scholarly journals Ensifer sp. CNN3 - AN INDIGENOUS BACTERIAL STRAIN WITH PLANT GROWTH PROMOTING AND ORGANOPHOSPHORUS DEGRADING PROPERTIES IN VIETNAM

2021 ◽  
Vol 59 (1) ◽  
pp. 9
Author(s):  
Chi Kim Hoang ◽  
Trần Thị Như Hằng
Keyword(s):  
Plant Growth ◽  
Bacterial Strain ◽  
Bacterial Isolate ◽  
Soil Samples ◽  
Chemical Fertilizer ◽  
Northern Vietnam ◽  
Plant Growth Promoting ◽  
Test Soil ◽  
Growth Promoting

An indigenous effective bacterial strain has been isolated from soil samples of contaminated tea farming site in Northern Vietnam with chlorpyrifos degradability and plant growth promoting characteristics. The bacterial isolate was determined to belong to genus Ensifer (syn. Sinorhizobium), and its degrading property for OPP substrate chlorpyrifos in culture as well as in test soil were studied. The results open a prospect of applying the dual-effective bacterial strain in agriculture practices either to reduce the use of chemical fertilizer or to remediate OPP contaminated planting soils.

scholarly journals Isolation and Evaluation of Bacteria Exhibiting Multiple Plant Growth Traits in the Rhizosphere of Yellow Bell Pepper (Capsicum chinense)

2019 ◽  
pp. 1-6
Author(s):  
E. C. Chinakwe ◽  
N. U. Nwogwugwu ◽  
V. I. Ibekwe ◽  
P. O. Chinakwe ◽  
E. O. Egbadon ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Plant Growth ◽  
Rhizosphere Soil ◽  
Growth Traits ◽  
Soil Samples ◽  
Bell Pepper ◽  
Capsicum Chinense ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Plant Growth Promoting Traits

Aim: The study identified and evaluated bacteria exhibiting multiple plant growth traits in the Rhizosphere of Yellow Bell Pepper (Capsicum chinense).                                                              Study Design: Seeds of Capsicum chinense were planted in a soil and allowed to grow. After five eeks of planting, soil samples from the rhizosphere were collected and the bacterial community present in the rhizosphere soil of Capsicum chinense was studied. The isolated organisms were assessed for their ability to produce plant growth promoting traits. Place and Duration of Study: This study was carried out at an agricultural research farmland in the Federal University of Technology, Owerri, Nigeria. Methodology: Seeds of Capiscum chinense were planted in the soil samples in a greenhouse. Rhizosphere soil was collected for analysis to identify the bacterial composition of the rhizosphere soil.                                                                                                                                               Results: In this study the presence of Bacillus cereus, Staphylococcus aureus, Corynebacterium sp, Enterococcus feacalis and Bacillus polymyxa were evident in the rhizosphere samples collected. All isolates showed multiple plant growth promoting traits except Staphylococcus aureushich was positive for hydrogen cyanide production only. Conclusion: The results from this study showed that the bacterial community present in the soil can be used to effect significant vegetative crop yield and agricultural production. The isolated rhizobacteria can be formulated as bio-fertilizers or bioinnoculants, etc.

scholarly journals Bambara Groundnut Rhizobacteria Antimicrobial and Biofertilization Potential

2020 ◽  
Author(s):  
Caroline F. Ajilogba ◽  
Olubukola O. Babalola ◽  
Patrick Adebola ◽  
Rasheed Adeleke
Keyword(s):  
Acetic Acid ◽  
Food Security ◽  
Plant Growth ◽  
Hydrogen Cyanide ◽  
Indole Acetic Acid ◽  
Soil Samples ◽  
Bambara Groundnut ◽  
Dual Culture ◽  
Plant Growth Promoting ◽  
Growth Promoting

AbstractBambara groundnut, an underutilized crop has been proved to be an indigenous crop in Africa with the potential for food security. The rhizosphere of Bambara groundnut like other legumes contains several important bacteria that have not been explored for their plant growth-promoting properties. The aim of this research was to determine the potentials of rhizobacteria from Bambara groundnut soil samples as either biofertilizer or biocontrol agents or both to help provide sustainable agriculture in Africa and globally. Analyses of Bambara groundnut rhizospheric soil samples included chemical analysis such as nitrogen content analysis using extractable inorganic nitrogen method as well as cation exchangeable capacity using ammonium acetate method. Plant growth-promoting properties of isolated rhizobacteria tested include indole acetic acid, hydrogen cyanide, phosphate solubilization, 1-aminocyclopropane-1-carboxylate and ammonia production activities using standard methods. In addition, antifungal assay dual culture method was used to analyze the biocontrol properties of the isolates. Phylogenetic analysis using 16S rRNA was also carried out on the isolates. Isolated rhizobacteria from bambara groundnut rhizosphere were cultured. All the isolates were able to produce ammonia and 1-aminocyclopropane-1-carboxylate while 4.65%, 12.28% and 27.91% produced Hydrogen cyanide, Indole acetic acid and solubilized phosphate respectively, making them important targets as biocontrol and biofertilizer agents. The growth of Fusarium graminearum was suppressed in vitro by 6.98% of the isolates. Plant growth promoting activities of rhizobacteria from bambara groundnut rhizosphere reveals that it has great potentials in food security as biofertilizer and biocontrol agent against fungal and bacterial pathogens.

scholarly journals Novel Bacillus cereus Strain, ALT1, Enhance Growth and Strengthens the Antioxidant System of Soybean under Cadmium Stress

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 404
Author(s):  
Atlaw Anbelu Sahile ◽  
Muhammad Aaqil Khan ◽  
Muhammad Hamayun ◽  
Muhammad Imran ◽  
Sang-Mo Kang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Culture Medium ◽  
Bacterial Isolate ◽  
Cadmium Stress ◽  
Cd Stress ◽  
Pgp Traits ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Soybean Plants

Cadmium stress significantly decreases agricultural productivity worldwide. Plant growth-promoting rhizobacteria (PGPR) are eco-friendly and inexpensive tool for mitigating heavy metal stress in crops. We isolated rhizospheric bacteria and screened them for various plant growth-promoting (PGP) traits as well as Cd tolerance. Only 6 bacterial isolates out of 55 assessed showed multiple PGP traits in response to different Cd concentrations. The Bacillus cereus ALT1 strain showed high tolerance to increased Cd amounts in the culture medium, while secreting indole-3-acetic acid (IAA) and organic acids into the culture medium. High Cd concentrations (0.7 mM, 1.4 mM, and 2.1 mM) reduced soybean shoot and root length, root/shoot fresh and dry weight, as well as chlorophyll content; however, inoculation with the bacterial isolate ALT1 mitigated Cd stress and enhanced both soybean growth parameters and chlorophyll content. It also decreased abscisic acid (ABA) amounts, enhanced salicylic acid (SA) production, and promoted antioxidant response by increasing total proteins (TP) and superoxide dismutase (SOD), while decreasing glutathione (GSH) content, lipid peroxidation (LPO), peroxidase (POD), superoxide anion (SOA), and polyphenol oxidase (PPO) in soybean plants. In addition, inductively coupled plasma mass spectrometry (ICP-MS) showed that soybean plants treated with the bacterial isolate ALT1 enhanced K uptake and decreased Cd amounts in comparison to control plants. The present study reveals that Cd-tolerant bacterial isolate ALT1 can alleviate Cd toxicity on plants by increasing their growth, thus imposing itself as an eco-friendly bio-fertilizer under Cd stress.

scholarly journals Interaction of Kreyellidae sp. and plant growth promoting bacteria influences the soil characteristics and root structure of rice plants

2021 ◽  
Author(s):  
Komal A. Chandarana ◽  
Rinka S. Pramanik ◽  
Natarajan Amaresan
Keyword(s):  
Plant Growth ◽  
Combined Treatment ◽  
Soil Microbial Communities ◽  
Soil Samples ◽  
Plant Growth Promoting Bacteria ◽  
Root Structure ◽  
Combined Treatments ◽  
Rice Plants ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Plant growth and productivity depend on the complex and dynamic interaction between the plant roots and soil microflora. At present, the research on rhizosphere associated microbes’ is largely focused on bacteria and fungi; whereas the interaction of soil protists with plants and other microbes remain unexplored. The present study aimed to investigate the impact of a ciliate (Kreyellidae sp. C5) and two plant growth-promoting bacteria (PGPB) i.e., Pseudomonas sp. (Ps) and Enterobacter sp. (Ec), on the growth of rice plants. It was observed that the protist-PGPB interaction significantly modified the root structure leading to an enhanced outgrowth of lateral roots (272.08% − 380.41%) and seminal roots (190.40% -250.45%), in addition to an increase in the primary root length (Turkey’s HSD, p < 0.05). The Phospholipid Fatty Acid (PLFA) analysis indicated a striking shift in the overall soil microbial communities due to the presence of a predator. The combined treatments (with C5Ps and C5Ec) further increased the Microbial Carbon Biomass (MBC) to 223.59% and 310.57% as compared to control and PGPB treatments respectively. A similar enhancement of dehydrogenase enzyme activity was observed in soil samples of rice plants on combined treatments. In contrast, the alkaline phosphatase and fluorescein diacetate enzyme activities were recorded to be more in soil samples treated with PGPB. The combined treatment of rice plants also enhanced the uptake of N and P moderately, as compared to PGPB treated plants. However, this enhancement was significant compared to control plants. The colony-forming unit (CFU) and most probable number (MPN) was found to be more in C5Ec (131.0 ± 3.70×1011 and 5.12 ± 0.06) and C5Ps (24.10 ± 2.19×1010 and 10.52 ± 0.39), as compared to control and PGPB treated soil samples. In conclusion, this is the first study that demonstrates significant modification of root structure and increased nutrient uptake by rice plants through interaction between Kreyellidae sp. and PGPB. In addition, we also report improved respiration and diverse microbial population in soil samples on combined treatment of rice plants.

scholarly journals Inorganic Chemical Fertilizer Application to Wheat Reduces the Abundance of Putative Plant Growth-Promoting Rhizobacteria

2021 ◽  
Vol 12 ◽  
Author(s):  
Tessa E. Reid ◽  
Vanessa N. Kavamura ◽  
Maïder Abadie ◽  
Adriana Torres-Ballesteros ◽  
Mark Pawlett ◽  
...  
Keyword(s):  
16S Rrna ◽  
Plant Growth ◽  
16S Rrna Gene ◽  
Fertilizer Application ◽  
Chemical Fertilizer ◽  
Rrna Gene ◽  
Plant Growth Promoting ◽  
Total Community ◽  
Inorganic Chemical ◽  
Growth Promoting

The profound negative effect of inorganic chemical fertilizer application on rhizobacterial diversity has been well documented using 16S rRNA gene amplicon sequencing and predictive metagenomics. We aimed to measure the function and relative abundance of readily culturable putative plant growth-promoting rhizobacterial (PGPR) isolates from wheat root soil samples under contrasting inorganic fertilization regimes. We hypothesized that putative PGPR abundance will be reduced in fertilized relative to unfertilized samples. Triticum aestivum cv. Cadenza seeds were sown in a nutrient depleted agricultural soil in pots treated with and without Osmocote® fertilizer containing nitrogen-phosphorous-potassium (NPK). Rhizosphere and rhizoplane samples were collected at flowering stage (10 weeks) and analyzed by culture-independent (CI) amplicon sequence variant (ASV) analysis of rhizobacterial DNA as well as culture-dependent (CD) techniques. Rhizosphere and rhizoplane derived microbiota culture collections were tested for plant growth-promoting traits using functional bioassays. In general, fertilizer addition decreased the proportion of nutrient-solubilizing bacteria (nitrate, phosphate, potassium, iron, and zinc) isolated from rhizocompartments in wheat whereas salt tolerant bacteria were not affected. A “PGPR” database was created from isolate 16S rRNA gene sequences against which total amplified 16S rRNA soil DNA was searched, identifying 1.52% of total community ASVs as culturable PGPR isolates. Bioassays identified a higher proportion of PGPR in non-fertilized samples [rhizosphere (49%) and rhizoplane (91%)] compared to fertilized samples [rhizosphere (21%) and rhizoplane (19%)] which constituted approximately 1.95 and 1.25% in non-fertilized and fertilized total community DNA, respectively. The analyses of 16S rRNA genes and deduced functional profiles provide an in-depth understanding of the responses of bacterial communities to fertilizer; our study suggests that rhizobacteria that potentially benefit plants by mobilizing insoluble nutrients in soil are reduced by chemical fertilizer addition. This knowledge will benefit the development of more targeted biofertilization strategies.

scholarly journals Isolasi dan Karakterisasi Bakteri Pemacu Pertumbuhan Tanaman (Plant Growth Promoting Rhizobacteria) Asal Tanah Desa Akar-Akar, Lombok Utara

2021 ◽  
Vol 6 (2) ◽  
pp. 70
Author(s):  
Shania Corolla Azzahra ◽  
Yunus Effendy ◽  
Sudono Slamet
Keyword(s):  
Plant Growth ◽  
Plant Root ◽  
Plant Growth Promoting Rhizobacteria ◽  
Soil Samples ◽  
Clear Zone ◽  
Bacterial Colony ◽  
Plant Growth Promoting ◽  
The Media ◽  
Growth Promoting ◽  
Plant Root System

<p><strong>Plant Root Growth Promoting Bacteria or PGPR are bacteria that colonize plant rooting areas or the rhizosphere to increase the quality or quantity of plant growth. PGPR can provide dissolved phosphate for plants to be absorbed by the plant root system since 95-99% of phosphate in nature is present in insoluble form. Soil samples from different irrigation systems from Akar-akar village were isolated through serial dilution techniques and grown on NA media shown that the higher the dilution, the fewer colonies that grew on the media. Macroscopic and microscopic observations were made to see the criteria for bacteria, shown that the bacteria in the three soil samples were gram negative and was known that bacillus and cocobacillus bacteria present in the three soil samples. Coccus bacteria was found in soil without irrigation and drip-surface irrigated soil, while streptococcus bacteria was found in drip-surface and drip-subsurface irrigated soil. As many as 22 bacterial isolates were isolated and grown on Pikovskaya media, only one bacterial colony was phosphate solvent through a clear zone that grew around the bacterial colony. The bacterial colony has a phosphate dissolving index (IPF) of 250, the ratio between colony diameter and clear zone diameter was 1: 1.5.</strong></p><p><strong>Keywords – </strong><em>PGPR, Drip-surface irrigated soil, Drip-subsurface irrigated soil</em></p>

scholarly journals Effects of Selected Functional Bacteria on Maize Growth and Nutrient Use Efficiency

2020 ◽  
Vol 8 (6) ◽  
pp. 854 ◽  
Author(s):  
Amelia Tang ◽  
Ahmed Osumanu Haruna ◽  
Nik Muhamad Ab. Majid ◽  
Mohamadu Boyie Jalloh
Keyword(s):  
Plant Growth ◽  
Organic Fertilizer ◽  
Nutrient Use Efficiency ◽  
Nutrient Content ◽  
Chemical Fertilizer ◽  
Nutrient Use ◽  
Chemical Fertilization ◽  
Plant Growth Promoting ◽  
Use Efficiency ◽  
Growth Promoting

Plant growth-promoting rhizobacteria (PGPR), which include isolates from genera Paraburkholderia, Burkholderia and Serratia, have received attention due to their numerous plant growth-promoting mechanisms such as their ability to solubilize insoluble phosphates and nitrogen-fixation. However, there is a dearth of information on the potential plant growth-promoting effects of these three groups of bacteria on non-legumes such as maize. This study determined the influences of the aforementioned strains on soil properties, maize growth, nutrient uptake and nutrient use efficiency. A pot trial using maize as a test crop was done using a randomized complete block design with 7 treatments each replicated 7 times. The treatments used in this study were: Control (no fertilizer), chemical fertilizer (CF), organic-chemical fertilizers combination without inoculum (OCF) and with inocula consisting of single strains [cellulolytic bacteria (TC), organic fertilizer and chemical fertilizer with N-fixing bacteria (TN), organic fertilizer and chemical fertilizer with P-solubilizing bacteria (TP)) and three-strain inocula (TCNP), respectively. The variables measured included plant growth and nutrient content, soil nutrient content and functional rhizospheric bacterial populations. Paraburkholderia nodosa NB1 and Burkholderia cepacia PB3 showed comparable effects on maize biomass and also improved N and P use efficiencies when compared to full chemical fertilization. Nitrogen-fixing rhizobacteria had a positive effect on above-ground biomass of maize. Paraburkholderia nodosa NB1 improved soil total C and organic matter contents, besides being the only bacterial treatment that improved K use efficiency compared to OCF. The results suggest that P. nodosa NB1 and B. cepacia PB3 have potential usage in bio-fertilizers. In contrast, treatments with Serratia nematodiphila C46d and consortium strains showed poorer maize nutrient uptake and use efficiency than the other single strain treatments. Bacterial treatments generally showed comparable or higher overall N and P use efficiencies than full chemical fertilization. These findings suggest that at least half the amounts of N and P fertilizers could be reduced through the use of combined fertilization together with beneficial bacteria.

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