scholarly journals In vitro propagation of sacha inchi through organogenesis

2018 ◽  
Vol 53 (11) ◽  
pp. 1285-1288 ◽  
Author(s):  
Reynaldo Solis ◽  
Danter Cachique ◽  
Juan Carlos Guerrero-Abad ◽  
María Emilia Ruiz Sánchez ◽  
Lourdes Tapia y Figueroa
Keyword(s):  
In Vitro Propagation ◽  
Naphthaleneacetic Acid ◽  
Indolebutyric Acid ◽  
In Vitro Organogenesis ◽  
Apical Meristems ◽  
Hormonal Balance ◽  
Plukenetia Volubilis ◽  
Sacha Inchi

Abstract: The objective of this work was to evaluate hormonal balance in the phases of the in vitro organogenesis of apical meristems of sacha inchi (Plukenetia volubilis). For the establishment and multiplication phases, Murashige & Skoog (MS) media, with different concentrations of benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), were used. For rooting, modified MS media was supplemented with different concentrations of NAA and indolebutyric acid. The differentiation of apical meristems was possible with 0.1 mg L-1 BAP and 0.05 mg L-1 NAA. The best rooting of seedlings is obtained with 0.5 mg L-1 naphthaleneacetic acid and 2.0 mg L-1 indolebutyric acid.

scholarly journals First Report of Krymsk® 5 (cv. VSL 2) Cherry Rootstock In Vitro Propagation: Studying the Effect of Cytokinins, Auxins and Endogenous Sugars

2018 ◽  
Vol 47 (1) ◽  
pp. 152-161 ◽  
Author(s):  
Athanasios TSAFOUROS ◽  
Peter A. ROUSSOS
Keyword(s):  
Carbon Source ◽  
In Vitro Propagation ◽  
Carbon Sources ◽  
The Other ◽  
Mother Plant ◽  
Naphthaleneacetic Acid ◽  
Indolebutyric Acid ◽  
Synthetic Auxins ◽  
Number Of Shoots

Krymsk® 5 (VSL-2) is a semi-dwarf cherry rootstock adaptable to a range of climates. The present study aimed to establish the first efficient in vitro propagation protocol for this rootstock. Therefore, six cytokinines, four adenine type (6-benzyladenine, 2-isopentenyladenine, kinetin and meta-topolin) and two phenylureas (thidiazuron and forchlorfenuron) at three (2.4 μΜ, 4.8 μΜ and 9.6 μΜ) concentrations plus three (0.24 μΜ, 0.48 μΜ, 0.96 μΜ) for thidiazuron only were tested during the multiplication stage. 6-Benzyladenine was the most efficient cytokinin, based on the number of shoots produced (3.5 shoots at 9.6 μΜ) and the number of nodes per explant (10 nodes at 9.6 μΜ) whereas the other aromatic adenine tested, i.e. meta-topolin, presented the highest number of nodes per cm and node per shoot. During the rooting stage the synthetic auxins 1-naphthaleneacetic acid (1-NAA) and indolebutyric acid (IBA) were tested at concentrations of 0, 2.5, 5, 10 and 20 μΜ both separately and in all possible combinations. The percentage of successfully rooted explants reached 95% under the combination of 20 μΜ IBA plus 5 μΜ 1-NAA, whereas the highest number of roots recorded was 8.5 roots for the treatment 20 μΜ ΙΒΑ plus 2.5 μΜ 1-NAA. Furthermore, two different carbon sources were compared, the widely used sucrose and the endogenous sugar ratio of mother plant softwood shoot, sampled during late of May. Endogenous sugar ratio proved to be the preferable carbon source, since it increased the number of shoots produced and almost doubled the number of produced nodes per explant.

scholarly journals In vitro Propagation and Assessment of Genetic Relationships of Citrus Rootstocks Using ISSR Molecular Markers

2017 ◽  
Vol 45 (2) ◽  
pp. 383-391 ◽  
Author(s):  
Constantinos SALIS ◽  
Ioannis E. PAPADAKIS ◽  
Spyridon KINTZIOS ◽  
Marianna HAGIDIMITRIOU
Keyword(s):  
Molecular Markers ◽  
Genetic Variability ◽  
Root Length ◽  
In Vitro Propagation ◽  
Genetic Relationships ◽  
Shoot Proliferation ◽  
Poncirus Trifoliata ◽  
Naphthaleneacetic Acid ◽  
Issr Molecular Markers

The behavior of six citrus rootstocks, Volkameriana, Citrumelo ‘Swingle’, Citrange ‘Carrizo’, Poncirus trifoliata ‘Serra’, Poncirus trifoliata ‘Rubidoux’ and Poncirus trifoliata ‘Flying Dragon’, in in vitro propagation was studied and compared for shoot proliferation and rooting. In addition, the genetic relationships among the rootstocks studied and other Citrus species, using the Inter-Simple Sequence Repeats (ISSR) molecular markers, were investigated. Nodal explants of three months old shoots were used in Murashige and Skoog medium supplemented with N6-benzyladenine (BA) for shoot proliferation and with naphthaleneacetic acid (NAA) for rooting. The rootstock Volkameriana showed a statistically significant higher number of shoots (1.81), shoot length (15.14 mm) and number of leaves per explant (5.81), while all three Poncirus trifoliata rootstocks showed the lowest numbers. The number of roots and root length per explant were evaluated at the end of the rooting phase. The rootstock ‘Swingle’ showed a higher number of roots per explant (4.2) followed by ‘Flying Dragon’ (3.93) and ‘Carrizo’ (3.23) rootstocks. The rootstocks ‘Swingle’ (140.8 mm), Volkameriana (148 mm) and ‘Flying Dragon’ (131.12 mm) had significantly higher root length per explant compared to ‘Carrizo’ (31 mm) and ‘Rubidoux’ (34.5 mm). The ISSR molecular marker technique used in the present study grouped successfully the different species, varieties and rootstocks studied, revealing their genetic variability. The genetic variability observed among the rootstocks ranged between 0.29 (Poncirus trifoliata ‘Serra’ and Citrumelo ‘Swingle’) and 0.60 (Volkameriana and Citrumelo ‘Swingle’). The response of the rootstocks studied in in vitro propagation however is not related to their genetic affinity.

scholarly journals Desempenho horticultural de plantas propagadas in vitro de Sacha inchi

2014 ◽  
Vol 44 (6) ◽  
pp. 1050-1053 ◽  
Author(s):  
Paulo Hercílio Viegas Rodrigues ◽  
Stevan Ricardo Bordignon ◽  
Glaucia Maria Bovi Ambrosano
Keyword(s):  
Plukenetia Volubilis ◽  
Sacha Inchi

O objetivo do presente trabalho foi avaliar, em condições de campo, características horticulturais de mudas propagadas in vitro de Sacha inchi (Plukenetia volubilis L), quanto à época de florescimento e colheita, características morfológicas relacionadas à coloração e forma de folhas, caule e frutos, bem como a produtividade do material propagado in vitro. As mudas de cultura de tecidos foram propagadas por seis semanas em meio de cultivo com sais e vitaminas de MS, acrescido de 30g L-1 de sacarose e 1,0mg L-1 de BAP, a partir do ponteiro de plântula germinada in vitro. Foram selecionadas, ao acaso, quatro plantas propagadas in vitro, quatro plantas por via seminal e mais vinte e duas plantas, também por via seminal, compondo a bordadura, totalizando trinta plantas. As análises foram semanais até completar quatorze meses do plantio, que ocorreu antes da primeira poda. Os resultados obtidos indicam a não ocorrência de variabilidade genética nas plantas produzidas in vitro, bem como precocidade na produção e maior produtividade, quando comparado com o material convencional obtido via seminal.

scholarly journals Efeito inibitório de extrato alcoólico de amêndoa de Sacha-inchi em Rhizoctonia sp

2021 ◽  
Vol 14 (6) ◽  
pp. 27-30
Author(s):  
Renan Colavite Dos Santos ◽  
Ana Paula Rodrigues da Silva ◽  
Giseudo Aparecido Paiva ◽  
Ostenildo Ribeiro Campos ◽  
Adriana Matheus da Costa Figueiredo
Keyword(s):  
Plukenetia Volubilis ◽  
Sacha Inchi

A agricultura sustentável busca meios alternativos para controlar o ataque fúngico de acordo com as particularidades de cada fungo, com o intuito de reduzir o uso de agroquí­micos. Os extratos alcóolicos são uma alternativa, visto que são produtos naturais extraí­dos de plantas ou de partes da mesma que apresentam efeito fungicida. Desse modo o objetivo desse trabalho foi testar o controle in vitro do Rhizoctonia sp. utilizando diferentes concentrações de extrato alcoólicos de Sacha Inchi (Plukenetia volubilis). Para isso foi instalado um experimento em delineamento inteiramente ao acaso com 6 concentrações de extrato alcoólico de Sacha Inchi (0%, 2%, 4%, 6%, 8% e 10%) com 8 repetições. Foram mensurados o crescimento diário da colônia, o í­ndice de velocidade de crescimento micelial (IVCM) e a porcentagem de inibição de crescimento (PIC). Os dados obtidos foram analisados via analise de regressão polinomial utilizando o software R Studio. A partir da concentração de 6% o fungo apresenta pequeno desenvolvimento, e na dose máxima testada (10%) o crescimento do fungo foi praticamente nulo. O extrato alcoólico de Sacha Inchi foi eficiente a partir da concentração de 6%, o que o torna indicado para o controle do fungo Rhizoctonia sp

scholarly journals Micropropagation of White Flowering Eastern Redbud (Cercis canadensis var. alba L.)

1990 ◽  
Vol 8 (4) ◽  
pp. 177-179
Author(s):  
S. Yusnita ◽  
R. L. Geneve ◽  
S. T. Kester
Keyword(s):  
Root System ◽  
Day Treatment ◽  
Shoot Multiplication ◽  
Naphthaleneacetic Acid ◽  
Indolebutyric Acid ◽  
Cercis Canadensis ◽  
Half Strength ◽  
Eastern Redbud ◽  
Being Moved

Abstract A white flowering Eastern redbud (Cercis canadensis var. alba L.) has been successfully micropropagated. Two node explants collected from the initial flush of spring growth were cultured on woody plant medium (WPM). Increased shoot multiplication occurred at 10,15 and 20 μM (2.3, 3.4 and 4.5 ppm) benzyladenine (BA). Microshoots were rooted in vitro on half strength WPM with a 15-day treatment of 100 and 300 μM (18.6 and 55.9 ppm) α-naphthaleneacetic acid (NAA) or 100 and 300 μM (20.3 and 60.9 ppm) indolebutyric acid (IBA) prior to being moved to full strength WPM without growth regulators. Percentage rooting and the mean number of roots per cutting were comparable between NAA and IBA treated microcuttings, however, the subsequent root morphology differed between the two treatments. NAA treated plants developed a coarse, unbranched root system, while IBA treated cuttings developed a more desireable fine, branched root system. Rooted microshoots were successfully acclimated to greenhouse conditions.

In vitro propagation of Crambe maritima

1987 ◽  
Vol 65 (1) ◽  
pp. 72-75 ◽  
Author(s):  
J. Y. Peron ◽  
E. Regnier
Keyword(s):  
In Vitro Propagation ◽  
Indoleacetic Acid ◽  
Adventitious Shoots ◽  
Basal Medium ◽  
Naphthaleneacetic Acid ◽  
Petiole Explants ◽  
Acclimatization Period

A method for rapid micropropagation of sea kale (Crambe maritima L.) was developed. Petiole explants placed in vitro on a medium containing 0.5 mg/L indoleacetic acid (IAA), 6.0 mg/L kinetin, and 1.5 mg/L benzylaminopurine developed callus within 15 days and shoots within 28 days. Nearly four adventitious shoots could be developed within 3 weeks by placing the initial shoot on media without IAA. To develop roots, the shoots were then transferred to the basal medium containing 0.1 to 1.0 mg/L indolbutyric or α-naphthaleneacetic acid. Rooted plantlets were obtained within 2 or 3 weeks. After an acclimatization period of 6 weeks in a greenhouse in unsterilized medium, the plantlets could be set outdoors.

scholarly journals Micropropagation of Inula germanica L. from the Seedlings Explants

2018 ◽  
Vol 46 (1) ◽  
pp. 52-57 ◽  
Author(s):  
Alina TREJGELL ◽  
Monika KAMIŃSKA ◽  
Karolina LISOWSKA ◽  
Andrzej TRETYN
Keyword(s):  
Solid Medium ◽  
Positive Impact ◽  
Shoot Tips ◽  
Fluorescent Light ◽  
First Year ◽  
Naphthaleneacetic Acid ◽  
Multiplication Rate ◽  
Indolebutyric Acid ◽  
Regenerated Plants

This is the first communication of micropropagation system for Inula germanica using seedling explants germinated in vitro. The development of this system gives the possibility of future reintroduction of I. germanica providing a way to stabilize or re-establish its population. Shoot tips and fragments of cotyledons, hypocotyls and roots were isolated from ten-day-old seedlings. Explants were put on MS medium containing 1.0 mg l-1 benzylaminopurine and 0.1 mg l-1 naphthaleneacetic acid and cultured under continuous white fluorescent light (45 μmol.m-2.s-1) at 26 ± 1 °C. The highest percentage of shoot organogenesis (83.3%) was recorded for hypocotyl, while the highest average number of shoots per explant (12.0) was recorded for shoot tips. In subsequent subcultures, multiplication rate decreased to 3.0-4.9 shoots per explant. Less than 19% shoots were able to root on the solid medium without auxins. The highest rooting efficiency (69.3%) was recorded for solid medium supplemented with indolebutyric acid, but growth of roots was inhibited. The percentage of rooted shoots (62.2%) and number of roots per shoot (2.4 per shoot) into the liquid medium were comparable to medium with 0.1 mg·l-1 indolebutyric acid. showing a positive impact on the process of acclimatization. The regenerated plants were able to flowering in the first year after acclimatization. Developed micropropagation system for I. germanica is efficient and can be a useful tool for the active protection of this species.

scholarly journals In Vitro Propagation of Agave americana by Indirect Organogenesis

HortScience ◽  
2017 ◽  
Vol 52 (7) ◽  
pp. 996-999 ◽  
Author(s):  
Carlos Alberto Lecona-Guzmán ◽  
Sheila Reyes-Zambrano ◽  
Felipe Alonso Barredo-Pool ◽  
Miguel Abud-Archila ◽  
Joaquín Adolfo Montes-Molina ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Shoot Proliferation ◽  
Ms Medium ◽  
Indolebutyric Acid ◽  
Indirect Organogenesis ◽  
Meristematic Tissue ◽  
Phenotypic Alteration ◽  
Sexual And Asexual Reproduction ◽  
Agave Americana

Factors such as slow growth, low rates of sexual and asexual reproduction, and viability of seeds among others limit the massive propagation of Agave americana L. by conventional methods. In this study, callus induction and shoot proliferation was determined in A. americana using Murashige and Skoog (MS) medium supplemented with dicholorophenoxyacetic acid (2,4-D) and 6-benzyl adenine (BA). Meristematic tissue was used as the explants, and were placed on MS medium supplemented with 30.0 g·L−1 sucrose with 0.11, 0.18, or 0.45 μm 2,4-D and 11.0, 22.0, 38.2, 44.0, 58.7, or 73.3 μm BA. Treatments were implemented according to factorial experimental design 3 × 6. After 1 month, the number of explants with callus was determined, whereas the numbers of shoots per explant were monitored after 4, 16, 20, and 36 weeks. The maximum percent of explants with callus was obtained with 0.11 μm 2,4-D and 58.7 and 73.3 μm BA, whereas the maximum numbers of shoots per explant (71) were obtained with 0.11 μm 2,4-D and 73.3 μm BA. The effect of different concentrations of indolebutyric acid (IBA) in the rooting of shoots was evaluated. There were no significant effects of IBA on the number of roots, root length, and axillary roots. Plantlets were acclimatized in the glasshouse and they did not show any phenotypic alteration. This is a highly efficient protocol for the in vitro propagation of A. americana via indirect organogenesis.

scholarly journals Caracterización químico nutricional de tres semillas de oleaginosas disponibles en la región Amazonas

2021 ◽  
Vol 2 (3) ◽  
pp. 24
Author(s):  
Katerin Goñas ◽  
Wilmer Bernal
Keyword(s):  
Ricinus Communis ◽  
Salvia Hispanica ◽  
Plukenetia Volubilis ◽  
Sacha Inchi

<p>El objetivo del presente trabajo fue la caracterización de tres semillas de oleaginosas de la región Amazonas. Los siguientes parámetros evaluados fueron: humedad (H), proteína cruda (nitrógeno total) (%PT), cenizas (CZA%), fibra cruda (FC), extracto etéreo (EE), extracto libre de nitrógeno (ELN). Además se determinó energía bruta (EB), fibra detergente acida (FDA), fibra detergente neutra (FDN%), porcentaje de calcio (Ca) y fosforo (P) y digestibilidad in vitro. Las semillas de oleaginosas fueron semilla de Sacha Inchi (Plukenetia volúbilis linneo ) semilla de Higuerilla (Ricinus communis) y semilla de Chía {salvia hispánica). La clasificación final de dichos insumos se realizó por la concentración de nutrientes en proteicos, energéticos y fibrosos. Concluyendo que en la región Amazonas las semillas de oleaginosas poseen un alto potencial nutricional para la alimentación animal. Sin embargo, es necesario la caracterización del perfil de aminoácidos y componentes anti nutricionales en los insumos identificados.</p>

scholarly journals In vitro germination and vegetative propagation through bud development of sacha inchi (Plukenetia volubilis L.)

2021 ◽  
Vol 27 (1) ◽  
Author(s):  
Ana María Henao Ramírez ◽  
Aura Inés Urrea Trujillo ◽  
Lucía Atehortúa Garcés
Keyword(s):  
Seed Germination ◽  
Seed Coat ◽  
Vegetative Propagation ◽  
Plant Production ◽  
Nodal Segments ◽  
Oilseed Crop ◽  
Bud Development ◽  
Plukenetia Volubilis ◽  
Sacha Inchi

This study describes the in vitro seed germination and micropropagation of Plukenetia volubilis (sacha inchi), an oilseed crop rich in omega-3 fatty acids, with health benefits and several industrial applications. Seed germination was evaluated in different culture media (MS and 1/2 MS), seed coat presence/absence and culture temperature (18 °C and 28 °C). Micropropagation was performed using axillary bud development (ABD) on nodal segments from in vitro seedlings. KIN, BAP and 2-ip were evaluated for ABD, and the effect of modified MS in 453 mg L-1 CaCl2 and 351.62 mg L-1 MgSO4 on ABD and shoot survival was assessed to improve the process. Finally, six treatments were evaluated to optimize ABD and shoot leaf formation. Seed germination of 91.6 % was achieved in MS at 28 °C when the seed coat was removed. ABD was obtained in 45 % and 40 % with 0.4 mg L-1 KIN and 0.6 mg L-1 2-ip, respectively, with the least CAL. The modification in 453 mg L-1 CaCl2 then allowed 76 % ABD and 82 % explant survival. ABD response was optimized to 95 % and 2.45 leaves with MS medium + CaCl2 modification + 10 % coconut water + 0.4 mg L-1 KIN. The same results were obtained by replacing the latter with 0.6 mg L-1 2-ip. Rooting was achieved in MS without PGR, and acclimatization was successful. The results indicate that plant production via germination and vegetative propagation is effective for commercial purposes.

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