scholarly journals QUALITY AND MICROBIOLOGICAL SAFETY OF A DAILY MORTALITY COMPOST PRODUCED IN A VETERINARY FACULTY AT SÃO PAULO STATE

2021 ◽  
Vol 9 (1) ◽  
pp. 18-28
Author(s):  
Sonia Villamizar Cancelado
Keyword(s):  
Culture Media ◽  
Phytopathogenic Fungi ◽  
Sao Paulo ◽  
Molecular Techniques ◽  
Experimental Unit ◽  
Human Consumption ◽  
São Paulo ◽  
Daily Mortality ◽  
E Coli ◽  
Veterinary Faculty

Introduction: Daily and outbursts mortality composting have been identified as one of the finest methods for final disposal of animal corpses, but the probable threat of pathogens transmission truly limits its use. Materials and Methods:  In this study we evaluated the quality and microbiological biosafety of a compost produced in daily mortality experimental unit composting at the Universidade Estadual Paulista in the state of Sao Paulo, Brazil. Settled compost sample was evaluated in order to determine the presence and counting of coliforms and Salmonella sp. and the pathotypes of E. coli STEC, EPEC and EHEC using culture and molecular techniques.  The occurrence of frequent soil borne phytopathogenic fungi was also estimated using selective and differential microbiological culture media. Results and Discussion: The occurrence of pathogenic E. coli, Salmonella sp and phytopathogenic fungi were negative. Coliforms level was 3.05 log10/g. Concussions: The results showed that daily mortality composting method is effective to reduce pathogenic microorganisms, however, in order to add the product on crops or plants such as vegetables that are for direct human consumption, additional tests must be performed to assess the presence of viral pathogens and endospores forming bacteria.

scholarly journals Microbiological study of vinaigrette salad sold at pasty stalls in street markets in the City of São Paulo, Brazil

2021 ◽  
Vol 10 (11) ◽  
pp. e504101119738
Author(s):  
Sheila Chagas Mendes ◽  
Juliane Vismari de Oliveira ◽  
Katharyna Cardoso de Gois ◽  
Jorge Luiz Pinto ◽  
Fernando Luiz Affonso Fonseca ◽  
...  
Keyword(s):  
Culture Media ◽  
Fungal Growth ◽  
Hepatic Abscess ◽  
Sao Paulo ◽  
Fecal Coliforms ◽  
São Paulo ◽  
E Coli ◽  
High Prevalence ◽  
Biochemical Analyses ◽  
The City

Foodborne diseases (FBD) present high prevalence worldwide and more than 250 types have been reported. The main agents are Staphylococcus aureus, Escherichia coli, Salmonella and certain parasites. The habit of consuming foods sold in street markets in the city of São Paulo exposes consumers to the risk of acquiring FBD. 25 samples of vinaigrette salad were collected in the central, northern, southern, eastern and western regions of the city of São Paulo, Brazil. The samples were inoculated in selective, enriched culture media and biochemical analyses were performed. Bacterial and fungal growth occurred in all samples. The microorganisms detected were E. coli (64%), Enterobacter (60%), S. aureus (52%), Klebsiella (40%), Proteus sp. (32%), Shigella sp. (28%), Citrobacter sp. (16%), Edwardsiella sp. (12%), Alcaligenes sp. (8%), Serratia sp. (8%), Salmonella sp. (4%), Pseudomonas sp. (4%) and; 72% were positive for aflatoxins. Twelve species were identified, 50% of which can cause FBD. S. aureus and fecal coliforms can cause FBD, while Alcaligenes sp. can cause nosocomial infections and Edwardsiella sp. can cause hepatic abscess, meningitis and septicemia. Aflatoxins may cause mycotoxicoses and liver cancer. Therefore, inspections, and prevention and awareness measures should be reinforced to minimize the risks of contracting FBD from foods sold in street markets in the city of São Paulo.

scholarly journals Ocorrência dos principais agentes bacterianos e parasitários em fezes diarreicas de bezerros búfalos nos estados de São Paulo e Paraná

2014 ◽  
Vol 81 (2) ◽  
pp. 180-185 ◽  
Author(s):  
Lilian Gregory ◽  
Rodolfo Santos Rossi ◽  
João Padilha Gandara Mendes ◽  
Natalie Neuwirt ◽  
Eduardo Carvalho Marques ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Sao Paulo ◽  
São Paulo ◽  
Salmonella Spp ◽  
Toxocara Vitulorum ◽  
E Coli ◽  
Eimeria Spp

A população de bubalinos estimada no Brasil é de aproximadamente 3 milhões de animais, encontrando-se distribuídos em todos os Estados brasileiros, com crescimento médio anual de 12%. Apesar disso, os trabalhos realizados buscando os avanços na bubalinocultura são escassos. Em função da complexidade etiológica da diarreia em bubalinos e da falta de informações recentes nesta área, o presente estudo teve como objetivo avaliar a ocorrência dos principais agentes bacterianos e parasitários envolvidos na diarreia de bezerros búfalos lactentes, de explorações leiteiras semi-intensivas e intensivas em regiões dos estados de São Paulo e Paraná. De março de 2010 a junho de 2011, foram colhidas 53 amostras para exame coproparasitológico e 46 amostras para o exame bacteriológico de animais com quadro de diarreia nos municípios paulistas de São João da Boa Vista, Dourado, Pirassununga, Registro, Pariquera Açu, Pilar do Sul e uma propriedade no estado do Paraná, município de Santana do Itararé. No exame parasitológico, 45,28% (24) foram positivos para Eimeria spp., 26,42% (14) para Strongyloidea e 1,88 (1) para Toxocara vitulorum. No exame bacteriológico, 97,83%, (45) das amostras foram positivas para E. coli, contudo, somente duas foram consideradas patogênicas (E. coli STEC). Em uma amostra (2,17%) isolou-se Klebsiella pneumoniae; já a presença de Salmonella spp. não foi constatada. Para o presente estudo, a presença de endoparasitas foi bastante relevante, principalmente os casos Eimeria spp., sendo a higiene das instalações e falhas de manejo fatores importantes na ocorrência de diarreia em bezerros búfalos no estado de São Paulo.

scholarly journals Prevalence of intestinal parasitic infection in five farms in Holambra, São Paulo, Brazil

1995 ◽  
Vol 37 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Jun Kobayashi ◽  
Hideo Hasegawa ◽  
Archimedes A. Forli ◽  
Nancy F. Nishimura ◽  
Ademar Yamanaka ◽  
...  
Keyword(s):  
Parasitic Infection ◽  
Strongyloides Stercoralis ◽  
Sao Paulo ◽  
São Paulo ◽  
Infection Rates ◽  
Blastocystis Hominis ◽  
Culture Methods ◽  
E Coli ◽  
Parasitological Survey ◽  
Protozoan Species

A parasitological survey was carried out on 222 inhabitants of five farms in Holambra, located 30 km north of Campinas, São Paulo, Brazil, on October 1992. Approximately 70% of the inhabitants were found to be infected with at least one species of intestinal parasite. The positive rates of 6 helminths and 7 protozoan species detected are as follows: 5.4% Ascaris lumbricoides; 8.6% Trichuris trichiura; 19.8% Necator americanus; 10.4% Strongyloides stercoralis; 14% Enterobius vermicularis; 0.9% Hymenolepis nana; 3.2% Entamoeba histolytica; 2.7% E. hartmanni; 9.9% E. coli; 14.0% Endolimax nana; 2.3% Iodamoeba butschlii; 10.4% Giardia lamblia; 37.8% Blastocystis hominis. The positive rates of helminth infection were generaly higher in the younger-group under 16 years-old than those in the elder group aged 16 or more, whereas the infection rates of protozoan species were higher in the elder group. The infection rate of Strongyloides was found to be 10.4% by a newly developed sensitive method (an agarplate culture methods).

scholarly journals Pesquisa de enterobacteriáceas e enterovírus em crianças normais e com quadros diarréicos agudos

1968 ◽  
Vol 2 (2) ◽  
pp. 194-206 ◽  
Author(s):  
José Alberto N. Candeias ◽  
Sebastião Timo Iaria ◽  
Dacio de Almeida Christovão ◽  
Ary Walter Schmid ◽  
Augusto de Escragnolle Taunay ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sao Paulo ◽  
São Paulo ◽  
E Coli

Num grupo de 263 crianças de 0 a 24 meses de idade, atendidas de outubro de 1963 a setembro de 1964 no Centro de Saúde da Lapa, na Capital de São Paulo, foram feitos exames de fezes para a pesquisa de Escherichia coli, do grupo da gastroenterite infantil, Shigella, Salmonella, poliovírus, vírus Coxsackie e vírus ECHO. O agente mais freqüentemente isolado foi o vírus da poliomielite (15,97% de positividade), seguindo-se-lhe a Escherichia coli G.E.I. (10,65%), Shigella (9,51%), vírus Coxsackie (7,22%), Salmonella em 3,04% dos casos e vírus ECHO no mesmo percentual. Em 16,22% das 111 que apresentaram resultado positivo, o exame revelou a presença de dois ou mais agentes. Os agentes acima referidos foram isolados de 42,21% da totalidade das crianças examinadas. Dentre as 167 que apresentavam diarréia, a porcentagem de positividade chegou a 47,90%, e nas 96 que nao a referiam, desceu a 32,29%. Sòmente em relação às shigelas foi possível evidenciar associação entre isolamento de microrganismos e quadro diarréico. Para a E. coli 0111, salmonelas e vírus Coxsackie A, os resultados foram apenas sugestivos desta associação, e para os demais agentes não houve evidência alguma favorável à mesma.

scholarly journals Bactérias gram-negativas em cardeais (Paroaria coronata e Paroaria dominicana) apreendidos do tráfico de animais silvestres

2016 ◽  
Vol 53 (1) ◽  
pp. 107 ◽  
Author(s):  
Marcos Paulo Vieira Cunha ◽  
Marta Brito Guimarães ◽  
Yamê Miniero Davies ◽  
Liliane Milanelo ◽  
Terezinha Knöbl
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Sao Paulo ◽  
São Paulo ◽  
Salmonella Spp ◽  
E Coli

Anualmente o tráfico de animais silvestres retira milhões de aves da natureza. Os cardeais (Paroaria coronata) e cardeais-do-nordeste (Paroaria dominicana) estão incluídos entre as espécies de aves mais traficadas. A microbiota cloacal de passeriformes de vida livre é composta principalmente por bacilos e cocos gram-positivos, já os bacilos gram-negativos predominam em aves de cativeiro. Em situações de estresse e baixa de imunidade as bactérias gram-negativas podem causar infecções oportunistas. O presente trabalho identificou bactérias da microbiota da cloaca de 49 espécimes de P. coronata e P. dominicana apreendidas do tráfico de animais silvestres em São Paulo (SP). Foram isoladas treze espécies de bactérias gram-negativas, incluindo Salmonella spp. e Pseudomonas aeruginosa. A maior frequência de ocorrência foi de Escherichia coli, identificada em 42/49 (85,7%) das amostras fecais. Dentre os isolados de E. coli, 21/42 pertenciam aos grupos filogenéticos B2 e D, relacionados a estirpes patogênicas que causam doença extraintestinal em humanos. Klebsiella pneumoniae foi isolada em 28/49 (57,1%) das amostras. Esses resultados reforçam que as condições estressantes a que esses animais são submetidos em situações de tráfico, incluindo o contato com humanos, podem favorecer a colonização da microbiota cloacal das aves por patógenos, o que representa um risco para a sua reintrodução na natureza considerando-se o possível contato com humanos e outros animais.

Lignicolous marine fungi from São Paulo, Brazil

1983 ◽  
Vol 61 (2) ◽  
pp. 488-506 ◽  
Author(s):  
Tom Booth
Keyword(s):  
Marine Fungi ◽  
Culture Media ◽  
Sao Paulo ◽  
Morphological Features ◽  
São Paulo ◽  
Paulo State ◽  
São Paulo State ◽  
Fungi Imperfecti ◽  
Collection Sites

Lignicolous substrates, i.e., wood and leaves, were collected from 10 São Paulo State locations and 2 Pernambuco State sites. These were subsequently surveyed and incubated and (or) washed, macerated, and plated on culture media. Six types of wood and mangrove leaves were placed in exposed and buried digestors, recovered, surveyed, incubated, and resurveyed for marine fungi. Of a total of 69 isolated taxa, 32 species, including 24 ascomycetes, seven fungi imperfecti, and one basidiomycete, are discussed. Included are Ceriosporopsis cambrensis; C. halima; Corollospora lacera; C. maritima; C. pulchella; C. trifurcata; Crinigera maritima; Didymosphaeria enalia; Haligena viscidula; Halosarpheia fibrosa; Halosphaeria appendiculuta; H. cucullata; H. galerita; H. hamata; H. maritima; H. mediosetigea; H. quadricornuta; H. salina; Leptosphaeria cfr. marina, Leptosphaeria sp.; Lignincola laevis; Lulworthia medusa (f. grandispora); Mycosphaerella sp.; Torpedospora radiata; Ceriosporopsis halima anamorphic form; Cirrenalia tropicalis; Clavariopsis bulbosa; Periconia prolifica; Scolecobasidium? arenarium; Septoria sp.; Trichocladium achrasporum; and Nia vibrissa. Morphological features, collection sites, substrates, slides, cultures, taxonomic deposition, and ecological considerations are presented for each species. A possible Scolecobasidium–Septoria–Mycosphaerella anamorphic–teleomorphic association is suggested.

Checklist of decapods (Crustacea) from the coast of the São Paulo state (Brazil) supported by integrative molecular and morphological data: I. Infraorder Caridea: families Hippolytidae, Lysmatidae, Ogyrididae, Processidae and Thoridae

Zootaxa ◽  
2018 ◽  
Vol 4370 (1) ◽  
pp. 76 ◽  
Author(s):  
MARIANA TEROSSI ◽  
ALEXANDRE O. ALMEIDA ◽  
RAQUEL C. BURANELLI ◽  
ANTONIO L. CASTILHO ◽  
ROGÉRIO C. COSTA ◽  
...  
Keyword(s):  
First Record ◽  
Molecular Data ◽  
Color Pattern ◽  
Sao Paulo ◽  
South Atlantic ◽  
Molecular Techniques ◽  
Morphological Data ◽  
São Paulo ◽  
Paulo State ◽  
São Paulo State

The current checklist is the result of a long-term multidisciplinary project which combined molecular techniques (mitochondrial DNA markers) and morphological analyses of adult specimens for an accurate and detailed identification of the total biodiversity of decapod crustaceans from marine and coastal (including estuaries) environments of São Paulo State (Brazil). This is the first of a series of reports and providing a checklist of caridean shrimps of the families Hippolytidae (5 spp.), Lysmatidae (6 spp.), Ogyrididae (2 spp.), Processidae (5 spp.) and Thoridae (1 sp.). We collected material of 13 species out of 19 recorded, with sequences of cytochrome oxidase subunit I - barcode region and 16S generated from 10 species. The previous record of Lysmata cf. intermedia for São Paulo is actually L. jundalini, as the first record in São Paulo/South Atlantic waters. The molecular data were helpful to confirm the identification of some species, as the occurrence of L. wurdemanni which is confirmed in the South Atlantic Ocean based on morphological, color pattern and molecular data. 

Spatial distribution, prevalence and epidemiological risk factors of cysticercosis in cattle from state of São Paulo, Brazil, slaughtered for human consumption

2017 ◽  
Vol 8 ◽  
pp. 117-122
Author(s):  
Gabriel Augusto Marques Rossi ◽  
Welber Daniel Zanetti Lopes ◽  
Henrique Meiroz de Souza Almeida ◽  
Vando Edésio Soares ◽  
Carlos Eduardo Gamero Aguilar ◽  
...  
Keyword(s):  
Risk Factors ◽  
Spatial Distribution ◽  
Sao Paulo ◽  
Human Consumption ◽  
São Paulo ◽  
Epidemiological Risk

Determination of flagellar types by PCR-RFLP analysis of enteropathogenic Escherichia coli (EPEC) and Shiga toxin-producing E. coli (STEC) strains isolated from animals in São Paulo, Brazil

2012 ◽  
Vol 92 (1) ◽  
pp. 18-23 ◽  
Author(s):  
Claudia de Oliveira Ayala ◽  
Ana Carolina Ramos Moreno ◽  
Marina Baquerizo Martinez ◽  
Ylanna Kelner Burgos ◽  
Antonio Fernando Pestana de Castro ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Rflp Analysis ◽  
Sao Paulo ◽  
São Paulo ◽  
Enteropathogenic Escherichia Coli ◽  
E Coli ◽  
Pcr Rflp

Persistent bloody diarrhoea without fever associated with diffusely adherent Escherichia coli in a young child

2013 ◽  
Vol 62 (12) ◽  
pp. 1907-1910 ◽  
Author(s):  
Sandra Patzi-Vargas ◽  
Mussaret Zaidi ◽  
Rodolfo Bernal-Reynaga ◽  
Magda León-Cen ◽  
Alba Michel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Culture Media ◽  
Evidence Base ◽  
Antimicrobial Treatment ◽  
Molecular Techniques ◽  
Bloody Diarrhoea ◽  
Severe Diarrhoea ◽  
E Coli ◽  
Clinical Laboratories

Diffusely adherent Escherichia coli (DAEC) is thought to cause diarrhoea in children, and so too are other diarrhoeagenic E. coli (DEC); however, the evidence base is inconclusive. DEC pathotypes are differentiated on the basis of their pathogenic features, and thus cannot be quickly identified on selective culture media. Molecular techniques, not readily available in most clinical laboratories, are required to differentiate DEC strains from non-pathogenic E. coli in the stool flora. We report a case of persistent bloody diarrhoea, without fever, in a previously healthy 21-month infant from whom we isolated five DAEC strains. The child’s stools movements were loose, with gross blood and mucus; fresh mount analysis revealed numerous faecal leukocytes and erythrocytes. Response to antimicrobial treatment with trimethoprim-sulfamethoxazole was poor despite susceptibility in vitro. Although the patient improved with azithromycin, blood was present in the patient’s stools for over 30 days. The severe diarrhoea in this patient might be explained by the fact that these DAEC isolates harboured a siderophore receptor, which allows the bacteria to use iron derived from haem compounds that promote its multiplication. The isolates also induced in vitro secretion of several immunomodulatory cytokines that may account for the patient’s loose stools and faecal leukocytes. DAEC may play a greater role than suspected in afebrile children with bloody diarrhoea.

Sign in / Sign up

Export Citation Format

Share Document