreover, all cases having 1 & 2 parity showed block positivity and all stage I cases showed block positivity. Conclusion: P16 biomarker was significantly expressed in cervical cancers of the relatively younger age group and those with early stage of disease.

scholarly journals Expression of p16 and Galectin3 in Squamous Cell Carcinoma of Uterine Cervix, in Relation to the Histomorphological Variants: A Cross-sectional Analysis

2021 ◽  
Author(s):  
Sarbashis Hota ◽  
Tushar Kanti Das
Keyword(s):  
Squamous Cell Carcinoma ◽  
High Risk ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Uterine Cervix ◽  
Tumour Microenvironment ◽  
Hpv Infection ◽  
Tumour Grade ◽  
Cross Sectional ◽  
P16 Expression

Introduction: Cancer of uterine cervix comprises a big chunk of cancer registration worldwide. Now-a-days the immunohistochemical marker p16 has emerged as the surrogate marker of high risk Human Papilloma Virus (HPV) infection in cervical tissue. Galectin3, a ubiquitous agent likely to modulate different pro-survival properties necessary for neoplastic cells, is recently emerging as the guardian of tumour microenvironment. Aim: To study the expression of p16 and galectin3 in different histomorphological variants of cervical Squamous Cell Carcinoma (SCC) and their association with grade and stage. Materials and Methods: An observational cross-sectional study was undertaken in the Department of Pathology in a tertiary care hospital in East India, from January, 2019 to June, 2020. Fifty three samples diagnosed as invasive Squamous Cell Carcinoma (SCC) of uterine cervix were taken by systematic random sampling. Immunohistochemical examination was done using monoclonal antibodies against p16 and galectin3 after obtaining thin sections from formalin fixed paraffin embedded blocks and retrieval of antigen. The data was interpreted by light microscopy using a semiquantitative method with respect to prefixed parameters and statistical analysis was done by chi-square test and Fisher’s exact test using SPSS version 25.0. Results: Fifty two out of fifty three cases (98.1%) of squamous cell carcinoma in this study showed almost 100% block posivity of p16 in the tumour cells -strongly corroborative with high risk HPV infection. The non-keratinizing and the basaloid variant showed the strongest intensity of staining (3+). Only one case showed complete negativity of p16 expression. In galectin3 positive cases, strong expression of this marker is found in the invasive tongues of the tumour cells at the junction of tumour stromal interface, consistent with our knowledge regarding the importance of galectin3 in regulating the tumour microenvironment. The strongest galectin3 positivity(3+) was found in the single case of Lymphoepithelioma like squamous cell carcinoma and showed almost 100% positivity among the neoplastic cell population; whereas the non-keratinizing and Basaloid variant showed almost negative expression. Significant association (p=0.00021) found between tumour grade and p16 intensity. Conclusion: The non-keratinizing and basaloid variants of squamous cell carcinoma have shown statistically significant association with highest intensity of p16 staining along with diminished expression of galectin3. Increased tumour grade is also significantly associated with strong staining intensity of p16 and decreased galectin3 expression. However, no significant association is found between galectin3 expression or intensity of p16 expression and the stage of tumour.

Prognostic potential of CK-19 managing disseminated oral squamous cell carcinomas in Pakistan: A descriptive cross-sectional study

2020 ◽  
pp. 1-13
Author(s):  
Waqas Iqbal ◽  
Uzma Tariq ◽  
Arhama Surwaich ◽  
Surwaich Ali channa ◽  
Abdul Majid ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cross Sectional Study ◽  
Squamous Cell Carcinomas ◽  
Sectional Study ◽  
College Hospital ◽  
Cross Sectional ◽  
Oral Squamous Cell Carcinomas

Abstract A cross-sectional study was conducted to assess the adoptability of CK-19 as a routine diagnostic assay and potential prognostic marker following disseminated oral squamous cell carcinoma in Pakistani population. The current descriptive study was conducted at Isra Dental College Hospital, Isra University, Hyderabad, Pakistan. Suspected patients of oral squamous cell carcinomas (OSCC), who visited the Isra Dental College Hospital’s outpatient department from January 2014 up to January 2015 with four year follow up (from January 2015 up to December 2019), were included after ethical approval of the Institutional board. SPSS version 21.0 was used for data analysis. Sixty cases of oral squamous cell carcinoma (OSCC) were selected for CK-19 quantification by using PCR before and after incisional biopsy.    

scholarly journals Frequency and Determinants of Tongue Lesions among Patients Reported in a Teaching Institute: A Cross-Sectional Study

2021 ◽  
pp. 59-68
Author(s):  
Mehwish Feroz Ali ◽  
Gulrukh Askary ◽  
Shahrukh Saran ◽  
Adnan Zaidi ◽  
Farwa Sajjad ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Study Design ◽  
Squamous Cell ◽  
Oral Submucous Fibrosis ◽  
Cross Sectional Study ◽  
Oral Lesions ◽  
Sectional Study ◽  
Cross Sectional

Aims: The purpose of the study is to evaluate the frequency, distribution and determinants of tongue lesions in our teaching institute. Also to find an association of common tongue lesions with various study variables. Study Design: Cross-sectional study design. Place and Duration of Study: The study was conducted at Fatima Jinnah Dental College & Hospital, Karachi, Pakistan. All the lesions presented on the tongue were included reported from January 2017 to December 2020. Methodology: There were 670 oral lesions documented in the Department of Oral Pathology and Oral Medicine out of which 93 (13.8%) represented tongue lesions. We included 93 patients with tongue lesions (45 men, 48 women; age range 18-80 years). These lesions include the atrophic tongue, geographic tongue, candidiasis, keratotic lesion, ulceration, oral pigmentation, fibroma, black hairy tongue, traumatic neuroma, herpes infection, oral submucous fibrosis, and oral squamous cell carcinoma. Results: In the study, common clinical presentations on the tongue were ulceration (29%), erythematous/depapillated tongue (22.6%), white keratotic and plaque-like lesions (21.6%), and black discoloration (8.6%). The clinical presentation was statistically associated with gender (p=0.03), age (p=0.04) and site of lesion (p<.001). Atrophic glossitis (19.4%), traumatic ulcer (12.9%), pseudomembranous candidiasis (12.9%), oral pigmentation (8.6%), oral squamous cell carcinoma (7.5%), geographic tongue (6.5%), recurrent aphthous stomatitis (6.5%), and frictional keratosis (6.5%) were most commonly reported tongue lesions. There was a relationship of tongue lesions with causes and site of the lesion (P<0.001). Conclusion: Initial tongue lesions may reflect underlying massive abnormal changes and this could be an early diagnostic parameter.  Through vigilant screening of the oral mucosa, we may be able to detect such mucosal alterations and search out the possible cause in order to provide effective treatment to the patient. In this way, we may also prevent the malignant transformation of any susceptible oral lesions.

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