HUMAN CYTOPENIA VARIANTS AT DIVERSE HIV INFECTION STAGES

Over decades, HIV infection and its complications have been one of the most debated problems in the world. The human immunodeficiency virus leads not only to weakened immune system, but also disrupts normal hematopoiesis manifested as cytopenia (anemia, thrombocytopenia and neutropenia). Materials and methods. A retrospective analysis of cases of combined HIV infection and inhibited hematopoiesis was carried out according to hemogram data of patients admitted for treatment at the IKB No. 1 named after D. Dalmatov, Omsk. The inclusion criteria were cytopenia during hospitalization detected in detailed blood test (by calculating hemoglobin level, counts of erythrocytes, leukocytes, platelets). The age of the patients included in the study differed: from 20 to 29 years - 27 patients (24.6%), from 30 to 39 years - 69 subjects (62.7%), from 40 to 49 years - 13 patients (11.8%), over 50 years old 1 patient (0.9%). All patients had suppression of at least one hematopoietic cell lineage. Anemia was considered as decreased hemoglobin level below than 130 g / l in men and 120 g / l in women. Erythrocytopenia was considered as decreased erythrocyte count below 4.76x10 * 12 / l. Leukopenia was defined as decreased total count of leukocytes below 4.0x10 * 9 / L, while a decrease in the absolute count of neutrophils below 1000 cells / μL was considered as neutropenia. Thrombocytopenia was determined as decreased platelet count below 150x10 * 9 \ l. Results. All patients had suppression of at least one hematopoietic cell lineage. 6 patients with stage 2 had one-cell lineage cytopenias, 7 – two- cell lineages. While analyzing the data obtained, it can be concluded that in patients with stage 2 HIV, inhibition of erythroid and platelet cell lineage predominates, whereas thrombocytopenia reached grade IV. At stage 3 HIV, all 7 patients had inhibition of only one cell lineage. In this group, the inhibition of hematopoiesis had a lighter degree in all hematopoietic cell lineages. In 46 patients with stage 4, there were various oppression of one of the hematopoietic cell lineages, in 44 patients there were two-cell lineage cytopenias. For patients with a more advanced stage of HIV, a decrease in the number of all cellular elements of the blood in the hemogram is characteristic; these disorders are more severe and persistent.

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In vitro analysis of epiblast tissue potency for hematopoietic cell differentiation

Development ◽

10.1242/dev.122.3.823 ◽

1996 ◽

Vol 122 (3) ◽

pp. 823-830 ◽

Cited By ~ 2

Author(s):

M. Kanatsu ◽

S.I. Nishikawa

Keyword(s):

Cell Differentiation ◽

Culture System ◽

Cell Sheet ◽

Cell Lineage ◽

Hematopoietic Cell ◽

Morphogenetic Protein ◽

Hematopoietic Cell Lineage ◽

Vitro Analysis ◽

In Vitro Analysis

In murine embryogenesis, all cells that will constitute the embryonic structures originate from the epiblast (primitive ectoderm) tissue, the epithelial cell sheet of the gastrulating embryo. The cells of this tissue are totipotent at the beginning of gastrulation, but at the end of this period are specified to particular cell lineages. Thus, it is likely that during murine gastrulation, the potency of epiblast cells that were originally totipotent becomes restricted as development progresses. However, the mechanisms of this process are unknown. We have investigated this process in vitro, focusing on the hematopoietic cell lineage. To detect the hematogenic potency of the epiblast tissue, we established an in vitro culture system in which the hematopoietic cell differentiation of the epiblast tissue was supported by a stromal cell layer. With this culture system, we investigated the process by which this potency becomes spatially and temporally restricted during gastrulation. The results showed that hematogenic potency resides in the entire epiblast of the early- to mid-gastrulating embryo, but becomes restricted to the posterior half of the epiblast at the headfold stage. Furthermore, we showed that this process is altered by exogenous bone morphogenetic protein-4 (BMP-4) or activin A, which may be mesoderm inducers in Xenopus embryogenesis.

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The proportion of mitoses in different cell lineages changes during short-term culture of normal human bone marrow

Blood ◽

10.1182/blood.v67.5.1240.bloodjournal6751240 ◽

1986 ◽

Vol 67 (5) ◽

pp. 1240-1243

Author(s):

M Keinanen ◽

S Knuutila ◽

CD Bloomfield ◽

E Elonen ◽

A de la Chapelle

Keyword(s):

Bone Marrow ◽

Cell Lineage ◽

Great Majority ◽

Hematopoietic Cell ◽

Human Bone ◽

Human Bone Marrow ◽

Immunofluorescent Staining ◽

Cytoplasmic Staining ◽

Cell Lineages ◽

Mitotic Cells

To determine the hematopoietic cell lineage of mitotic cells in human bone marrow on direct examination and after 24-hour culture, marrow mitoses from four healthy individuals were studied, using a new technique that allows analysis of karyotypes in cells whose cell membrane and cytoplasm have been preserved. Mitoses were identified as being of erythroid lineage by immunofluorescent staining for surface glycophorin A and as being of granulocytic lineage by cytoplasmic staining for Sudan black B. On direct marrow examination without prior culture, the great majority of mitoses (74% to 90%) were of erythroid lineage; only a few (0% to 10%) were granulocytic. After 24-hour culture, the percentage of erythroid mitoses (15% to 40%) decreased, while the percentage of granulocytic mitoses (58% to 87%) increased strikingly. These data indicate that mitotic cells of different hematopoietic cell lineages predominate in marrow at different culture times and offer a plausible explanation for the high frequency of normal karyotypes in acute myeloid leukemia after direct marrow cytogenetic evaluation.

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Expression of 4-Integrin Defines the Earliest Precursor of Hematopoietic Cell Lineage Diverged From Endothelial Cells

Blood ◽

10.1182/blood.v93.4.1168 ◽

1999 ◽

Vol 93 (4) ◽

pp. 1168-1177 ◽

Cited By ~ 65

Author(s):

Minetaro Ogawa ◽

Masami Kizumoto ◽

Satomi Nishikawa ◽

Tetsuhiro Fujimoto ◽

Hiroaki Kodama ◽

...

Keyword(s):

Stem Cells ◽

Endothelial Cells ◽

Cell Population ◽

Embryonic Stem ◽

Cell Lineage ◽

Hematopoietic Cell ◽

Hematopoietic Stem ◽

Hematopoietic Cell Lineage ◽

E Cadherin

Abstract Embryonic stem cells can differentiate in vitro into hematopoietic cells through two intermediate stages; the first being FLK1+ E-cadherin− proximal lateral mesoderm and the second being CD45− VE-cadherin+endothelial cells. To further dissect the CD45−VE-cadherin+ cells, we have examined distribution of 4-integrin on this cell population, because 4-integrin is the molecule expressed on hematopoietic stem cells. During culture of FLK1+ E-cadherin− cells, CD45− VE-cadherin+4-integrin− cells differentiate first, followed by 4-integrin+ cells appearing in both CD45− VE-cadherin+ and CD45−VE-cadherin− cell populations. In the CD45−VE-cadherin+ cell population, 4-integrin+ subset but not 4-integrin− subset had the potential to differentiate to hematopoietic lineage cells, whereas endothelial cell progenitors were present in both subsets. The CD45−VE-cadherin− 4-integrin+ cells also showed hematopoietic potential. Reverse transcription-polymerase chain reaction analyses showed that differential expression of the Gata2 and Myb genes correlated with the potential of the 4-integrin+ cells to give rise to hematopoietic cell differentiation. Hematopoietic CD45−VE-cadherin+ 4-integrin+ cells were also present in the yolk sac and embryonic body proper of 9.5 day postcoitum mouse embryos. Our results suggest that the expression of 4-integrin is a marker of the earliest precursor of hematopoietic cell lineage that was diverged from endothelial progenitors.

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Expression of 4-Integrin Defines the Earliest Precursor of Hematopoietic Cell Lineage Diverged From Endothelial Cells

Blood ◽

10.1182/blood.v93.4.1168.404k12_1168_1177 ◽

1999 ◽

Vol 93 (4) ◽

pp. 1168-1177 ◽

Cited By ~ 4

Author(s):

Minetaro Ogawa ◽

Masami Kizumoto ◽

Satomi Nishikawa ◽

Tetsuhiro Fujimoto ◽

Hiroaki Kodama ◽

...

Keyword(s):

Stem Cells ◽

Endothelial Cells ◽

Cell Population ◽

Embryonic Stem ◽

Cell Lineage ◽

Hematopoietic Cell ◽

Hematopoietic Stem ◽

Hematopoietic Cell Lineage ◽

E Cadherin

Embryonic stem cells can differentiate in vitro into hematopoietic cells through two intermediate stages; the first being FLK1+ E-cadherin− proximal lateral mesoderm and the second being CD45− VE-cadherin+endothelial cells. To further dissect the CD45−VE-cadherin+ cells, we have examined distribution of 4-integrin on this cell population, because 4-integrin is the molecule expressed on hematopoietic stem cells. During culture of FLK1+ E-cadherin− cells, CD45− VE-cadherin+4-integrin− cells differentiate first, followed by 4-integrin+ cells appearing in both CD45− VE-cadherin+ and CD45−VE-cadherin− cell populations. In the CD45−VE-cadherin+ cell population, 4-integrin+ subset but not 4-integrin− subset had the potential to differentiate to hematopoietic lineage cells, whereas endothelial cell progenitors were present in both subsets. The CD45−VE-cadherin− 4-integrin+ cells also showed hematopoietic potential. Reverse transcription-polymerase chain reaction analyses showed that differential expression of the Gata2 and Myb genes correlated with the potential of the 4-integrin+ cells to give rise to hematopoietic cell differentiation. Hematopoietic CD45−VE-cadherin+ 4-integrin+ cells were also present in the yolk sac and embryonic body proper of 9.5 day postcoitum mouse embryos. Our results suggest that the expression of 4-integrin is a marker of the earliest precursor of hematopoietic cell lineage that was diverged from endothelial progenitors.

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Serum biomarkers discovery of steroid-induced femoral head osteonecrosis in Chinese female by comparative proteome

10.21203/rs.3.rs-17162/v1 ◽

2020 ◽

Author(s):

Ping Zeng ◽

Chongxing Zhang ◽

Yanqiong Zhou ◽

Jinyi Li ◽

Tao Li ◽

...

Keyword(s):

Femoral Head ◽

Healthy Volunteers ◽

Differential Expression ◽

Western Blotting ◽

Serum Proteins ◽

Cell Lineage ◽

Hematopoietic Cell ◽

Diagnostic Biomarkers ◽

Fat Digestion ◽

Hematopoietic Cell Lineage

Abstract Background Steroid-induced osteonecrosis of the femoral head (SONFH) is a disabling, aseptic and ischemic disease due to excessive glucocorticoids (GCs) usage. Patients with SONFH are commonly asymptomatic, which makes its early diagnosis is challenge, the pathological mechanisms of SONFH are not well-known, the purpose of the present study was to screen diagnostic biomarkers for SONFH. Methods The differential expression of serum proteins from SONFH, traumatic osteonecrosis of the femoral head (TONFH) patients and healthy volunteers (CK) in Chinese females was compared using iTRAQ, and potential diagnostic biomarkers were verified by western blotting. Results Gene Ontology, Kyoto Encyclopedia of Genes and Genomes (KEGG), Domain and Clusters of Orthologous Groups (COG) analyses revealed key groups of proteins, pathways and domains differentially regulated among SONFH, TONFH and healthy volunteers in Chinese female, the results showed that peptidase S1, fibrinogen, transferrin, lipid transport domains and hematopoietic cell lineage, fat digestion, absorption, peroxisome proliferator-activated receptor (PPAR) pathways were associated with the development of SONFH. Finally, C-reactive protein (CRP), serum amyloid A protein (SAA1), alpha-1-acid glycoprotein 1 (ORM1) and dopamine beta-hydroxylase were selected for verification of differential expression using western blotting. Conclusions Our data suggest that dysfunction of hematopoietic cell lineage, adhesion, fat digestion and absorption, PPAR pathways may be involved in the pathogenesis of SONFH, serum proteins SAA1, ORM1 could be used as new potential diagnostic biomarkers for SONFH.

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Optimization of equine infectious anemia derived vectors for hematopoietic cell lineage gene transfer

Gene Therapy ◽

10.1038/sj.gt.3302350 ◽

2004 ◽

Vol 12 (1) ◽

pp. 22-29 ◽

Cited By ~ 11

Author(s):

J P O'Rourke ◽

J C Olsen ◽

B A Bunnell

Keyword(s):

Gene Transfer ◽

Cell Lineage ◽

Hematopoietic Cell ◽

Equine Infectious Anemia ◽

Hematopoietic Cell Lineage

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Prediktor Mortalitas Pasien HIV/AIDS Rawat Inap

Jurnal Penyakit Dalam Indonesia ◽

10.7454/jpdi.v3i1.29 ◽

2016 ◽

Vol 3 (1) ◽

pp. 22

Author(s):

Estie Puspitasari ◽

Evy Yunihastuti ◽

Iris Rengganis ◽

Cleopas Martin Rumende

Keyword(s):

Hospital Mortality ◽

Immune Deficiency Syndrome ◽

Deficiency Syndrome ◽

Hemoglobin Level ◽

Chi Square ◽

Predictors Of Mortality ◽

Stage 4 ◽

Immunodeficiency Virus ◽

Acquired Immune Deficiency ◽

Hiv Aids

Pendahuluan. Human Immunodeficiency Virus/Acquired Immune Deficiency Syndrome (HIV/AIDS) adalah masalah besar yang mengancam Indonesia dan banyak negara di seluruh dunia. Pengetahuan tentang karakteristik dan prediktor mortalitas dapat membantu dalam penatalaksanaan pasien. Penelitian ini bertujuan untuk mengetahui faktor-faktor prediktor mortalitas pasien HIV/AIDS dewasa yang dirawat di RSUPN Dr. Cipto Mangunkusumo (RSCM).Metode. Penelitian ini merupakan studi kohort retrospektif pada pasien rawat inap dewasa RSCM yang didiagnosis HIV/ AIDS selama tahun 2011-2013. Data klinis dan laboratorium beserta status luaran (meninggal atau hidup) dan penyebab mortalitas selama perawatan diperoleh dari rekam medis. Analisis bivariat menggunakan uji Chi-Square dilakukan pada tujuh variabel prognostik, yaitu jenis kelamin laki-laki, tidak dari rumah sakit rujukan, tidak pernah/putus terapi antiretroviral (ARV), stadium klinis WHO 4, kadar hemoglobin <10 g/dL, kadar eGFR <60 mL/min/1,73 m2 dan kadar CD4+ absolut ≤200 sel/ μL. Variabel yang memenuhi syarat akan disertakan pada analisis multivariat dengan regresi logistik.Hasil. Dari 606 pasien HIV/AIDS dewasa yang dirawat inap (median usia 32 tahun; laki-laki 64,2%), sebanyak 122 (20,1%) baru terdiagnosis HIV selama rawat dan 251 (41,5%) dalam terapi ARV. Median lama rawat adalah 11 (rentang 2 sampai 75) hari. Sebanyak 425 (70,1%) pasien dirawat karena infeksi oportunistik. Mortalitas selama perawatan sebesar 23,4% dengan mayoritas penyebabnya (92,3%) terkait AIDS. Prediktor independen mortalitas yang bermakna adalah stadium klinis WHO 4 (OR=6,440; IK 95% 3,701-11,203), kadar hemoglobin <10 g/dL (OR=1,542; IK 95% 1,015-2,343) dan laju filtrasi glomerulus (LFG) estimasi <60 mL/min/1,73 m2 (OR=3,414; IK 95% 1,821-6,402).Simpulan. Proporsi mortalitas selama perawatan sebesar 23,4%. Stadium klinis WHO 4, kadar hemoglobin <10 g/dL dan kadar eLFG <60 mL/min/1,73 m2 merupakan prediktor independen mortalitas pasien HIV/AIDS dewasa saat rawat inap.Kata kunci: HIV/AIDS, prediktor mortalitas Predictors of Mortality in Hospitalized HIV/AIDS PatientsIntroduction. Human Immunodeficiency Virus/Acquired Immune Deficiency Syndrome (HIV/AIDS) is a big problem that threatening in Indonesia and many countries in the world. The knowledge on the characteristics and prediction of outcome were important for patients management. There are no studies on the predictors of mortality in Indonesia. Methods. We performed a retrospective cohort study among hospitalized patients with HIV/AIDS in Cipto Mangunkusumo Hospital between 2011-2013. Datas on clinical, laboratory measurement, outcome (mortality) and causes of death during hospitalization were gathered from medical records. Bivariate analysis using Chi- Square test were used to evaluate seven prognostic factors (male sex, not came from referral hospital, never received/failed to continue antiretroviral therapy (ART), clinical WHO stage 4, hemoglobin level <10 g/dL, eGFR level <60 mL/min/1.73 m2 and CD4+ count ≤200 cell/μL). Multivariate logistic regression analysis was performed to identify independent predictors of mortality. Results. Among 606 hospitalized HIV/AIDS patients (median age 32 years; 64.2% males), 122 (20.1%) were newly diagnosed with HIV infection during the hospitalization and 251 (41.5%) had previously received ART. Median length of stay was 11 (range 2 to 75) days. There were 425 (70.1%) patients being hospitalized due to opportunistic infection. In-hospital mortality rate was 23.4% with majority (92.3%) due to AIDS related illnesses. The independent predictors of mortality in multivariate analysis were clinical WHO stage 4 (OR=6.440; 95% CI 3.701-11.203), hemoglobin level <10 g/dL (OR=1.542; 95% CI 1.015- 2.343) and eGFR level <60 mL/min/1.73 m2 (OR=3.414; 95% CI 1.821-6.402). Conclusions. In-hospital mortality rate was 23.4%. Clinical WHO stage 4, hemoglobin level <10 g/dL and eGFR level <60 mL/ min/1.73 m2 were the independent predictors of in-hospital mortality among hospitalized patients with HIV/AIDS. Keywords: HIV/AIDS, predictors of mortality

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Proper levels of c-Myb are discretely defined at distinct steps of hematopoietic cell development

Blood ◽

10.1182/blood-2005-09-3846 ◽

2006 ◽

Vol 108 (3) ◽

pp. 896-903 ◽

Cited By ~ 51

Author(s):

Hiroshi Sakamoto ◽

Guoyou Dai ◽

Kaori Tsujino ◽

Kazuaki Hashimoto ◽

Xin Huang ◽

...

Keyword(s):

Endothelial Cells ◽

Progenitor Cells ◽

B Lymphocyte ◽

Embryonic Stem ◽

Cell Lineage ◽

Cell Development ◽

Hematopoietic Cell ◽

Hematopoietic Progenitor Cells ◽

Hematopoietic Progenitor ◽

Hematopoietic Cell Lineage

Abstract The definitive hematopoietic cell lineages have been proposed to originate from hemogenic endothelial cells during mouse embryogenesis. c-Myb is a transcription factor that is essential for the development of definitive hematopoiesis. To investigate the functional role of c-Myb in hematopoietic cell development from endothelial cells, we introduced a c-myb transgene expressed under the control of a tetracycline-regulated promoter into the c-myb–/– embryonic stem (ES) cell line, with the aim of inducing c-Myb expression at any stage and at any level. Induction of c-Myb expression after replating c-myb–/– endothelial cells rescued the generation and proliferation of definitive hematopoietic progenitor cells, suggesting that c-Myb expression in developing endothelial cells is not a prerequisite for their hematogenic potential. Overexpression of c-Myb, however, prevented the terminal differentiation of erythrocytes and megakaryocytes and completely abolished B-lymphocyte development. Our results indicate that c-Myb is a major factor that controls differentiation as well as proliferation of hematopoietic progenitor cells derived from hemogenic endothelial cells, and that appropriate levels of c-Myb protein are strictly defined at distinct differentiation steps of each hematopoietic cell lineage.

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The proportion of mitoses in different cell lineages changes during short-term culture of normal human bone marrow

Blood ◽

10.1182/blood.v67.5.1240.1240 ◽

1986 ◽

Vol 67 (5) ◽

pp. 1240-1243 ◽

Cited By ~ 10

Author(s):

M Keinanen ◽

S Knuutila ◽

CD Bloomfield ◽

E Elonen ◽

A de la Chapelle

Keyword(s):

Bone Marrow ◽

Cell Lineage ◽

Great Majority ◽

Hematopoietic Cell ◽

Human Bone ◽

Human Bone Marrow ◽

Immunofluorescent Staining ◽

Cytoplasmic Staining ◽

Cell Lineages ◽

Mitotic Cells

Abstract To determine the hematopoietic cell lineage of mitotic cells in human bone marrow on direct examination and after 24-hour culture, marrow mitoses from four healthy individuals were studied, using a new technique that allows analysis of karyotypes in cells whose cell membrane and cytoplasm have been preserved. Mitoses were identified as being of erythroid lineage by immunofluorescent staining for surface glycophorin A and as being of granulocytic lineage by cytoplasmic staining for Sudan black B. On direct marrow examination without prior culture, the great majority of mitoses (74% to 90%) were of erythroid lineage; only a few (0% to 10%) were granulocytic. After 24-hour culture, the percentage of erythroid mitoses (15% to 40%) decreased, while the percentage of granulocytic mitoses (58% to 87%) increased strikingly. These data indicate that mitotic cells of different hematopoietic cell lineages predominate in marrow at different culture times and offer a plausible explanation for the high frequency of normal karyotypes in acute myeloid leukemia after direct marrow cytogenetic evaluation.

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