scholarly journals Impact of seed-born Colletotrichum lindemuthianum on French bean seed quality

2000 ◽  
Vol 34 (4) ◽  
pp. 283-291 ◽  
Author(s):  
S. Ravi ◽  
Sabitha Doraiswamy ◽  
V. Valluvaparadisan ◽  
C. Jeyalakshmi
Keyword(s):  
Seed Quality ◽  
French Bean ◽  
Colletotrichum Lindemuthianum ◽  
Bean Seed

scholarly journals Molecular detection of Colletotrichum lindemuthianum in bean seed samples

2018 ◽  
Vol 40 (4) ◽  
pp. 370-377 ◽  
Author(s):  
Stélio Jorge Castro Gadaga ◽  
Carolina da Silva Siqueira ◽  
José da Cruz Machado
Keyword(s):  
Common Bean ◽  
Molecular Detection ◽  
Seed Quality ◽  
Colletotrichum Lindemuthianum ◽  
The Other ◽  
Inoculum Potential ◽  
Bean Seed ◽  
Detection Techniques ◽  
Pcr Techniques ◽  
Detection And Quantification

Abstract: Colletotrichum lindemuthianum is the causal agent of anthracnose in common bean, and infected seeds are the most typical propagation form of the disease. Thus, using common bean seeds free of C. lindemuthianum is crucial to managing this pest, as well as employing fast and accurate detection techniques to ensure high seed quality. In this study, both conventional and quantitative PCR techniques (cPCR and qPCR) were used for the detection and quantification of C. lindemuthianum in samples of common bean seeds. For that, seeds were inoculated by exposing them to fungal colonies for different periods of time, 0 h, 36 h, 72 h, 108 h and 144 h, each period corresponding to an inoculum potential. Then, they were mixed with healthy seeds, so incidences of 0.25%, 0.50%, 1%, 10%, and 100% of seeds with different inoculum potentials were obtained, in samples of 400 seeds. Both cPRC and qPCR techniques were effective in detecting the fungus. With the cPCR method, the highest sensitivity was recorded in those samples with 10% inoculated seeds with inoculum potential P36. On the other hand, with the qPCR technique, the highest sensitivity in detecting the fungus was observed in samples with 0.25% inoculated seeds with inoculum potential P36.

Effect of microwave radiation on dry bean seed infected with Colletotrichum lindemuthianum with and without the use of chemical seed treatment

2014 ◽  
Vol 94 (8) ◽  
pp. 1373-1384
Author(s):  
A. P. Friesen ◽  
R. L. Conner ◽  
D. E. Robinson ◽  
W. R. Barton ◽  
C. L. Gillard
Keyword(s):  
Microwave Radiation ◽  
Return On Investment ◽  
Seed Treatment ◽  
Seed Quality ◽  
Field Studies ◽  
Microwave Treatment ◽  
Colletotrichum Lindemuthianum ◽  
Bean Seed ◽  
Navy Bean ◽  
Dry Bean

Friesen, A. P., Conner, R. L., Robinson, D. E., Barton, W. R. and Gillard, C. L. 2014. Effect of microwave radiation on dry bean seed infected with Colletotrichum lindemuthianum with and without the use of chemical seed treatment. Can. J. Plant Sci. 94: 1373–1384. Seed-borne anthracnose, caused by Colletotrichum lindemuthianum, is a serious disease that affects dry bean (Phaseolus vulgaris L.) seed quality and yield. A 2-yr study was conducted to examine the effect of microwave radiation on two bean cultivars, Navigator (navy bean) and AC Ole (pinto bean). In the laboratory, a maximum exposure time between 40–50 s caused <10% decrease in seed germination and 0.14 and 0.10% s−1 decrease in pathogen viability for the Navigator and AC Ole cultivars, respectively. Field studies were conducted at Ridgetown and Exeter, ON, to evaluate the effect of microwave radiation and two chemical seed treatments (thiamethoxam+fludioxonil+metalaxyl-M+azoxystrobin and pyraclostrobin+fluxapyroxad+metalaxyl), on emergence, percentage of infection, seed pick, yield and return on investment. Microwave treatment decreased emergence by <10% in both cultivars and decreased disease symptoms by 17–23% for leaf and stem infection in cv. AC Ole when combined with chemical seed treatment. Microwave treatment did not affect pod infection, seed pick, yield, or return on investment, yet chemical treatment increased yield between 9 and 25% in cv. Navigator.

Effects of French Bean Tissue and Leaf Protoplasts on Colletotrichum lindemuthianum Polygalacturonase

1981 ◽  
Vol 102 (3-4) ◽  
pp. 238-246 ◽  
Author(s):  
F. Cervone ◽  
T. Andebrhan ◽  
R. H. A. Coutts ◽  
R. K. S. Wood
Keyword(s):  
French Bean ◽  
Colletotrichum Lindemuthianum ◽  
Leaf Protoplasts

Dry Bean and Anthracnose Development From Seeds With Varying Symptom Severity

Plant Disease ◽  
2020 ◽  
pp. PDIS-02-20-0402
Author(s):  
Jessica M. Halvorson ◽  
Robin S. Lamppa ◽  
Kristin Simons ◽  
Robert L. Conner ◽  
Julie S. Pasche
Keyword(s):  
Pathogen Detection ◽  
Seed Quality ◽  
Transmitted Disease ◽  
Weather Conditions ◽  
Field Trials ◽  
Colletotrichum Lindemuthianum ◽  
Dry Bean ◽  
Yield And Quality ◽  
Moderate Disease ◽  
Pathogen Colonization

Anthracnose, caused by the fungal pathogen Colletotrichum lindemuthianum, is a damaging seed-transmitted disease of dry beans that causes reduced seed quality and yield. Seed-to-seedling transmission of C. lindemuthianum has been documented as high as 15% in asymptomatic seeds under greenhouse conditions. Increasing pathogen colonization in seeds has been correlated with increasing anthracnose seed symptoms via quantitative PCR (qPCR), but stem colonization has not been quantified. Previous studies also have characterized seed yield and quality losses caused by planting C. lindemuthianum–infected seeds, but none evaluated the effect of growing asymptomatic seeds on disease and plant development under field conditions. A real-time qPCR assay was developed in this study and used to detect C. lindemuthianum in the stems of seedlings as early as 15 days after planting. Field trials measured the seed-to-seedling transmission of C. lindemuthianum across levels of anthracnose symptoms in seeds ranging from healthy to severely discolored. Results from these two field trials indicated that emergence and yield decreased and foliar symptoms, pathogen detection, and incidence of symptoms on progeny seeds increased as the severity of infection in planted seeds increased. In both years, planting asymptomatic seeds resulted in higher anthracnose severity than planting healthy seeds. Yield, seed weight, and incidence of symptoms on progeny seeds were not higher in asymptomatic seeds than in healthy seeds in 2014, when moderate disease pressure was observed. However, these factors were significantly different in 2015, when anthracnose severity was driven up to 75% by conducive weather conditions. This serves as a strong warning to growers that planting seed grown in a field where anthracnose was present, even if those seeds are asymptomatic, can result in yield and quality losses. Planting certified dry bean seed is always recommended.

Factors affecting seed quality in dwarf French bean (Phaseolus vulgaris L.) before harvest maturity

2004 ◽  
Vol 32 (3) ◽  
pp. 797-811 ◽  
Author(s):  
M.M. Greven ◽  
B.A. McKenzie ◽  
J.G. Hampton ◽  
M.J. Hill ◽  
J.R. Sedcole ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Seed Quality ◽  
French Bean ◽  
Phaseolus Vulgaris L ◽  
Factors Affecting ◽  
Harvest Maturity

scholarly journals Phytoferritin is synthesized in vitro as a high-molecular-weight precursor. Studies on the synthesis and the uptake in vitro of the precursors of ferritin and ferredoxin by intact chloroplasts

1983 ◽  
Vol 214 (3) ◽  
pp. 943-950 ◽  
Author(s):  
F van der Mark ◽  
W van den Briel ◽  
H G Huisman
Keyword(s):  
Gel Electrophoresis ◽  
Biosynthetic Pathway ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
French Bean ◽  
Polyacrylamide Gel ◽  
Cell Organelle ◽  
Bean Seed ◽  
Intact Chloroplasts

Evidence is presented that French-bean (Phaseolus vulgaris) seed ferritin is composed of one type of subunit with an apparent Mr of 26500. In normal and iron-loaded leaf tissues it is detected immunologically with an antiserum raised against purified bean seed ferritin and migrates in SDS (sodium dodecyl sulphate)/polyacrylamide-gel electrophoresis with the same mobility as the bean seed ferritin subunit. The biosynthetic pathway of ferritin in normal and iron-loaded leaves was investigated. RNA was extracted, fractionated into polyadenylated RNA and translated in a cell-free rabbit reticulocyte lysate and a wheat-germ-extract system. The products were identified by SDS/polyacrylamide-gel electrophoresis after indirect immunoprecipitation. In all cases the ferritin product had an Mr 5000 higher than that of the native subunit. Uptake and processing of the precursor form of ferritin from iron-loaded leaves by intact chloroplasts was demonstrated. This indicates that, in iron-loaded leaves, ferritin acts as a chloroplast protein. We propose that the ferritin precursor in normal leaves follows the same biosynthetic pathway. This suggests that the iron-buffering function of ferritin in plants takes place in the chloroplast and that non-functional cellular iron will accumulate in this cell organelle.

scholarly journals Impact of Organic Seed Priming Amendments and Botanicals on Seed Quality Parameters of French Bean (Phaseolous vulgaris L.)

2021 ◽  
Vol 10 (11) ◽  
pp. 259-265
Author(s):  
Prashant Kumar Rai Kotamarthi Bhavana ◽  
G. M. Lal
Keyword(s):  
Leaf Extract ◽  
Seed Quality ◽  
Low Cost ◽  
Organic Amendments ◽  
Seed Priming ◽  
Quality Parameters ◽  
French Bean ◽  
Distilled Water ◽  
Seed Vigour ◽  
Vigour Index

The current experiment was conducted to evaluate the Performance of Organic amendments and botanical treatments on seed quality parameters of French bean under stimulated environmental conditions. An experiment was conducted in 2021 at postgraduate seed testing laboratory, Department of Genetics and Plant Breeding Naini Agriculture Institute, Sam Higginbottom University of Agriculture Technology and Sciences, Prayagraj. The French bean Cultivar Arka Bold seeds were used for priming. Organic treatments are designated as Panchagavya 2%, 4%,6%,8%, Agniasthra 2%,4%,6%,8% and Botanicals treatments are Tulasi leaf extract 5%, Aloevera leaf extract 5%, Vinca rosea leaf extract 5% and also did hydroprming treatment using distilled water respectively. Seeds were soaked in organic, botanicals and in distilled water for duration of 6 hours followed by shade drying. The results were obtained and it was found that all the treatments showed significant effect on quality parameters. The highest was found in panchgavya of all doses followed by tulasi leaf extract found to be best in Germination percentage, Speed of Germination, Root length, Shoot length, Seedling length, Seed vigour index-I, Seed vigour Index-II compared to control or untreated seeds which indicates the effect of organics and botanicals on physiological processes of the seeds. This study revealed that priming with organic amendments and botanicals can be effective in enhancing the seed quality parameters of french bean and they are low cost, effective and nature friendly and toxic free.

scholarly journals Effect of Storage Containers and Storage Periods on The Seed Quality of French Bean (Phaseolus vulgaris)

2012 ◽  
Vol 37 (2) ◽  
pp. 195-205 ◽  
Author(s):  
KM Khalequzzaman ◽  
MM Rashid ◽  
MA Hasan ◽  
MA Reza
Keyword(s):  
Moisture Content ◽  
Seed Weight ◽  
Seed Quality ◽  
French Bean ◽  
Vigour Index ◽  
Seed Rot ◽  
Storage Containers ◽  
Abiotic And Biotic Factors ◽  
And Storage

The experiment was conducted in the Laboratory, Department of Plant Pathology,  HSTU, Dinajpur during April – July 2010 to know the effect of abiotic and biotic  factors, storage periods and storage containers on the seed quality of French  bean. Tin container showed the highest germination, normal seedlings and  vigour index which were followed by polythene bag, where Gunny bag showed  the lowest germination, normal seedlings and vigour index upto 60 days after  storage. The highest 1000-seed weight, moisture content, abnormal seedlings,  seed rot and incidence of the Fusarium oxysporum were recorded in Gunny bag,  where the lowest of these parameters were recorded in Tin container. Seed  colour of Tin container were more or less same as initial colour, but seeds of  Gunny bag were changed into fade or brown colour at 60 days after storage.  Moisture content, 1000-seed weight, abnormal seedlings, seed rot, fungi  association were increased, but germination and normal seedlings were  decreased with the increase of storage periods. Among the three containers, Tin  container was the best and the Gunny bag was the worst storage containers upto  60 days of storage for French bean seed. DOI: http://dx.doi.org/10.3329/bjar.v37i2.11221 Bangladesh J. Agril. Res. 37(2): 195-205, June 2012  

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