scholarly journals Formula padat Bacillus cereus STRAIN TLE1.1 untuk pengendalian penyakit busuk pangkal batang (Sclerotium rolfsii) pada tanaman tomat

Jurnal Agro ◽  
2022 ◽  
Vol 8 (2) ◽  
pp. 226-236
Author(s):  
Yulmira Yanti ◽  
Hasmiandy Hamid ◽  
Reflin Reflin ◽  
Yaherwandi Yaherwandi ◽  
Febri Yani Chrismont
Keyword(s):  
Experimental Study ◽  
Bacillus Cereus ◽  
Solid Waste ◽  
Tomato Plant ◽  
Sclerotium Rolfsii ◽  
Stem Rot ◽  
Tomato Plants ◽  
Tomato Seeds ◽  
Rot Disease ◽  
Almost All

Penyakit utama tanaman tomat yaitu busuk pangkal batang yang disebabkan oleh Sclerotium rolfsii dapat menimbulkan kerugian mencapai 80-100%. Tujuan penelitian yaitu untuk mendapatkan formula padat Bacillus cereus strain TLE1.1 yang efektif untuk pengendalian penyakit busuk pangkal batang pada tanaman tomat. Penelitian bersifat eksperimen dengan mengamati kemampuan formula padat B. cereus strain TLE1.1 dalam pengendalian penyakit busuk pangkal batang dengan Rancangan Acak Lengkap yang terdiri atas 9 perlakuan dan 3 ulangan. Perlakuan terdiri atas kombinasi bahan pembawa formula padat yang terdiri atas limbah padat ampas tebu, ampas tahu dan tongkol jagung, fungisida serta kontrol. Masing-masing formula padat B. cereus strain TLE1.1 diintroduksi pada benih dan bibit tomat. Hasil penelitian menunjukkan bahwa hampir semua formula mampu menekan penyakit busuk pangkal batang tanaman tomat. Formula terbaik dalam menurunkan penyakit busuk pangkal batang pada tanaman yaitu formula ampas tahu dan ampas tahu + tongkol jagung.Main disease of tomato plant, namely stem rot caused by Sclerotium rolfsii which can cause losses up to 80-100%. The aim of the study was to obtain a solid formula of Bacillus cereus strain TLE1.1 which was effective for controlling stem rot disease in tomato plant. This research was an experimental study to know the ability of the solid formula of B. cereus strain TLE1.1 in controlling stem rot disease which was carried out in a completely randomizeddesign consisting of 9 treatments and 3 replications. The treatment consistedof a combination of solid formula carriers consisting of sugarcane solid waste,tofu dreg and corncob, fungicides and controls. Each solid formula of B. cereus strain TLE1.1 was introduced into tomato seeds and seedlings. The results showed that almost all of the formulas were able to suppress stem base disease of tomato plants. The best formula that reduced stem rot in plants were the tofu dreg and tofu dreg + corncob formula.

scholarly journals Assessing the prospects of Streptomyces sp. RP1A-12 in managing groundnut stem rot disease caused by Sclerotium rolfsii Sacc

2016 ◽  
Vol 82 (2) ◽  
pp. 96-104 ◽  
Author(s):  
Simi Jacob ◽  
Ramgopal Rao Sajjalaguddam ◽  
K. Vijay Krishna Kumar ◽  
Rajeev Varshney ◽  
Hari Kishan Sudini
Keyword(s):  
Sclerotium Rolfsii ◽  
Stem Rot ◽  
Streptomyces Sp ◽  
Rot Disease

scholarly journals Comparison of Drip and Sprinkler Irrigation Systems for Applying Metam Sodium and Managing Stem Rot on Potato

Plant Disease ◽  
2002 ◽  
Vol 86 (11) ◽  
pp. 1211-1218 ◽  
Author(s):  
G. T. Browne ◽  
W. R. DeTar ◽  
B. L. Sanden ◽  
C. J. Phene
Keyword(s):  
Sclerotium Rolfsii ◽  
Sprinkler Irrigation ◽  
Stem Rot ◽  
Metam Sodium ◽  
Soil Infestation ◽  
Low Incidence ◽  
Rot Disease ◽  
Subsurface Drip ◽  
Soil Subsurface ◽  
Flat Ground

Drip and sprinkler systems were compared for effectiveness as preplant metam sodium chemigation systems and conduciveness to late-season development of stem rot disease on potato. Sclerotia of Sclerotium rolfsii were used in a bioassay to test efficacy of metam sodium treatments. Drip application of metam sodium (532 liters/ha, 32.8% a.i.) through lines at 7 cm of depth in preformed beds (depths from bed top unless stated otherwise) killed all test sclerotia at 15-, 30-, or 46-cm depths. Drip application of the metam sodium through drip lines at 41 or 46 cm of depth resulted in 0 to 17 or 68 to 80% survival, respectively, of test sclerotia at 15 cm of depth; but all the sclerotia at 30 or 46 cm of depth were killed. Compared with the drip applications, sprinkler chemigation with metam sodium generally treated beds less effectively (8 to 100% of sclerotia survived at 15 cm, 62 to 100% at 30 or 46 cm). On flat ground, drip and sprinkler chemigation (metam sodium, 560 liters/ha) performed equally (4, 37, and 77% survival at 15-, 45-, and 75-cm depths, respectively). After potato planting and artificial soil infestation with S. rolfsii (5 to 6 weeks before harvest), subsurface drip-irrigated plots (line depth of 41 or 46 cm) had lower incidence of stem rot disease at harvest (13 to 23% on tubers) than that in sprinkler plots (56 to 62%). The low incidence of disease was associated with relatively dry surface soil. Subsurface drip chemigation with metam sodium in preformed plant beds does not consistently eradicate S. rolfsii sclerotia near the upper bed surface but, in an arid climate, it is less conducive than sprinkler irrigation to development of stem rot disease of potato.

scholarly journals Sclerotium rolfsii, Penyebab Penyakit Busuk Pangkal Batang pada Hippeastrum sp.

2019 ◽  
Vol 15 (2) ◽  
pp. 53-58
Author(s):  
Antok Wahyu Sektiono ◽  
Syamsuddin Djauhari ◽  
Putri Devinta Pertiwi
Keyword(s):  
Host Range ◽  
Root Rot ◽  
Agar Medium ◽  
Sclerotium Rolfsii ◽  
Morphological Characteristics ◽  
Stem Rot ◽  
Potato Dextrose Agar Medium ◽  
White Mycelium ◽  
Rot Disease ◽  
Range Test

Sclerotium rolfsii, a the Causal Agent of Stem Rot Disease on Hippeastrum sp.Symptoms of stem rot that cause Hippeastrum sp. or red lily wither, leaves turn yellow, and eventually die found at Mangliawan Village, District of Pakis, Malang - East Java. The purpose of this study was to identify the pathogens that cause root rot disease on lily plants and find out their host range. Sclerotium from the symptomatic base of the plant was isolated on potato dextrose agar medium. Fungus was identified based on the morphological characteristics of the colonies and mycelium. Host range test of pathogen was carried out by manual inoculation on Rain lily (Zephyranthes) St. Bernard's lily (Chlorophytum) and Beach Spider lily (Hymenocallis). The results of the identification showed that the fungus had white mycelium and formed sclerotium. Sclerotium is irregularly rounded, white when young, and dark brown when ripe, and forms 10 days after incubation. In hyphae, there are branching, septa, and clam connections. Based on the morphological characteristics of the disease the fungus was identified as Sclerotium rolfsii. In the host range test, the fungus was able to infect rain lilies and paris lilies, but not in spider lilies. This is the first report of S. rolfsii infection in lily in Indonesia.

Development of Stem Rot (Caused by Sclerotium rolfsii) in Peanut in Alabama

2003 ◽  
Vol 30 (2) ◽  
pp. 120-128 ◽  
Author(s):  
K. L. Bowen
Keyword(s):  
Soil Moisture ◽  
Sclerotium Rolfsii ◽  
Field Capacity ◽  
Weather Conditions ◽  
Stem Rot ◽  
Southern Blight ◽  
Cumulative Stress ◽  
Disease Progress ◽  
Cropping Seasons ◽  
Rot Disease

Abstract Stem rot, or southern blight, caused by the fungus Sclerotium rolfsii, is an important disease of peanut in the southeastern U.S. Incidence and apparent onset of stem rot varies from year to year depending on planting date, weather conditions, and other factors. In order to more fully understand the effects of weather on stem rot, disease progress was evaluated through the 1995, 1996, and 1997 cropping seasons. Evidence of S. rolfsii was observed on peanut roots and crowns as early as 20 d after planting (DAP), which was 1 to several wk before stem rot lesions or plant wilt were observed. Disease measures tended to be lower in 1997 than in preceding years even though more rainfall and more consistent soil moisture were noted in 1997. Soil moisture was frequently in excess of field capacity from rainfall and may have contributed to suppressing stem rot development in 1997, perhaps by limiting oxygen availability. In 1995 and 1996, rainfall patterns were more normal and irrigation contributed to greater stem rot incidence. However, low moisture, specifically 7 d with < 1.8 cm rain, was associated with increases in numbers of dead plants, perhaps due to cumulative stress from disease and lack of moisture. Areas under disease progress curves for stem rot tended to be greater for early plantings compared to late plantings. However, by ca. 118 DAP, early plantings did not consistently have a greater incidence of dead plants, probably due to environmental conditions immediately preceding that date.

scholarly journals Culture-dependent analysis of seed bacterial endophyte, Pseudomonas spp. EGN 1 against the stem rot disease (Sclerotium rolfsii Sacc.) in groundnut

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
T. Archana ◽  
L. Rajendran ◽  
S. K. Manoranjitham ◽  
V. P. Santhana Krishnan ◽  
M. Paramasivan ◽  
...  
Keyword(s):  
Tamil Nadu ◽  
Vaccenic Acid ◽  
Sclerotium Rolfsii ◽  
Stem Rot ◽  
Main Body ◽  
Dual Culture ◽  
Filter Paper Disc ◽  
Bacterial Endophyte ◽  
Rot Disease ◽  
Culture Dependent

Abstract Background Groundnut, Arachis hypogaea L., crop is an important oil seed crop in India and is prone to attack by numerous fungal and viral diseases, among the soil-borne diseases, stem rot caused by Sclerotium rolfsii Sacc. is economically important. Main body of abstract Incidence (86.6%) of the stem rot disease was recorded in Coimbatore district, Tamil Nadu, India, during the roving survey. For its management, an attempt was made to isolate bacterial endophyte from peanut seeds of different groundnut cultivars by culture-dependent analysis. Totally, 16 bacterial endophytes (endophytes groundnut (EGN) 1 to EGN 16 along with standards TNAU-Pf1 and EPC 5) were obtained and characterized through morphological, biochemical and molecular studies and also phytostimulation activities were performed. Among the isolates, EGN 1 and EGN 4 showed positive results for indole acetic acid (IAA), siderophore, phosphate solubilization and protease tests in vitro. The dual culture analysis showed inhibition rates of 60.1% (dual plate assay), 68.23% (filter paper disc assay) and 100% (triangle method of streaking and culture filtrate assay) for EGN 1 against S. rolfsii. Further, the crude metabolite assay showed 97.7% inhibition in EGN 1, followed by 87.7% in EGN 4. The roll towel study showed a high vigour index of 4286.7 in EGN 1; hence, this isolate was chosen. Further, thin-layer chromatography (TLC) analysis showed various bands at 0.72 Rf, whereas GC-MS analysis indicated the prominent peaks of hexadecanoic acid and cis-vaccenic acid that may responsible for antifungal activity. In a molecular approach, the genomic DNA of EGN 1 strain was used to amplify a 1200 bp PCR-fragment and sequenced. Short conclusion The overall outcome of this study showed that Pseudomonas spp. EGN 1 had a great potential as a bio-stimulant and biocontrol agent to manage effectively the stem rot in peanut.

scholarly journals Induksi Ketahanan Tanaman Kedelai (Glycine max (L.) Merril) dengan Cendawan Endofit Trichoderma harzianum dan Beauveria bassiana untuk Menekan Penyakit Busuk Pangkal Batang (Sclerotium rolfsii)

2020 ◽  
Vol 3 (1) ◽  
pp. 6
Author(s):  
Wildatun Munawara ◽  
Nanang Tri Haryadi
Keyword(s):  
Beauveria Bassiana ◽  
Trichoderma Harzianum ◽  
Sclerotium Rolfsii ◽  
Stem Rot ◽  
Control Treatment ◽  
Soybean Crop ◽  
Combined Application ◽  
Crop Resistance ◽  
Randomized Design ◽  
Rot Disease

Sclerotium rolfsii is a pathogen that causes stem rot disease that causes a decrease in soybean production. This stem rot disease can cause damage to all parts of the plant both in the vegetative and generative phases. Proper control is needed to reduce stem rot disease by induction of plant resistance using endophytic fungi such as Trichoderma harzianum and Beauveria bassiana. This experiment used a Completely Randomized Design (CRD) with 4 treatments that were repeated 5 times, namely P1 = control, P2 = T. harzianum application + S. rolfsii inoculation, P3 = B. bassiana application + S. rolfsii inoculation, and P4 = Combined application (T. harzianum + B. Bassiana) + S. rolfsii inoculation. Based on research that has been done shows that the incubation period and phenol analysis in soybean plants did not significantly affect soybean crop resistance while in the incidence of disease and disease severity showed a very significant effect on soybean crop resistance. In this study the plants that were applied with T. harzianum and B. bassiana were included in the category of resistant to S. rolfsii pathogens, while the control treatment was included in the susceptible category to S. rolfsii pathogens.

Sign in / Sign up

Export Citation Format

Share Document