scholarly journals Electrokinetic, oxidative and aggregation properties of red blood cells in the postoperative period following kidney transplantation

2020 ◽  
Vol 22 (2) ◽  
pp. 72-79
Author(s):  
A. V. Deryugina ◽  
O. P. Abaeva ◽  
S. V. Romanov ◽  
M. V. Vedunova ◽  
E. N. Ryabova ◽  
...  
Keyword(s):  
Kidney Transplantation ◽  
Red Blood Cells ◽  
Electrophoretic Mobility ◽  
Postoperative Period ◽  
Blood Cells ◽  
Gradual Increase ◽  
Cellular Level ◽  
Stress Reaction ◽  
Systemic Level ◽  
Peroxidation Processes

Objective: to study the electrokinetic and aggregation properties, as well as the pro-oxidant and antioxidant processes in red blood cells following kidney transplantation in donors and in recipients in the postoperative period. Materials and methods. Blood from 12 recipients and 5 kidney donors over time – before transplantation, as well as at week 1, months 1, 2, 7, 10 and 12 after surgery, as well as from 8 healthy volunteers who formed the control group. We used microelectrophoresis to measure the electrophoretic mobility of red blood cells, characterizing the electrokinetic properties of cells. Aggregation was calculated microscopically by counting unaggregated red blood cells. Malondialdehyde concentration was measured spectrophotometrically at its absorbance maximum at 530 nm by reaction with thiobarbituric acid. Catalase activity was analyzed by reducing hydrogen peroxide in the sample spectrophotometrically at 240 nm wavelength. The obtained values were compared using the Mann–Whitney U test. Results. Decreased electrophoretic mobility of red blood cells within 2 months after transplantation was associated with increased malondialdehyde concentration and erythrocyte aggregation, decreased catalase activity in kidney recipients, followed by restoration of indicators to the control values. Electrophoretic mobility of red blood cells decreased, while malondialdehyde concentrations increased in donors after surgery. However, the increase was less pronounced than in recipients. The changes indicate that the postoperative period causes changes at the cellular level both in donors and in recipients. This is manifested by decreased stability of erythrocyte membrane structure, which is largely determined by lipid peroxidation processes. At the systemic level, a change in the electrophoretic mobility of red blood cells indicates a stress reaction before and after kidney transplantation in recipients within 2 months after surgery, and in donors in 1–2 months in the postoperative period with gradual increase in the body’s resistance. Conclusion. Kidney transplantation is manifested at the cellular and systemic levels. At the cellular level, there is decreased stability of the membrane structure, which is largely determined by lipid peroxidation processes. At the systemic level, a change in the electrophoretic mobility of red blood cells indicates a stress reaction with gradual increase in the body’s resistance. The data obtained demonstrate changes in the functional properties of red blood cells both in kidney transplant recipients and in donors. These changes need to be taken into account when carrying out therapeutic measures.

DIAGNOSTIC CAPABILITIES OF THE ELECTROPHORETIC MOBILITY OF RED BLOOD CELLS AND BUCCAL CELLS IN STRESS

2019 ◽  
pp. 106-111
Author(s):  
А.В. Дерюгина ◽  
М.Н. Иващенко ◽  
П.С. Игнатьев ◽  
А.Г. Самоделкин ◽  
М.В. Золотова ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Red Blood Cells ◽  
Electrophoretic Mobility ◽  
Blood Cells ◽  
Information Technologies ◽  
Stress Reaction ◽  
Medical Laboratory ◽  
Sharp Change ◽  
Epinephrine Injection ◽  
Acting Force

Диагностика стресс-реактивности по анализу гормонального состояния организма, является сложной, экономически затратной и трудоемкой процедурой исследования. В связи с этим актуален поиск информационных технологий, которые достаточно легко реализуются в любой медицинской лаборатории и позволяют оценить стресс-реакцию организма. Цель работы - оценка диагностических возможностей электрофоретической подвижности эритроцитов и клеток буккального эпителия в процессе развития стрессовой реакции. Методика. Для моделирования стресса использовали внутрибрюшинное введение крысам раствора адреналина-гидрохлорида (0,1 мг/кг) однократно или ежедневно в течение 3 сут эксперимента соответственно. Контролем служили крысы, получавшие физиологический раствор (в/б) в том же объеме. Буккальный эпителий исследовали на 7-е сут после введения адреналина. Мазки клеток эпителия окрашивали по Романовскому-Гимза, на препаратах подсчитывали количество клеток с микроядрами на 1000 клеток. Электрофоретическую подвижность эритроцитов оценивали методом микроэлектрофореза. Результаты. Наблюдалась качественно однотипная динамика показателя электрофоретической подвижности эритроцитов, количественно зависящая от силы воздействия. При слабых стимулах скорость изменений электрофоретической подвижности эритроцитов незначительна и сочетается с развитием адаптивных процессов, связанных с репарацией генетических повреждений. Увеличение степени стрессового воздействия путем трехкратного введения адреналина приводит к резкому изменению электрофоретической подвижности эритроцитов и росту числа эпителиоцитов с различными патологическими изменениями. Заключение. Показана возможность использования данного метода в качестве маркерной характеристики интенсивности стрессовой реакции с выявлением ее генотоксичности. Diagnostics of stress reaction using an analysis of hormonal state is a difficult, costly, and labor-consuming research. Therefore, searching for information technologies, which could be easily used in any medical laboratory and would allow evaluating the stress reaction is relevant. We studied the electrophoretic mobility of red blood cells (RBC) and buccal epithelial cells of rats exposed to stress induced by a single or repeated intraperitoneal epinephrine injection. This study showed that these methods could be used as the marker characteristics of stress reaction intensity and genotoxicity. Qualitative changes in the electrophoretic mobility of RBC were similar but quantitative characteristics depended on the acting force. Under a weak stimulus, changes in the electrophoretic mobility of RBC were slight and associated with development of adaptive processes related with repair of genetic damage. A further increase in the acting force led to a sharp change in the electrophoretic mobility of RBC and an increase in the number of epithelial cells with pathological alterations.

To hematology of scurvy

1904 ◽  
Vol 4 (1-2) ◽  
pp. 104-105
Author(s):  
M Beloglazov
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Gradual Increase ◽  
Absolute Amount ◽  
The Absolute

11 people of scurvy patients of various ages (from 14 to 52 years old) were investigated by the author from the side of blood changes. The main results of the study are as follows: i) The absolute amount of hemoglobin in all the cases studied is reduced; the gradual increase during recovery does not reach the norm even when the number of red blood cells becomes normal.

scholarly journals Electrophoretic Mobility of Red Blood Cells in Patients with Severe Forms of Intoxication

2007 ◽  
Vol 3 (6) ◽  
pp. 75
Author(s):  
I. Ya. Golovetsky ◽  
V. V. Moroz ◽  
L. S. Biryukova ◽  
G. I. Kozinets ◽  
O. V. Popova
Keyword(s):  
Red Blood Cells ◽  
Electrophoretic Mobility ◽  
Blood Cells

scholarly journals ELECTROPHORETIC MOBILITY AND AGGLUTINABILITY OF RED BLOOD CELLS: A "NEW" POLYMORPHISM IN MICE

1974 ◽  
Vol 139 (2) ◽  
pp. 313-322 ◽  
Author(s):  
Pablo Rubinstein ◽  
Ngukoy Liu ◽  
Edwin W. Streun ◽  
Francine Decary
Keyword(s):  
Red Blood Cells ◽  
Genetic Control ◽  
Electrophoretic Mobility ◽  
Blood Cells ◽  
Quantitative Method ◽  
Coat Color ◽  
Inbred Strains ◽  
Red Cells ◽  
Inbred Strains Of Mice

A quantitative method has been developed to determine agglutinability of mouse red blood cells. Tests with different inbred strains of mice revealed only two phenotypes. The same inbred strains were tested with the cytopherometer to determine the electrophoretic mobility of the corresponding red cells. Again, two phenotypes were uncovered, and faster mobility was found in the red cells that had higher agglutinability. The genetic control of this character is autosomal and codominant, and segregates independently of H-2 and coat color.

Actions of thiocyanate and N-phenylmaleimide on volume-responsive Na and K transport in dog red cells

1992 ◽  
Vol 262 (2) ◽  
pp. C418-C421 ◽  
Author(s):  
J. C. Parker ◽  
G. C. Colclasure
Keyword(s):  
Red Blood Cells ◽  
Cell Volume ◽  
Electrophoretic Mobility ◽  
Blood Cells ◽  
Membrane Transporters ◽  
Red Cells ◽  
Transport Systems ◽  
Volume Changes ◽  
Sulfhydryl Reagent ◽  
K Transport

Two sets of observations suggest a linkage between volume-responsive Na and K transport systems in dog red blood cells. 1) The lyotropic anion thiocyanate inhibits shrinkage-induced Na-H exchange and stimulates swelling-induced K-Cl cotransport. 2) The effect of a brief incubation with N-phenylmaleimide (NPM) on Na and K transport depends on the volume of the cells at the time of exposure to the sulfhydryl reagent. Cells shrunken during the NPM incubation and then brought back to normal volume behave as though they were still shrunken, i.e., they show an increased Na flux and a decreased K flux. Cells incubated with NPM in a swollen state retain fluxes characteristic of swollen cells when returned to a normal volume. The electrophoretic mobility of the membrane-associated enzyme glyceraldehyde-3-phosphate dehydrogenase is influenced by the cell volume at the time of NPM exposure. These findings point to the existence of a system in cells that perceives volume changes and coordinates the responses of membrane transporters.

Potential immunohaematological effects of persistent organic pollutants on chinstrap penguin

2015 ◽  
Vol 27 (4) ◽  
pp. 373-381 ◽  
Author(s):  
S. Jara-Carrasco ◽  
M. González ◽  
D. González-Acuña ◽  
G. Chiang ◽  
J. Celis ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Organic Pollutants ◽  
Persistent Organic Pollutants ◽  
Blood Cells ◽  
Cellular Level ◽  
Chinstrap Penguin ◽  
Pygoscelis Antarctica ◽  
Cell Alterations ◽  
Cytological Alterations ◽  
The Impact

AbstractIt has been demonstrated that persistent organic pollutants (POPs) can affect the immune system of mammals and birds. In this study, the concentration of different POPs and leukocytes in blood samples from three chinstrap penguin (Pygoscelis antarctica) populations was analysed in order to assess the impact on haematological parameters. Using blood sample smears, basophils, eosinophils, heterophils, lymphocytes and monocytes were quantified. Mature and immature red blood cells were counted and cell alterations in both white and red blood cells were analysed. At the same time, whole blood was analysed for POPs. The results showed that contaminants, such as dichlorodiphenyltrichloroethane and its metabolites (ΣDDT), as well as polychlorinated biphenyls (ΣPCB), had significant correlations to eosinophils, lymphocytes and heterophils. This indicates possible immunohaematological alterations derived from exposure to such contaminants. Cytological alterations were also observed, such as cytotoxic granules, toxic heterophils, and atypical and granulated lymphocytes, which would demonstrate that these seabirds are being exposed to stress agents that could be producing some alterations at a leukocytary cellular level.

scholarly journals ANTIBODY SYNTHESIS AT THE CELLULAR LEVEL

1966 ◽  
Vol 124 (5) ◽  
pp. 953-969 ◽  
Author(s):  
Hans Wigzell
Keyword(s):  
Red Blood Cells ◽  
Antibody Production ◽  
Blood Cells ◽  
Inhibitory Effect ◽  
Cellular Level ◽  
Antibody Synthesis ◽  
Feedback Factor ◽  
Specific Suppression ◽  
Direct Suppression

The specific suppressing activity of passively administered antibody on 7S antibody synthesis against sheep and chicken red blood cells has been investigated at the cellular level using the indirect hemolytic agar-plaque technique. 7S antibody production was found to be sensitive to antibody-induced suppression. No inhibitory effect of transferred antibody was seen until 48 to 72 hr after administration. This indicates that the action of antibody is not by direct suppression of synthesis of already committed cells but rather by removal from the system of the stimulus for maintenance of 7S synthesis. The sensitivity of the 7S system to inhibition decreases with time after immunization but significant specific suppression could still be obtained if transfer of antibody was delayed until 40 days after immunization. The present findings emphasize the role of antibody as a feedback factor during a substantial postpeak period of 7S antibody synthesis and suggest an important role of antigen in stabilizing the 7S antibody production.

Sign in / Sign up

Export Citation Format

Share Document