scholarly journals Experimental seed germination for ex situ conservation of Mexican Podostemaceae

2019 ◽  
Vol 97 (3) ◽  
pp. 413 ◽  
Author(s):  
Valeria Flores-Enríquez ◽  
Guillermo Castillo ◽  
Margarita Collazo-Ortega
Keyword(s):  
Seed Germination ◽  
Storage Time ◽  
Light Quality ◽  
Germplasm Conservation ◽  
Seed Storage ◽  
Germination Percentage ◽  
Seed Banks ◽  
Ex Situ ◽  
Effect Of Temperature ◽  
Collection Site

<p><strong>Background</strong>: Podostemaceae are extremely susceptible to local extinction by habitat loss. Since ~70 % of the river systems in Mexico show some degree of water contamination, it is relevant to generate information about seed storage and germination behavior to design germplasm conservation strategies (<em>e.g</em>., <em>ex situ</em> seed banks) of Mexican podostemads.</p><p><strong>Hypotheses</strong>: Seed germination decreases as seed storage time increases. Further, light quality, temperature and collection site influence similarly the germination response of both species.</p><p><strong>Studied species</strong>: <em>Marathrum foeniculaceum</em> Humb. &amp; Bonpl., <em>Noveloa coulteriana</em> (Tul.)<em> </em>C. Philbrick</p><p><strong>Study site and years of study</strong>: 13 different seeds collections (1996-2013), at four locations in the rivers Horcones and Arroyo del Rincon Jalisco, México. </p><p><strong>Methods</strong>: A germination chamber experiment was performed to evaluate the effect of temperature, light quality and storage time on the germination of both species.</p><p><strong>Results</strong>: Seeds lose viability after nine years of storage. In both species, the time to reach the highest Accumulated Germination Percentage (AGP) was faster in seeds of one-two yr compared to seeds of six-seven yr. <em>N. coulteriana</em> have significant differences in Final Germination Percentage (FGP) between collection rivers. Storage time affects AGP of <em>N. </em>coulteriana more than in <em>M. foeniculaceum.</em></p><p><strong>Conclusions</strong>: Both species can form <em>ex situ</em> seed banks up to 8 yr age in paper bags storage. <em>N. coulteriana</em> is more susceptible to variation in storage conditions compared to <em>M. </em><em>foeniculaceum</em>. Collection site affect seed germination after storing.  Long-term conventional <em>ex situ</em> seed germination storage is not a viable strategy to conserve germplasm of Podostemaceae.</p>

scholarly journals The Morphological Features and Biology of a Relict and Endangered Woody Plant Species: Chamaedaphne calyculata (L.) Moench (Ericaceae)

Plants ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 129
Author(s):  
Anna Źróbek-Sokolnik ◽  
Piotr Dynowski ◽  
Mieczysława Aldona Fenyk
Keyword(s):  
Leaf Blade ◽  
Storage Time ◽  
Seed Storage ◽  
Germination Percentage ◽  
Ex Situ ◽  
Germination Index ◽  
Western Boundary ◽  
Biometric Analysis ◽  
Chamaedaphne Calyculata ◽  
Germination Process

Chamaedaphne calyculata (L.) Moench is a rare post-glacial relic, which reaches the south-western boundary of its European range in Poland. However, little is known about the morphology and biology of this species. In this study a biometric analysis of assimilating leaves and general morphological appearance was carried out; flowering, fruiting, and seed production in a natural site were described and the ability of seed germination was determined after varied seed storage time at 0–2, 2–4, 6–8, and 21–23 °C. A quite large intrapopulation variability was found as regards the features under analysis. The most varied features included the length of the petiole, followed by the leaf blade area, while the length to the width of the leaf blade was the least varied feature. C. calyculata flowered abundantly and about 50% developed flowers turned into fruits. On average there were 56 seeds per fruit with a predominance of mature seeds. The length of the seed storage time had a significant effect on all parameters of the germination process. The temperature at which the seeds were stored, apart from the time to maximum germination parameter, had a significant effect on other parameters of the germination process. Interactions between the seeds storage time and temperature factors were observed only for germination percentage, germination index, and germination index rate parameters. The ability of seeds to germinate, non-creation of the seed bank and other results of the research provide new information necessary for planning and carrying out conservation procedures (including active conservation in ex situ conditions).

Factors affecting red-osier dogwood seed germination

1992 ◽  
Vol 70 (5) ◽  
pp. 1012-1016 ◽  
Author(s):  
S. N. Acharya ◽  
C. B. Chu ◽  
R. Hermesh ◽  
G. B. Schaalje
Keyword(s):  
Seed Germination ◽  
Germination Percentage ◽  
Collection Site ◽  
Moisture Condition ◽  
Seed Formation ◽  
Factors Affecting ◽  
Year Effect ◽  
Wide Range ◽  
Cornus Stolonifera ◽  
Moisture Conditions

Seeds from 55 populations of native Alberta red-osier dogwood (Cornus stolonifera Michx.) were collected over 2 years (1984 and 1985) to determine the range of variability for seed germination and the effect of ecoregion and moisture conditions in the collection sites on this trait. Each year the unscarified seeds were stored at 3 °C for 30 days, stratified at 3 °C for an additional 30 days and then incubated on an 8 h light: 16 h dark cycle at a temperature of 25:10 °C (light:dark) for germination. A wide range of germination percentages was observed among populations collected in any 1 year. However, the variability was not due to the ecoregion or moisture condition of the collection site. Germination percentage of populations collected in 1984 and 1985 formed five and seven groups, respectively. A study involving eight sites that were common to 1984, 1985, and 1986 revealed a significant effect of population on seed germination. This population effect was not obscured by the combined effects of year, precipitation, temperature, and seed size. The year effect was significant only when population was used as a covariate. Precipitation during seed formation significantly influenced germination performance even after adjustments were made for population and year. Seed germination in red-osier dogwood appears to be influenced by the population from which the seeds are collected and by yearly environmental fluctuations. Therefore, germination studies in this species must include seeds from diverse populations collected over several years. Key words: red-osier dogwood, Cornus stolonifera Michx., germination, population, environment.

scholarly journals Effect of the interactions between moisture content, seed storage time and use of pesticides on seed germination, viability and viability indicators of wheat crop

2016 ◽  
Vol 4 (4) ◽  
pp. 406-413
Author(s):  
Mohammad Ali JAHANBIN ◽  
◽  
Hasan Hüseyin GEÇİT ◽  
ÜNVER İKİNCİKARAKAYA ◽  
◽  
...  
Keyword(s):  
Seed Germination ◽  
Moisture Content ◽  
Storage Time ◽  
Seed Storage ◽  
Wheat Crop

High seed viability recorded in an endangered endemic species, 'Isoplexis isabelliana' (Scrophulariaceae), after more than 30 years of storage in a conservation seed bank

2021 ◽  
Vol 42 ◽  
pp. e69341
Author(s):  
Miguel Ángel González Pérez ◽  
Nereida Cabrera-García ◽  
Isabel Cayon-Fernández
Keyword(s):  
Seed Bank ◽  
Germination Rate ◽  
Genetic Material ◽  
Natural Populations ◽  
Seed Viability ◽  
Seed Storage ◽  
Botanical Garden ◽  
Storage Conditions ◽  
Seed Banks ◽  
Ex Situ

Conservation seed banks are essential for ex-situ conservation of genetic biodiversity. These institutions are especially relevant for threatened species and play a vital role in their conservation by preserving genetic material. However, samples deposited in the seed banks must germinate when necessary to use them (i.e., recovery plans, etc.). This study uses four accessions of the endemic endangered species from Gran Canaria Island (Canary Islands), Isoplexis isabelliana (Webb & Berthel.) Masf. (Scrophulariaceae). Germination tests were carried out to measure seed viability through time and the possible impact of seed storage on their viability. These accessions have been kept in the seed bank for four months to thirty years under different storage conditions. Germination results differed for seeds after 45 days of exposition using 16 hours light and 8 hours darkness at 17 °C. Accessions kept in the seed bank, independently of storage, showed a high germination percentage (89%). Whereas the accessions with rough storage conditions showed a 0% germination rate. The results highlighted the good state of conservation of the material deposited in the Seed Bank of the Botanical Garden "Viera y Clavijo" and the reliability of the temperature and humidity conditions in which the seeds of I. isabelliana have been stored. We consider these results as momentous since several natural populations of I. isabelliana has been affected by the last forest fire on the island.

Dispersal and germination syndromes of tree seeds in a monsoonal forest in northern India

2005 ◽  
Vol 15 (1) ◽  
pp. 29-42 ◽  
Author(s):  
R.C. Thapliyal ◽  
S.S. Phartyal
Keyword(s):  
Seed Germination ◽  
Seed Storage ◽  
Germination Percentage ◽  
Cold Season ◽  
Poor Quality ◽  
Northern India ◽  
High Germination ◽  
Monsoon Forest ◽  
Mean Germination Time ◽  
Pre Treatment

This paper describes the dispersal–germination characteristics of seeds of 77 native tree species in a seasonal monsoon forest in Uttaranchal state, northern India. Results indicate that 50% of the species dispersed in the hot, dry summer months, 18% during the rainy season, 23% during the cold season and the remainder in late spring. Germination tests on fresh and laboratory-stored seeds revealed a relationship between morphological features of the fruit and both germination percentage and mean germination time (MGT). Highest mean germination (50%) was for dry-dehiscent fruits with winged wind-dispersed seeds, followed by dry-dehiscent fruits with non-winged seeds (38%) and seeds of dry-indehiscent fruits (37%). Lowest germination (29%) was for seeds from fleshy or pulpy fruits. MGT followed the reverse course. Germination data for seeds stored dry in the laboratory during one seeding cycle indicated six patterns of seed germination: (1) average germination percentage of fresh seeds lower than that of stored seeds, indicating an after-ripening requirement; (2) initial high germination percentage followed by low values, indicating a steep to moderate decline in viability following harvest; (3) no germination after 1 or 2 months of seed storage, due to complete loss of viability, indicating short seed longevity; (4) fresh seed germination in some species equalled the average germination value of stored seeds, indicating constant germination for one whole seeding cycle; (5) germination of both fresh and stored seeds remained consistently low throughout the season, indicating a requirement for some kind of pre-treatment or having poor quality of seeds; (6) initial high germination followed by decline and again increase, showing a seasonal rhythm of germination.

scholarly journals The impact of temperature patterns during storage of Scots pine and Norway spruce seeds on their germination and fungal infection rates

2021 ◽  
Vol 182 (1) ◽  
pp. 157-167
Author(s):  
M. A. Nikolaeva ◽  
E. Yu. Varentsova ◽  
G. F. Safina
Keyword(s):  
Seed Germination ◽  
Seed Viability ◽  
Seed Storage ◽  
Pathogenic Fungi ◽  
Forest Tree ◽  
Ex Situ ◽  
Seed Infection ◽  
Germination Capacity ◽  
Long Term Storage ◽  
The Impact

Relevance of the study. One of the ways to maintain the genetic diversity of forest-forming species is to preserve highquality seed material ex situ. However, the relationship between the diversity of pathogenic mycobiota and the duration and methods of forest tree seed storage remains underexplored. The results of research into this problem can be used in forest seed production and forest phytopathology.Materials and methods. For our study we used seeds ofРinus sylvestris L. and Picea abies (L.) Karst. of the orthodox type, harvested in the period of 1996–2011 and stored under different temperatures: +20°С, +4°С, –18°С, and in liquid nitrogen vapor (–182°С) since 2011. Prior to their storage, seed samples were dried to a moisture content of 4.2–4.4% and hermetically packed. Seed germination was tested before and after three, five and eight years of storage, following GOST 13056.6-97 standards. The level of seed infection and the composition of pathogenic fungi were assessed.Results. After eight years of storage at +20°С, pine and spruce seed germination capacity decreased by 13–60%, depending on the year of harvesting. Seed storage at –18°С and –182°С allowed us to prevent seed infection and preserve seed viability. In most cases, the germination energy and germination capacity were negatively correlated with the level of seed infection. The diversity of pathogenic (mold) fungi on the surface of seeds was represented by ten genera; the most common were saprotrophs: Aspergillus P. Micheli, Penicillium Link, Rhizopus Ehrenb., Scopulariopsis Bainier.Conclusion. At the present stage of research, the success of seed storage at low and ultralow temperatures was demonstrated. We recommend cryopreservation for the long-term storage of improved and valuable seeds.

scholarly journals The Effect of Temperature on Seed Germination and Seedling Growth of Two Invasive Plants in Rorippa

2020 ◽  
pp. 39-48
Author(s):  
Qiuli Wang ◽  
Bo Qu ◽  
Juanjuan Mi ◽  
Yufeng Xu ◽  
Meini Shao
Keyword(s):  
Seed Germination ◽  
Invasive Plants ◽  
Seedling Growth ◽  
Petri Dish ◽  
Germination Percentage ◽  
Laboratory Culture ◽  
Liaoning Province ◽  
Effect Of Temperature ◽  
Invasion Mechanism ◽  
Root Shoot Ratio

Aims: By studying the response of seed germination and seedling growth of invasive plants, Rorippa amphibia and Rorippa sylvestris, to temperature, the influence of temperature on the invasive ability of two species of Rorippa were further analyzed, which provided a theoretical basis for revealing the diffusion and invasion mechanism of two invasive plants in Rorippa. Study Design: Seed germination and seedling growth test of two invasive plants in Rorippa at different temperature was studied by means of laboratory culture. The germination percentage, germination index, germination potential of the seeds and the total leaf number, root length, lateral root number, biomass and root shoot ratio of seedlings were determined. Place and Duration of Study: Seeds were collected from the Shenyang Agricultural University of Liaoning Province in July 2018. Experiments were done in the College of Bioscience and Biotechnology, and conducted in April 2019 for a month. Methodology: The petri dish method was used at the experiment of seed germination. The seedling growth experiment was carried out by pot sowing. Results: At the seedling stage.R. amphibia has the strongest tolerance at 30°C, while the R. sylvestris does at 35°C. The low temperature is more beneficial to the accumulations of the seedlings, R. amphibia is the most tolerant at 25°C, and R. sylvestris is at 30°C at the seedling stage. Conclusion: The response of the seeds and seedlings of the two species to temperature was basically the same. Higher temperature promoted seed germination and inhibited seedling growth, while lower temperature inhibited seed germination. The response of seeds and seedlings of R. sylvestris to high temperature makes it more invasive in the process of global warming.

scholarly journals Sensitivity of Seed Germination to Temperature and Light in Helichrysum amorginum

2020 ◽  
Vol 77 (1) ◽  
pp. 7
Author(s):  
Sofia ARGYRIOU ◽  
Panayiota PAPASTYLIANOU ◽  
George STAVROPOULOS ◽  
Angeliki KOUSTA ◽  
Ioanna KAKABOUKI ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seedling Survival ◽  
Germination Percentage ◽  
Effect Of Temperature ◽  
Germination Time ◽  
Randomized Design ◽  
Perennial Shrub ◽  
Mean Germination Time ◽  
Lower Temperature ◽  
Seed Propagation

Helichrysum amorginum is a perennial shrub, endemic on the Greek island of Amorgos and used for medicinal, cosmetic and ornamental purposes. Information concerning germination behavior and seedling survival is considered essential for the establishment and cultivation of wild species. The objective of this study was to investigate the effect of temperature and light on germination of Helichrysum amorginum seeds. A set of experiments was conducted under completely randomized design with four replications of 200 seeds at three different suboptimal germination temperatures (15, 20 and 25°C) in light and dark conditions for 10 days. Seed material was collected from two different fields of the island (Aegiali and Katapola). The germination performance was evaluated by final germination percentage and mean germination time. The effect of temperature on seed germination percentage and mean germination time was significant in all cases. The presence of light enhanced germination, especially at the optimum temperature of 20°C. In most cases the mean germination time increased at the lower temperature of 15°C. Seeds from Aegiali exhibited higher germination ability than the seed lot from Katapola. Knowledge of seed germination behaviour is of major importance in order to establish effective protocols for seed propagation in Helichrysum amorginum.

scholarly journals THE EFFECT OF GLUCOSE AND LIGHT QUALITY ON SEED GERMINATION, VITAMIN E AND CHLOROPHYLL CONTENT OF GREEN BEAN SPROUT (Vigna radiata (L.) Wilcz.)

2015 ◽  
Vol 2 (1) ◽  
pp. 317 ◽  
Author(s):  
Kumala Dewi ◽  
Parmi _
Keyword(s):  
Seed Germination ◽  
Chlorophyll Content ◽  
Vigna Radiata ◽  
Light Quality ◽  
Germination Percentage ◽  
Green Bean ◽  
Dark Condition ◽  
Bean Sprout ◽  
Effect Of Glucose ◽  
Vitamine E

<p>Environmental factors such as the existence of exogenous sugar and light quality during seed germination may affect the growth and quality of seedlings. This experiment was aimed to evaluate the effect of glucose and light quality on seed germination percentage, vitamine E and chlorophyll content in green bean sprout (Vigna radiata (L.) Wilcz). The experiment design used was 5x5 factorial. The main factor was glucose concentration which consisted of 5 levels (0%, 0.5%, 1%, 2.5% or 5 %). The second factor was variation of light quality which consists of 3 different light spectrums namely blue, red or green as well as sunlight and dark condition. Seeds were selected, sterilized and germinated in a petridish containing various level of glucose. Fourty seeds were germinated in each petridish and 5 replicates were used per treatment. Petridish with seeds in it were then placed in a growth chamber having different light quality (blue, red or green), under dark condition or light condition. Growth parameters observed were germination percentage and height of bean sprout. In addition, vitamine E and chlorophyll conten were determined using spectrophotometer. Data were analyzed by using analysis of variance (ANAVA) and followed by Duncan’s Multiple Range Test (DMRT) at significant level of 5%. The results showed that increasing glucose content caused reduction in the germination percentage and height of bean sprouts whereas green and blue light also reduced seed germination. The vitamine E content in green bean sprout grown under red and blue light was relatively similar compared to those grown under sunlight or dark condition, however, green light decreased the vitamine E content in green bean sprout. In addition, the existence of glucose and light quality influenced chlorophyll content in green bean sprout.</p><p><br /><strong>Keywords</strong> : glucose, light quality, green bean, vitamine E, chlorophyll content.</p>

scholarly journals Seed Germination as Assessment for “ex situ” Conservation of Diplotaxis sp

2012 ◽  
Vol 69 (2) ◽  
Author(s):  
Simona Laura LAZAR ◽  
Doru PAMFIL ◽  
Mihai Lucian LUNG
Keyword(s):  
Seed Storage ◽  
Germination Percentage ◽  
Interactive Effects ◽  
Ex Situ ◽  
Physiological Status ◽  
Before And After ◽  
The Status ◽  
Storage Behavior ◽  
Storage Methods ◽  
Selection Of

The effective seed storage methods provide a unique opportunity to preserve large amount of germplasm. Evaluation of the physiological status of the seeds before and after the storage is required for better selection of the methods and lots of seeds for conservation. To evaluate the status of the seeds, 26 accessions of different species were evaluated in two consecutive years. Four replicates of 50 seeds of each population were induced to germinate in 2010 and three replicates of 50 sees in 2011. A 2 × 2x2 factorial experiment investigated the interactive effects of accession, culture medium and year at a temperature >20OC. The germination and the Germination Velocity Index were evaluated. The results showed that there was no loss of viability before and after storage but some of the accessions had germination percentage under 50%. This happened mainly to the seeds gathered from the wild and to one commercial variety in 2010. In 2011 all accessions showed good germination with better results on agar than on filter paper.  Seeds of Diplotaxis sp possess orthodox storage behavior and therefore they are suited for ex situ conservation in seed banks.

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