scholarly journals Phylogeny of Dengue virus type 2 isolated in the Central Highlands, Vietnam

2017 ◽  
Vol 65 (2) ◽  
Author(s):  
Tuan Van Le ◽  
Nguyen Thi Tuyet Van ◽  
Nguyen Hoang Quan ◽  
Pham Tho Duoc
Keyword(s):  
Phylogenetic Analysis ◽  
Dengue Virus ◽  
Dengue Fever ◽  
Virus Type ◽  
Public Health Issue ◽  
E Gene ◽  
Study Results ◽  
Central Highlands ◽  
Dengue Virus Type 2

Dengue fever is perhaps the most important viral re-emergent disease especially in tropical and sub-tropical countries, affecting about 50 million people around the world every year. In the Central Highlands regions of Vietnam, dengue fever still remains as a major public health issue. Although four viral serotypes have been currently identified, dengue virus type 2 (DENV-2) was involved in the most important outbreaks during 2010-2012, especially, 2010 when the fatality rate highly increased. Detection of genotype of DENV-2 provided information on origin, distribution and genotype of the virus. In this study, DEN-2 isolated from dengue patients during the 2010-2012 epidemics was amplified and sequenced with E gene. The consensus sequences were aligned with reference E gene sequences of globally available Genbank. Phylogenetic analysis was performed using Neighbor-joining and Kimura 2-parameter model to construct phylogenetic tree. A total of 15 isolates (seven from 2010; one from 2011 and seven from 2012) were obtained from human serum samples. Phylogenetic analysis revealed that Asian genotype 1 is currently circulating locally in Central Highlands region. Isolates of this genotype were closely related to viruses from Thailand, Laos, and Cambodia. It indicated that these epidemics maybe imported into the Central Highlands region from South-East Asia neighbor countries. The study results would help in planning for prevention and control of dengue virus in Vietnam. Continuous monitoring of DENV genotypes is necessary to confirm the current findings and detect possible genotype shifts within the dengue viruses in the future.

Dengue virus type 2 in Cuba, 1997: conservation of E gene sequence in isolates obtained at different times during the epidemic

2004 ◽  
Vol 150 (3) ◽  
pp. 415-425 ◽  
Author(s):  
R. Rodriguez-Roche ◽  
M. Alvarez ◽  
T. Gritsun ◽  
D. Rosario ◽  
S. Halstead ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Gene Sequence ◽  
E Gene ◽  
Dengue Virus Type 2

scholarly journals Complete Coding Sequences of Dengue Virus Type 2 Strains from Febrile Patients Seen in Malindi District Hospital, Kenya, during the 2017 Dengue Fever Outbreak

2018 ◽  
Vol 6 (15) ◽  
pp. e00076-18 ◽  
Author(s):  
Kimita Gathii ◽  
Josphat N. Nyataya ◽  
Beth K. Mutai ◽  
George Awinda ◽  
John N. Waitumbi
Keyword(s):  
Dengue Virus ◽  
Dengue Fever ◽  
Virus Type ◽  
District Hospital ◽  
Coding Sequences ◽  
Malindi District ◽  
Dengue Virus Type 2

ABSTRACT We report here 10 complete polyprotein-coding sequences of dengue virus type 2 strains isolated from febrile patients who presented at Malindi District Hospital, Kenya, during a recent dengue fever outbreak. Phylogenetically, all the strains belonged to clonal serotype 2 of the Cosmopolitan genotype.

scholarly journals Complete Genome Sequence of Dengue virus Type 2 from a Resident of North-Central Florida with Locally Transmitted Dengue Fever

2017 ◽  
Vol 5 (31) ◽  
Author(s):  
Sarah K. White ◽  
Nicole M. Iovine ◽  
L. Connor Nickels ◽  
J. Glenn Morris ◽  
John A. Lednicky
Keyword(s):  
United States ◽  
Dengue Virus ◽  
Dengue Fever ◽  
Virus Type ◽  
The United States ◽  
North Central ◽  
Central Florida ◽  
Autochthonous Transmission ◽  
Dengue Virus Type 2

ABSTRACT The majority of dengue fever cases reported in the United States recently have been imported. We isolated dengue virus type 2 (DENV-2) from a North-Central Florida resident with locally acquired dengue fever in May 2016. This is the first evidence of autochthonous transmission of the virus in north-central Florida.

scholarly journals An outbreak of dengue virus (DENV) type 2 Cosmopolitan genotype in Israeli travellers returning from the Seychelles, April 2017

2017 ◽  
Vol 22 (26) ◽  
Author(s):  
Yaniv Lustig ◽  
Dana Wolf ◽  
Ora Halutz ◽  
Eli Schwartz
Keyword(s):  
Phylogenetic Analysis ◽  
Virus Infection ◽  
Dengue Virus ◽  
Virus Type ◽  
Dengue Virus Infection ◽  
Diagnostic Capabilities ◽  
Dengue Virus Type 2

Dengue virus infection was diagnosed in six Israeli travellers returning from the Seychelles in April 2017. Phylogenetic analysis identified identical sequences belonging to the Cosmopolitan genotype of dengue virus type 2 in all samples sequenced, thus providing evidence for a probable dengue type 2 outbreak in the Seychelles. This report further demonstrates the role of travellers as sentinels for arboviral infections, especially in countries with limited diagnostic capabilities.

scholarly journals Dengue type 2 outbreak in the south of the State of Bahia, Brazil: laboratorial and epidemiological studies

1995 ◽  
Vol 37 (6) ◽  
pp. 507-510 ◽  
Author(s):  
Rita M. R. Nogueira ◽  
Marize P. Miagostovich ◽  
Hermann G. Schatzmayr ◽  
Gerusa C. Moraes ◽  
Floriene M. A. Cardoso ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Dengue Fever ◽  
Virus Type ◽  
Signs And Symptoms ◽  
Epidemiological Studies ◽  
Acute Disease ◽  
Santa Cruz ◽  
Virus Activity ◽  
Dengue Virus Type 2

During March 1994 cases of a exanthematic acute disease were reported in the municipalities of Itagemirim, Eunápolis and Belmonte, state of Bahia. Dengue fever was confirmed by serology (MAC-ELISA) and by dengue virus type 2 isolation, genotype Jamaica. Signs and symptoms of classic dengue fever were observed with a high percentual of rash (73.8%) and pruritus (50.5%). Major haemorrhagic manifestations were unfrequent and only bleeding gum was reported. Dengue virus activity spreaded rapidly to important tourism counties like Porto Seguro, Ilhéus, Santa Cruz de Cabrália, Prado, Alcobaça and others, representing a risk for the spreading of dengue virus into the country and abroad.

scholarly journals Reduced sphingosine kinase 1 activity in dengue virus type-2 infected cells can be mediated by the 3′ untranslated region of dengue virus type-2 RNA

2013 ◽  
Vol 94 (11) ◽  
pp. 2437-2448 ◽  
Author(s):  
J. M. Carr ◽  
T. Kua ◽  
J. N. Clarke ◽  
J. K Calvert ◽  
J. R. Zebol ◽  
...  
Keyword(s):  
Cell Death ◽  
Dengue Virus ◽  
Virus Type ◽  
Elongation Factor ◽  
Sphingosine Kinase ◽  
Sphingosine Kinase 1 ◽  
Diarrhea Virus ◽  
Infected Cells ◽  
Dengue Virus Type 2

Sphingosine kinase 1 (SphK1) is a lipid kinase with important roles including regulation of cell survival. We have previously shown reduced SphK1 activity in cells with an established dengue virus type-2 (DENV-2) infection. In this study, we examined the effect of alterations in SphK1 activity on DENV-2 replication and cell death and determined the mechanisms of the reduction in SphK1 activity. Chemical inhibition or overexpression of SphK1 after established DENV-2 infection had no effect on infectious DENV-2 production, although inhibition of SphK1 resulted in enhanced DENV-2-induced cell death. Reduced SphK1 activity was observed in multiple cell types, regardless of the ability of DENV-2 infection to be cytopathic, and was mediated by a post-translational mechanism. Unlike bovine viral diarrhea virus, where SphK1 activity is decreased by the NS3 protein, SphK1 activity was not affected by DENV-2 NS3 but, instead, was reduced by expression of the terminal 396 bases of the 3′ UTR of DENV-2 RNA. We have previously shown that eukaryotic elongation factor 1A (eEF1A) is a direct activator of SphK1 and here DENV-2 RNA co-localized and co-precipitated with eEF1A from infected cells. We propose that the reduction in SphK1 activity late in DENV-2-infected cells is a consequence of DENV-2 out-competing SphK1 for eEF1A binding and hijacking cellular eEF1A for its own replication strategy, rather than a specific host or virus-induced change in SphK1 to modulate viral replication. Nonetheless, reduced SphK1 activity may have important consequences for survival or death of the infected cell.

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