scholarly journals Valor nutritivo de la pulpa de café sometida a fermentación sólida usando Aspergillus niger en pollos y cerdos

2016 ◽  
Vol 1 ◽  
pp. 79 ◽  
Author(s):  
Mario Molina ◽  
Otto Raúl Lechuga ◽  
Ricardo Bressani
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Feed Efficiency ◽  
Nutritional Value ◽  
Fermentation Process ◽  
Initial Moisture Content ◽  
Original Material ◽  
Coffee Pulp ◽  
True Protein

Coffee pulp was subjected to a solid-state fermentation process using Aspergillus niger, an Initial moisture content of 80%, pH 3.5,35°C and adding 2.5% commercial urea and 2.0% commercial dicalcium phosphate, for a total of 48 h. The sundried fermented material proved to have a significantly (P<0.05) lower polyphenolic, caffeine and fiber content than the original sun-dried coffee pulp. Further, the true protein content of the fermented material (18%) was significantly (P<0.01) higher than that of the original material (5%). When the pulp was included at a 5, 10 and 15% levels In growing chicken rations It was found that after six weeks the ration containing 15% of the fermented pulp presented a weight gain (1.43 kg) and a feed efficiency (2.20) significantly equal to the control ration (without pulp) and better (P<0.05) than the ration containing the sundried, unfermented pulp at 15 % (1.19 kg and 2.55, respectively). In the case of growing swine fed with rations containing either fermented or unfermented pulp at 20% level for 8 weeks the results were similar to those obtained In the chicken experiment. It is concluded that solid-state fermentation represents a viable technological alternative to improve the nutritional value of coffee pulp.

Production and Characterization of Amylases by Aspergillus niger under Solid State Fermentation Using Agro Industrials Products

2006 ◽  
Vol 2 (3) ◽  
Author(s):  
Michele Rigon Spier ◽  
Adenise Lorenci Woiciechowski ◽  
Luciana Porto de Souza Vandenberghe ◽  
Carlos Ricardo Soccol
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Sugar Cane ◽  
Maximum Yield ◽  
Initial Moisture Content ◽  
Cassava Starch ◽  
Sugar Cane Bagasse ◽  
Production Costs

The use of agro industrials products cassava starch and sugar cane bagasse present a great potential as substrate and support, respectively, showing low production costs for amylases production under solid-state fermentation by Aspergillus niger LPB 28. The effect of various factors was examined and some variables were optimized to ?-amylase and amyloglucosidase production. Inoculum rate in different concentrations (105, 106, 107 and 108 spores/g of cassava starch) were used to inoculate the solid substrate for the SSF. The material was supplemented with nitrogen sources; calcium source and micronutrients solution. The ratios of cassava starch to sugar cane bagasse (1/1; 1.5/1; 1/1.5; 1/2; 2/1; 1/3; 3/1) were tested and the initial moisture content varying 80, 85 and 90%. Cultivation was carried out at temperatures 25, 30 and 35ºC for 48, 60 and 72 h. Study of influence of pH initial in SSF was conduced in pH 4.0, 5.0, 6.0, 7.0 and 8.0. Statistical analysis was carried out using the software STATISTICA 5.1 (StatSoft, Tulsa, OK, USA). A 32 complete factorial design (2 factors, 3 levels and 9 experiments) was used to study the effect of the factors in ?-amylase and amyloglucosidase production. The results showed cassava starch and sugar cane bagasse at the ratios of 2/1 was the best for optimum production of ?-amylase and amyloglucosidase. The maximum yield (?-amylase 1732.95 U/g of cassava starch and amyloglucosidase 2044.94 U/g of cassava starch) was achieved with optimized process parameters such as incubation period (60 hours), moisture level was 90%, inoculation rate (105 spores/g of dried material), pH 4.0 and fermentation temperature (30ºC). The nitrogen source (KNO3) also enhanced the ?-amylase and amyloglucosidase activities. In kinetic characterization of enzymes the Michaelis-Menten constant KM and maximum velocity Vmax (KM 10.84 g/l, Vmax 3.42 g/l) for ?-amylase and KM 11.32 g/l and Vmax 5.18 g/l for amyloglucosidase.

scholarly journals Production, Purification and Standardization of Anticancerous Enzyme (L-Asparaginases) from Aspergillus Niger Using Soil Samples by Solid State Fermentation

2014 ◽  
Vol 2 (4) ◽  
pp. 488-492 ◽  
Author(s):  
OM Fasalu Rahiman ◽  
Musambil Mohthash ◽  
U Salmanul Faris ◽  
TK Mohammed Muneersha ◽  
M Shejina
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Enzyme Production ◽  
Fermentation Process ◽  
Lymphoblastic Leukemia ◽  
The Other ◽  
Ammonium Ion ◽  
Bacterial Cells ◽  
Reliable Technique

Biotechnology techniques can provide an unlimited and pure source of enzymes as an alternative to the harsh chemicals traditionally used in industry for accelerating chemical reactions. L-asparaginase is one among them, found in various plants, animals and bacterial cells. Lasparaginase is studied to be responsible for catalyzing the deamination of Asparagines to yield Aspartic acid and an ammonium ion, resulting in depletion of free circulatory Asparagines in plasma. Its use in therapeutics is found to be remarkable, especially for those specific cases where blood cells become cancerous, such as in acute lymphoblastic leukemia. In this study we have made an effort to isolate, identify and screen micro-organism (Aspergillus niger) for the production of anticancerous enzyme (L-asparaginases) by solid state fermentation process and the produced enzyme was purified and characterized for L-asparaginases. The aim of the study was to validate the solid fermentation process in terms of its reliability and feasibility for production of L-asparaginase enzyme. This method was found to be very cost effective and reliable when compared to the other expensive techniques used around the globe for enzyme production. Even though the product yield and purity is comparatively less in comparison with the other techniques it can be still used as a reliable technique for short scale enzyme production. DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11278  Int J Appl Sci Biotechnol, Vol. 2(4): 488-492 

Pectinase-hyperproducing mutants of Aspergillus niger C28B25 for solid-state fermentation of coffee pulp

1993 ◽  
Vol 15 (3) ◽  
pp. 254-260 ◽  
Author(s):  
Philip Antier ◽  
Alfredo Minjares ◽  
Sevastianos Roussos ◽  
Maurice Raimbault ◽  
Gustavo Viniegra-Gonzalez
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Coffee Pulp

scholarly journals Activity of an A-L-Rhamnosidase Produced by Aspergillus niger During Solid State Fermentation of Coffee Pulp Wastes

2019 ◽  
Vol 4 (1) ◽  
pp. 105-111
Author(s):  
Kahar Muzakhar ◽  
Rudju Winarsa
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Maximum Activity ◽  
Reducing Sugar ◽  
Sugar Production ◽  
Coffee Pulp ◽  
Alkali Extract ◽  
Ph Range ◽  
Hydrolysis Activity

 An α-L-Rhamnosidase released by Aspergillus niger during solid-state fermentation (SSF) using coffee pulp (CP) wastes media has been investigated. The activity of α-L-Rhamnosidase based on reducing sugar production against 2% CP alkali extract substrate in 50 mM acetate buffer pH 5. The maximum activity of α-L-Rham-nosidase was obtained in sixth-day SSF with reducing sugar pro-duction of 13 μg/mL. The enzyme is actively hydrolyzed 0.1% p-ni-trophenyl-α-L-rhamnopyranoside (PNP-Rha) to 95% from initial concentration. Purification using DEAE-Toyopearl 650M increased hydrolysis activity ten times against the substrate, reaching 134 μg/mL of reducing sugar. Optimum enzyme activity at pH 4.5 and 50°C, while stable at pH and temperature in a pH range of 3.5-7 and below 50°C. 

scholarly journals EFFECT OF SOLID STATE FERMENTATION ON NUTRITIONAL CONTENT AND EVALUATION OF DEGRADABILITY IN CACTUS PEAR

2015 ◽  
Vol 28 (3) ◽  
pp. 248-254 ◽  
Author(s):  
TAMIRES CARVALHO DO SANTOS ◽  
GLEIZA ALVES DINIZ ◽  
AILA RIANY DE BRITO ◽  
AURELIANO JOSÉ VIEIRA PIRES ◽  
MARCELO FRANCO
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Protein Content ◽  
Solid State Fermentation ◽  
Nutritional Value ◽  
Animal Feed ◽  
State University ◽  
Protein Enrichment ◽  
Nutritional Content ◽  
Cactus Pear

ABSTRACT: The process of protein enrichment of cactus pear (Nopalea cochenillifera (L.) Salm Dyck by solid state fermentation with the use of Aspergillus niger and Rhyzopus sp. was studied for improving the nutritional value of this cactus species for use as animal feed. The experiments were conducted in the Agro-industrial Waste Laboratory of State University of Southwest Bahia (Brazil). To this end, we have evaluated the effects of biotransformation on the levels of protein, cellulose, hemicellulose, and lignin, as well as the potential degradability. Bioconversion was carried out using cactus pear as the only substrate, without supplementation with nitrogen, mineral and vitamin sources. The fermentation with Aspergillus niger promoted a 78% increase in/of protein content and reductions of cellulose, hemicellulose, and lignin of 40%, 36%, and 28%, respectively. Degradability, in turn, was observed to have increased by 66 % after 240 h. On the other hand, the fermentation with Rhyzopus sp. was less efficient, with a 69% increase in protein content, and reductions in cellulose, hemicellulose, and lignin contents of 30%, 28%, and 18%. In turn, degradability was seen to have increased by 51%. The fermentation of cactus pear by Aspergillus niger and Rhyzopus sp. exhibited the protein enrichment and increased protein degradability of this Cactaceae. Moreover, this is the most ever efficient micro-organism used in bioconversion. Based on the results, bioconversion of cactus is an excellent alternative to ruminant feeding in arid or semi-arid land.

scholarly journals Optimization of Protease from Aspergillus Niger under Solid-State Fermentation Utilizing Shrimp Shell Substrate

2021 ◽  
Vol 11 (6) ◽  
pp. 14809-14824
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Incubation Temperature ◽  
Proteolytic Enzymes ◽  
Initial Moisture Content ◽  
Inoculum Size ◽  
Protease Production ◽  
Shrimp Shell

Proteases, also known as proteinases or proteolytic enzymes, belong to a group of hydrolases. It can be applied in numerous fields and industries. Solid-state fermentation (SSF) is recognized as an effective method to produce protease. The ultimate aim of this study is to optimize the production of protease from Aspergillus niger under solid-state fermentation (SSF) by utilizing shrimp shell powder as a solid substrate. It was found that the produced protease from SSF was slightly alkaline. The correlation between factors operating parameters (incubation temperature, inoculum size, moisture content) for enzyme production is analyzed using statistical software, Minitab 16. A 23 full factorial experimental design was employed, and the enzyme produced was optimized by the method of desirability function. The optimal conditions for protease production of 3.7 U/mg were 35 °C of incubation temperature, 60% of initial moisture content, and 1.0 inoculum size. It is concluded that SSF protease was successfully produced from Aspergillus niger by utilizing shrimp waste as substrate. Through optimization study, moisture content, the interaction between incubation temperature and moisture content, interaction between moisture content and inoculum size significantly impact protease production.

scholarly journals Food Waste-Assisted Metal Extraction from Printed Circuit Boards: The Aspergillus niger Route

2021 ◽  
Vol 9 (5) ◽  
pp. 895
Author(s):  
Carlotta Alias ◽  
Daniela Bulgari ◽  
Fabjola Bilo ◽  
Laura Borgese ◽  
Alessandra Gianoncelli ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Food Waste ◽  
Solid State Fermentation ◽  
Printed Circuit Boards ◽  
Electronic Waste ◽  
Metal Extraction ◽  
Circuit Boards ◽  
Printed Circuit ◽  
Waste Printed Circuit Boards

A low-energy paradigm was adopted for sustainable, affordable, and effective urban waste valorization. Here a new, eco-designed, solid-state fermentation process is presented to obtain some useful bio-products by recycling of different wastes. Urban food waste and scraps from trimmings were used as a substrate for the production of citric acid (CA) by solid state fermentation of Aspergillus niger NRRL 334, with a yield of 20.50 mg of CA per gram of substrate. The acid solution was used to extract metals from waste printed circuit boards (WPCBs), one of the most common electronic waste. The leaching activity of the biological solution is comparable to a commercial CA one. Sn and Fe were the most leached metals (404.09 and 67.99 mg/L, respectively), followed by Ni and Zn (4.55 and 1.92 mg/L) without any pre-treatments as usually performed. Commercial CA extracted Fe more efficiently than the organic one (123.46 vs. 67.99 mg/L); vice versa, biological organic CA recovered Ni better than commercial CA (4.55 vs. 1.54 mg/L). This is the first approach that allows the extraction of metals from WPCBs through CA produced by A. niger directly grown on waste material without any sugar supplement. This “green” process could be an alternative for the recovery of valuable metals such as Fe, Pb, and Ni from electronic waste.

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