Relationship between biomarkers of muscle damage and redox status in response to a weightlifting training session: effect of time-of-day

Objective Purpose：Downhill running can causes muscle damage, called delayed muscle damage and induced oxidative stress and inflammatory reaction, causing abnormity of skeletal muscle morphology, changing in blood biochemical indexes, and decreasing in function of skeletal muscle systolic. Asymmetric dimethylarginine (ADMA), an endogenous nitric oxide synthase (NOS) inhibitor, is degraded by dimethylarginine dimethylaminohydrolase 1 (DDAH1). There were new evidences demonstrated that DDAH1 is an important regulator of cell redox state and apoptosis. In summary, the study shown that DDAH1 is an important regulator of cell redox state and apoptosis. Emerging evidences suggests that DDAH1 controls cellular oxidative stress and apoptosis via a miR-21-dependent pathway. However, the effect and mechanism of DDAH1 on damage of skeletal muscle caused by downhill running is not clear enough. Thus，the purpose of this experiment was to determine the effect and mechanism of DDAH1 in downhill running. Keys: downhill running; delayed onset muscle soreness(DOMS); eccentric exercise; skeletal muscle. Methods Method: The experimental mice were 24 female C57 mice of 10 weeks old and 24 female DDAH1 hybrid knockout mice of 10 weeks old. DDAH1 KO mice used for this study was knockout of dimethylarginine dimethylaminohydrolase 1 compared with WT mice. Animals were fed standard laboratory chow and had access to water ad libitum. C57 mice were divided into 3 groups: C57 control, C57 48H, C57 120H; DDAH1 KO mice were divided into 3 groups: DDAH1 control, DDAH1 48H, DDAH1 120H. C57 and DDAH1 KO mice used for this study completed a single bout of downhill running exercise (20°, 17 m/min, 60 min), and gastrocnemius muscle, soleus muscle and quadriceps femoris muscle were collected 48 and 120 hours (H) postexercise (PE). C57control group and DDAH1 KO control group dose not exercise. Speed on the treadmill was gradually increased from 10 to 17m/min during a 7-min warm-up period (increased of 1m/min every minute). All experiments were conducted at approximately the same time of day. Maximal grip strength was measured ifor each groups. Grip strength testing was completed to detect post-eccentric exercise injury in C57 and DDAH1 KO mice. All results were analyzed by means of methods of histological and molecular biological. Results Method: The experimental mice were 24 female C57 mice of 10 weeks old and 24 female DDAH1 hybrid knockout mice of 10 weeks old. DDAH1 KO mice used for this study was knockout of dimethylarginine dimethylaminohydrolase 1 compared with WT mice. Animals were fed standard laboratory chow and had access to water ad libitum. C57 mice were divided into 3 groups: C57 control, C57 48H, C57 120H; DDAH1 KO mice were divided into 3 groups: DDAH1 control, DDAH1 48H, DDAH1 120H. C57 and DDAH1 KO mice used for this study completed a single bout of downhill running exercise (20°, 17 m/min, 60 min), and gastrocnemius muscle, soleus muscle and quadriceps femoris muscle were collected 48 and 120 hours (H) postexercise (PE). C57control group and DDAH1 KO control group dose not exercise. Speed on the treadmill was gradually increased from 10 to 17m/min during a 7-min warm-up period (increased of 1m/min every minute). All experiments were conducted at approximately the same time of day. Maximal grip strength was measured ifor each groups. Grip strength testing was completed to detect post-eccentric exercise injury in C57 and DDAH1 KO mice. All results were analyzed by means of methods of histological and molecular biological. Conclusions Conclusion: The DDAH1 knockout has a protective effect on delayed onset muscle soreness(DOMS) caused by downhill running, and accelerate the injury recovery.

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Effects of time-of-day on oxidative stress, cardiovascular parameters, biochemical markers, and hormonal response following level-1 Yo-Yo intermittent recovery test

Physiology International ◽

10.1556/2060.104.2017.1.6 ◽

2017 ◽

Vol 104 (1) ◽

pp. 77-90 ◽

Cited By ~ 3

Author(s):

K Aloui ◽

S Abedelmalek ◽

H Chtourou ◽

DP Wong ◽

N Boussetta ◽

...

Keyword(s):

Oxidative Stress ◽

Blood Pressure ◽

Muscle Damage ◽

Biochemical Markers ◽

Density Lipoprotein ◽

Time Of Day ◽

Hormonal Response ◽

Cardiovascular Parameters ◽

Recovery Test ◽

Level 1

The aim of this study was to investigate the effect of time-of-day on oxidative stress, cardiovascular parameters, muscle damage parameters, and hormonal responses following the level-1 Yo-Yo intermittent recovery test (YYIRT). A total of 11 healthy subjects performed an intermittent test (YYIRT) at two times-of-day (i.e., 07:00 h and 17:00 h), with a recovery period of ≥36 h in-between, in a randomized order. Blood samples were taken at the rest (baseline) and immediately (post-YYIRT) after the YYIRT for measuring oxidative stress, biochemical markers, and hormonal response. Data were statistically analyzed using one-way and two-way repeated measures ANOVA and Bonferroni test at p < 0.05. Observed power (α = 0.05) and partial eta-squared were used. Our results showed that oxygen uptake (VO2max), maximal aerobic speed, and the total distance covered tended to be higher in the evening (17:00 h). There was also a main effect of time-of-day for cortisol and testosterone concentration, which were higher after the YYIRT in the morning (p < 0.05). The heart rate peak and the rating of perceived exertion scales were lower in the morning (p < 0.05). However, the plasma glucose (p < 0.01), malondialdehyde, creatine kinase (p < 0.01), lactate dehydrogenase (p < 0.05), high-density lipoprotein (p < 0.01), total cholesterol (p < 0.01), and triglycerides (p < 0.05) were higher after the YYIRT in the evening. Low-density lipoprotein, systolic blood pressure, diastolic blood pressure, and lactate levels (p > 0.05) were similar for the morning and evening test. In conclusion, our findings suggest that aerobic performance presents diurnal variation with great result observed in the evening accompanied by an improvement of hormonal, metabolic, and oxidative responses. These data may help to guide athletes and coaches and contribute to public health recommendations on exercise and muscle damage particularly in the competitive periods.

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The Effects of Resistance Exercise on Muscle Damage, Position Sense, and Blood Redox Status in Young and Elderly Individuals

Geriatrics ◽

10.3390/geriatrics2030020 ◽

2017 ◽

Vol 2 (3) ◽

pp. 20 ◽

Cited By ~ 3

Author(s):

Michalis Nikolaidis

Keyword(s):

Resistance Exercise ◽

Muscle Damage ◽

Redox Status ◽

Position Sense ◽

Elderly Individuals

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Effects of a Strength Training Session After an Exercise Inducing Muscle Damage on Recovery Kinetics

The Journal of Strength and Conditioning Research ◽

10.1519/jsc.0000000000001479 ◽

2017 ◽

Vol 31 (1) ◽

pp. 115-125 ◽

Cited By ~ 7

Author(s):

Abd-Elbasset Abaïdia ◽

Barthélémy Delecroix ◽

Cédric Leduc ◽

Julien Lamblin ◽

Alan McCall ◽

...

Keyword(s):

Strength Training ◽

Muscle Damage ◽

Training Session

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Training session intensity affects plasma redox status in amateur rhythmic gymnasts

Journal of Sport and Health Science ◽

10.1016/j.jshs.2016.04.008 ◽

2019 ◽

Vol 8 (6) ◽

pp. 561-566 ◽

Cited By ~ 1

Author(s):

Marianna Bellafiore ◽

Antonino Bianco ◽

Giuseppe Battaglia ◽

Maria Silvia Naccari ◽

Giovanni Caramazza ◽

...

Keyword(s):

Training Session ◽

Redox Status ◽

Rhythmic Gymnasts

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Single resistance training session leads to muscle damage without isometric strength decrease

Journal of Human Sport and Exercise ◽

10.14198/jhse.2018.132.02 ◽

2018 ◽

Vol 13 (2) ◽

Cited By ~ 1

Author(s):

Gustavo Barquilha ◽

Jean Carlos Silvestre ◽

Yuri Lopes Motoyama ◽

Paulo Henrique Silva Marques de Azevedo

Keyword(s):

Resistance Training ◽

Muscle Damage ◽

Training Session ◽

Isometric Strength

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Effect of whey vs. soy protein supplementation on recovery kinetics following speed endurance training in competitive male soccer players: a randomized controlled trial

Journal of the International Society of Sports Nutrition ◽

10.1186/s12970-021-00420-w ◽

2021 ◽

Vol 18 (1) ◽

Author(s):

Savvas Kritikos ◽

Konstantinos Papanikolaou ◽

Dimitrios Draganidis ◽

Athanasios Poulios ◽

Kalliopi Georgakouli ◽

...

Keyword(s):

Endurance Training ◽

Muscle Damage ◽

Soy Protein ◽

Protein Intake ◽

Redox Status ◽

Protein Supplementation ◽

Soccer Players ◽

Protein Carbonyls ◽

Maximal Voluntary Isometric Contraction ◽

Exercise Induced

Abstract Background Soccer-specific speed-endurance training induces short-term neuromuscular fatigue and performance deterioration over a 72-h recovery period, associated with elevated markers of exercise-induced muscle damage. We compared the effects of whey vs. soy protein supplementation on field activity, performance, muscle damage and redox responses following speed-endurance training in soccer players. Methods Ten well-trained, male soccer players completed three speed-endurance training trials, receiving whey protein (WP), soy protein (SP) or an isoenergetic placebo (PL; maltodextrin) according to a randomized, double-blind, crossover, repeated-measures design. A pre-loading period was applied in each trial during which protein supplementation was individually adjusted to reach a total protein intake of 1.5 g/kg/day, whereas in PL protein intake was adjusted at 0.8–1 g/kg/day. Following pre-loading, two speed-endurance training sessions (1 and 2) were performed 1 day apart, over a 3-day experimental period. During each session, field activity and heart rate were continuously monitored using global positioning system and heart rate monitors, respectively. Performance (isokinetic strength of knee extensors and flexors, maximal voluntary isometric contraction, speed, repeated sprint ability, countermovement jump), muscle damage (delayed-onset of muscle soreness, creatine kinase activity) and redox status (glutathione, total antioxidant capacity, protein carbonyls) were evaluated at baseline (pre), following pre-loading (post-load), and during recovery from speed-endurance training. Results High-intensity and high-speed running decreased (P ≤ 0.05) during speed-endurance training in all trials, but WP and SP mitigated this response. Isokinetic strength, maximal voluntary isometric contraction, 30-m speed, repeated sprint ability and countermovement jump performance were similarly deteriorated during recovery following speed-endurance training in all trials (P ≤ 0.05). 10 m speed was impaired at 24 h only in PL. Delayed-onset of muscle soreness, creatine kinase, total antioxidant capacity and protein carbonyls increased and glutathione decreased equally among trials following speed-endurance training (P ≤ 0.05), with SP inducing a faster recovery of protein carbonyls only at 48 h (P ≤ 0.05) compared to WP and PL. Conclusions In conclusion, increasing daily protein intake to 1.5 g/kg through ingestion of either whey or soy protein supplements mitigates field performance deterioration during successive speed-endurance training sessions without affecting exercise-induced muscle damage and redox status markers. Trial registration Name of the registry: clinicaltrials.gov. Trial registration: NCT03753321. Date of registration: 12/10/2018.

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The effect of time of day and recovery type after a football game on muscle damage and performance in anaerobic tests on young soccer players

Biological Rhythm Research ◽

10.1080/09291016.2016.1197505 ◽

2016 ◽

Vol 47 (5) ◽

pp. 797-814 ◽

Cited By ~ 1

Author(s):

Intissar Shimi ◽

Salma Abedelmalek ◽

Khouloud Aloui ◽

Hamdi Chtourou ◽

Nizar Souissi

Keyword(s):

Muscle Damage ◽

Time Of Day ◽

Soccer Players ◽

Football Game ◽

And Performance

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The benefit of a supplement with the antioxidant melatonin on redox status and muscle damage in resistance-trained athletes

Applied Physiology Nutrition and Metabolism ◽

10.1139/apnm-2016-0677 ◽

2017 ◽

Vol 42 (7) ◽

pp. 700-707 ◽

Cited By ~ 16

Author(s):

Roberto C. Leonardo-Mendonça ◽

Javier Ocaña-Wilhelmi ◽

Tomás de Haro ◽

Carlos de Teresa-Galván ◽

Eduardo Guerra-Hernández ◽

...

Keyword(s):

Oxidative Stress ◽

Muscle Damage ◽

Antioxidant Properties ◽

Redox Status ◽

Absorption Capacity ◽

Double Blind ◽

Beneficial Effects ◽

Before And After ◽

High Doses ◽

Exercise Induced

Previous data showed that the administration of high doses of melatonin improved the circadian system in athletes. Here, we investigated in the same experimental paradigm whether the antioxidant properties of melatonin has also beneficial effects against exercise-induced oxidative stress and muscle damage in athletes. Twenty-four athletes were treated with 100 mg·day−1 of melatonin or placebo 30 min before bedtime during 4 weeks in a randomized double-blind scheme. Exercise intensity was higher during the study that before starting it. Blood samples were collected before and after treatment, and plasma was used for oxygen radical absorption capacity (ORAC), lipid peroxidation (LPO), nitrite plus nitrate (NOx), and advanced oxidation protein products (AOPP) determinations. Glutathione (GSH), glutathione disulphide (GSSG) levels, and glutathione peroxidase (GPx) and reductase (GRd) activities, were measured in erythrocytes. Melatonin intake increased ORAC, reduced LPO and NOx levels, and prevented the increase of AOPP, compared to placebo group. Melatonin was also more efficient than placebo in reducing GSSG·GSH−1 and GPx·GRd−1 ratios. Melatonin, but not placebo, reduced creatine kinase, lactate dehydrogenase, creatinine, and total cholesterol levels. Overall, the data reflect a beneficial effect of melatonin treatment in resistance-training athletes, preventing extra- and intracellular oxidative stress induced by exercise, and yielding further skeletal muscle protection against exercise-induced oxidative damage.

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