Pathogenetic Relationship between Endothelial Dysfunction and Disorders of Blood Coagulation Potential in Pregnancy Complicated by Pre-Eclampsia

Until now, there is no systematic information on the role of endothelial dysfunction in the mechanisms of disorders of blood coagulation potential and microcirculation in different organs and tissues in preeclampsia.Objective: Our aim was to extend the existing principles of diagnosis of pre-eclampsia by establishing the role of endothelial dysfunction in the mechanisms of blood coagulation potential violations. Methods: A prospective comparative study was performed. Condition of coagulation processes studied by conventional techniques, parameters of a functional endothelium (nitric oxide metabolites, endothelin 1, thrombospondin, thrombomodulin and intercellular adhesion molecules in blood plasma) — by ELISA.Results: The study group included 55 patients with moderate preeclampsia and 49 pregnant women with severe pre-eclampsia, in the control group — 40 women with physiological pregnancy. In patients with pre-eclampsia moderate observed increase in plasma endothelin-1 (p 0.001), thrombospondin (p 0.001), intercellular adhesion molecules (p 0.001) while reducing the level of nitrogen oxide (p 0.001), increase in time of fibrinolysis (p 0.050) and decreased international normalized ratio (p 0.050) compared with the control group. With increasing severity of preeclampsia the researchers detected in blood plasma of patients a progressive increase in endothelin 1 (p1 0.020), thrombospondin (p1 0.001), intercellular adhesion molecules (p1 0.001) and decrease of nitric oxide metabolites (p1 0.001) and thrombomodulin (p1 0.001); the last combined with the activation of procoagulant hemostasis.Conclusion: There is a pathogenetic relationship between the development of endothelial dysfunction, impaired blood coagulation potential and the severity of clinical signs of preeclampsia. To widen the number of existing techniques to diagnose the severity of pre-eclampsia we recommende to mesure endothelin 1, thrombomodulin, thrombospondin, intercellular adhesion molecules and nitric oxide metabolites in the blood plasma, and use traditional indicators to assess the hemostatic system.

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Influence of Hypoxic and Hyperoxic Preconditioning on Endothelial Function in a Model of Myocardial Ischemia-Reperfusion Injury with Cardiopulmonary Bypass (Experimental Study)

International Journal of Molecular Sciences ◽

10.3390/ijms21155336 ◽

2020 ◽

Vol 21 (15) ◽

pp. 5336

Author(s):

Irina A. Mandel ◽

Yuri K. Podoksenov ◽

Irina V. Suhodolo ◽

Darya A. An ◽

Sergey L. Mikheev ◽

...

Keyword(s):

Nitric Oxide ◽

Cardiopulmonary Bypass ◽

Endothelial Function ◽

Light Microscopy ◽

Reperfusion Injury ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Control Group ◽

Endothelin 1 ◽

Nitric Oxide Metabolites

The aim of the experiment was to evaluate the effect of preconditioning based on changes in inspiratory oxygen fraction on endothelial function in the model of ischemia-reperfusion injury of the myocardium in the condition of cardiopulmonary bypass. The prospective randomized study included 32 rabbits divided into four groups: hypoxic preconditioning, hyperoxic preconditioning, hypoxic-hyperoxic preconditioning, and control group. All animals were anesthetized and mechanically ventilated. We provided preconditioning, then started cardiopulmonary bypass, followed by induced acute myocardial infarction (ischemia 45 min, reperfusion 120 min). We investigated endothelin-1, nitric oxide metabolites, asymmetric dimethylarginine during cardiopulmonary bypass: before ischemia, after ischemia, and after reperfusion. We performed light microscopy of myocardium, kidney, lungs, and gut mucosa. The endothelin-1 level was much higher in the control group than in all preconditioning groups after ischemia. The endothelin-1 even further increased after reperfusion. The total concentration of nitric oxide metabolites was significantly higher after all types of preconditioning compared with the control group. The light microscopy of the myocardium and other organs revealed a diminished damage extent in the hypoxic-hyperoxic preconditioning group as compared to the control group. Hypoxic-hyperoxic preconditioning helps to maintain the balance of nitric oxide metabolites, reduces endothelin-1 hyperproduction, and enforces organ protection.

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Level of Nitrogen Oxide and Indicators of Oxidative-Antioxidative Status in the Gingival Tissues, Blood Plasma in Experimental Chronic Gastritis and Duodenitis and Drug Correction

Journal of Biomimetics Biomaterials and Biomedical Engineering ◽

10.4028/www.scientific.net/jbbbe.31.83 ◽

2017 ◽

Vol 31 ◽

pp. 83-90

Author(s):

Y.G. Romanenko

Keyword(s):

Nitric Oxide ◽

Calcium Carbonate ◽

Blood Plasma ◽

Chronic Gastritis ◽

Gingival Tissue ◽

Control Group ◽

Treatment Level ◽

Antioxidative Status ◽

Nitric Oxide Metabolites

model of chronic gastritis and duodenitis was conducted in 48 immature Wistar rats both gender by intragastric injection of medical bile. Control group consisted of 10 animals. After approximation model of the gastritis and duodenitis rats were divided into five subgroups: the 1st subgroup (before treatment, 9 rats), subgroup 2 (10 animals) received the antioxidant, subgroup 3 (11 animals) – received NO donator, subgroup 4 (10 animals) - received an antioxidant and NO donator, subgroup 5 (8 animals) – received the antioxidant, NO donator and calcium carbonate. In rats with gastritis and duodenitis was observed redistribution of stable metabolites of nitric oxide: decreasing level in the gingival homogenates and increasing in the blood plasma. Level of malondialdehyde and aldehyddehydrogenase in the gingival tissue increased, and in the blood plasma decreased, on a background of catalase activation; content of ketone phenilhydrazone remained in the level of control group. Decreasing markers of oxidation lipids and proteins in the blood plasma, on a background of increasing levels of catalase and nitric oxide metabolites (in 14 times), indicates about a key role of NO in the antioxidant protection of organism in a case of disease.Application of antioxidant could not impact on the indicators of oxidation lipids and proteins. It had been shown decreasing content of the nitric oxide metabolites in the gingival tissues and blood plasma. Drug correction with donator of nitric oxide have to increase markers of oxidation lipids and proteins in the gingival tissues, on a background of high catalase activity and low levels of nitric oxide metabolites. In the blood plasma content of nitric oxide metabolites was higher, which indicated about an active inflammatory process in the stomach and duodenum mucosa. Indicators of the protein molecules fragmentation and malondialdehyde were not differ from those before treatment. Level of catalase was in two times higher, than in the control group, but level of superoxide dismutase was decreased. Complex of antioxidant and donator of nitric oxide helped to stabilise the indicators of oxidation lipids and proteins, although level of nitric oxide metabolites in the gingiva was significantly decreased. Application complex from the antioxidant, donator of nitric oxide and calcium carbonate at the treatment of chronic gastritis and duodenitis restored an oxidative-antioxidative status in the blood plasma and gingival tissues, increasing the production of nitric oxide to a level in the control group.

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Clinical significance of the level of intercellular adhesion molecules 1 in patients with Candida stomatitis depending on the ability of Candida spp to foms biofilms

Immunopathology Allergology Infectology ◽

10.14427/jipai.2020.4.52 ◽

2020 ◽

pp. 52-57

Author(s):

D Novikov ◽

A Pozharitskaya ◽

I Karpuk

Keyword(s):

Adhesion Molecules ◽

Oral Mucosa ◽

Biofilm Formation ◽

Oral Fluid ◽

Oral Candidiasis ◽

Intercellular Adhesion ◽

Control Group ◽

Microbiological Method ◽

Candida Spp ◽

Intercellular Adhesion Molecules

Aim: to assess the level of intercellular adhesion molecules 1 (ICAM-1) in the oral fluid of patients with candidal stomatitis, depending on the ability of fungi Candida to form a biofilm. The object of the study were 67 patients with oral mucosa candidiasis and 23 patients of the control group without oral candidiasis. A clinical examination of 90 patients was carried out, smears were taken to confirm the diagnosis by a microbiological method, ELISA to assess the level of ICAM-1 in the oral fluid, to determine the biofilm-forming ability of strains of fungi Candida. According to the results of the study, in 41 (61,2%) patients with candidal stomatitis, strains of fungi Candida had the ability to form a biofilm, and in 26 (38,8%) patients this ability was absent, and in 19 patients (46,3%) it was low ability to biofilm formation, in 21 (51,3%) - moderate ability and in 1 (2,4%) - high. In patients with candidiasis stomatitis, the level of ICAM-1 concentration in saliva was 8,51 ± 0,5 ng / ml, which significantly distinguished it (p <0,001) from the indicator in the control group without oral mucosa candidiasis (4,51 ± 0,32 ng / ml). In patients with candidal stomatitis (n = 67), the level of ICAM-1 in saliva was significantly higher (p <0,01) in the group with biofilm-forming strains than with biofilm-non-forming strains.

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Effect of the cytokine network and role of intercellular adhesion molecules in endothelial dysfunction development in patients with acute myocardial infarction

Practical medicine ◽

10.32000/2072-1757-2018-9-92-96 ◽

2018 ◽

Vol 16 (9) ◽

pp. 92-96

Author(s):

R. I. Sadikova ◽

◽

E. G. Mutalova ◽

Keyword(s):

Myocardial Infarction ◽

Acute Myocardial Infarction ◽

Endothelial Dysfunction ◽

Adhesion Molecules ◽

Intercellular Adhesion ◽

Cytokine Network ◽

Intercellular Adhesion Molecules

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P628Relationship between endothelin-1, intercellular adhesion molecules-1 and lipoproteins in acute myocardial infarction

Cardiovascular Research ◽

10.1093/cvr/cvu098.56 ◽

2014 ◽

Vol 103 (suppl 1) ◽

pp. S114.2-S114

Author(s):

M O Harbar ◽

HV Svitlyk ◽

YO Svitlyk ◽

OM Svitlyk ◽

GV Tshngryan

Keyword(s):

Myocardial Infarction ◽

Acute Myocardial Infarction ◽

Adhesion Molecules ◽

Intercellular Adhesion ◽

Endothelin 1 ◽

Intercellular Adhesion Molecules

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Role of insulin during the development of oligofructose (OF)-induced equine laminitis

Bulletin of the Veterinary Institute in Pulawy ◽

10.1515/bvip-2015-0044 ◽

2015 ◽

Vol 59 (2) ◽

pp. 303-309

Author(s):

Renli Jiang ◽

Li Gao ◽

Guanying Wang ◽

Xinran Li ◽

Yue Li ◽

...

Keyword(s):

Nitric Oxide ◽

Glucose Oxidase ◽

Negative Correlation ◽

Significant Negative Correlation ◽

Control Group ◽

Endothelin 1 ◽

Limulus Amoebocyte Lysate ◽

And Control ◽

Over Time

Abstract Horses (n = 20) were divided into 2 groups: oligofructose (OF)-induced equine laminitis group (group OF; n = 11) which received 10 g/kg b.w. of OF dissolved in 4 L water via nasogastric intubation, and control group (NS; n = 9) which received 4 L of saline. Blood was collected at 4 h intervals over 72 h study period and analysed by ELISA, kinetic limulus amoebocyte lysate assay, and glucose-oxidase methods. The level of insulin changed significantly in horses which received OF (P < 0.01); there was a significant negative correlation between the level of adiponectin and insulin over time. The results suggested that insulin may play an important role in the development of OF-induced equine laminitis by altering the level of endothelin-1 and nitric oxide.

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