Development of Bacterial Consortia and Biodegradation Ability under Different PAH Stresses

2014 ◽  
Vol 1073-1076 ◽  
pp. 176-182
Author(s):  
Jing Ma ◽  
Shao Liang Zhang ◽  
Jun Feng Qu ◽  
Ai Hua Yan ◽  
Fu Chen
Keyword(s):  
Wastewater Treatment ◽  
Environmental Pollution ◽  
Wastewater Treatment Plant ◽  
Bacterial Communities ◽  
Treatment Plant ◽  
Species Structure ◽  
Active Sludge ◽  
Environmental Pressures ◽  
Bacterial Consortia ◽  
Degrading Bacteria

The aims of the present work were simulating actual environmental pollution to select and compare the bacterial communities under different environmental stresses such as phenanthrene and pyrene. Two bacterial consortia named as Phe consortium and Pyr consortium were enriched from activated sludge obtained from a wastewater treatment plant. The PCR-DGGE analysis showed that the original active sludge harbored abundantly diverse PAH-degrading bacteria at first, then under different environmental pressures the consortium species tended to be concentrated and had species structure differences. In addition, the abilities of the two bacterial consortia to remediate a mixture of 4 PAHs (50 mg Kg-1 each) in soil were studied. Of the 4 PAHs, greater than 80% of the fluorene and phenanthrene in soil were removed by the Phe consortium in just 7-days respectively; whereas the Pyr-consortium could degrade over 60% of anthracene and pyrene.

scholarly journals Characterization of the Spatial Variation of Microbial Communities in a Decentralized Subtropical Wastewater Treatment Plant Using Passive Methods

Water ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1157
Author(s):  
Marycarmen Verduzco Garibay ◽  
Alberto Fernández del Castillo ◽  
Osiris Díaz Torres ◽  
José de Anda ◽  
Carlos Yebra-Montes ◽  
...  
Keyword(s):  
Organic Matter ◽  
Wastewater Treatment ◽  
Spatial Variation ◽  
Wastewater Treatment Plant ◽  
Bacterial Communities ◽  
Treatment Plant ◽  
Oxygen Demand ◽  
Microbial Interactions ◽  
Nitrifying Bacteria ◽  
Degrading Bacteria

Septic tanks (STs), up-flow anaerobic filters (UAFs), and horizontal-flow constructed wetlands (HFCWs) are cost-effective wastewater treatment technologies especially efficient in tropical and sub-tropical regions. In this study, the bacterial communities within a decentralized wastewater treatment plant (WWTP) comprising a ST, a UAF, and a HFCW were analyzed using high-throughput sequencing of the V3–V4 region of the 16S rRNA gene. Bacterial diversity and its spatial variation were analyzed at the phylum and family level, and principal component analysis (PCA) was applied to nitrogen- and organic-matter-degrading families. The highest percentage of nitrogen removal was seen in the HFCW (28% of total Kjeldahl nitrogen, TKN, and 31% of NH3-N), and our results suggest that families such as Rhodocyclaceae (denitrifying bacteria), Nitrospiraceae (nitrifying bacteria), and Rhodospirillaceae (sulfur-oxidizing bacteria) contribute to such removal. The highest percentage of organic matter removal was seen in the UAF unit (40% of biological oxygen demand, BOD5, and 37% of chemical oxygen demand, COD), where organic-matter-degrading bacteria such as the Ruminococcaceae, Clostridiaceae, Lachnospiraceae, and Syntrophaceae families were identified. Redundancy analysis demonstrated that bacterial communities in the HFCW were more tolerant to physicochemical changes, while those in the ST and the UAF were highly influenced by dissolved oxygen and temperature. Also, pollutant removal pathways carried out by specific bacterial families and microbial interactions were elucidated. This study provides a detailed description of the bacterial communities present in a decentralized WWTP located in a subtropical region.

scholarly journals Isolation and characterization of organic matter-degrading bacteria from coking wastewater treatment plant

2018 ◽  
Vol 78 (7) ◽  
pp. 1517-1524 ◽  
Author(s):  
Riqiang Li ◽  
Jianxing Wang ◽  
Hongjiao Li
Keyword(s):  
Organic Matter ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Removal Rate ◽  
Treatment Plant ◽  
Coking Wastewater ◽  
Naphthalene Degradation ◽  
Isolation And Characterization ◽  
Degrading Bacteria

Abstract As a step toward bioaugmentation of coking wastewater treatment 45 bacteria strains were isolated from the activated sludge of a coking wastewater treatment plant (WWTP). Three strains identified as Bacillus cereus, Pseudomonas synxantha, and Pseudomonas pseudoaligenes exhibited high dehydrogenase activity which indicates a strong ability to degrade organic matter. Subsequently all three strains showed high naphthalene degradation abilities. Naphthalene is a refractory compound often found in coking wastewater. For B. cereus and P. synxantha the maximum naphthalene removal rates were 60.4% and 79.8%, respectively, at an initial naphthalene concentration of 80 mg/L, temperature of 30 °C, pH of 7, a bacteria concentration of 15% (V/V), and shaking speed of 160 r/min. For P. pseudoaligenes, the maximum naphthalene removal rate was 77.4% under similar conditions but at 35 °C.

scholarly journals Effect of Wastewater Treatment Plant Effluent on Microbial Function and Community Structure in the Sediment of a Freshwater Stream with Variable Seasonal Flow

2008 ◽  
Vol 74 (9) ◽  
pp. 2659-2668 ◽  
Author(s):  
Steven A. Wakelin ◽  
Matt J. Colloff ◽  
Rai S. Kookana
Keyword(s):  
Wastewater Treatment ◽  
Community Structure ◽  
Bacterial Community ◽  
16S Rrna ◽  
Wastewater Treatment Plant ◽  
Bacterial Communities ◽  
Nutrient Loading ◽  
Treatment Plant ◽  
Biogeochemical Cycling ◽  
Rrna Genes

ABSTRACT We investigated the effects of wastewater treatment plant (WWTP) discharge on the ecology of bacterial communities in the sediment of a small, low-gradient stream in South Australia. The quantification of genes involved in the biogeochemical cycling of carbon and nitrogen was used to assess potential impacts on ecosystem functions. The effects of disturbance on bacterial community structure were assessed by PCR-denaturing gradient gel electrophoresis of 16S rRNA genes, and clone library analysis was used to phylogenetically characterize significant shifts. Significant (P < 0.05) shifts in bacterial community structures were associated with alteration of the sediment's physicochemical properties, particularly nutrient loading from the WWTP discharge. The effects were greatest at the sampling location 400 m downstream of the outfall where the stream flow is reduced. This highly affected stretch of sediment contained representatives of the gammaproteobacteria that were absent from less-disturbed sites, including Oceanospirillales and Methylococcaceae. 16S rRNA gene sequences from less-disturbed sites had representatives of the Caulobacteraceae, Sphingomonadaceae, and Nitrospirae which were not represented in samples from disturbed sediment. The diversity was lowest at the reference site; it increased with proximity to the WWTP outfall and declined toward highly disturbed (400 m downstream) sites (P < 0.05). The potential for biological transformations of N varied significantly with the stream sediment location (P < 0.05). The abundance of amoA, narG, and nifH genes increased with the distance downstream of the outfall. These processes are driven by N and C availability, as well as redox conditions. Together these data suggest cause and effect between nutrient loading into the creek, shift in bacterial communities through habitat change, and alteration of capacity for biogeochemical cycling of N.

scholarly journals Key Success Factors Of Line Of Balance (LOB) And M-PERT Integration On The Development Of Wastewater Treatment Plant

2019 ◽  
Vol 8 (6) ◽  
pp. 986-990
Keyword(s):  
Wastewater Treatment ◽  
Environmental Pollution ◽  
Wastewater Treatment Plant ◽  
Success Factors ◽  
Treatment Plant ◽  
Domestic Wastewater ◽  
Influential Factors ◽  
City Government ◽  
Alternative Pathways ◽  
Statistical Analysis Method

The construction of a wastewater treatment plant in Indonesia to overcome domestic wastewater discharges has experienced many delays so that people are threatened not to enjoy development facilities that can protect them from the dangers of environmental pollution. The purpose of this paper is to analyze the application of the LOB and M-PERT scheduling methods by identifying the factors that influence the application of the two scheduling methods and how the results of the analysis are implemented A total of 40 questionnaires were distributed to consultant planners and supervisors of wastewater treatment plant and with the RII (Relative Importance Index) statistical analysis method it was found that the most influential factors were analysis of M-PERT activities in the form of combining activities in one group and on alternative pathways, and calculating reset PERT. Analysis of LOB implementation is in the form of optimizing project time, relationship logic activities, analysis of buffer time determination, and duration of work units. Schedule in the form of land ownership, inability to acquire required permission, and the influence of local custom and religious events. Based on these findings, the speakers recommend the following: 1) The planning consultant can socialize the application of the LOB and M-PERT methods to all stakeholders involved starting from contractors, field supervisors, and the local City Government; 2) Encouraging local City Government to be able to increase the number of wastewater treatment plant facilities built; 3) Increase the number of city residents who are protected from the dangers of environmental pollution with the wider scope of services provided.

scholarly journals Key Success Factors of Line of Balance (LOB) And M-PERT Integration on The Development of Wastewater Treatment Plant

2019 ◽  
Vol 8 (6) ◽  
pp. 3204-3208
Keyword(s):  
Wastewater Treatment ◽  
Environmental Pollution ◽  
Wastewater Treatment Plant ◽  
Success Factors ◽  
Treatment Plant ◽  
Domestic Wastewater ◽  
Influential Factors ◽  
City Government ◽  
Alternative Pathways ◽  
Statistical Analysis Method

The construction of a wastewater treatment plant in Indonesia to overcome domestic wastewater discharges has experienced many delays so that people are threatened not to enjoy development facilities that can protect them from the dangers of environmental pollution. The purpose of this paper is to analyze the application of the LOB and M-PERT scheduling methods by identifying the factors that influence the application of the two scheduling methods and how the results of the analysis are implemented A total of 40 questionnaires were distributed to consultant planners and supervisors of wastewater treatment plant and with the RII (Relative Importance Index) statistical analysis method it was found that the most influential factors were analysis of M-PERT activities in the form of combining activities in one group and on alternative pathways, and calculating reset PERT. Analysis of LOB implementation is in the form of optimizing project time, relationship logic activities, analysis of buffer time determination, and duration of work units. Schedule in the form of land ownership, inability to acquire required permission, and the influence of local custom and religious events. Based on these findings, the speakers recommend the following: 1) The planning consultant can socialize the application of the LOB and M-PERT methods to all stakeholders involved starting from contractors, field supervisors, and the local City Government; 2) Encouraging local City Government to be able to increase the number of wastewater treatment plant facilities built; 3) Increase the number of city residents who are protected from the dangers of environmental pollution with the wider scope of services provided.

Bacterial Diversity of Active Sludge in Wastewater Treatment Plant

2008 ◽  
Vol 15 (6) ◽  
pp. 163-168 ◽  
Author(s):  
Xin JIANG ◽  
Mingchao MA ◽  
Jun LI ◽  
Anhuai LU ◽  
Zuoshen ZHONG
Keyword(s):  
Wastewater Treatment ◽  
Bacterial Diversity ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Active Sludge
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