scholarly journals Notes from the Field: Enteroinvasive Escherichia coli Outbreak Associated with a Potluck Party — North Carolina, June–July 2018

2019 ◽  
Vol 68 (7) ◽  
pp. 183-184 ◽  
Author(s):  
Carolyn T.A. Herzig ◽  
Aaron T. Fleischauer ◽  
Brian Lackey ◽  
Nicole Lee ◽  
Thomas Lawson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
North Carolina ◽  
June July

Sea Turtle Bycatch in the Large-Mesh Gillnet Flounder Fishery in Carteret County, North Carolina, USA, June-November 2009

2016 ◽  
Vol 132 (1-2) ◽  
pp. 10-24 ◽  
Author(s):  
Barbie L. Byrd ◽  
Lisa R. Goshe ◽  
Trip Kolkmeyer ◽  
Aleta A. Hohn
Keyword(s):  
North Carolina ◽  
Endangered Species Act ◽  
Chelonia Mydas ◽  
Sea Turtle ◽  
Mitigation Measures ◽  
String Length ◽  
Lepidochelys Kempii ◽  
Turtle Species ◽  
Spatio Temporal ◽  
June July

Abstract Sea turtle bycatch has been documented in the large-mesh gillnet fishery that targets flounder in estuarine waters of North Carolina (NC). However, only portions of the fishery operated under Endangered Species Act Incidental Take Permits and had regular observer coverage to determine the occurrence and extent of sea turtle bycatch. From June through November 2009, an Alternative Platform Observer Program (APOP) was initiated in southeastern Carteret County, NC, to document turtle entanglements. Observers covered 1.6% of the total number of large-mesh gillnet trips reported (1.1% of landings) and documented turtle bycatch (n = 22) on 36% of the observed trips (12 of 33). Most turtles were recovered alive (n = 15), and all interactions occurred in June, July, and August. Bycaught sea turtle species included 12 greens (Chelonia mydas), 5 Kemp’s ridleys (Lepidochelys kempii), and 5 loggerheads (Caretta caretta). Hauls with bycaught turtles in June had a significantly greater mean string length than those without bycatch (P = 0.02), but despite the institution of regulations limiting string length, no difference was found in mean string length overall before (June) and after (July-November) regulations went into effect. Documented turtle bycatch in this area supports the need for observer coverage across the entire spatio-temporal scope of the fishery at levels necessary for robust bycatch estimates. Representative observer data across longer time series can inform managers where and when bycatch risks are greatest and help in developing mitigation measures that decrease bycatch risk while reducing negative economic impacts on the fishers.

scholarly journals Whole-Genome Sequencing of Multidrug-Resistant Escherichia coli Isolates From Community Hospitals in North Carolina, 2010–2015

2016 ◽  
Vol 3 (suppl_1) ◽  
Author(s):  
Hajime Kanamori ◽  
Christian Parobek ◽  
Jonathan Juliano ◽  
James R. Johnson ◽  
Brian D. Johnston ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
North Carolina ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Multidrug Resistant ◽  
Whole Genome ◽  
Community Hospitals

Assessment of Microbiological Safety and Quality of Marinades Used To Treat Beef and That Were Collected over a 12-Month Period from Specialty Retailers Near Raleigh, North Carolina

2018 ◽  
Vol 81 (3) ◽  
pp. 490-496 ◽  
Author(s):  
Yangjin Jung ◽  
Christopher L. Rupert ◽  
Benjamin Chapman ◽  
Anna C. S. Porto Fett ◽  
John B. Luchansky
Keyword(s):  
Escherichia Coli ◽  
North Carolina ◽  
Real Time ◽  
Shiga Toxin ◽  
Real Time Pcr ◽  
Plate Count ◽  
Pcr Assay ◽  
E Coli ◽  
Aerobic Plate Count

ABSTRACT In total, 115 marinade samples (58 fresh marinades and 57 spent marinades) were collected over 12 months from specialty retailers (four individual stores) near Raleigh, NC. These marinades were screened for total mesophilic aerobic plate count (M-APC), total psychrotrophic aerobic plate count (P-APC), and Enterobacteriaceae. These marinades were also screened for the seven regulated serogroups of Shiga toxin–producing Escherichia coli. Stores A and B used immersion to marinade raw beef cuts, whereas stores C-1 and C-2 used vacuum tumbling. In general, marinade temperatures at the stores ranged from 1.8 to 6.6°C, and beef cuts were marinated from a few minutes to up to 3 days. Regardless of the process used to marinade meat, levels of M-APC and P-APC in fresh marinades ranged from 3.4 to 4.7 and 1.4 to 1.8 log CFU/mL, respectively, whereas Enterobacteriaceae were not detected in any fresh marinades, even after enrichment. However, levels of M-APC, P-APC, and Enterobacteriaceae in spent marinades collected from stores C-1 and C-2 (ca. 3.6 to 7.1 log CFU/mL) were significantly higher (P < 0.05) compared with levels of these same types of bacteria enumerated from spent marinades collected at stores A and B (ca. ≤0.7 to 4.9 log CFU/mL). None of the 115 marinade samples tested positive for Shiga toxin–producing E. coli by using a BAX system real-time PCR assay. No significant (P > 0.05) association was observed between microbial levels (i.e., M-APC, P-APC, and Enterobacteriaceae) and the temperature or duration of the marination process. Levels of M-APC, P-APC, and Enterobacteriaceae in spent marinades were significantly affected by the marination method (P < 0.05), with levels, in general, being higher in marinades used for tumbling. Thus, retailers must continue to keep marinade solutions and meat at a safe temperature (i.e., ≤4°C) and to properly and frequently sanitize the equipment and environment in both the processing area and deli case.

scholarly journals Qualidade Hídrica da Cachoeira do Rio São Tomás, no Município de Rio Verde, Goiás, Brasil

2020 ◽  
Vol 13 (1) ◽  
pp. 377
Author(s):  
Maria Antonia Balbino Pereira ◽  
Wellmo Dos Santos Alves ◽  
Lucas Duarte De Oliveira ◽  
Wilker Alves Morais ◽  
Luanna Oliveira Lima ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Trophic State Index ◽  
Drought Period ◽  
Rainy Period ◽  
E Coli ◽  
Inappropriate Use ◽  
Negative Results ◽  
June July

A água é um recurso fundamental para a vida na Terra. Analisar e monitorar esse bem é uma das formas de auxiliar na conservação da sua qualidade. Este trabalho teve como objetivo analisar a qualidade da água da Cachoeira do Rio São Tomás por meio de variáveis de qualidade da água, Índice de Estado Trófico (IET) e balneabilidade. Foram realizadas sete campanhas, quatro no período chuvoso (novembro e dezembro de 2017 e janeiro e fevereiro de 2018) e três no período de estiagem (junho, julho e agosto de 2018). As variáveis físico-químicas analisadas foram temperatura (T), condutividade elétrica (CE), sólidos totais dissolvidos (STD), potencial hidrogeniônico (pH), turbidez, fósforo total (PT) e nitrato, sendo os valores de referência os estabelecidos pela Resolução CONAMA 357/05 e observados na literatura. O estudo do IET foi realizado considerando os resultados obtidos para PT. Para balneabilidade, a variável obtida foi Escherichia coli (E. coli) e os resultados comparados com os limites descritos na Resolução CONAMA 274/00. A precipitação foi analisada para melhor entender os resultados. A Turbidez e o PT estiveram em inconformidade com os limites determinados na Resolução CONAMA 357/05. Para IET a água foi classificada nos mais altos graus de trofia (supereutrófico e hipereutrófico). Em relação a balneabilidade, a água foi classificada como impropria para banho em três campanhas e para a média geral. Os resultados negativos podem ter relação com o uso e ocupação do solo inadequados associados à precipitação, o que favorece o carreamento de resíduos para o corpo d’água. Water Quality of Cachoeira do Rio São Tomás, in the Municipality of Rio Verde, Goiás, BrazilA B S T R A C TThe water is a primordial resource for life on Earth. Analyzing and monitoring this asset is one of the ways to assist on the preservation of its quality. This paper had as objective to analyze the quality of the water of Cachoeira do Rio São Tomás through variables of water quality, Trophic State Index (TSI) and bathing. Seven campaigns were accomplished, four during the rainy period (November and December of 2017 and January and February of 2018) and three during the drought period (June, July and August of 2018). The physical-chemical variables analyzed were temperature (T), electric conductivity (EC), total dissolved solids (TDS), hydrogen potential (pH), turbidity, total phosphorus (TP) and nitrate, considering the reference values the ones stablished by CONAMA Resolution 357/05 and observed in literature. The study of TSI was accomplished considering the obtained results for TP. For bathing, the obtained variable was Escherichia coli (E. coli) and the results compared to the described limit of CONAMA Resolution 274/00.  The rainfall was analyzed for better understanding of the results. The Turbidity and TP were in nonconformity with the limits determined by the CONAMA Resolution 357/05. For TSI the water was classified in the highest degrees of trophic (supereutrophic and hypereutrophic). In relation to bathing, the water was classified as inappropriate for bathing in three campaigns and for the general average. The negative results can be related to the inappropriate use and coverage of the soil associated to rainfall, which favors the solids transport to the water body.Keywords: anthropic action, bathing, eutrophication, water resources, subsidy for water management.

scholarly journals COVID-19 Contact Tracing in Two Counties — North Carolina, June–July 2020

2020 ◽  
Vol 69 (38) ◽  
pp. 1360-1363 ◽  
Author(s):  
R. Ryan Lash ◽  
Catherine V. Donovan ◽  
Aaron T. Fleischauer ◽  
Zack S. Moore ◽  
Gibbie Harris ◽  
...  
Keyword(s):  
North Carolina ◽  
Contact Tracing ◽  
June July

scholarly journals Widespread Dissemination of CTX-M-15 Genotype Extended-Spectrum-β-Lactamase-Producing Enterobacteriaceae among Patients Presenting to Community Hospitals in the Southeastern United States

2013 ◽  
Vol 58 (2) ◽  
pp. 1200-1202 ◽  
Author(s):  
Luke F. Chen ◽  
Joshua T. Freeman ◽  
Brad Nicholson ◽  
Anna Keiger ◽  
Sarah Lancaster ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
North Carolina ◽  
Southeastern United States ◽  
Sequence Type ◽  
Community Hospitals ◽  
Content Type ◽  
E Coli ◽  
Extended Spectrum ◽  
Widespread Dissemination ◽  
M 14

ABSTRACTExtended-spectrum-β-lactamase (ESBL)-producing organisms are increasingly prevalent. We determined the characteristics of 66 consecutive ESBL-producing isolates from six community hospitals in North Carolina and Virginia from 2010 to 2012. Fifty-three (80%) ESBL-producing isolates contained CTX-M enzymes; CTX-M-15 was found in 68% ofEscherichia coliand 73% ofKlebsiellaisolates. Sequence type 131 (ST131) was the commonest type ofE. coli, accounting for 48% of CTX-M-15-producing and 66% of CTX-M-14-producing isolates. In conclusion, the CTX-M genotype and ST131E. coliwere common among ESBL isolates from U.S. community hospitals.

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