scholarly journals Effect of PEG and mannitol to induce drought stress on seed quality and stand establishment in wheat (Triticum aestivum L.) genotypes

2021 ◽  
Vol 17 (2) ◽  
pp. 469-475
Author(s):  
S. B. Patil ◽  
Jitendra Kumar S. Hilli

An experiment was conducted to study the screening of wheat (Triticum aestivum L.) genotypes for drought tolerance under laboratory condition during2018 at Department of Seed Science and Technology, College of Agriculture, Vijayapura. The experiment was carried out in a factorial Complete Randomized Block Design with seven treatments replicated three times. Twenty six wheat genotypes were screened for drought tolerance using 0, 15, 30, 60 per cent polyethylene ethylene glycol 6000 (PEG6000) and 1, 2, 5 per cent mannitol solutions. Data were recorded on various seedling parameters like germination percentage, root length, shoot length, seedling dry weight and seedling vigour index. The seedling traits showed a decreasing trend in response to increased concentrations of PEG6000 and mannitol. Wheat genotype DBW-110 (98.70 %) and C-306 (92.50 %) were found to be the best genotype for screened based on germination percentage at 15 per cent PEG6000 and 5 per cent mannitol. Wheat genotypes HI-1620 (6.7 cm) and NIAW-3212 (4.5 cm) produced maximum root length, respectively at higher concentrations of PEG6000 and mannitol. The genotype HI-1620 (6.4 cm, @ 15 %; 5.4 cm, @ 5 % mannitol) showed maximum shoot length. The genotype HD-2733, DBW-14, DBW-88 showed highest reduction of seedling parameters observed at increase in osmotic stress condition.

2021 ◽  
Vol 13 (2) ◽  
pp. 735-743
Author(s):  
Umesh Hiremath ◽  
Basave Gowda ◽  
Lokesh G.Y . ◽  
B. S. Ganiger

The continuous rise in global population demands more food production under limited land resources accompanied by climate change. More oversupply of quality seeds to meet ever-growing food demand is a highly challenging task. Seeds with low vigour level limit the growth and yield of crops at varied field conditions. Hence, an experiment was conducted to develop a technique for the fast and homogeneous growth of kabuli chickpea seeds. The priming treatments evaluated in the present study enhanced the various seed quality parameters of kabuli chickpea significantly when compared to control. Hydro-priming for 4 hours (at 20°C) and air drying for 72 hours showed better results compared to rest of the treatments both in fresh seeds which recorded higher first count (66.38%), germination (92.50%) root length (11.85 cm), shoot length (6.16), mean seedling length (18.01), dry seedling weight (747 mg), seedling vigour index-I (1665), higher speed of emergence (0.3992) and old seeds with higher first count (61.88%), germination (74.13%) root length (8.15 cm), shoot length (5.85 cm), mean seedling length (14.00 cm), seedling dry weight (611 mg), seedling vigour index-I (1056), higher speed of emergence (0.3814). The aged seeds have recorded 29 % increase in germination percentage in contrast to 8 percent increases in fresh seeds. Kabuli chickpea seeds could be subjected to hydropriming, an affordable, alternative, and eco-friendly technique for improving seed and seedling vigour of kabuli chickpea.


2016 ◽  
Vol 8 (2) ◽  
pp. 971-980
Author(s):  
S. S. Patra ◽  
B. Mehera ◽  
S. Rout ◽  
S. S. Tomar ◽  
M. Singh ◽  
...  

The present investigation was conducted to study the effects of hydropriming and different sowing dates on growth and yield attributes of wheat (Triticum aestivum L.) during Rabi season of 2014-15.The experiment was conducted in Randomised Block Design with three replications. The highest germination percentage was recorded at T4 [hydropriming] 22nd Nov + 16 hrs. ( 94.04%), plant height highest was recorded at T4 (95.23cm), highest number of tillers at T4 (4.40), number of spikelet per spike highest at T4 (18.73), numbers of grains per spike highest at T4 (53.13), root length (16.07cm), test weight (43.33g), grain yield (42.79 q/ha), harvest index (63.46%) recorded similar result in same treatment. Therefore it may be concluded that 22nd Nov with 16 hrs. of hydropriming treatment can be recommended to PBW-343 wheat grower for obtaining better growth and yield.


Author(s):  
P. Masilamani ◽  
T. Eevera ◽  
T. Ramesh ◽  
S. Venkatesan

Background: The present investigation is an attempt to study the effect of different harvesting and threshing methods on germination and seedling vigour of dhaincha. The use of a combine harvester to harvest dhaincha will be an effective alternative method that has not been widely tried. However, work on different methods of harvesting and threshing of dhaincha is limited. Hence, a study was conducted to evaluate the impact of various harvesting and threshing methods on germination and seedling vigour of dhaincha. Methods: This study was conducted at Anbil Dharmalingam Agricultural College and Research Institute, TNAU, Tiruchirappalli, Tamil Nadu. The dhaincha crop was harvested and threshed using four different methods viz., manual harvesting and manual threshing, manual harvesting and threshing by tractor treading, manual harvesting and mechanical threshing and harvesting and threshing by combine harvester. The resultant seeds were tested for mechanical damage and germination potential. The experiment was laid in completely randomized block design. Germination was tested by roll towel method using 100 seeds in four replications. Germination percentage, root and shoot length were measured in seven days after sowing from ten randomly selected seedlings in each replication. For the estimation of dry matter production, ten seedlings were selected at random and kept in a hot air oven maintained at 85oC for 24 hours after measuring their root and shoot length and vigour index was calculated. Mechanical damage to seeds was observed by ferric chloride test. Seed recovery per cent was calculated based on the 100 kgs of dhaincha seeds were cleaned and graded treatment wise using cleaner cum grader and the seeds retained on the bottom sieve were weighed and expressed as per cent of total quantity of seed. Result: The results revealed that the significant difference was found among the different harvesting and threshing methods. The seeds harvested and threshed by manual method recorded 85 per cent germination followed by seeds harvested manually and threshed by mechanical threshing (84 per cent) and seeds harvested manually and threshed by tractor treading (80.5 per cent). The lowest germination of 80.0 per cent was recorded by combine harvesting. From this study, it could be inferred that combine harvester is a modern method for harvesting of dhaincha that saves time and labour when compared to all other methods.


2017 ◽  
Vol 9 (3) ◽  
pp. 1740-1743
Author(s):  
S. P. Monalisa ◽  
J. K. Beura ◽  
R. K. Tarai ◽  
M. Naik

An experiment on seed quality enhancement of common bean (Phaseolus vulgaris L.) var. S 9 (local) was conducted at the department of seed science and technology, OUAT, Bhubaneswar during 2013-14 by use of three biocontrol agents viz. Trichoderma viride, Trichoderma harzianum, Pseudomonas fluorescence. Seeds were bi-oprimed with the biocontrol agents at 40, 50 and 60 % concentration for 4,8,12 and 16 hours of soaking. Seeds were also hydro primed for 4,8,12 and 16 hours. Unprimed dry seed resulted in germination (69 %), shoot length (27.5 cm), root length (14 cm), seedling dry weight (1.71g), SVI-I (2859.2), SVI-II (118.0) and speed of germination (5.8) while hydro primed seeds resulted in germination (72%), shoot length (31.9 cm), root length (15 cm), seedling dry weight (1.80 g), SVI-1 (3375.9) SVI-II (129.8) and speed of germination (6.7). Trichoderma harzianum at 40% con-centration and for 4 hours of soaking resulted enhancement of above quality parameter like 13.0 % in germination, 21.1 % in shoot length, 20.7 % in root length, 31.6 % in seedling dry weight, 36 % in seedling vigour index-I, 48.1 % in seedling vigour index-II and 58.6 % in speed of germination over unprimed seeds. Bio priming with P. fluorescence ( at 40% concentration and for 4 hour) closely followed and at par with best treatment with 11.6 %, 18.2 %, 16.4 %, 30.4 %, 30.7 % and 56.9 % enhancement of above mentioned quality parameters, respectively.


1970 ◽  
Vol 44 (3) ◽  
pp. 303-310
Author(s):  
A Khatun ◽  
MAH Bhuiyan ◽  
MM Ud-deen

Laboratory experiments were carried out to determine the effects of harvesting stages on the seed quality of chickpea. Chickpea seed of three varieties (BARI Chola-5, BARI Chola-6 and BARI Chola-8) were collected at three harvestings times i.e. from three different times at 7 days interval prior to harvesting, viz. i) when the pods were yellowish with a few yellow greens (H1 stage), ii) when most of the pods were light brown with a few yellow (H2 stage), and iii) when all the pods were completely brown and dry (H3 stage). Significant variation was observed in three varieties of chickpea for all the parameters studied except vigour-I. The highest germination percentage, root length, shoot length, root plus shoot length and vigour-II was observed in BARI Chola-5 which was significantly higher over BARI Chola-8. Dry weight was found to be significantly higher in BARI Chola-8 which was identical to BARI Chola-6. Moisture content was significantly higher in BARI Chola-8. Seed collected at the stage when most of the pods were light brown with a few yellow (H2 stage) recorded the highest germination percentage, dry weight, vigour-I and root length. Pods (H1 stage) gave the highest moisture content in seeds. BARI Chola-5 seeds recorded the highest germination at H2 stage. Interaction effects of varieties and harvesting stage had non-significant effect on germination percentage, vigour-I, shoot length and vigour-II in both the years. Keywords: Chickpea, Harvesting stage, Storage, Vigour, Moisture, Germination DOI: 10.3329/bjsir.v44i3.4403 Bangladesh J. Sci. Ind. Res. 44(3),303-310, 2009  


2021 ◽  
Vol 23 (2) ◽  
pp. 51-58
Author(s):  
MAT Sohel ◽  
MAE Hossain ◽  
HP Roy ◽  
SM Reza ◽  
FH Shanta ◽  
...  

The experiment was carried out at the research field of Agronomy and Farming Systems Division, Bangladesh Sugarcrop Research Institute (BSRI), Ishurdi, Pabna during 2012-2013 to determine the most suitable spacing for sugarbeet cultivation in Bangladesh. The experiment was conducted with nine spacing viz. 50 cm × 20 cm, 60 cm × 20 cm, 70 cm × 20 cm, 50 cm × 25 cm, 60 cm × 25 cm, 70 cm × 25 cm, 50 cm × 30 cm, 60 cm × 30 cm and 70 cm × 30 cm in a randomized complete block design with three replications. The effects of spacing on sugarbeet plantation were observed on growth and growth contributing components (germination percentage, number of leaves plant-1, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, shoot dry weight, crop growth rate) of sugarbeet. The highest germination percentage (95.67%), number of leaves plant-1 (34.33) at 30 DAS, shoot length (54.07 cm) at 120 DAS, root fresh weight (969.47 g plant-1) at 150 DAS, shoot fresh weight (752.47 g plant-1) at 120 DAS and other growth contributing parameters were obtained with the spacing 70 cm × 30 cm. However, the maximum root length (38.97 cm) was obtained with 50 cm × 20 cm spacing. It was concluded that the wider spacing promoted the growth of individual beet, though the optimum spacing for maximum root growth of sugarbeet was 50 cm × 20 cm. Bangladesh Agron. J. 2020, 23(2): 51-58


2017 ◽  
Vol 9 (4) ◽  
pp. 2338-2343
Author(s):  
Arpana D. Vaja ◽  
J. B. Patel ◽  
R.N. Daki ◽  
Shital A. Chauhan

The present investigation on effect of nitrogen [N0 (Control), N1 (50 kg N/ha), N2 (100 kg N/ha), and N3 (150 kg N/ ha)] and plant growth regulators [G0 (Control), G1 (50 ppm GA3), G2 (50 ppm NAA) and G3 (500 ppm Cycocel)] on seed yield per plant and seed quality parameters in brinjal (Solanum melongena L.) cv. GJB 3 was carried out at the Instructional Farm, Department of Agronomy, Junagadh Agricultural University, Junagadh during kharif 2015-16. The experiment was laid out in field as per randomized block design (Factorial) with three replications. The seed harvested from 16 different treatments combinations replicated thrice from the field were analyzed in the laboratory following completely randomized design (factorial) for various seed quality parameters. Application of nitrogen @ 150 kg N/ha recorded significantly (P<0.05) highest seed yield per plant, shoot fresh weight, root dry weight, shoot dry weight and vigour index – 2 (mass), while application of 100 kg N/ha resulted in significantly (P<0.05) highest germination percentage, root length, shoot length, root fresh weight and vigour index – 1 (length). Application of GA3 at 50 ppm recorded significantly the highest seed yield per plant, germination percentage, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, shoot dry weight, vigour index – 1 (length) and vigour index – 2 (mass). Among the 16 different treatment combinations, nitrogen @ 150 kg/ha and GA3 50 ppm noted the maximum seed yield per plant, shoot dry weight and vigour index – 2 (mass), while significantly the maxi-mum germination percentage, root length, shoot length, root fresh weight, shoot fresh weight and vigour index – 1 (length) were registered in treatment combination nitrogen 100 kg N/ha and GA3 @ 50 ppm. Therefore, it is advised that application of 100-150 kg of N/ha as a nitrogenous fertilizer and spray GA3 @ 50 ppm (G1) at 45 days after transplanting helps in increasing the seed yield per plant and seed quality parameters in brinjal seed production.


2016 ◽  
Vol 41 (3) ◽  
pp. 451-460 ◽  
Author(s):  
MN Hasan ◽  
MA Salam ◽  
MMI Chowdhury ◽  
M Sultana ◽  
N Islam

The experiment was conducted at the Seed Laboratory, Department of Agronomy, Bangladesh Agricultural University, Mymensingh during the period from September to October, 2011 to study the effect of chemical priming of seed on germination and growth of rice seedling cv. BRRI dhan 29. Seeds were soaked in 3% and 5% solutions of CaCl2, ZnSO4 and KCl for 24, 30, 36, 42, 48, 54, 60 hours, respectively. A control was maintained where seeds were subjected to no priming treatment. The experiment was laid out in a Completely Randomized Design with three replications. Primed seeds were tested for germination and vigour on sandy soil media (60:40) in petridish under field capacity. Seed quality tests included mean germination time, shoot length, shoot dry mass, root length and root dry mass. The results revealed that priming treatments had significant influence on germination and all the growth parameters of rice seedlings. Priming with 3% ZnSO4 for 30 hours showed the highest germination percentage and the lowest mean germinaion time. Priming with 5% KCl for 54 hours showed the highest root length while 5% of the same solution for 24 hours showed the highest root dry mass. On the contrary, seeds having no priming treatment showed the lowest values for germination, vigour index, shoot length,shoot dry mass, root length and root dry mass and the highest mean germination time. The present study concludes that rice seed cv. BRRI dhan29 could be primed with 3% ZnSO4 solution for 30 hours for improving germination and seedling growth.Bangladesh J. Agril. Res. 41(3): 451-460, September 2016


2020 ◽  
Vol 5 (2) ◽  
pp. 66
Author(s):  
Muhammad Kadir ◽  
Kaimuddin Kaimuddin ◽  
Yunus Musa ◽  
Muh Farid Badaruddin ◽  
Amin Nur

Abiotic factors, such as temperature and drought, are the main factors limiting the cultivation under the tropical condition. Two-stage experiments were conducted to examine the drought-tolerant potential of some wheat genotypes against the osmotic stress under the tropical condition at the Laboratory and Greenhouse of Hasanuddin University and Indonesian Cereal Research Institute. The experiments were arranged in a randomized block design with the split-plot pattern and respectively provided with four and three replications. The main plot was potential osmotic stress (0, -0. 33 , and -0.67 MPa) and the sub-plot was selected wheat genotypes (17 genotypes). The results indicates that based on the germination percentage, shoot/root ratio, proline content, stomatal behavior, and relative water content, the wheat lines of O/HP-78-A22-3-7, WBLL*2KURUKU, O/HP-6-A8-2-10, and O/HP-22-A27-1-10 are identified to have better drought-tolerance than the others genotypes based on the analysis of responses to parameters observed. The positively adaptive response of some tropical wheat genotypes to drought stress may be used as a potential donor for further development of drought-tolerant wheat varieties under the tropical climate in Indonesia. 


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