scholarly journals Мікробоценози стічних вод Львова на різних етапах очищення

2013 ◽  
Vol 4 (2) ◽  
pp. 76-80
Author(s):  
K.V. Sholiak ◽  
S.О. Hnatush ◽  
T.B. Peretyatko ◽  
S.P. Gudz
Keyword(s):  
Wastewater Treatment ◽  
Treatment Plant ◽  
Selective Medium ◽  
Nitrifying Bacteria ◽  
Qualitative Composition ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Active Sludge ◽  
Resistant Strains ◽  
Reducing Bacteria

The aim of this work was to investigate some physiological groups of microorganisms which are components of wastewater microbiocenoses. Microorganisms were grown in Petri dishes containing 20–30 ml agar selective medium and in 25 ml tubes at a temperature +30 ºC. The selective media were: wort agar for microscopic fungi and yeasts, Hutchinson medium for the cellulose-destroying microorganisms, starch-ammonium medium for microorganisms that can utilize mineral nitrogen forms, Postgate B medium for sulfate-reducing bacteria, Vinogradsky medium for nitrifying bacteria, Ashby medium for the nitrogen-fixing bacteria, Chapek medium for the actinomycetes. 1 mM Cr (VI) (104 mg/l) in the form of К2Cr2О7 was added to the medium. The number of colonies was determined by the Koch method. We studied wastewater microbocenoses of Lviv city at various stages of purification. We showed that the quantitative and qualitative composition of microorganisms differed significantly in primary and secondary clarifiers, the aerotank and sludge at different stages of sewage treatment. In the initial stages of purification, in the primary sump, bacteria that reached the treatment plant with sewage were found. Nitrifying bacteria (7.1 × 106colony forming units (CFU)/ml), nitrogen-fixing bacteria (9.0 × 106CFU/ml), and fungi (3.4 × 106 CFU/ml) dominated. The qualitative composition of microorganisms in primary clarifiers and the aerotank was similar, but their number in the aerotank was significantly higher than in the primary sump: 1.5 × 107 CFU/ml of nitrifying bacteria, 1.4 × 107CFU/ml of nitrogen-fixing bacteria, 6.7 × 106CFU/ml of fungi. The ratio of different physiological groups of microorganisms in the active sludge changed significantly. The predominant microorganisms were those that assimilate mineral forms of nitrogen (65%), their number was 1.6 × 108CFU/ml. In the secondary clarifier, the largest group was cellulose-destroying microorganisms (6.0 × 105CFU/ml). However, their numbers in the secondary sump were lower compared to their numbers in the aerotank and sludge (1.5–3.9 × 106CFU/ml). Among the representatives of various physiological groups of bacteria a significant number of chromium-resistant strains was detected. The largest number of chromium-resistant strains was detected in the active sludge and aerotank, which is probably due to the recirculation of microorganisms in the wastewater treatment. The highest percentage of Cr (VI) resistant microorganisms was among sulphate-reducing bacteria. An increase in the percentage of chromium-resistant microorganisms occurred together with the lowering of the total number of microorganisms of a certain physiological group. These microorganisms could prove useful for the development of biotechnological methods wastewater treatment to eliminate chromium compounds, which are highly toxic to living organisms.

Impact of prehybridization PCR amplification on microarray detection of nitrifying bacteria in wastewater treatment plant samples

2006 ◽  
Vol 8 (9) ◽  
pp. 1564-1574 ◽  
Author(s):  
Slil Siripong ◽  
John J. Kelly ◽  
David A. Stahl ◽  
Bruce E. Rittmann
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Pcr Amplification ◽  
Nitrifying Bacteria ◽  
Plant Samples ◽  
Microarray Detection

scholarly journals Simulating the dispersion of poisonous organic chemical compounds in wastewater treatment process through the active sludge method using the TOXChem model

2021 ◽  
Vol 5 (1) ◽  
pp. 025-031
Author(s):  
Masoomi Seyyed Roohollah ◽  
Azizi Mostafa ◽  
Aghlmand Reza ◽  
Gheibi Mohammad ◽  
Kian Zahra
Keyword(s):  
Heavy Metals ◽  
Wastewater Treatment ◽  
Chemical Structure ◽  
Treatment Process ◽  
Treatment Plant ◽  
Chemical Compounds ◽  
Organic Chemical ◽  
Gaseous Pollutants ◽  
Active Sludge ◽  
Wastewater Treatment Process

Naturally, microorganisms decompose the organic material existing in nature, both in the presence or absence of oxygen. The majority of materials such as poisonous chemical compounds, heavy metals, would prevent the treatment process from taking place, lead to the entry of these contaminants into the environment results in the emergence of numerous diseases. In the present study, using the TOXChem4.1 simulation model, attempts were made to simulate a wastewater treatment plant and then assess the dispersions of contaminants including 1,2-Dimethylnaphthalene, 1,3-Dinitropyrene, 1,6-Dimethylnaphthalene, 1,6-Dinitropyrene, and 17a-ethinylestradiol (EE2) in concentrations of a common scenario. The results of computer simulations showed that the EE2 contaminant is of the highest percentage of decomposition among others, due to its wider chemical structure. Consequently, it is clear that such contaminant is of the highest mass in the sludge exiting the treatment plant. In addition, the results of the simulations demonstrated that the highest volumes of gaseous pollutants take place in the modulation and initial sedimentation units.

Bacterial Diversity of Active Sludge in Wastewater Treatment Plant

2008 ◽  
Vol 15 (6) ◽  
pp. 163-168 ◽  
Author(s):  
Xin JIANG ◽  
Mingchao MA ◽  
Jun LI ◽  
Anhuai LU ◽  
Zuoshen ZHONG
Keyword(s):  
Wastewater Treatment ◽  
Bacterial Diversity ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Active Sludge

Development of the Ammonia Biosensor Monitoring System

1993 ◽  
Vol 28 (11-12) ◽  
pp. 435-445 ◽  
Author(s):  
H. Tanaka ◽  
E. Nakamura ◽  
H. Hoshikawa ◽  
Y. Tanaka
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Phosphate Buffer Solution ◽  
Field Tests ◽  
Nitrifying Bacteria ◽  
Secondary Effluent ◽  
Buffer Solution ◽  
Biosensor System ◽  
Isothermal Water

The ammonia biosensor system was newly developed for monitoring ammonia in a river and in secondary effluent from a wastewater treatment plant. The detector of the ammonia biosensor is nitrifying bacteria immobilized by two acetic cellulose membranes and the transducer of the sensor is a dissolved oxygen probe. The microbial probe performs excellent selectivity and quick response to ammonia although control of pH with a phosphate buffer solution and control of temperature with an isothermal water bath are required. The developed ammonia biosensor system processes automatic calibration, sampling, and measurement as a monitor in the field. Accuracy and reliability of the biosensor system were also demonstrated by field tests accomplished at a wastewater treatment plant and at a river quality monitoring station.

Enrichment of Nitrogen-Fixing Bacteria in a Nitrogen-Deficient Wastewater Treatment System

2020 ◽  
Vol 54 (6) ◽  
pp. 3539-3548 ◽  
Author(s):  
Carolina Ospina-Betancourth ◽  
Kishor Acharya ◽  
Ben Allen ◽  
Jim Entwistle ◽  
Ian M. Head ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Treatment System ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Wastewater Treatment System

Boosting nitrification with the BABE technology

2005 ◽  
Vol 52 (4) ◽  
pp. 63-70 ◽  
Author(s):  
D.H.J.G. Berends ◽  
S. Salem ◽  
H.F. van der Roest ◽  
M.C.M. van Loosdrecht
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Low Cost ◽  
Treatment Plant ◽  
Full Scale ◽  
Nitrifying Bacteria ◽  
Stable Operation ◽  
Main Process ◽  
Model Studies ◽  
Full Scale Tests

Over the past years there has been a growing interest for compact, simple, low cost and robust technologies to upgrade wastewater treatment plants for nitrogen removal. The BABE (Bio Augmentation Batch Enhanced) technology is such a new concept. This patented system for biological treatment of sludge liquor – the effluent produced from digested sludge – uses a new principle, boosting the nitrifying bacteria in a side stream in such a way that the activated sludge in the main process is augmented. This augmentation increases the nitrification capacity of the wastewater treatment plant (wwtp). Experiments on a practical scale have demonstrated the effective and stable operation of the BABE technology. Model studies supported by the results of the full-scale tests showed that the technology can be applied in several situations, i.e. 1) introducing nitrification at high loaded wwtps; 2) enhancing nitrification at wwtps with incomplete nitrification; 3) enlarging denitrification at wwtps with complete nitrification. Most likely this year a full-scale application will be realized in the Netherlands at a wwtp with insufficient nitrification throughout the year.

Development of Bacterial Consortia and Biodegradation Ability under Different PAH Stresses

2014 ◽  
Vol 1073-1076 ◽  
pp. 176-182
Author(s):  
Jing Ma ◽  
Shao Liang Zhang ◽  
Jun Feng Qu ◽  
Ai Hua Yan ◽  
Fu Chen
Keyword(s):  
Wastewater Treatment ◽  
Environmental Pollution ◽  
Wastewater Treatment Plant ◽  
Bacterial Communities ◽  
Treatment Plant ◽  
Species Structure ◽  
Active Sludge ◽  
Environmental Pressures ◽  
Bacterial Consortia ◽  
Degrading Bacteria

The aims of the present work were simulating actual environmental pollution to select and compare the bacterial communities under different environmental stresses such as phenanthrene and pyrene. Two bacterial consortia named as Phe consortium and Pyr consortium were enriched from activated sludge obtained from a wastewater treatment plant. The PCR-DGGE analysis showed that the original active sludge harbored abundantly diverse PAH-degrading bacteria at first, then under different environmental pressures the consortium species tended to be concentrated and had species structure differences. In addition, the abilities of the two bacterial consortia to remediate a mixture of 4 PAHs (50 mg Kg-1 each) in soil were studied. Of the 4 PAHs, greater than 80% of the fluorene and phenanthrene in soil were removed by the Phe consortium in just 7-days respectively; whereas the Pyr-consortium could degrade over 60% of anthracene and pyrene.

The impact of the controlled emptying of in-sewer storage on wastewater treatment plant performance

2002 ◽  
Vol 45 (3) ◽  
pp. 247-253 ◽  
Author(s):  
A.G. Jack ◽  
R.M. Ashley
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Effective Means ◽  
Treatment Plant ◽  
Combined Loading ◽  
Plant Performance ◽  
Nitrifying Bacteria ◽  
Reactor Performance ◽  
Regeneration Times ◽  
The Impact

The use of in-sewer storage is generally considered to be an effective means of minimising the effects of intermittent discharges into receiving watercourses during combined flows. Despite this, very little information is available about the consequential effects these flows may have on recipient wastewater treatment plant performance. Typical problems may include biomass washout (hydraulic), and reduced biological reactor performance due to dilute loading (biological). A study is described where detailed analysis was carried out to ascertain the consequential effects of prolonged dilute loading on an activated sludge wastewater treatment plant in Perth, Scotland. Consideration was given to likely storage volumes which may have been utilised in the catchment to resolve local problems. A comprehensive analysis of resulting treatment plant performance was carried out for variations in flow and various wet weather loadings. It is concluded that storage may cause little or no benefit with respect to ammonia total emissions due to reduced treatment of dry weather flows subsequent to the prolonged combined loading period. This was exacerbated by the long regeneration times of nitrifying bacteria. However, an overall benefit with respect to BOD total emissions would always be expected, as appropriately sized storage would retain the first foul flush at the CSOs, thereby compensating the increased emissions from the downstream wastewater treatment plant.

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