Induksi Ketahanan Tanaman Kedelai (Glycine max (L.) Merril) dengan Cendawan Endofit Trichoderma harzianum dan Beauveria bassiana untuk Menekan Penyakit Busuk Pangkal Batang (Sclerotium rolfsii)

Sclerotium rolfsii is a pathogen that causes stem rot disease that causes a decrease in soybean production. This stem rot disease can cause damage to all parts of the plant both in the vegetative and generative phases. Proper control is needed to reduce stem rot disease by induction of plant resistance using endophytic fungi such as Trichoderma harzianum and Beauveria bassiana. This experiment used a Completely Randomized Design (CRD) with 4 treatments that were repeated 5 times, namely P1 = control, P2 = T. harzianum application + S. rolfsii inoculation, P3 = B. bassiana application + S. rolfsii inoculation, and P4 = Combined application (T. harzianum + B. Bassiana) + S. rolfsii inoculation. Based on research that has been done shows that the incubation period and phenol analysis in soybean plants did not significantly affect soybean crop resistance while in the incidence of disease and disease severity showed a very significant effect on soybean crop resistance. In this study the plants that were applied with T. harzianum and B. bassiana were included in the category of resistant to S. rolfsii pathogens, while the control treatment was included in the susceptible category to S. rolfsii pathogens.

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Evaluation of plant extracts, fungicides, bio-agent and soil amendments against foot and root rot disease of betelvine caused by Sclerotium rolfsii under field condition

Bangladesh Journal of Agricultural Research ◽

10.3329/bjar.v44i4.45726 ◽

2020 ◽

Vol 44 (4) ◽

pp. 669-677

Author(s):

MH Rahman ◽

MR Islam ◽

FM Aminuzzaman ◽

A Latif ◽

S Nahar

Keyword(s):

Trichoderma Harzianum ◽

Root Rot ◽

Untreated Control ◽

Disease Incidence ◽

Sclerotium Rolfsii ◽

Control Treatment ◽

Piper Betle ◽

Root Rot Disease ◽

Clove Extract ◽

Rot Disease

The studies were carried out on the management of foot and root rot disease caused by Sclerotium rolfsii Sacc. of betelvine (Piper betle L.) during April, 2017 to April, 2018 in a betelvine orchard (baroj) in the experimental farm of Sher-e-Bangla Agricultural University, Dhaka. There were 10 treatments, namely Provax 200, Tilt 250 EC, Score 250 EC, Pencozeb 80 WP, Garlic clove extract, Allamanda leaf extract, Poultry waste, Vermi-compost, Trichoderma harzianum and Control. The in-vivo evatuation, plant was inoculated by S. rolfsii after six month of plantation. The evaluation revealed that the lowest disease incidence of 8.33% was found in Provax 200 whereas the highest disease incidence of 91.67 was recorded under the untreated control treatment. The maximum of 90.91% reduction in disease incidence over control was found under Provax 200, which was significantly higher compared to all other treatments. Among the treatments, Provax 200 was noted as the most effective fungicide followed by Score 250 EC. The highest yield (7.57 t/ha) at 120 days after inoculation was found in case of Provax 200 which was 330.1% increased over untreated control. Stem and soil treated with Provax 200 including spraying at 3 days after inoculation, then sprayed at 7 days’ intervals minimized disease incidence and increased yield. Among the eco-friendly approach, Garlic clove extract, Trichoderma harzianum and soil amended with Vermi-compost also showed better performance in controlling foot and root rot disease of betelvine as compared to control. Bangladesh J. Agril. Res. 44(4): 669-677, December 2019

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EFFECT OF INDIVIDUAL AND COMBINED APPLICATION OF Trichoderma sp. AND VERMICOMPOST ON THE MANAGEMENT OF Sclerotium rolfsii AND GROWTH OF CHILLI UNDER PEATLANDS AGRO-CLIMATIC CONDITIONS

Journal of Experimental Biology and Agricultural Sciences ◽

10.18006/2021.9(4).445.456 ◽

2021 ◽

Vol 9 (4) ◽

pp. 445-456

Author(s):

Adi Jaya ◽

◽

Bambang S. Lautt ◽

Emmy Uthanya Antang ◽

Lilies Supriati ◽

...

Keyword(s):

Growth Parameters ◽

Peat Soil ◽

Sclerotium Rolfsii ◽

Climatic Conditions ◽

Growth And Yield ◽

Stem Rot ◽

Combined Application ◽

Trichoderma Sp ◽

Biocontrol Potential ◽

Rot Disease

This study was conducted to evaluate the biocontrol potential of Trichoderma sp., and vermicompost on the management of Sclerotium rolfsii and chilli plant growth under peatlands agro-climatic conditions. The research was conducted under the laboratory as well field condition of Palangka Raya University in a completely randomized factorial design. As a treatment, four doses of Trichoderma sp. i.e. 0, 5, 10, and 15 g/box, and three doses of Vermicompost i.e. 0, 100, and 200g/ box was used individually and in combination and each treatment have three replications. Three kilograms of sterilized peat soil planting media were placed in the 36 plastic boxes and Trichoderma sp., and vermicompost was applied 1 week before the chilli seeds are planted. After 10 weeks of plantations, observations related to the disease intensity and chilli growth and yield were recorded. The results of the study revealed a synergistic effect of Trichoderma sp. and vermicompost combination in controlling the stem rot disease caused by S. rolfsii. Individual and combined application of Trichoderma and vermicompost had an effective range of 73.33-100% over the control. Further, among the various tested treatments, a combination of Trichoderma sp @ 5g/box with vermicompost @ 200g/box and individual application of Trichoderma sp @ 10g/box were found superior in the management of stem rot disease. In the case of chilli growth parameters, the results of the study suggested that the application of vermicompost had a significant effect on the chilli plant's growth and yield, while the application of Trichoderma sp. significantly affects the chilli plants number of leaves.

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Biological Control Stem Rot Diseases (Sclerotium Rolfsii) on Peanut (Arachis hypogaea L.) using Arbuscular Mycorrhizal Fungi (AMF) Indigenous

Akta Agrosia ◽

10.31186/aa.24.1.25-31 ◽

2021 ◽

Vol 24 (1) ◽

pp. 25-31

Author(s):

Fradilla Swandi ◽

Eri Sulyanti ◽

Darnetty Darnetty

Keyword(s):

Arbuscular Mycorrhizal Fungi ◽

Mycorrhizal Fungi ◽

Plant Pathogens ◽

Sclerotium Rolfsii ◽

Arbuscular Mycorrhizal ◽

Stem Rot ◽

Significance Level ◽

Randomized Design ◽

Rot Disease ◽

Glomus Sp

Arbuscular Mycorrhizal Fungi (AMF) are known to have potential as biological agents controlling plant pathogens. This study aims to obtain indigenous AMF isolates that can suppress the attack of Sclerotium rolfsii which causes stem rot disease in peanut. The method used is an experimental method with a Completely Randomized Design with 5 treatments, namely A : AMF Glomus sp-3 + S. rolfsii; B: AMF Acaulospora sp + S. rolfsii; C: AMF Gigaspora sp + S. rolfsii; D: Combined AMF Glomus sp-3, Acaulospora sp, and Gigaspora sp + S. rolfsii; E: Without AMF + S. rolfsii (Control). Each treatment was repeated 5 times. The data were analyzed using Analysis of Variance (ANOVA) using the Statistix 8 program and the Least Significance Different (LSD) test at a 5% significance level. The results showed that the isolates of Acaulospora sp and Gigaspora sp were able to increase the resistance of peanut plants to stem rot disease (suppressing the incidence and severity of the disease) reaching 100%. Keywords: Arbuscular Mycorrhizal Fungi, indigenous, salicylic acid, Sclerotium rolfsii.

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ASOSIASI CENDAWAN ANTAGONIS TRICHODERMA HARZIANUM RIFAI DAN CENDAWAN MIKORIZA ARBUSKULAR UNTUK MENGENDALIKAN PENYAKIT BUSUK PANGKAL BATANG PADA KEDELAI

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.214160-169 ◽

2014 ◽

Vol 14 (2) ◽

pp. 160-169

Author(s):

Latifah Latifah ◽

Hendrival Hendrival ◽

Mihram Mihram

Keyword(s):

Arbuscular Mycorrhizal Fungi ◽

Trichoderma Harzianum ◽

Mycorrhizal Fungi ◽

Block Design ◽

Sclerotium Rolfsii ◽

Arbuscular Mycorrhizal ◽

Positive Control ◽

Negative Control ◽

Stem Rot ◽

Rot Disease

Association of antagonistic fungi Trichoderma harzianum Rifai and arbuscular mycorrhizal fungi for controlling the stem rot disease on soybean. The research objective was to study effect of application Trichoderma harzianum and arbuscular mycorrhizal fungi (AMF) in controling stem rot disease caused by Sclerotium rolfsii Sacc on soybean. The research conducted in a Randomized Block Design (RBD) with five treatment levels: (1) without T. harzianum, without AMF, and without S. rolfsii [negative control (C-)], (2) without T. harzianum, without AMF, and S. rolfsii [positive control (C +)], (3) T. harzianum + S. rolfsii; (4) AMF + S. rolfsii, and (5) T. harzianum + AMF + S. rolfsii. Parameters observed were basal stem rot disease development and yield components. The results showed that the application of a mixture of T. harzianum and AMF caused a longer disease incubation period (8.29 days) and the severity of stem base rot disease was 11.67% number of pods per plant (62.53 pods), the number of seeds per plant (225.05 seeds), and the weight of seeds per plant (27.73 g) were higher than that of the application of T. harzianum and AMF separately.

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Assessing the prospects of Streptomyces sp. RP1A-12 in managing groundnut stem rot disease caused by Sclerotium rolfsii Sacc

Journal of General Plant Pathology ◽

10.1007/s10327-016-0644-0 ◽

2016 ◽

Vol 82 (2) ◽

pp. 96-104 ◽

Cited By ~ 9

Author(s):

Simi Jacob ◽

Ramgopal Rao Sajjalaguddam ◽

K. Vijay Krishna Kumar ◽

Rajeev Varshney ◽

Hari Kishan Sudini

Keyword(s):

Sclerotium Rolfsii ◽

Stem Rot ◽

Streptomyces Sp ◽

Rot Disease

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Comparison of Drip and Sprinkler Irrigation Systems for Applying Metam Sodium and Managing Stem Rot on Potato

Plant Disease ◽

10.1094/pdis.2002.86.11.1211 ◽

2002 ◽

Vol 86 (11) ◽

pp. 1211-1218 ◽

Cited By ~ 5

Author(s):

G. T. Browne ◽

W. R. DeTar ◽

B. L. Sanden ◽

C. J. Phene

Keyword(s):

Sclerotium Rolfsii ◽

Sprinkler Irrigation ◽

Stem Rot ◽

Metam Sodium ◽

Soil Infestation ◽

Low Incidence ◽

Rot Disease ◽

Subsurface Drip ◽

Soil Subsurface ◽

Flat Ground

Drip and sprinkler systems were compared for effectiveness as preplant metam sodium chemigation systems and conduciveness to late-season development of stem rot disease on potato. Sclerotia of Sclerotium rolfsii were used in a bioassay to test efficacy of metam sodium treatments. Drip application of metam sodium (532 liters/ha, 32.8% a.i.) through lines at 7 cm of depth in preformed beds (depths from bed top unless stated otherwise) killed all test sclerotia at 15-, 30-, or 46-cm depths. Drip application of the metam sodium through drip lines at 41 or 46 cm of depth resulted in 0 to 17 or 68 to 80% survival, respectively, of test sclerotia at 15 cm of depth; but all the sclerotia at 30 or 46 cm of depth were killed. Compared with the drip applications, sprinkler chemigation with metam sodium generally treated beds less effectively (8 to 100% of sclerotia survived at 15 cm, 62 to 100% at 30 or 46 cm). On flat ground, drip and sprinkler chemigation (metam sodium, 560 liters/ha) performed equally (4, 37, and 77% survival at 15-, 45-, and 75-cm depths, respectively). After potato planting and artificial soil infestation with S. rolfsii (5 to 6 weeks before harvest), subsurface drip-irrigated plots (line depth of 41 or 46 cm) had lower incidence of stem rot disease at harvest (13 to 23% on tubers) than that in sprinkler plots (56 to 62%). The low incidence of disease was associated with relatively dry surface soil. Subsurface drip chemigation with metam sodium in preformed plant beds does not consistently eradicate S. rolfsii sclerotia near the upper bed surface but, in an arid climate, it is less conducive than sprinkler irrigation to development of stem rot disease of potato.

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Sclerotium rolfsii, Penyebab Penyakit Busuk Pangkal Batang pada Hippeastrum sp.

Jurnal Fitopatologi Indonesia ◽

10.14692/jfi.15.2.53-58 ◽

2019 ◽

Vol 15 (2) ◽

pp. 53-58

Author(s):

Antok Wahyu Sektiono ◽

Syamsuddin Djauhari ◽

Putri Devinta Pertiwi

Keyword(s):

Host Range ◽

Root Rot ◽

Agar Medium ◽

Sclerotium Rolfsii ◽

Morphological Characteristics ◽

Stem Rot ◽

Potato Dextrose Agar Medium ◽

White Mycelium ◽

Rot Disease ◽

Range Test

Sclerotium rolfsii, a the Causal Agent of Stem Rot Disease on Hippeastrum sp.Symptoms of stem rot that cause Hippeastrum sp. or red lily wither, leaves turn yellow, and eventually die found at Mangliawan Village, District of Pakis, Malang - East Java. The purpose of this study was to identify the pathogens that cause root rot disease on lily plants and find out their host range. Sclerotium from the symptomatic base of the plant was isolated on potato dextrose agar medium. Fungus was identified based on the morphological characteristics of the colonies and mycelium. Host range test of pathogen was carried out by manual inoculation on Rain lily (Zephyranthes) St. Bernard's lily (Chlorophytum) and Beach Spider lily (Hymenocallis). The results of the identification showed that the fungus had white mycelium and formed sclerotium. Sclerotium is irregularly rounded, white when young, and dark brown when ripe, and forms 10 days after incubation. In hyphae, there are branching, septa, and clam connections. Based on the morphological characteristics of the disease the fungus was identified as Sclerotium rolfsii. In the host range test, the fungus was able to infect rain lilies and paris lilies, but not in spider lilies. This is the first report of S. rolfsii infection in lily in Indonesia.

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