Multiple Phage-Based Magnetoelastic Biosensors System for the Detection of Salmonella typhimurium and Bacillus anthracis Spores

2008 ◽  
Vol 1129 ◽  
Author(s):  
S. Huang ◽  
S. Li ◽  
H. Yang ◽  
M. L. Johnson ◽  
Ramji S Lakshmanan ◽  
...  
Keyword(s):  
Resonance Frequency ◽  
Rapid Detection ◽  
Detection System ◽  
Additional Mass ◽  
Response Curves ◽  
Biosensor System ◽  
Bacillus Anthracis Spores ◽  
In Situ Detection ◽  
Multiple Pathogens

AbstractThis paper presents a multiple magnetoelastic (ME) biosensor system for in-situ detection of S. typhimurium and B. anthracis spores in a flowing bacterial/spore suspension (5 x 101 - 5 x 108 cfu/ml). The ME biosensor was formed by immobilizing filamentous phage (specific to each detection target) on the ME platforms. An alternating magnetic field was used to resonate the ME biosensor to determine its resonance frequency. When cells/spores are bound to a ME biosensor surface, the additional mass of the cells/spores causes a decrease in the resonance frequency of the biosensor. The detection system was composed of a control sensor, an E2 phage-based biosensor (specific to S. typhimurium) and a JRB7 phage-based biosensor (specific to B. anthracis spores). The frequency response curves of the ME biosensors as a function of exposure time were then measured and the detection limit of the ME biosensor was observed to be 5 x 103 cfu/ml. The results show that phage-based ME biosensors can detect multiple pathogens simultaneously and offer good performance, including good sensitivity and rapid detection.

scholarly journals Real Time In-situ Detection System Research for Methane in Offshore Shallow Gas based on Thin Film Interface

2018 ◽  
Vol 232 ◽  
pp. 04053
Author(s):  
Cheng-xing Miao ◽  
Qing Li ◽  
Sheng-yao Jia
Keyword(s):  
Real Time ◽  
Detection Efficiency ◽  
Detection System ◽  
Detection Methods ◽  
Shallow Gas ◽  
Micro Gas Sensor ◽  
Detection Probe ◽  
On Line ◽  
In Situ Detection

In order to get ridded of the non real-time detection methods of artificial site sampled and laboratory instrument analyzed in the field of methane detection in the offshore shallow gas, real-time in-situ detection system for methane in offshore shallow gas was designed by the film interface.The methane in the offshore shallow gas through the gas-liquid separation membrane of polymer permeation into the system internal detection probe, analog infrared micro gas sensor sensed the methane concentration and the corresponded output value, data acquisition and communication node fitted into standard gas concentration.Based on the experimental data compared with the traditional detection method, and further analyzed the causes of error produced by the case experiment. The application results show that the system can achieve a single borehole layout, long-term on-line in-situ on-line detection, and improve the detection efficiency and the timeliness of the detection data.

scholarly journals In situ detection of live-to-dead bacteria ratio after inactivation by means of synchronous fluorescence and PCA

2018 ◽  
Vol 115 (4) ◽  
pp. 668-673 ◽  
Author(s):  
Runze Li ◽  
Umang Goswami ◽  
Maria King ◽  
Jie Chen ◽  
Thomas C. Cesario ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Rapid Detection ◽  
Fluorescence Spectra ◽  
Uv Light ◽  
Principal Component ◽  
Synchronous Fluorescence ◽  
Health Care Associated Infection ◽  
In Situ Detection

The determination of live and dead bacteria is of considerable significance for preventing health care-associated infection in hospitals, field clinics, and other areas. In this study, the viable (live) and nonviable (dead) bacteria in a sample were determined by means of their fluorescence spectra and principal component analysis (PCA). Data obtained in this study show that it is possible to identify bacteria strains and determine the live/dead ratio after UV light inactivation and antibiotic treatment, in situ, within minutes. In addition, synchronous fluorescence scans enable the identification of bacterial components such as tryptophan, tyrosine, and DNA. Compared with the time-consuming plating and culturing methods, this study renders a means for rapid detection and determination of live and dead bacteria.

scholarly journals A New Integrated Multi-Pathogen In Situ Detection Platform Using Electrochemical Impedance Spectroscopy

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 1506
Author(s):  
Karlheinz Kellner ◽  
Jörg Ettenauer ◽  
Martin Brandl
Keyword(s):  
Electrochemical Impedance Spectroscopy ◽  
Impedance Spectroscopy ◽  
Pathogenic Bacteria ◽  
Electrochemical Impedance ◽  
Cell Model ◽  
Successful Implementation ◽  
Eis Measurements ◽  
In Situ Detection ◽  
Multiple Pathogens

Scientists are always searching for inventions and developing new methods for the detection of pathogenic bacteria in drinking water. Some of those techniques are based on electrochemical reactions, performed by a device called potentiostat. For in situ analysis, there is a high demand for hardware efficient instruments with a customized set of requirements including Electrochemical Impedance Spectroscopy (EIS) measurements. Therefore, we developed the ‘EcoStat’ device. As outcome of further developments, we demonstrate the successful implementation of an EIS feature and a multiplexing unit to achieve a detection platform for multiple pathogens. We present results performed on a simplified Randles Cell model.

An autonomous in situ detection system for radioactivity measurements in the marine environment

2008 ◽  
Vol 66 (10) ◽  
pp. 1419-1426 ◽  
Author(s):  
C. Tsabaris ◽  
C. Bagatelas ◽  
Th. Dakladas ◽  
C.T. Papadopoulos ◽  
R. Vlastou ◽  
...  
Keyword(s):  
Marine Environment ◽  
Detection System ◽  
In Situ Detection ◽  
Radioactivity Measurements

scholarly journals Machine-Learning Assisted Discrimination of Precancerous and Cancerous from Healthy Oral Tissue Based on Multispectral Autofluorescence Lifetime Imaging Endoscopy

Cancers ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 4751
Author(s):  
Elvis Duran-Sierra ◽  
Shuna Cheng ◽  
Rodrigo Cuenca ◽  
Beena Ahmed ◽  
Jim Ji ◽  
...  
Keyword(s):  
Machine Learning ◽  
Detection System ◽  
Characteristic Curve ◽  
Oral Epithelial Dysplasia ◽  
Lifetime Imaging ◽  
Clinical Center ◽  
Oral Tissue ◽  
In Situ Detection ◽  
Oral Epithelial

Multispectral autofluorescence lifetime imaging (maFLIM) can be used to clinically image a plurality of metabolic and biochemical autofluorescence biomarkers of oral epithelial dysplasia and cancer. This study tested the hypothesis that maFLIM-derived autofluorescence biomarkers can be used in machine-learning (ML) models to discriminate dysplastic and cancerous from healthy oral tissue. Clinical widefield maFLIM endoscopy imaging of cancerous and dysplastic oral lesions was performed at two clinical centers. Endoscopic maFLIM images from 34 patients acquired at one of the clinical centers were used to optimize ML models for automated discrimination of dysplastic and cancerous from healthy oral tissue. A computer-aided detection system was developed and applied to a set of endoscopic maFLIM images from 23 patients acquired at the other clinical center, and its performance was quantified in terms of the area under the receiver operating characteristic curve (ROC-AUC). Discrimination of dysplastic and cancerous from healthy oral tissue was achieved with an ROC-AUC of 0.81. This study demonstrates the capabilities of widefield maFLIM endoscopy to clinically image autofluorescence biomarkers that can be used in ML models to discriminate dysplastic and cancerous from healthy oral tissue. Widefield maFLIM endoscopy thus holds potential for automated in situ detection of oral dysplasia and cancer.

scholarly journals Integrated and Portable Probe Based on Functional Plastic Scintillator for Detection of Radioactive Cesium

2021 ◽  
Vol 11 (11) ◽  
pp. 5210
Author(s):  
Sujung Min ◽  
Hara Kang ◽  
Bumkyung Seo ◽  
Changhyun Roh ◽  
Sangbum Hong ◽  
...  
Keyword(s):  
Plastic Scintillator ◽  
Detection Efficiency ◽  
Direct Detection ◽  
Detection System ◽  
Light Yield ◽  
Radioactive Cesium ◽  
Radiological Measurement ◽  
Measurement Experiment ◽  
In Situ Detection

The highly reliable and direct detection of radioactive cesium has gained potential interest due to in-situ detection and monitoring in environments. In this study, we elucidated an integrated and portable probe based on functional plastic scintillator for detection of radioactive cesium. A functional plastic scintillator with improved detection efficiency was fabricated including CdTe (cadmium telluride) material. Monolith-typed functional plastic scintillator having a diameter of 50 mm and a thickness of 30 mm was manufactured by adding 2,5-diphenyloxazole (PPO, 0.4 wt%), 1,4 di[2-(5phenyloxazolyl)]benzene (POPOP, 0.01 wt%), and CdTe (0.2 wt%) materials in a styrene-based matrix. To evaluate the applicability of the plastic scintillator manufactured to in-situ radiological measurement, an integrated plastic detection system was created, and the measurement experiment was performed using the Cs-137 radiation source. Additionally, detection efficiency was compared with a commercial plastic scintillator. As results, the efficiency and light yield of a functional plastic scintillator including CdTe were higher than a commercial plastic scintillator. Furthermore, the remarkable performance of the functional plastic scintillator was confirmed through comparative analysis with Monte Carlo simulation.

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