scholarly journals Identification of ovine-associated staphylococci by MALDI-TOF mass spectrometry

2021 ◽  
Author(s):  
A. Tonamo ◽  
I. Komlósi ◽  
L. Varga ◽  
M. Kačániová ◽  
F. Peles
Keyword(s):  
Mass Spectrometry ◽  
Raw Milk ◽  
Bacterial Isolates ◽  
Bulk Tank Milk ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Bulk Tank ◽  
Raw Milk Cheese ◽  
Tof Ms

AbstractThe objective of this study was to use matrix-assisted laser desorption ionisation–time of flight mass spectrometry (MALDI-TOF MS) for the identification of ovine-associated staphylococci. Presumptive Staphylococcus isolates were recovered from ovine udder surface (US), individual raw milk, bulk tank milk, and cheese samples and were characterised by conventional phenotypic methods. A total of 69 bacterial isolates were further confirmed by MALDI-TOF MS. Forty-two (60.9%) of 69 isolates were successfully identified on genus and species level. Two thirds (n = 28) of the 42 identified isolates were shown to be Staphylococcus spp. These 28 staphylococcal isolates formed two clusters, one consisting of 22 Staphylococcus aureus strains and the other composed of 6 non-aureus staphylococci, including S. simulans (n = 3), S. auricularis, S. equorum, and S. haemolyticus. MALDI-TOF MS has proven to be a reliable tool for the identification of staphylococci from raw sheep's milk, especially bulk tank milk; however, currently it appears to be less useful for the identification of bacterial isolates originating from ovine US samples. This is the first study to evaluate the applicability of MALDI-TOF MS for identification of Staphylococcus spp. in ovine raw milk, cheese, and US samples in Hungary.

scholarly journals Application of MALDI-TOF mass spectrometry in clinical diagnostic microbiology

2014 ◽  
Vol 8 (09) ◽  
pp. 1081-1088 ◽  
Author(s):  
Elena De Carolis ◽  
Antonietta Vella ◽  
Luisa Vaccaro ◽  
Riccardo Torelli ◽  
Teresa Spanu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drug Susceptibility ◽  
Biochemical Tests ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Bacteria And Fungi ◽  
Diagnostic Microbiology ◽  
Tof Ms

Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently emerged as a powerful technique for identification of microorganisms, changing the workflow of well-established laboratories so that its impact on microbiological diagnostics has been unparalleled. In comparison with conventional identification methods that rely on biochemical tests and require long incubation procedures, MALDI-TOF MS has the advantage of identifying bacteria and fungi directly from colonies grown on culture plates in a few minutes and with simple procedures. Numerous studies on different systems available demonstrate the reliability and accuracy of the method, and new frontiers have been explored besides microbial species level identification, such as direct identification of pathogens from positive blood cultures, subtyping, and drug susceptibility detection.

scholarly journals MALDI-TOF Mass Spectrometry and Specific Biomarkers: Potential New Key for Swift Identification of Antimicrobial Resistance in Foodborne Pathogens

2019 ◽  
Vol 7 (12) ◽  
pp. 593 ◽  
Author(s):  
Maureen Feucherolles ◽  
Henry-Michel Cauchie ◽  
Christian Penny
Keyword(s):  
Mass Spectrometry ◽  
Antimicrobial Resistance ◽  
Foodborne Pathogens ◽  
Reference Method ◽  
Multi Drug Resistance ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
The Future ◽  
Tof Ms

Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) is today the reference method for direct identification of microorganisms in diagnostic laboratories, as it is notably time- and cost-efficient. In the context of increasing cases of enteric diseases with emerging multi-drug resistance patterns, there is an urgent need to adopt an efficient workflow to characterize antimicrobial resistance (AMR). Current approaches, such as antibiograms, are time-consuming and directly impact the “patient-physician” workflow. Through this mini-review, we summarize how the detection of specific patterns by MALDI-TOF MS, as well as bioinformatics, become more and more essential in research, and how these approaches will help diagnostics in the future. Along the same lines, the idea to export more precise biomarker identification steps by MALDI-TOF(/TOF) MS data towards AMR identification pipelines is discussed. The study also critically points out that there is currently still a lack of research data and knowledge on different foodborne pathogens as well as several antibiotics families such as macrolides and quinolones, and many questions are still remaining. Finally, the innovative combination of whole-genome sequencing and MALDI-TOF MS could be soon the future for diagnosis of antimicrobial resistance in foodborne pathogens.

scholarly journals Matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS) in clinical mircobiological routine diagnostics1)

2011 ◽  
Vol 35 (4) ◽  
pp. ---
Author(s):  
Sören Schubert ◽  
Andreas Wieser
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tract Infections ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Abstract Very recently a novel method for differentiation of bacteria and fungi was developed, that is, identification by means of matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS). This differentiation relies on the exact measurement of species-specific protein spectra of ribosomal proteins. It is at least as accurate as conventional biochemical differentiation methods, but provides results within minutes. In addition to differentiation of bacteria and yeasts grown on agar plates, direct identification is feasible from positive blood cultures as well as from urine samples of patients suffering from urinary tract infections. Future developments of MALDI-TOF MS for clinical microbiological purposes include the detection of β-lactamase and carbapenemase activity as well as genotyping of bacteria below the species level.

scholarly journals High mass resolution MALDI-TOF MS for profiling biomolecules in mixtures

2018 ◽  
Author(s):  
Wenfa Ng
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
High Sensitivity ◽  
Mass Resolution ◽  
High Mass ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
High Mass Resolution ◽  
Tof Ms

Intact biomolecules carry its identity through its atomic constituents and mass, while fragmented biomolecules require reconstruction for their identity to be retrieved. Hence, for profiling biomolecules in mixtures, the goal would be the gentle ionization of biomolecules by mass spectrometry without inducing fragmentation. Doing so generates an ensemble of ionized intact biomolecules able to be profiled by high sensitivity time-of-flight detector for accurate determination of each biomolecule mass, and thus, identity. Specifically, in time-of-flight detection, high mass resolution determination would require high sensitivity in detecting small differences in time of arrival of biomolecule ions to the detector. While current time-of-flight mass spectrometry provides high mass resolution, greater mass resolution is needed for discriminating different biomolecules in a mixture, where mass differences between biomolecules could be at the sub-Dalton level. With the ability to reliably detect biomolecules with sub-Dalton mass resolution, mass spectrometry with time-of-flight detector such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) could find use in identifying the compendium of biomolecules present in a mixture without tedious and time-consuming separation. The larger question would subsequently be coupling sample preparation needs with the conditions conducive for MALDI-TOF MS analysis. Overall, high mass resolution mass spectrometry techniques for profiling biomolecules would find use as an enabling tool in many areas of analytical science and biological sciences such as proteomics and metabolomics.

scholarly journals Current Status of Matrix-Assisted Laser Desorption/Ionization–Time-of-Flight Mass Spectrometry (MALDI-TOF MS) in Clinical Diagnostic Microbiology

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4775
Author(s):  
Sachio Tsuchida ◽  
Hiroshi Umemura ◽  
Tomohiro Nakayama
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Clinical Applications ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms ◽  
Matrix Assisted Laser

Mass spectrometry (MS), a core technology for proteomics and metabolomics, is currently being developed for clinical applications. The identification of microorganisms in clinical samples using matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS) is a representative MS-based proteomics application that is relevant to daily clinical practice. This technology has the advantages of convenience, speed, and accuracy when compared with conventional biochemical methods. MALDI-TOF MS can shorten the time used for microbial identification by about 1 day in routine workflows. Sample preparation from microbial colonies has been improved, increasing the accuracy and speed of identification. MALDI-TOF MS is also used for testing blood, cerebrospinal fluid, and urine, because it can directly identify the microorganisms in these liquid samples without prior culture or subculture. Thus, MALDI-TOF MS has the potential to improve patient prognosis and decrease the length of hospitalization and is therefore currently considered an essential tool in clinical microbiology. Furthermore, MALDI-TOF MS is currently being combined with other technologies, such as flow cytometry, to expand the scope of clinical applications.

A brief identification of lactic acid bacteria isolated from Algerian goat’s Jben by MALDI-TOF MS

2018 ◽  
Vol 7 (4) ◽  
pp. 157-165
Author(s):  
Naïma L. Meghoufel ◽  
Abdelkader Homrani ◽  
Saïd Nemmiche ◽  
Nawel Benkrizi ◽  
Abdelkader E. Dahou ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Low Cost ◽  
Raw Milk ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Sub Saharan ◽  
Tof Ms

In this paper, we investigated the lactic acid bacteria’s community of the Algerian goat’s Jben in order to define and preserve it. This cheese variety is only handmade with raw milk, and a dried flower of Cynara cardunculus is used instead of the animal rennet, it is also restricted in sub-Saharan prov-inces in Algeria, and no previous studies on its lactic acid bacteria composi-tion have been performed before. Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) procedure was used to identify 36 lactic acid bacteria isolated from artisanal Jben made from Arabia goat’s raw milk in Naâma (Algeria). The results showed the domination of the Enterococcus genus presents with four species: E. durans, E. faecalis, E. hirae and E. faecium. Lactococcus lactis and Leuconostoc mesenteroïdes were also detected. The species identified were favoured by the composition of goat’s raw milk microflora used and the artisanal manufacturing process of the cheese. The use of MALDI-TOF MS method provided us with a rapid diagnosis of the Jben lactic acid bacteria’s community. This method based on microbial abundant proteins fingerprint diagnosis was chosen for its fast, precise, low cost and less complicated analyse.

Comparative evaluation of matrix-assisted laser desorption ionisation-time of flight mass spectrometry and conventional phenotypic-based methods for identification of clinically important yeasts in a UK-based medical microbiology laboratory

2015 ◽  
Vol 68 (12) ◽  
pp. 1040-1042 ◽  
Author(s):  
Nita Fatania ◽  
Mark Fraser ◽  
Mike Savage ◽  
Jason Hart ◽  
Alireza Abdolrasouli
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Microbiology Laboratory ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Phenotypic Methods ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

AimsPerformance of matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS) was compared in a side-by side-analysis with conventional phenotypic methods currently in use in our laboratory for identification of yeasts in a routine diagnostic setting.MethodsA diverse collection of 200 clinically important yeasts (19 species, five genera) were identified by both methods using standard protocols. Discordant or unreliable identifications were resolved by sequencing of the internal transcribed spacer region of the rRNA gene.ResultsMALDI-TOF and conventional methods were in agreement for 182 isolates (91%) with correct identification to species level. Eighteen discordant results (9%) were due to rarely encountered species, hence the difficulty in their identification using traditional phenotypic methods.ConclusionsMALDI-TOF MS enabled rapid, reliable and accurate identification of clinically important yeasts in a routine diagnostic microbiology laboratory. Isolates with rare, unusual or low probability identifications should be confirmed using robust molecular methods.

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