Europium(III)-induced water-soluble nano-aggregates of hyaluronic acid and chitosan: structure and fluorescence

Hyaluronic acid (HA) has been known to play an important role in wound healing process. However, the effect of molecular weight (MW) of exogenously administered HA on the wound healing process has not been fully understood. In this study, we investigated HA with different MWs on wound healing process using human epidermal keratinocytes and dermal fibroblasts. Cell proliferation and migration ability were assessed by water soluble tetrazolium (WST) assay and wound scratch assay. We examined the effect of HA addition in a full-thickness wound model in mice and the gene expression related to wound healing. Proliferation and migration of HaCaT cells increased with the increase of MW and concentration of HA. Interleukin (IL-1β), IL-8 and vascular endothelial growth factor (VEGF) as well as matrix metalloproteinase (MMP)-9 and MMP-13 were significantly upregulated by high molecular weight (HMW) HA in keratinocytes. Together with VEGF upregulation and the observed promotion of HaCaT migration, HA with the MW of 2290 kDa may hold potential to improve re-epithelialization, a critical obstacle to heal chronic wounds.

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Preparation of cross-linked hyaluronic acid film using 2-chloro-1-methylpyridinium iodide or water-soluble 1-ethyl-(3,3-dimethylaminopropyl)carbodiimide

Journal of Biomaterials Science Polymer Edition ◽

10.1163/156856204774196153 ◽

2004 ◽

Vol 15 (6) ◽

pp. 767-780 ◽

Cited By ~ 36

Author(s):

Jenn-Jong Young ◽

Kuang-Ming Cheng ◽

Tai-Li Tsou ◽

Hwan-Wun Liu ◽

Hsian-Jenn Wang

Keyword(s):

Hyaluronic Acid ◽

Water Soluble

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Water-Soluble Blue Fluorescent Nonconjugated Polymer Dots from Hyaluronic Acid and Hydrophobic Amino Acids

ACS Omega ◽

10.1021/acsomega.1c01343 ◽

2021 ◽

Author(s):

Deep S. Bhattacharya ◽

Aishwarya Bapat ◽

Denis Svechkarev ◽

Aaron M. Mohs

Keyword(s):

Amino Acids ◽

Hyaluronic Acid ◽

Water Soluble ◽

Polymer Dots ◽

Hydrophobic Amino Acids

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New Carbohydrate-Based Polymeric Materials

MRS Bulletin ◽

10.1557/s0883769400046492 ◽

1992 ◽

Vol 17 (10) ◽

pp. 54-59 ◽

Cited By ~ 1

Author(s):

Matthew R. Callstrom ◽

Mark D. Bednarski

Keyword(s):

Food Additives ◽

Polymeric Materials ◽

Water Soluble ◽

Natural Polymers ◽

Water Soluble Polymers ◽

Soluble Polymers ◽

Chemically Modified ◽

Stereochemical Control ◽

Natural Polysaccharide ◽

Image Position

The total world production of water-soluble polymers is estimated to be greater than five million tons per year. Water-soluble polymers are most conveniently described according to their origin in three classes (see Structures 1-6):∎ Natural polymers, including starch (1) and cellulose (2);∎ chemically modified natural polymers, including, for example, hydroxyethyl starch (3) and cellulose acetate (4); and∎ synthetic polymers, the most important of which are polyacrylamide (5) and polyvinyl alcohol (6), (commonly composed of both alcohol and acetate groups as shown). The widespread use of these materials is due to both their availability and the range of useful physical properties found in the various natural and chemically modified natural polymers.Of the commercial water-soluble polymers, approximately 50–80% are based on natural polysaccharide materials. One of the primary reasons that these materials find such widespread use is the dramatic response of their properties to changes in their functionality and stereochemistry: chemical modification or the combination of polysaccharides with other polymeric materials has yielded materials whose applications range from explosives to food additives. Although efforts directed at controlling the properties of polysaccharides has resulted in a wide variety of useful materials, we felt control of the composition of carbohydrate-based polymers at the molecular level would provide materials with properties superior to those derived from natural and chemically modified polysaccharide materials.Our approach for the preparation of new carbohydrate-based materials is to use the carbohydrate as a template for the introduction of desired functionality with complete regiochemical and stereochemical control by both chemical and enzymatic methods (Scheme I).

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ENHANCEMENT OF THE SOLUBILITY OF FAMOTIDINE SOLID DISPERSION USING NATURAL POLYMER BY SOLVENT EVAPORATION

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2021v13i3.40934 ◽

2021 ◽

pp. 193-198

Author(s):

HUSSEIN K. ALKUFI ◽

ASMAA M. RASHID

Keyword(s):

Hyaluronic Acid ◽

Solid Dispersion ◽

Solid Dispersions ◽

Solvent Evaporation ◽

Water Soluble ◽

Poorly Soluble Drugs ◽

Natural Polymer ◽

Solubility Study ◽

Poorly Soluble ◽

Pure Drug

Objective: The aims of the study to enhance solubility and dissolution of famotidine using natural polymer. Solubility study of a drug is one of the contributing factors of its oral bioavailability. The formulation of poorly soluble drugs for oral delivery presents a challenge to the formulation technologists. Methods: The present study has shown that it is possible to raise the solubility for poorly soluble drugs like famotidine, by preparing solid dispersion using natural water-soluble polymer (xyloglucan and hyaluronic acid) as solubilizer through solvent evaporation method. Physical mixture and solid dispersion of famotidine with xyloglucan (XG) or hyaluronic acid in a ratio of 1:1, 1:2, 1:3 were prepared. Solubility study, drug content, dissolution profile and compatibility study were performed for famotidine in solid dispersions XS1, XS2, XS3, HS4, HS5, HS6 as well as in physical mixtures at a ratio 1:1 for both polymer (XG and hyaluronic acid). Results: It was observed that solid dispersions of each drugs showed an increase in dissolution rate in comparison with its pure drug in the ratio of 1:1 (Drug: carrier). It can be concluded that with the care and proper use of xyloglucan, the solubility of drugs poorly soluble can be improved. The prepared solid dispersion showed improvement of drug solubility in all prepared formulas. The best result was obtained with formula XS1 (famotidine: xyloglucan at ratio 1:1) that showed 26 fold increase in solubility compared to the solubility of pure drug. Conclusion: The natural solid dispersion, increased wettability and reduced crystallinity of the drug which leads to improving solubility and dissolution.

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