scholarly journals THE EFFECT OF GLUCOSE AND LIGHT QUALITY ON SEED GERMINATION, VITAMIN E AND CHLOROPHYLL CONTENT OF GREEN BEAN SPROUT (Vigna radiata (L.) Wilcz.)

2015 ◽  
Vol 2 (1) ◽  
pp. 317 ◽  
Author(s):  
Kumala Dewi ◽  
Parmi _
Keyword(s):  
Seed Germination ◽  
Chlorophyll Content ◽  
Vigna Radiata ◽  
Light Quality ◽  
Germination Percentage ◽  
Green Bean ◽  
Dark Condition ◽  
Bean Sprout ◽  
Effect Of Glucose ◽  
Vitamine E

<p>Environmental factors such as the existence of exogenous sugar and light quality during seed germination may affect the growth and quality of seedlings. This experiment was aimed to evaluate the effect of glucose and light quality on seed germination percentage, vitamine E and chlorophyll content in green bean sprout (Vigna radiata (L.) Wilcz). The experiment design used was 5x5 factorial. The main factor was glucose concentration which consisted of 5 levels (0%, 0.5%, 1%, 2.5% or 5 %). The second factor was variation of light quality which consists of 3 different light spectrums namely blue, red or green as well as sunlight and dark condition. Seeds were selected, sterilized and germinated in a petridish containing various level of glucose. Fourty seeds were germinated in each petridish and 5 replicates were used per treatment. Petridish with seeds in it were then placed in a growth chamber having different light quality (blue, red or green), under dark condition or light condition. Growth parameters observed were germination percentage and height of bean sprout. In addition, vitamine E and chlorophyll conten were determined using spectrophotometer. Data were analyzed by using analysis of variance (ANAVA) and followed by Duncan’s Multiple Range Test (DMRT) at significant level of 5%. The results showed that increasing glucose content caused reduction in the germination percentage and height of bean sprouts whereas green and blue light also reduced seed germination. The vitamine E content in green bean sprout grown under red and blue light was relatively similar compared to those grown under sunlight or dark condition, however, green light decreased the vitamine E content in green bean sprout. In addition, the existence of glucose and light quality influenced chlorophyll content in green bean sprout.</p><p><br /><strong>Keywords</strong> : glucose, light quality, green bean, vitamine E, chlorophyll content.</p>

scholarly journals Experimental seed germination for ex situ conservation of Mexican Podostemaceae

2019 ◽  
Vol 97 (3) ◽  
pp. 413 ◽  
Author(s):  
Valeria Flores-Enríquez ◽  
Guillermo Castillo ◽  
Margarita Collazo-Ortega
Keyword(s):  
Seed Germination ◽  
Storage Time ◽  
Light Quality ◽  
Germplasm Conservation ◽  
Seed Storage ◽  
Germination Percentage ◽  
Seed Banks ◽  
Ex Situ ◽  
Effect Of Temperature ◽  
Collection Site

<p><strong>Background</strong>: Podostemaceae are extremely susceptible to local extinction by habitat loss. Since ~70 % of the river systems in Mexico show some degree of water contamination, it is relevant to generate information about seed storage and germination behavior to design germplasm conservation strategies (<em>e.g</em>., <em>ex situ</em> seed banks) of Mexican podostemads.</p><p><strong>Hypotheses</strong>: Seed germination decreases as seed storage time increases. Further, light quality, temperature and collection site influence similarly the germination response of both species.</p><p><strong>Studied species</strong>: <em>Marathrum foeniculaceum</em> Humb. &amp; Bonpl., <em>Noveloa coulteriana</em> (Tul.)<em> </em>C. Philbrick</p><p><strong>Study site and years of study</strong>: 13 different seeds collections (1996-2013), at four locations in the rivers Horcones and Arroyo del Rincon Jalisco, México. </p><p><strong>Methods</strong>: A germination chamber experiment was performed to evaluate the effect of temperature, light quality and storage time on the germination of both species.</p><p><strong>Results</strong>: Seeds lose viability after nine years of storage. In both species, the time to reach the highest Accumulated Germination Percentage (AGP) was faster in seeds of one-two yr compared to seeds of six-seven yr. <em>N. coulteriana</em> have significant differences in Final Germination Percentage (FGP) between collection rivers. Storage time affects AGP of <em>N. </em>coulteriana more than in <em>M. foeniculaceum.</em></p><p><strong>Conclusions</strong>: Both species can form <em>ex situ</em> seed banks up to 8 yr age in paper bags storage. <em>N. coulteriana</em> is more susceptible to variation in storage conditions compared to <em>M. </em><em>foeniculaceum</em>. Collection site affect seed germination after storing.  Long-term conventional <em>ex situ</em> seed germination storage is not a viable strategy to conserve germplasm of Podostemaceae.</p>

scholarly journals Serianthes nelsonii Seed Germination and Seedling Behavior are Minimally Influenced by Chemical and Light Treatment

Horticulturae ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 31 ◽  
Author(s):  
Thomas E. Marler
Keyword(s):  
Seed Germination ◽  
Light Quality ◽  
Red Light ◽  
Germination Percentage ◽  
Growth Responses ◽  
Endangered Tree ◽  
Endangered Tree Species ◽  
Chemical Solutions ◽  
Germination Traits

The role of seed imbibition and light during germination are not known for the critically endangered Serianthes nelsonii Merr. Scarified seeds were pre-soaked in gibberellic acid (GA3) up to 300 mg/L and nitrate solutions of 3000 mg/L to determine if germination was influenced by these treatments. Scarified and imbibed seeds were incubated in high red:far red and low red:far red light to determine the influence of light quality on germination traits. The GA3 and nitrate treatments did not influence germination percentage or timing, but did increase the height of newly emerged seedlings. Moreover, GA3 extended the longevity of cotyledons and shortened the window of time that seedlings required to resume height growth. These growth responses were not sustained, and all seedlings reached heights of 30 cm at a similar number of weeks. The light treatments did not influence any of the germination response traits. The results indicate that imbibing seeds with chemical solutions and providing light in a range of light quality treatments exerted a minimal influence on S. nelsonii seed germination behaviors. Imbibing seeds with water and germinating in darkness is sufficient for achieving the germination of this endangered tree species.

scholarly journals Effect of Aqueous and Ethanol Extract of Acacia nilotica L. Leaves on Seed Germination of Vigna radiata L.

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Nailul Rahmi Aulya
Keyword(s):  
Seed Germination ◽  
Aqueous Extract ◽  
Crude Extract ◽  
Vigna Radiata ◽  
Germination Rate ◽  
Ethanol Extract ◽  
Germination Percentage ◽  
Acacia Nilotica ◽  
Fresh Weight ◽  
Radicle Length

<p>This research aimed to tested the effect of extract on Vigna radiata L. germination. Tested the effect of crude extract of A. nilotica leaves on germination was conducted experimentally using Factorial Completely Randomized Design (FCRD). The first fact or was the type of crude extract and the second factor was levels of extract concentration. The result showed that the application of aqueous and ethanol extract enhanced all parameters of V. radiata L. germination (percentage of seed germination, rate of seed germination, radicle length, seedling fresh weight). Application of lower concentration (0,25%) of aqueous and ethanol extract increased speed of germination, radicle length, and fresh weight of seed. The application of 75% aqueous extract increased the percentage of germination to 100% than control and another treatment. However, further research is needed to confirm the effect of this plant extract on the vegetative growth of Vigna radiata L.</p><p>Keyword: Acacia nilotica, aqueous extract, ethanol extract, seed germinatio</p>

scholarly journals Effects of vermicompost and urea on the seed germination and growth parameters of Vigna mungo L. and Vigna radiata L. Wilzek

2019 ◽  
Vol 11 (2) ◽  
pp. 321-326
Author(s):  
Sanchita Bhattacharya ◽  
Sourav Debnath ◽  
Sanjit Debnath ◽  
Ajay Krishna Saha
Keyword(s):  
Seed Germination ◽  
Leaf Area ◽  
Seedling Growth ◽  
Root Length ◽  
Vigna Radiata ◽  
Growth Parameters ◽  
Germination Percentage ◽  
Shoot Length ◽  
Vigna Mungo ◽  
Inorganic Fertilizer

Growth attributes of Vigna mungo and Vigna radiata crop influenced by level and type of fertilizers. The present study was carried out to evaluate and compare the effects of organic (vermicompost) and inorganic (urea) fertilizers on the germination percentage and seedling growth of V. radiata (Green gram) and V. mungo (Black gram). Fresh weight and dry weight of tested plant samples at 10th days of growth stage were also determined. Vermicompost was used as organic fertilizer and urea as inorganic fertilizer. Experimental results showed that vermicompost and urea both has positive effect on seedling growth parameters of V. mungo and V. radiata [average root length (6.1cm and 6.7cm)] , shoot length (6.5cm and 8.3cm), leaf area (312.2 sq.cm and 334.1 sq.cm] as compared to control set [average root length (4.4cm and 4.3cm)] , shoot length (6cm and 5.9cm), leaf area (282.7 sq.cm and 305.5 sq.cm). But urea exerts negative effect on seed germination percentage in V. mungo and V. radiata (58% and 50%) as compared to control (77%). Vermicompost exhibited better result in above parameters in comparison to urea. V. mungo showed increased value in comparison considering V. radiata in case of organic, inorganic fertilizer treated as well as control sets. As composition of locally available fertilizers is unknown, the application of these fertilizers for improving germination percentage and growth parameters of tested crop need to be evaluated. Present work may provide the suggestive approach for usage of these tested fertilizers in field level trial.

AC Harosprout mungbean

1997 ◽  
Vol 77 (1) ◽  
pp. 111-112 ◽  
Author(s):  
S. J. Park ◽  
T. R. Anderson
Keyword(s):  
Research And Development ◽  
Key Words ◽  
Vigna Radiata ◽  
Green Gram ◽  
Green Bean ◽  
Green Beans ◽  
Bean Sprout ◽  
Development Centre ◽  
Cultivar Description

AC Harosprout is a medium to full-season-maturing and high-yielding mungbean [Vigna radiata (L.) Wilczek] cultivar. It is adapted to southwestern Ontario for the production of green beans for sprouting and other human uses. It was purified from an introduction VC1973A from the Asian Vegetable Research and Development Centre (AVRDC). Key words: Vigna radiata, mungbean, green bean, green gram, bean sprout, cultivar description

IMPACT OF FERTILIZERS APPLICATION ON SEED GERMINATION AND SEEDLING GROWTH OF VIGNA RADIATA

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
SUPRIYA DIXIT ◽  
R. K. GUPTA
Keyword(s):  
Seed Germination ◽  
Leaf Area ◽  
Seedling Growth ◽  
Vigna Radiata ◽  
Agricultural Research ◽  
Dry Weight ◽  
Fertilizer Application ◽  
Research Field ◽  
Chemical Fertilizer ◽  
Seedling Length

Currently, a real challenge for the workers in the agricultural research field is to stop or reduce the use of expensive agrochemicals/ chemical fertilizers which are hazardous to the environment as well as human health. Present study was aimed to improve the growth and obtain optimum yield of Vigna crop with eco-friendly, non-toxic way and to reduce the use of agrochemical/chemical fertilizer application in agricultural activities. A pot experiment was conducted to study the effect of chemical fertilizer (DAP) and biofertilizer ( Rhizobium strain) separately and in combination on seed germination and seedling growth (at 30 days) based on morphological parameters such as seedling length (cm), fresh weight (g), dry weight (g) and leaf area (cm)2 of Vigna radiata (L.) Wilczek. After one month (30 Days) observations, it was found that seedling length, fresh and dry weights and leaf area were maximum in T4 and minimum in T15, T7 and T8 favored improved seedling length and leaf area whereas T7, T8, and T9 favored improved fresh and dry weights as compared to control.

Pengaruh Ekstrak Berbagai Bagian Dari Tanaman Swietenia mahagoni Terhadap Perkecambahan Benih Kacang Hijau Dan Jagung

Agrologia ◽  
2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Stela Wusono ◽  
John M Matinahoru ◽  
CMA Watimena
Keyword(s):  
Seed Germination ◽  
Green Bean ◽  
Bean Seed ◽  
Maize Seed ◽  
Green Beans ◽  
Leaves And Roots ◽  
Swietenia Mahagoni

Swietenia mahagoni is a timber forestry plants have allelopathy toxic, can interfere with the growth of surrounding plants. This study aimed to determine the effects of extracts from various parts of Swietenia mahagoni on seed germination of green beans and corn. This research was conducted at the Laboratory of Silviculture, an extract from a litter, fresh leaves, bark and roots of the Swietenia mahagoni plant and given to the seed germination green beans and corn. The results showed that the extract of fresh leaves and roots Swietenia mahagoni inhibit seed germination green beans and corn, while the provision of litter no effect. Green bean seed has a higher durability of the maize seed to allelopathy of root Swietenia mahagoni.

scholarly journals Effect of H2O-Based Low-Pressure Plasma (LPP) Treatment on the Germination of Bambara Groundnut Seeds

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 338
Author(s):  
Naeem Ahmed ◽  
Asad Masood ◽  
Kim S. Siow ◽  
M. F. Mohd Razip Wee ◽  
Rahmat Zaki Auliya ◽  
...  
Keyword(s):  
Food Security ◽  
Seed Germination ◽  
Chemical Modification ◽  
Low Pressure ◽  
Germination Percentage ◽  
Bambara Groundnut ◽  
Pressure Plasma ◽  
Low Pressure Plasma ◽  
Amine Groups

In general, seed germination is improved by low-pressure plasma (LPP) treatment using precursors such as air, nitrogen, argon, or water (H2O). Here, H2O-based LPP treatment using the optimized parameters of 10 W and 10 s improves the germination of Bambara groundnut seeds by 22%. LPP increases the wettability and roughness of the seed hilum while oxidizing the surface with carboxyl and amine groups. In this H2O-based treatment of Bambara groundnut seeds, combinatory etching and chemical modification facilitated the imbibition process and increased the germination percentage. The success of this method has the potential to be scaled up to solve food security with seeds otherwise facing germination-related issues.

scholarly journals Asymbiotic germination of Vanilla planifolia in relation to the timing of seed collection and seed pretreatments

2021 ◽  
Vol 62 (1) ◽  
Author(s):  
Chih-Hsin Yeh ◽  
Kai-Yi Chen ◽  
Yung-I. Lee
Keyword(s):  
Seed Germination ◽  
Seed Development ◽  
Seed Coat ◽  
Germination Percentage ◽  
Vanilla Planifolia ◽  
Outermost Layer ◽  
Seed Collection ◽  
Seed Pretreatment ◽  
Immature Seeds ◽  
Mature Seeds

Abstract Background Vanilla planifolia is an important tropical orchid for production of natural vanilla flavor. Traditionally, V. planifolia is propagated by stem cuttings, which produces identical genotype that are sensitive to virulent pathogens. However, propagation with seed germination of V. planifolia is intricate and unstable because the seed coat is extremely hard with strong hydrophobic nature. A better understanding of seed development, especially the formation of impermeable seed coat would provide insights into seed propagation and conservation of genetic resources of Vanilla. Results We found that soaking mature seeds in 4% sodium hypochlorite solution from 75 to 90 min significantly increased germination. For the culture of immature seeds, the seed collection at 45 days after pollination (DAP) had the highest germination percentage. We then investigated the anatomical features during seed development that associated with the effect of seed pretreatment on raising seed germination percentage. The 45-DAP immature seeds have developed globular embryos and the thickened non-lignified cell wall at the outermost layer of the outer seed coat. Seeds at 60 DAP and subsequent stages germinated poorly. As the seed approached maturity, the cell wall of the outermost layer of the outer seed coat became lignified and finally compressed into a thick envelope at maturity. On toluidine blue O staining, the wall of outer seed coat stained greenish blue, indicating the presence of phenolic compounds. As well, on Nile red staining, a cuticular substance was detected in the surface wall of the embryo proper and the innermost wall of the inner seed coat. Conclusion We report a reliable protocol for seed pretreatment of mature seeds and for immature seeds culture based on a defined time schedule of V. plantifolia seed development. The window for successful germination of culturing immature seed was short. The quick accumulation of lignin, phenolics and/or phytomelanins in the seed coat may seriously inhibit seed germination after 45 DAP. As seeds matured, the thickened and lignified seed coat formed an impermeable envelope surrounding the embryo, which may play an important role in inducing dormancy. Further studies covering different maturity of green capsules are required to understand the optimal seed maturity and germination of seeds.

Sign in / Sign up

Export Citation Format

Share Document