Egy különleges pancreaselváltozás: kevert differenciációjú nodularitás és diffúz szigetsejtes hyperplasia

Összefoglaló. A szerzők egy különleges pancreaselváltozás esetét ismertetik, melyben az acinusok neuroendokrin jellegű transzformációja diffúz, atípusos megjelenésű szigetsejtes hyperplasiával társult, valamint a pancreas mindhárom sejtvonalát (acinaris, ductalis, insularis) tartalmazó nodulusok képződtek. A komplex megjelenés ellenére a kórfolyamat nem járt endokrin tünetekkel. Esetünkben a kiváltó ok hátterében a struktúrák kóros progenitorsejt-differenciációja állhatott. Az irodalomban ilyen közlés eddig nem ismert. Orv Hetil. 2021; 162(6): 227–232. Summary. The authors present a case of a peculiar pancreatic lesion, in which the neuroendocrine transformation of the acini was associated with a diffuse, atypical insular hyperplasia, and micronodules exhibiting trilineage differentiation. Despite the complex alteration, no endocrine symptoms were noted. The case may represent the result of an abnormal pancreatic differentiation raising the possibility of reprogramming of the progenitor cells. To the best of our knowledge, this is the first report of such a lesion in the literature. Orv Hetil. 2021; 162(6): 227–232.

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Abstract 11: Xenogen-free In Vitro Differentiation and Expansion of Human Pluripotent Stem Cell-Derived Cardiovascular Progenitor Cells

Circulation Research ◽

10.1161/res.115.suppl_1.11 ◽

2014 ◽

Vol 115 (suppl_1) ◽

Author(s):

Xianmei Meng ◽

Anne Knopf ◽

Agustin Vega-Crespo ◽

James Byrne ◽

Ben Van Handel ◽

...

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Progenitor Cells ◽

Pluripotent Stem Cell ◽

Single Cells ◽

Embryonic Stem ◽

Human Pluripotent Stem Cell ◽

Differentiation Potential ◽

Cell Based Therapy ◽

Trilineage Differentiation

Background: Human pluripotent stem cell-derived cardiovascular progenitor cells (hPSC-CPCs) represent a tractable option for cell-based therapy for heart disease. However, to be clinically relevant, these cells must be derived under good manufacturing practices (GMP)-compatible conditions and produced in great enough quantities to treat adult patients. Here we sought to demonstrate for the first time the generation and expansion of clinically relevant numbers of hPSC-CPCs in xenogen-free protocol. Methods and Results: GMP-grade human induced pluripotent stem cells (GMP-hiPSCs) and human embryonic stem cells (H1 and H9) were dissociated into single cells and cultured in low attachment dishes to differentiate into CPCs in StemPro medium including small molecules and human cytokines with high efficiency of 86%, 80% and 66% for GMP-hiPSCs, H1 and H9, respectively (Figure 1). All hPSC-CPCs possessed trilineage differentiation potentials, as shown by differentiation into endothelial and smooth muscle cells and functional cardiomyocytes (Figure 2). Moreover, sorted hPSC-CPCs expanded >5 fold in 10 days in xenogen-free conditions while still maintaining trilineage differentiation potential and an efficiency of ~70% (Figure 3). Conclusions: Here we demonstrate a xenogeny-free CPC derivation and expansion protocol that can generate clinically relevant numbers of GMP-grade cardiovascular progenitors that could be used in a clinical setting.

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A fossil Aspergillus from Eocene Dominican amber

Journal of Paleontology ◽

10.1017/s0022336000058996 ◽

1988 ◽

Vol 62 (01) ◽

pp. 141-143 ◽

Cited By ~ 15

Author(s):

Gerard M. Thomas ◽

George O. Poinar

Keyword(s):

Dominican Republic ◽

Fossil Species ◽

First Report ◽

Dominican Amber ◽

Eocene Amber

A sporulating Aspergillus is described from a piece of Eocene amber originating from the Dominican Republic. The Aspergillus most closely resembles a form of the white spored phase of Aspergillus janus Raper and Thom. This is the first report of a fossil species of Aspergillus.

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