High-throughput Detection of Protein Kinase Activities in Cell Lysate Based on the Aggregation of Gold Nanoparticles with Peptides

2009 ◽  
Author(s):  
Yoshiki Katayama ◽  
Hirotaro Kitazaki ◽  
Jeong-Hun Kang ◽  
Xiaoming Han ◽  
Takeshi Mori ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Protein Kinase ◽  
High Throughput ◽  
Cell Lysate ◽  
High Throughput Detection

Study on Gold Nanoparticles Aggravating MEMS Cantilever Gene Detection Biosensor

2013 ◽  
Vol 336-338 ◽  
pp. 148-152
Author(s):  
Yue Tao Ge ◽  
Xiao Tong Yin ◽  
Chao Bang Guo ◽  
Bo Yang
Keyword(s):  
Gold Nanoparticles ◽  
Nucleic Acid ◽  
High Throughput ◽  
Layer By Layer ◽  
Gene Detection ◽  
Detection Level ◽  
Target Dna ◽  
Mems Technology ◽  
High Throughput Detection ◽  
Micro Cantilever

In order to detect whether there are nucleic acid components interested in the testing samples, this study manuscript presents a method and a process based on MEMS technology and biosensor technology. Although MEMS micro cantilever is very sensitive for stress mass, we must aggravate the cantilever for the reason of microscopic DNA mass is very weak. In order to achieve the purpose of their molecules detection level, gold nanoparticles (GNPs) which are combined with hybridization information is taken to aggravate MEMS cantilever, and we employ Au - S chemical bond of GNPs and dithiol HS(CH2)6SH to combine and fix DNA probe, and then employ target DNA which is marked with biotin to combine GNPs by Biotin - Streptavidin combining. We use the method of layer by layer to amplify the signal again until meet the hypersensitized requirements. Last we apply multi-channel microfluidic technology to achieve high-throughput detection.

High-Throughput Detection of an Alkaloidal Plant Metabolome in Plant Extracts Using LC-ESI-QTOF-MS

2021 ◽  
Author(s):  
Bibi Zareena ◽  
Adeeba Khadim ◽  
Syed Usama Y. Jeelani ◽  
Saddam Hussain ◽  
Arslan Ali ◽  
...  
Keyword(s):  
High Throughput ◽  
Plant Extracts ◽  
High Throughput Detection

Continuous detection of trace level concentration of oil droplets in water using microfluidic AC electroosmosis (ACEO)

RSC Advances ◽  
2015 ◽  
Vol 5 (86) ◽  
pp. 70197-70203 ◽  
Author(s):  
D. Das ◽  
Z. Yan ◽  
N. V. Menon ◽  
Y. Kang ◽  
V. Chan ◽  
...  
Keyword(s):  
Oil Spill ◽  
High Throughput ◽  
Ac Electroosmosis ◽  
Oil Droplets ◽  
Trace Level ◽  
High Throughput Detection ◽  
Novel Design

A novel design for high throughput detection of oil micro-droplets in water which is important to environmental oil spill monitoring agencies.

scholarly journals Novel High-Throughput Detection Method To Assess Bacterial Surfactant Production

2010 ◽  
Vol 76 (16) ◽  
pp. 5363-5372 ◽  
Author(s):  
Adrien Y. Burch ◽  
Briana K. Shimada ◽  
Patrick J. Browne ◽  
Steven E. Lindow
Keyword(s):  
Pseudomonas Syringae ◽  
High Throughput ◽  
Water Drop ◽  
Bacterial Strains ◽  
Swarming Motility ◽  
Detection Assay ◽  
Wide Range ◽  
Surfactant Production ◽  
Transposon Mutants ◽  
High Throughput Detection

ABSTRACT A novel biosurfactant detection assay was developed for the observation of surfactants on agar plates. By using an airbrush to apply a fine mist of oil droplets, surfactants can be observed instantaneously as halos around biosurfactant-producing colonies. This atomized oil assay can detect a wide range of different synthetic and bacterially produced surfactants. This method could detect much lower concentrations of many surfactants than a commonly used water drop collapse method. It is semiquantitative and therefore has broad applicability for uses such as high-throughput mutagenesis screens of biosurfactant-producing bacterial strains. The atomized oil assay was used to screen for mutants of the plant pathogen Pseudomonas syringae pv. syringae B728a that were altered in the production of biosurfactants. Transposon mutants displaying significantly altered surfactant halos were identified and further analyzed. All mutants identified displayed altered swarming motility, as would be expected of surfactant mutants. Additionally, measurements of the transcription of the syringafactin biosynthetic cluster in the mutants, the principal biosurfactant known to be produced by B728a, revealed novel regulators of this pathway.

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