scholarly journals Improvement of Quantitative Detection Method of Reaction Rate Between HCl(aq) and Mg(s) - for Simplicity of Manipulation and Reproducibility -

2010 ◽  
Vol 4 (2) ◽  
pp. 74-79
Author(s):  
백종호 ◽  
Dae Hong JEONG
Keyword(s):  
Reaction Rate ◽  
Detection Method ◽  
Quantitative Detection

Alcohol Determination by HPLC with Postcolumn Derivatization Based on the Thiosulfate-catalyzed Reaction of Alcohols and Cerium(IV) and Fluorescence Detection of Cerium(III)

2020 ◽  
Vol 16 ◽  
Author(s):  
Ikko Mikami ◽  
Eri Shibayama ◽  
Kengo Takagi
Keyword(s):  
Detection Limit ◽  
Fluorescence Detection ◽  
Reaction Rate ◽  
Detection Method ◽  
Fluorescence Detector ◽  
Reaction Solution ◽  
Optimum Detection ◽  
Postcolumn Derivatization ◽  
Sensitive Method

Background: Determination of a reducing substance based on the reaction between Ce(IV) and a reducing substance and fluorescence detection of Ce(III) generated has been reported as a selective and sensitive method. However, this method could not be applied to the determination of alcohol due to the low reaction rate of alcohol and Ce(IV). Objective: We found that thiosulfate catalytically enhanced reaction of alcohols (such as, methanol, ethanol, and propanol) and Ce(IV). Utilizing this effect, we developed a new method for the determination of alcohols. Results: In the presence of thiosulfate, an increase in fluorescence intensity was detected by injecting alcohol at concentrations of several millimolar, whereas it was not observed even at the concentration of 10% v/v (2 M for ethanol) in the absence of thiosulfate. The optimum detection conditions were determined to be 4.0 mM Ce(IV) sulfate and 0.50 mM thiosulfate, and the detection limit (S/N = 3) of ethanol under these conditions was 1 mM. In the calibration curves, changes in the slope were observed when the alcohol concentrations were approximately 10–25 mM. Using a thiosulfate solution containing ethanol as the reaction solution, a calibration curve without any change in slope was obtained, although the concentration of ethanol at the detection limit increased. The alcohols in the liquor and fuel were successfully analyzed using the proposed detection method as a postcolumn reaction. Conclusion: This new alcohol detection method using a versatile fluorescence detector can be applied to the postcolumn reaction of HPLC omitting need of time-consuming pretreatment processes.

scholarly journals Development and Application of Quantitative Detection Method for Viral Hemorrhagic Septicemia Virus (VHSV) Genogroup IVa

Viruses ◽  
2014 ◽  
Vol 6 (5) ◽  
pp. 2204-2213 ◽  
Author(s):  
Jong-Oh Kim ◽  
Wi-Sik Kim ◽  
Si-Woo Kim ◽  
Hyun-Ja Han ◽  
Jin Kim ◽  
...  
Keyword(s):  
Detection Method ◽  
Quantitative Detection ◽  
Viral Hemorrhagic Septicemia Virus ◽  
Viral Hemorrhagic Septicemia

Extraction of norovirus based on nanomagnetic beads and establishment of a fluorescence quantitative detection method

2020 ◽  
Vol 10 (9) ◽  
pp. 1463-1469
Author(s):  
Hui Chen ◽  
Kai Liu ◽  
Ziqi Xiao ◽  
Shaoyong Chen ◽  
Hang Liu ◽  
...  
Keyword(s):  
Acute Gastroenteritis ◽  
Detection Method ◽  
Melting Curve ◽  
Reaction System ◽  
Threshold Value ◽  
Quantitative Detection ◽  
Rt Pcr ◽  
Effective Prevention ◽  
Positive Stool ◽  
Pcr Method

Norovirus infection is the main cause of epidemic acute gastroenteritis outbreaks. As a result, norovirus merits careful attention. Acute gastroenteritis caused by norovirus is very harmful, necessitating effective prevention and monitoring. This study extracted norovirus using the Fe3O4 nanomagnetic bead method while establishing a rapid and sensitive quantitative norovirus detection method for emergency use. Norovirus RNA was extracted from norovirus-positive stool samples, norovirus-specific primers were designed, and SYBR Green I fluorescent dye was used to construct specific fluorescence quantitative reverse-transcription polymerase chain reaction (RT-PCR) method. Norovirus in the sample was successfully detected with the quantitative RT-PCR method established in this study, with a measured cycle threshold value of 28.73; the melting temperature value of the PCR product was approximately 83.5 °C. Additionally, by analyzing melting curve with quantitative PCR and evaluating the repeatability of the detection method, the performance of the reaction system in terms of specificity and repeatability was determined to be good. The fluorescence quantitative detection method established here can rapidly and effectively detect norovirus, and the method has good specificity and high repeatability. Currently, there is a trend of norovirus infection becoming increasingly severe. Thus, this rapid and sensitive fluorescence quantitative detection method will play a significant role in the monitoring and detection of norovirus and is thus worthy of promotion and application.

scholarly journals Study on the Degradation Effect of Plant-Derived Active Ingredients on Organophosphorus Pesticides

2020 ◽  
Vol 1 (1) ◽  
pp. 6
Author(s):  
Jun Sun ◽  
Tongxin Zhang
Keyword(s):  
Measurement Method ◽  
Detection Method ◽  
Organic Phosphorus ◽  
Organophosphorus Pesticides ◽  
Quantitative Detection ◽  
Active Ingredients ◽  
Degradation Rates ◽  
Before And After ◽  
Concentration Changes ◽  
Degradation Effect

In order to explore new ways and methods for the degradation of organophosphorus pesticides, the degradation effects of plant-derived active ingredients on three organophosphorus pesticides were studied. Mix Rhubarb, Pittosporum bark, Hibiscus bark, and Chinese gall in 9:4:3:2 parts by mass, crush and soak in water, use GC-MS quantitative detection method, rapid pesticide residue measurement method, and compare the organic phosphorus before and after the test Pesticide concentration changes, clarify its degradation effect on organophosphorus pesticides. The results showed that the degradation rates of chlorpyrifos and parathion were 93.2% and 92.9% in the extract within 2 minutes; the degradation rate of dichlorvos in the extract within 17 hours was 66.67%, and the degradation of chlorpyrifos within 11 hours the rate is 48.69%. This study shows that the extracts of rhubarb, sea tongs bark, hibiscus bark, and gallnut have significant degradation effects on chlorpyrifos, parathion, dichlorvos and other organophosphorus pesticides.

scholarly journals The Multiple Infections of Porcine Diarrhea Viruses in Local Area Based on A Luminex xTAG Multiplex Detection Method

2020 ◽  
Author(s):  
Huili liu ◽  
Ying Shi ◽  
Benqiang Li ◽  
Jie Tao ◽  
Jinghua Cheng
Keyword(s):  
Detection Method ◽  
Southern China ◽  
Local Area ◽  
Clinical Samples ◽  
Multiple Infections ◽  
Quantitative Detection ◽  
Multiplex Detection ◽  
Viral Diarrhea ◽  
Infection Pattern ◽  
Pcr Method

Abstract Background Porcine viral diarrhea can cause great damage to the pig industry. However, multiple infections have contributed to the poor control of diarrhea, which has also resulted in great difficulties in determining the main pathogenic factors. Methods A Luminex xTAG multiplex detection method was developed for the detection of 11 viral diarrhea pathogens, which allows for the simultaneous qualitative and quantitative detection of viral diarrhea pathogens in clinical samples. A total of 518 porcine stool specimens were collected from 9 pig herds in Shanghai, China from 2015 to 2017, and the pathogen spectrums and co-infections were analyzed. Results The minimum detection rate of the Luminex xTAG multiplex detection method was at least 10 times higher than the traditional PCR method. Of the 518 diarrhea samples, PEDV was found in 17.57% (91/518), PKoV in 40.35% (209/518), PAstV in 26.64% (138/518), PSV in 15.06% (78/518), PoSaV in 13.13% (68/518), PTV in 5.21% (27/518), PDCoV in 4.83% (25/518), PoRV in 3.28% (17/518), TGEV in 3.09% (16/518), PToV in 1.93% (10/518), and BVDV in 1.74% (9/518), respectively. Furthermore, multiple infections were commonly seen, with positive rate of 35.14%. Infection pattern of the viral diarrheal pathogens in a specific farm was changing, and different farms had the various diarrhea infection patterns. A longitudinal investigation showed that PEDV was still the key pathogen which was closely related to the death of diarrhea piglets. Other pathogens might play synergistic roles in the pathogenesis of diarrhea disease. Conclusions Here we provided a Luminex xTAG multiple detection method for viral diarrhea pathogen infection in clinical, which was more sensitive and specific than general multiplex PCR method. Furthermore, the surveillance confirmed high infection rate of PKoV, but PEDV was still the key pathogen and multiple pathogens synergistically complicated the infection status in southern China, suggesting that controlling porcine diarrhea might be more complex than previously thought. A better understanding of viruses that cause diarrhea in piglets will aid in better preventing and controlling epidemics of viral porcine diarrhea.

scholarly journals Epidemiological analysis of Porcine Viral Diarrhea Pathogens in Local Area

2020 ◽  
Author(s):  
Ying Shi ◽  
Benqiang Li ◽  
Jie Tao ◽  
Jinghua Cheng ◽  
Huili liu
Keyword(s):  
Prevention And Control ◽  
Detection Method ◽  
Multiple Infection ◽  
Clinical Samples ◽  
Quantitative Detection ◽  
Multiplex Detection ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Pcr Method ◽  
And Control

Abstract Background Porcine viral diarrhea can cause great damage to the pig industry and high mortality to piglets. Furthermore, multiple pathogen infections and synergistic infections commonly existed in clinic. This has resulted in great difficulties in determining the main pathogenic factors, which would delay the prevention and control of diseases. Methods A total of 518 porcine stool specimens were collected from 9 pig herds in Shanghai, China from 2015 to 2017 and used for pathogen detection. A Luminex xTAG multiplex detection method was developed for the detection of 11 viral diarrhea pathogens, which allows for the simultaneous qualitative and quantitative detection of viral diarrhea pathogens in clinical samples. Results The minimum detection rate of the Luminex xTAG multiplex detection method was at least 10 times higher than the traditional PCR method. As a result, 209 (40.3%) were positive for porcine kobuvirus (PKoV), 138 (26.6%) for porcine astrovirus (PAstV), 91 (17.6%) for porcine epidemic diarrhea virus (PEDV), 78 (15.1%) for porcine sapelovirus (PSV), 68 (13.1%) for porcine sapovirus (PoSaV), 27 (5.2%) for porcine teschovirus (PTV), 25 (4.8%) for porcine deltacoronavirus (PDCoV), 17 (3.3%) for porcine rotavirus (PoRV), 16 (3.1%) for transmissible gastroenteritis virus (TGEV), 10 (1.9%) for porcine torovirus (PToV), and 9 (1.7%) for bovine viral diarrhea virus (BVDV), respectively. Furthermore, multiple infection rate of diarrhea sample was 17.57% for dual-infection, 11.58%for triple-infection, 4.63% for quadruple-infection, 0.77% for quintuple-infection, 0.58% for sextuple-infection and septuple-infection, respectively. Infection pattern of the viral diarrheal pathogens was changing, and different farm had the various diarrhea infection patterns, which proved the great importance of epidemiological surveillance and the guidance effect to clinical production. PoSaV, PoRV, PAstV, PToV and PEDV were indicated as the predominant viruses of clinical samples collected in 2017 by the quantitative analysis. Conclusions Here we provide a Luminex xTAG multiple detection method for viral diarrhea pathogen infection in clinical,which was more sensitive and specific than general multiplex PCR method. Furthermore, the surveillance confirmed the complicated infection status in China, which demonstrated the need for continuous surveillance and provided data for the prevention and control of viral diarrhea.

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