In vitro study of electron beam deposited calcium phosphate coating in simulated body fluid

2007 ◽  
Vol 22 (3) ◽  
pp. 621-626
Author(s):  
M. Hamdi ◽  
Ari Ide-Ektessabi ◽  
J.A. Toque
Keyword(s):  
Electron Beam ◽  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
In Vitro Study ◽  
Calcium Phosphate Coating ◽  
Carbonate Content ◽  
Apatite Formation ◽  
Calcium Phosphate Coatings

Calcium phosphate coatings prepared using the technique of electron beam deposition were immersed in a simulated body fluid for different periods of time to determine their response in vitro. The amorphous as-deposited coatings dissolved completely after a few days of immersion. After annealing in air at 700 °C, the dissolution of a small amount of amorphous phase in the crystalline coatings promotes the precipitation of bonelike apatite on the recessed regions by increasing the local supersaturation of calcium and phosphate ions. Formation of apatite was confirmed by the x-ray diffraction peaks at (200), (211), and (203) planes which grew after immersion in simulated body fluid. Fourier transform infrared results conformed to this with the increase in intensity of the absorption band at 1450 cm−1, signifying the increase in carbonate content. Scanning electron microscopy results showed spherical-shaped apatite nucleated on dissolved surface after 8 days of immersion. Sixteen days after immersion, almost 80% of the surface area was covered with apatite formation and grew to coalesce between neighboring particles forming an integrated platelike layer after 28 days. No obvious detachment between the grown layer and the underlying coating was observed.

In Vitro Study Bioactivity of HA/UHMWPE Nanocomposites

2007 ◽  
Vol 342-343 ◽  
pp. 701-704
Author(s):  
Li Ming Fang ◽  
Yang Leng ◽  
Ping Gao
Keyword(s):  
Molecular Weight ◽  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
In Vitro Study ◽  
Ultrahigh Molecular Weight Polyethylene ◽  
Octacalcium Phosphate ◽  
Tof Sims ◽  
Ultrahigh Molecular Weight

Bioactivity of hydroxyapatite reinforced ultrahigh molecular weight polyethylene (HA/UHMWPE) nanocomposites with HA volume content of 10~50 % was evaluated by simulated body fluid (SBF) immersion. The effect of HA content on the capability for calcium phosphate (Ca- P) induction was studied. It was found that Ca-P deposition covered the whole surface of the composite with 30 vol. % of HA after immersion for 1 day and the layer grew to around 10 0m thick in one-week immersion, while there was few nucleus formed for composites with HA content lower than 30 vol. % after one-week immersion. The Ca-P structure was identified as octacalcium phosphate (OCP) by SEM, TEM, and ToF-SIMS.

Growth of Calcium Phosphate Coating on Ti-7.5Mo Alloy after Anodic Oxidation

2013 ◽  
Vol 334-335 ◽  
pp. 297-302 ◽  
Author(s):  
A.L.A. Escada ◽  
João Paulo Barros Machado ◽  
Roberto Zenhei Nakazato ◽  
Ana Paula Rosifini Alves Claro
Keyword(s):  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Calcium Phosphates ◽  
Sodium Titanate ◽  
Ammonium Fluoride ◽  
Biological Properties ◽  
Calcium Phosphate Coating ◽  
Phosphate Coating

Titanium and its alloys are widely used as biomaterials due to their mechanical, chemical and biological properties. To enhance the biocompatibility of titanium alloys, various surface treatments have been proposed. In particular, the formation of titanium oxide nanotubes layers has been extensively examined. Among the various materials for implants, calcium phosphates and hydroxyapatite are widely used clinically. In this work, titanium nanotubes were fabricated on the surface of Ti-7.5Mo alloy by anodization. The samples were anodized for 20 V in an electrolyte containing glycerol in combination with ammonium fluoride (NH4F, 0.25%), and the anodization time was 24 h. After being anodized, specimens were heat treated at 450 °C and 600°C for 1 h to crystallize the amorphous TiO2 nanotubes and then treated with NaOH solution to make them bioactive, to induce growth of calcium phosphate in a simulated body fluid. Surface morphology and coating chemistry were obtained respectively using, field-emission scanning electron microscopy (FEG-SEM), AFM and X-ray diffraction (XRD). It was shown that the presence of titanium nanotubes induces the growth of a sodium titanate nanolayer. During the subsequent in-vitro immersion in a simulated body fluid, the sodium titanate nanolayer induced the nucleation and growth of nanodimensioned calcium phosphate. It was possible to observe the formation of TiO2 nanotubes on the surface of Ti-7.5Mo. Calcium phosphate coating was greater in the samples with larger nanotube diameter. These findings represent a simple surface treatment for Ti-7.5Mo alloy that has high potential for biomedical applications.

scholarly journals In Vitro Degradation Behaviors of Manganese-Calcium Phosphate Coatings on an Mg-Ca-Zn Alloy

Scanning ◽  
2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Yichang Su ◽  
Yingchao Su ◽  
Wei Zai ◽  
Guangyu Li ◽  
Cuie Wen
Keyword(s):  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Electrochemical Corrosion ◽  
Hydrogen Release ◽  
Phosphate Coatings ◽  
Degradation Behaviors ◽  
Calcium Phosphate Coatings ◽  
Zn Alloy

In order to decrease the degradation rate of magnesium (Mg) alloys for the potential orthopedic applications, manganese-calcium phosphate coatings were prepared on an Mg-Ca-Zn alloy in calcium phosphating solutions with different addition of Mn2+. Influence of Mn content on degradation behaviors of phosphate coatings in the simulated body fluid was investigated to obtain the optimum coating. With the increasing Mn addition, the corrosion resistance of the manganese-calcium phosphate coatings was gradually improved. The optimum coating prepared in solution containing 0.05 mol/L Mn2+ had a uniform and compact microstructure and was composed of MnHPO4·3H2O, CaHPO4·2H2O, and Ca3(PO4)2. The electrochemical corrosion test in simulated body fluid revealed that polarization resistance of the optimum coating is 36273 Ωcm2, which is about 11 times higher than that of phosphate coating without Mn addition. The optimum coating also showed the most stable surface structure and lowest hydrogen release in the immersion test in simulated body fluid.

IN-VITRO BEHAVIOUR OF BIPHASIC CALCIUM PHOSPHATE WITH DIFFERENT RATIO OF SILICA CONTENT IN SIMULATED BODY FLUID

2015 ◽  
Vol 23 (1) ◽  
pp. 1-14
Author(s):  
Sudirman Sahid ◽  
◽  
Nor Shahida Kader Bashah ◽  
Salina Sabudin ◽  
◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Biphasic Calcium Phosphate ◽  
Silica Content

Biomimetic Mineralization and Bioactivity of Phosphorylated Chitosan

2005 ◽  
Vol 288-289 ◽  
pp. 429-432 ◽  
Author(s):  
Zhi Qing Chen ◽  
Quan Li Li ◽  
Quan Zen ◽  
Gang Li ◽  
Hao Bin Jiang ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Crystal Nucleation ◽  
Osteoblast Proliferation ◽  
Biomimetic Mineralization ◽  
P Content ◽  
Proliferation And Differentiation ◽  
Nucleation Growth

Phosphorylated chitosans were synthesized as templates to manipulate hydroxyapatite (HA) crystal nucleation, growth and microstructure. Two kinds of insoluble phosphorylated chitosan were soaked in saturated Ca(OH)2 solution for 4 d and in 1.5× SBF (simulated body fluid) solutions for 14 d at 37 °C for biomimetic mineralization. A lower [P]-content of phosphorylated chitosan promoted greater mineralization than higher [P]-content. Phosphorylated chitosan inhibited osteoblast proliferation and differentiation in vitro, while calcium phosphate phosphorylated chitosan composites did not.

Evaluation of surface modification techniques on the ability of apatite formation and corrosion behavior in synthetic body fluid: An in vitro study

2021 ◽  
Vol 22 ◽  
pp. 100866
Author(s):  
Cosmin M. Cotrut ◽  
Ionut C. Ionescu ◽  
Elena Ungureanu ◽  
Andrei Berbecaru ◽  
Raluca I. Zamfir ◽  
...  
Keyword(s):  
Surface Modification ◽  
Corrosion Behavior ◽  
Body Fluid ◽  
In Vitro Study ◽  
Vitro Study ◽  
Apatite Formation ◽  
Surface Modification Techniques

Composite Calcium Phosphate Coatings on Mg Alloy for Medicine

2015 ◽  
Vol 245 ◽  
pp. 159-165 ◽  
Author(s):  
Sergey V. Gnedenkov ◽  
Sergey L. Sinebryukhov ◽  
Artyom М. Puz' ◽  
Dmitry V. Mashtalyar ◽  
Natalya G. Plekhova
Keyword(s):  
Calcium Phosphate ◽  
Biologically Active ◽  
Mg Alloy ◽  
High Tech ◽  
Calcium Phosphate Coating ◽  
Phosphate Coatings ◽  
Calcium Phosphate Layer ◽  
Plasma Electrolytic ◽  
Calcium Phosphate Coatings

The high-tech method of creating anticorrosion calcium phosphate coating on the magnesium alloy MA8 (MgMnCe) has been developed. As was demonstrated by the volumetry method the sealing of the layer formed on the surface of Mg alloy using plasma electrolytic oxidation by superdispersed polytetrafluoroethylene substantially reduced the rate of the corrosion process. Here, the surface of the calcium phosphate layer containing hydroxyapatite (Ca/P = 1.61) remains biologically active. Studies of architectonics of the surface of innate immune cells have been performed in vitro.

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