Assessment of the mannose inhibition on tobacco regeneration for establishment of a mannose selection system for transgenic plants

Recently, the application of environmentally friendly methods in transgenic-plant selection has been exploited with an interest to contribute to the commercialization of transgenic products. In this study, mannose was chosen in selecting transgenic tobacco. The ability of mannose to inhibit tobacco regeneration was assessed through three stages: creating multi-shoot, elongating shoot, and rooting of tobacco cultivars K326 and C9-1. The results showed that mannose concentrations at 30 g/l and 20 g/l are suitable for creating multi-shoot of K326 and C9-1, respectively. In addition, mannose concentration at 30 g/l in combination with 7,5 g/l sucrose is fit for elongating shoot while its concentration at 30 g/l is appropriate for rooting transgenic tobacco in both K326 and C9-1 cultivars. The mannose selection of transgenic tobacco using gus gene showed that the transgenic effectiveness is ranging 16-17% of K326 and 9-11% of C9-1 cultivars. The successful establishment of mannose selection system in transgene tobacco contributes to generate bio-safety transgenic plants.

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Approaches to insect resistance using transgenic plants

Philosophical Transactions of the Royal Society B Biological Sciences ◽

10.1098/rstb.1993.0158 ◽

1993 ◽

Vol 342 (1301) ◽

pp. 279-286 ◽

Cited By ~ 45

Keyword(s):

Transgenic Plants ◽

Transgenic Plant ◽

Transgenic Tobacco ◽

Protease Inhibitors ◽

Resistance Genes ◽

Tobacco Plant ◽

Brown Planthopper ◽

Plant Defence

Crops resistant to insect attack offer a different strategy of pest control to indiscriminate pesticide usage, which has undesirable effects on both the environment and humans. Transgenic plant technology can be a useful tool in producing resistant crops, by introducing entirely novel resistance genes into a plant species. Although most work in this area has focused on the use of genes encoding insecticidal Bacillus thuringiensis δ-endotoxins in transgenic plants, an alternative approach is to use plant genes which encode proteins with insecticidal properties. Protease inhibitors are involved in endogenous plant defence against insects. Over-expression of several inhibitors from constitutive promoters has been shown to afford protection in transgenic tobacco plants against attack by lepidopteran larvae. However, the degree of protection is not sufficiently high, and shows species- and inhibitor-specific effects. By assaying the interactions of protease inhibitors with insect gut proteases in vitro , the most effective inhibitor can be selected for a particular insect species. Data from bioassays of insects using artificial diets, and with transgenic plants, suggest that the in vitro assay of relative inhibitor effectiveness is consistent with the effects of different inhibitors on insect development and survival in vivo . Development of this techniology is considered. A different approach must be taken with sucking insect pests, as they do not rely on proteolysis for nutrition, and as Bt toxins effective against hom opterans have not been reported to date. Bioassay in artificial diet was used to identify plant proteins with insecticidal effects on the rice brown planthopper (a model homopteran). The lectin from snowdrop (GNA) was found to be the most effective of the proteins tested. GNA was shown to be present in the phloem sap of a transgenic tobacco plant transformed with a chimeric gene construct, containing the rice sucrose synthase-1 gene promoter and the GNA coding sequence, by immunoassay of honeydew produced by aphids feeding on it. GNA is also insecticidal to the aphid Myzus persicae , which will feed on tobacco, and thus a bioassay of transgenic tobacco, to ‘prove’ the technology, can be carried out. The effects of combining different resistance genes in the same transgenic plant to improve the effectiveness of protection are discussed, and exemplified.

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Comparison of a kanamycin versus hygromycin resistance gene in transgenic plant selection of Arabidopsis thaliana L.

10.35841/cell-science.1000101 ◽

2017 ◽

Vol 01 (01) ◽

Cited By ~ 1

Author(s):

Abdur Rashid

Keyword(s):

Arabidopsis Thaliana ◽

Transgenic Plant ◽

Resistance Gene ◽

Hygromycin Resistance ◽

Plant Selection ◽

Hygromycin Resistance Gene ◽

Selection Of

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Targeted Protection of Mitochondria of Mesophyll Cells in Transgenic CYP11A1 CDNA Expressing Tobacco Plant Leaves After NaCl-Induced Stress Damage

Proceedings of the Latvian Academy of Sciences Section B Natural Exact and Applied Sciences ◽

10.2478/prolas-2018-0048 ◽

2018 ◽

Vol 72 (6) ◽

pp. 334-340 ◽

Cited By ~ 2

Author(s):

Ekaterina N. Baranova ◽

Marat R. Khaliluev ◽

Svetlana G. Spivak ◽

Lilia R. Bogoutdinova ◽

Valery N. Klykov ◽

...

Keyword(s):

Transgenic Plants ◽

Transgenic Plant ◽

Transgenic Tobacco ◽

Tobacco Plant ◽

Plant Mitochondria ◽

Peptide Sequence ◽

Dense Matrix ◽

Salinity Treatment ◽

Mesophyll Cells ◽

Signal Peptide Sequence

Abstract Recently we have showed that the expression of the mammalian CYP11A1 cDNA in plants confers their resistance to abiotic and biotic stresses. To determine the role of heterologous expression of cytochrome P450scc cDNA in resistance to ROS (radical oxygen species) dependent abiotic stresses, the structural changes of mitochondria and peroxisomes were studied under 150 mM NaCl-induced 14-day salinity treatment on juvenile tobacco plants in in vitro culture. Ultrastructural analysis of mesophyll cells of transgenic tobacco leaves constitutively expressing CYP11A1 cDNA was performed. Under NaCl stress, a change in shape from rounded to elon-gated, reduced section area, formation of branched mitochondria, as well as the emergence of triangular and rhomboid cristae, densification of a mitochondrial matrix, increase in density of contrasting membranes and their thickness were observed in non-transgenic plants. Transgenic plants without stress applied had mitochondria with rounded and elongated shape, twice as small as in non-transgenic plants, with a dense matrix and sinuous cristae. Surprisingly, the effect of NaCl led to increase in size of mitochondria by 1.5 times, decomposition of matrix and the emergence in organelles of light zones presumably containing mitochondrial DNA strands. Thus, the structural organisation of transgenic plant mitochondria under salinity treatment was comparable to that of non-transgenic plants under native conditions. It was also noted that the transgenic plant peroxisomes differed in non-transgenic tobacco both in normal condition and under the action of NaCl. The observed differences in ultrastructural organisation of mitochondria not only support our earlier notion about successful incorporation of the mature P450scc into this organelle, but for the first time demonstrate that the mammalian CYP11A1 signal peptide sequence could be efficiently used in the formation of targeted mitochondria protection of plants from salinity-induced damage.

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Comparison of a kanamycin versus hygromycin resistance gene in transgenic plant selection of Arabidopsis thaliana L.

10.35841/cell-science.1.1.1-2 ◽

2018 ◽

Vol 01 (01) ◽

Author(s):

Abdur Rashid

Keyword(s):

Arabidopsis Thaliana ◽

Transgenic Plant ◽

Resistance Gene ◽

Hygromycin Resistance ◽

Plant Selection ◽

Hygromycin Resistance Gene ◽

Selection Of

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Overexpression of the pitaya phosphoethanolamine N-methyltransferase gene (HpPEAMT) enhanced simulated drought stress in tobacco

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-021-02040-3 ◽

2021 ◽

Author(s):

Ai-Hua Wang ◽

Lan Yang ◽

Xin-Zhuan Yao ◽

Xiao-Peng Wen

Keyword(s):

Drought Stress ◽

Stress Response ◽

Transgenic Plants ◽

Drought Tolerance ◽

Transgenic Tobacco ◽

Sequence Similarity ◽

Amino Acid Sequence Similarity ◽

Transgenic Tobacco Plants ◽

Wild Type ◽

Tobacco Plants

AbstractPhosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.

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Mice Orally Immunized with a Transgenic Plant Expressing the Glycoprotein of Crimean-Congo Hemorrhagic Fever Virus

Clinical and Vaccine Immunology ◽

10.1128/cvi.05352-11 ◽

2011 ◽

Vol 18 (12) ◽

pp. 2031-2037 ◽

Cited By ~ 32

Author(s):

S. M. Ghiasi ◽

A. H. Salmanian ◽

S. Chinikar ◽

S. Zakeri

Keyword(s):

Transgenic Plants ◽

Transgenic Plant ◽

Disease Transmission ◽

Clinical Symptoms ◽

Hemorrhagic Fever ◽

Oral Immunization ◽

Negative Control ◽

Significant Rise ◽

Knockout Mouse Model ◽

Crimean Congo Hemorrhagic Fever

ABSTRACTWhile Crimean-Congo hemorrhagic fever (CCHF) has a high mortality rate in humans, the associated virus (CCHFV) does not induce clinical symptoms in animals, but animals play an important role in disease transmission to humans. Our aim in this study was to examine the immunogenicity of the CCHFV glycoprotein when expressed in the root and leaf of transgenic plants via hairy roots and stable transformation of tobacco plants, respectively. After confirmatory analyses of transgenic plant lines and quantification of the expressed glycoprotein, mice were either fed with the transgenic leaves or roots, fed the transgenic plant material and injected subcutaneously with the plant-made CCHFV glycoprotein (fed/boosted), vaccinated with an attenuated CCHF vaccine (positive control), or received no treatment (negative control). All immunized groups had a consistent rise in anti-glycoprotein IgG and IgA antibodies in their serum and feces, respectively. The mice in the fed/boosted group showed a significant rise in specific IgG antibodies after a single boost. Our results imply that oral immunization of animals with edible materials from transgenic plants is feasible, and further assessments are under way. In addition, while the study of CCHF is challenging, our protocol should be further used to study CCHFV infection in the knockout mouse model and virus neutralization assays in biosafety level 4 laboratories.

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Genetic transformation of Torenia fournieri using the PMI/mannose selection system

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-007-9260-1 ◽

2007 ◽

Vol 90 (1) ◽

pp. 103-109 ◽

Cited By ~ 9

Author(s):

Hong-Qing Li ◽

Pei-Jing Kang ◽

Mei-Lan Li ◽

Mei-Ru Li

Keyword(s):

Genetic Transformation ◽

Selection System ◽

Torenia Fournieri ◽

Mannose Selection

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Integration of the Motor Carrier Safety Status Measurement System into the Roadside Inspection Selection System

Transportation Research Record Journal of the Transportation Research Board ◽

10.3141/1734-09 ◽

2000 ◽

Vol 1734 (1) ◽

pp. 59-63

Author(s):

Brenda M. Lantz

Keyword(s):

Measurement System ◽

Safety Data ◽

Safety Performance ◽

Similar Data ◽

Selection System ◽

Department Of Transportation ◽

Commercial Vehicles ◽

Information Systems Management ◽

Motor Carrier ◽

Selection Of

The roadside Inspection Selection System (ISS) was developed in response to a 1995 congressional mandate that called for the use of prior carrier safety data to guide the selection of commercial vehicles and drivers for roadside inspections. The program was developed in part by the Federal Motor Carrier Safety Administration (FMCSA) of the U.S. Department of Transportation. As ISS has developed, FMCSA’s Performance and Registration Information Systems Management (PRISM) program has also been evolving. One objective of PRISM is to identify relatively unsafe carriers by assigning Safety Status Measurement System (SafeStat) scores and also encouraging those drivers to improve their safety performance or risk losing registration privileges. SafeStat was designed to prioritize carriers for monitoring and compliance reviews, but ISS was designed to prioritize carriers for roadside inspection. Both algorithms, however, use similar data to define a relatively unsafe carrier. It would be advantageous therefore to have a single uniform rating system for all FMCSA programs. This research briefly describes the PRISM and SafeStar algorithms; discusses the integration of the SafeStat algorithm into ISS; and presents conclusions on the initial testing of the resulting system, ISS-2. An analysis of over 213,000 roadside inspections reveals that ISS-2 is as effective as the original ISS in meeting the goals for which it was designed. It successfully identifies and prioritizes for roadside inspection the vehicles and drivers of carriers with poor prior safety performance, as well as those with few or no previous inspections. In addition, safety inspectors who have tested the system say they are pleased with the new algorithm and its added features.

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