Light Quality Influences Indigo Precursors Production and Seed Germination in Isatis tinctoria L. and Isatis indigotica Fort¶

Environmental factors such as the existence of exogenous sugar and light quality during seed germination may affect the growth and quality of seedlings. This experiment was aimed to evaluate the effect of glucose and light quality on seed germination percentage, vitamine E and chlorophyll content in green bean sprout (Vigna radiata (L.) Wilcz). The experiment design used was 5x5 factorial. The main factor was glucose concentration which consisted of 5 levels (0%, 0.5%, 1%, 2.5% or 5 %). The second factor was variation of light quality which consists of 3 different light spectrums namely blue, red or green as well as sunlight and dark condition. Seeds were selected, sterilized and germinated in a petridish containing various level of glucose. Fourty seeds were germinated in each petridish and 5 replicates were used per treatment. Petridish with seeds in it were then placed in a growth chamber having different light quality (blue, red or green), under dark condition or light condition. Growth parameters observed were germination percentage and height of bean sprout. In addition, vitamine E and chlorophyll conten were determined using spectrophotometer. Data were analyzed by using analysis of variance (ANAVA) and followed by Duncan’s Multiple Range Test (DMRT) at significant level of 5%. The results showed that increasing glucose content caused reduction in the germination percentage and height of bean sprouts whereas green and blue light also reduced seed germination. The vitamine E content in green bean sprout grown under red and blue light was relatively similar compared to those grown under sunlight or dark condition, however, green light decreased the vitamine E content in green bean sprout. In addition, the existence of glucose and light quality influenced chlorophyll content in green bean sprout. Keywords : glucose, light quality, green bean, vitamine E, chlorophyll content.

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Experimental seed germination for ex situ conservation of Mexican Podostemaceae

Botanical Sciences ◽

10.17129/botsci.2232 ◽

2019 ◽

Vol 97 (3) ◽

pp. 413 ◽

Cited By ~ 1

Author(s):

Valeria Flores-Enríquez ◽

Guillermo Castillo ◽

Margarita Collazo-Ortega

Keyword(s):

Seed Germination ◽

Storage Time ◽

Light Quality ◽

Germplasm Conservation ◽

Seed Storage ◽

Germination Percentage ◽

Seed Banks ◽

Ex Situ ◽

Effect Of Temperature ◽

Collection Site

Background: Podostemaceae are extremely susceptible to local extinction by habitat loss. Since ~70 % of the river systems in Mexico show some degree of water contamination, it is relevant to generate information about seed storage and germination behavior to design germplasm conservation strategies (e.g., ex situ seed banks) of Mexican podostemads.Hypotheses: Seed germination decreases as seed storage time increases. Further, light quality, temperature and collection site influence similarly the germination response of both species.Studied species: Marathrum foeniculaceum Humb. & Bonpl., Noveloa coulteriana (Tul.) C. PhilbrickStudy site and years of study: 13 different seeds collections (1996-2013), at four locations in the rivers Horcones and Arroyo del Rincon Jalisco, México. Methods: A germination chamber experiment was performed to evaluate the effect of temperature, light quality and storage time on the germination of both species.Results: Seeds lose viability after nine years of storage. In both species, the time to reach the highest Accumulated Germination Percentage (AGP) was faster in seeds of one-two yr compared to seeds of six-seven yr. N. coulteriana have significant differences in Final Germination Percentage (FGP) between collection rivers. Storage time affects AGP of N. coulteriana more than in M. foeniculaceum.Conclusions: Both species can form ex situ seed banks up to 8 yr age in paper bags storage. N. coulteriana is more susceptible to variation in storage conditions compared to M. foeniculaceum. Collection site affect seed germination after storing. Long-term conventional ex situ seed germination storage is not a viable strategy to conserve germplasm of Podostemaceae.

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Effect of light quality on Tabebuia rosea seed germination

Universitas Scientiarum ◽

10.11144/javeriana.sc20-2.elqt ◽

2014 ◽

Vol 20 (2) ◽

pp. 191

Author(s):

Sofía Basto ◽

Claudia Ramírez

Keyword(s):

Seed Germination ◽

Light Quality ◽

Germination Rate ◽

Germination Capacity ◽

Germination Speed ◽

Tabebuia Rosea ◽

Germination Value ◽

Positive Effect ◽

Effect Of Light ◽

Peak Value

Tabebuia rosea germination capacity and rate have been examined under light and dark conditions. However, seeds responses to light quality, in particular to short and mid-wavelengths, are not well known. We hypothesized that short wavelengths would have a positive effect on rosea germination. The effects of short, mid and long-wavelengths on germination capacity, rate (germination rate index, germination rate R50, a devised index of germination rate R50´ and peak value), mean daily germination and germination value were evaluated. Light quality had little effect on germination capacity; it was reduced only in darkness. Seeds under short and mid-wavelengths germinated faster (germination rate) than under any other light treatments. We concluded that althoughlt T. rosea seeds respond to all light quality treatments, the short and mid-wavelengths are the cue for increasing germination speed. For synchronizing germination, short and mid-wavelengths transmitted by red cellophane paper can be used as a pre-germinative treatment for restoration and silvicultural purposes.

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