To hematology of scurvy

1904 ◽  
Vol 4 (1-2) ◽  
pp. 104-105
Author(s):  
M Beloglazov
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Gradual Increase ◽  
Absolute Amount ◽  
The Absolute

11 people of scurvy patients of various ages (from 14 to 52 years old) were investigated by the author from the side of blood changes. The main results of the study are as follows: i) The absolute amount of hemoglobin in all the cases studied is reduced; the gradual increase during recovery does not reach the norm even when the number of red blood cells becomes normal.

scholarly journals RED CELL AND PLASMA RADIOACTIVE COPPER IN NORMAL AND ANEMIC DOGS

1942 ◽  
Vol 75 (5) ◽  
pp. 489-494 ◽  
Author(s):  
H. Yoshikawa ◽  
P. F. Hahn ◽  
W. F. Bale
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Gradual Increase ◽  
Red Cells ◽  
Red Cell ◽  
Hematopoietic Activity

Following ingestion of radioactive copper by the dog, the metal appears quickly in the plasma, the concentration reaching its peak in from 2 to 5 hours, after which it falls abruptly. Demonstrable amounts are still present after 2 days. The radioactive copper likewise appears rapidly in the circulating red blood cells, and there is a slow but gradual increase in its concentration over a 2 day period. It is suggested by the data that in the animals in which hematopoietic activity is accelerated the uptake of radio-copper is somewhat more extensive. The possibility that this is due to greater adsorption by the new red cells in these instances is not ruled out.

scholarly journals Association of general blood count indicators with the severity of COVID-19 in hospitalized patients

2021 ◽  
Vol 2 (1) ◽  
pp. 90-101
Author(s):  
N. S. Gubenko ◽  
A. A. Budko ◽  
A. G. Plisyuk ◽  
I. A. Orlova
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Clinical Condition ◽  
Blood Test ◽  
Medical Center ◽  
Absolute Number ◽  
Hospitalized Patients ◽  
Lung Damage ◽  
The Absolute ◽  
Severity Of The Disease

Purpose: to study the relationship of the indicators of the general blood test with the severity of the course of COVID-19 in hospitalized patients.Materials and methods: the study included 165 patients (92 men — 55.8%, and 73 women — 44.2%, the average age — 59.9 years) who were treated at the Moscow State University Medical Center in the period from April to June 2020 with a diagnosis of COVID-19. All patients underwent: general blood test, CRP, CT of the lungs. The severity of the clinical condition was assessed using the SHOCK-COVID and NEWS-2 scales.Results: a more severe clinical condition of patients and a greater severity of lung damage on admission were statistically significantly associated with a decrease in the number of red blood cells and hemoglobin, as well as with a greater width of the distribution of red blood cells (RDW-SD). The rate of erythrocyte sedimentation (ESR) was significantly associated with the clinical condition of patients evaluated by SHOCK-COVID (r=0.61, p<0.001) and the marker of CRP inflammation (r=0.55, p<0.001). An increase in the absolute number of neutrophils (N), a decrease in the absolute number of lymphocytes (L), and, as a result, an increase in the N/L ratio index was a marker of a more severe course of the disease. It was the N/L index that had the maximum correlation coefficient with the most commonly used marker of systemic inflammation - CRP (r=0.50, p<0.001). The decrease in the level of CRP by discharge was associated with a significant decrease in ESR (r=0.36, p<0.001), the index of the ratio of neutrophil and lymphocyte levels (N/L) (r=0.39, p<0.001), and an increase in the width of the distribution of red blood cells (RDW-SD r=0.25, p<0.01; RDW-CV r=0.57, p<0.001).Conclusions: the most informative indicators of the general blood test at admission to the hospital, allowing to assess the severity of the disease — the width of the distribution of red blood cells, the index of the ratio of neutrophils to lymphocytes and ESR.

Junction Thrombi Deposited on Conducting Materials at Different Potentials

1970 ◽  
Vol 24 (01/02) ◽  
pp. 273-285 ◽  
Author(s):  
M Ranganathan ◽  
J. G Stempak ◽  
S Srinivasan ◽  
P. N Sawyer
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Gradual Increase ◽  
Conducting Materials ◽  
Electric Potentials ◽  
Blood Elements ◽  
Surface Changes

SummaryThe present study reveals that the deposits on metal prostheses maintained at positive (spontaneous) or negative electric potentials are in histologic composition and characteristics of the nature of a thrombus and not of a clot in vitro. The study also shows that thrombic deposits on copper prostheses maintained at negativ potentials are broadly similar in nature and composition though there are some significant distinctions among them. In the study on the sample from a copper prosthesis maintained at a potential of —160 mv where only junctional thrombi were formed, it is observed that within about 200 μl from the prosthetic surface the pattern of composition of thrombus changes. The predominant platelet population with very few erythrocytes and fibrin near the prosthetic surface changes with gradual increase in number of red blood cells into a region predominantly composed of red blood cells. Several distorted blood elements with electron dense inclusions in them, observed close to the prosthetic surface, were more frequently observed in the sample from the copper prosthesis at a potential of —160 mv than in the sample at —110 mv.

scholarly journals Electrokinetic, oxidative and aggregation properties of red blood cells in the postoperative period following kidney transplantation

2020 ◽  
Vol 22 (2) ◽  
pp. 72-79
Author(s):  
A. V. Deryugina ◽  
O. P. Abaeva ◽  
S. V. Romanov ◽  
M. V. Vedunova ◽  
E. N. Ryabova ◽  
...  
Keyword(s):  
Kidney Transplantation ◽  
Red Blood Cells ◽  
Electrophoretic Mobility ◽  
Postoperative Period ◽  
Blood Cells ◽  
Gradual Increase ◽  
Cellular Level ◽  
Stress Reaction ◽  
Systemic Level ◽  
Peroxidation Processes

Objective: to study the electrokinetic and aggregation properties, as well as the pro-oxidant and antioxidant processes in red blood cells following kidney transplantation in donors and in recipients in the postoperative period. Materials and methods. Blood from 12 recipients and 5 kidney donors over time – before transplantation, as well as at week 1, months 1, 2, 7, 10 and 12 after surgery, as well as from 8 healthy volunteers who formed the control group. We used microelectrophoresis to measure the electrophoretic mobility of red blood cells, characterizing the electrokinetic properties of cells. Aggregation was calculated microscopically by counting unaggregated red blood cells. Malondialdehyde concentration was measured spectrophotometrically at its absorbance maximum at 530 nm by reaction with thiobarbituric acid. Catalase activity was analyzed by reducing hydrogen peroxide in the sample spectrophotometrically at 240 nm wavelength. The obtained values were compared using the Mann–Whitney U test. Results. Decreased electrophoretic mobility of red blood cells within 2 months after transplantation was associated with increased malondialdehyde concentration and erythrocyte aggregation, decreased catalase activity in kidney recipients, followed by restoration of indicators to the control values. Electrophoretic mobility of red blood cells decreased, while malondialdehyde concentrations increased in donors after surgery. However, the increase was less pronounced than in recipients. The changes indicate that the postoperative period causes changes at the cellular level both in donors and in recipients. This is manifested by decreased stability of erythrocyte membrane structure, which is largely determined by lipid peroxidation processes. At the systemic level, a change in the electrophoretic mobility of red blood cells indicates a stress reaction before and after kidney transplantation in recipients within 2 months after surgery, and in donors in 1–2 months in the postoperative period with gradual increase in the body’s resistance. Conclusion. Kidney transplantation is manifested at the cellular and systemic levels. At the cellular level, there is decreased stability of the membrane structure, which is largely determined by lipid peroxidation processes. At the systemic level, a change in the electrophoretic mobility of red blood cells indicates a stress reaction with gradual increase in the body’s resistance. The data obtained demonstrate changes in the functional properties of red blood cells both in kidney transplant recipients and in donors. These changes need to be taken into account when carrying out therapeutic measures.

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

1973 ◽  
Vol 31 ◽  
pp. 328-329
Author(s):  
John A. Trotter
Keyword(s):  
Red Blood Cells ◽  
Analytical Method ◽  
Blood Cells ◽  
Guinea Pigs ◽  
Specific Protein ◽  
Visual Examination ◽  
Hemoglobin Synthesis ◽  
Peroxidatic Activity ◽  
Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

Scanning electron microscopy of erythrophagocytosis by Entamoeba histolytica trophozoites

1992 ◽  
Vol 50 (1) ◽  
pp. 880-881
Author(s):  
Victor Tsutsumi ◽  
Adolfo Martinez-Palomo ◽  
Kyuichi Tanikawa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Red Blood Cells ◽  
Entamoeba Histolytica ◽  
Causative Agent ◽  
Blood Cells ◽  
Protozoan Parasite ◽  
Complex Phenomenon ◽  
Great Capacity ◽  
Scanning Electron

The protozoan parasite Entamoeba histolytica is the causative agent of amebiasis in man. The trophozoite or motile form is a highly dynamic and pleomorphic cell with a great capacity to destroy tissues. Moreover, the parasite has the singular ability to phagocytize a variety of different live or death cells. Phagocytosis of red blood cells by E. histolytica trophozoites is a complex phenomenon related with amebic pathogenicity and nutrition.

Ultrastructural observations on the in vitro cytoadherence of Plasmodium falciparum infected red blood cells

1990 ◽  
Vol 48 (3) ◽  
pp. 914-915
Author(s):  
D.J.P. Ferguson ◽  
A.R. Berendt ◽  
J. Tansey ◽  
K. Marsh ◽  
C.I. Newbold
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Umbilical Vein ◽  
Cell Types ◽  
Amelanotic Melanoma ◽  
Cytoplasmic Process ◽  
Umbilical Vein Endothelial Cells

In human malaria, the most serious clinical manifestation is cerebral malaria (CM) due to infection with Plasmodium falciparum. The pathology of CM is thought to relate to the fact that red blood cells containing mature forms of the parasite (PRBC) cytoadhere or sequester to post capillary venules of various tissues including the brain. This in vivo phenomenon has been studied in vitro by examining the cytoadherence of PRBCs to various cell types and purified proteins. To date, three Ijiost receptor molecules have been identified; CD36, ICAM-1 and thrombospondin. The specific changes in the PRBC membrane which mediate cytoadherence are less well understood, but they include the sub-membranous deposition of electron-dense material resulting in surface deformations called knobs. Knobs were thought to be essential for cytoadherence, lput recent work has shown that certain knob-negative (K-) lines can cytoadhere. In the present study, we have used electron microscopy to re-examine the interactions between K+ PRBCs and both C32 amelanotic melanoma cells and human umbilical vein endothelial cells (HUVEC).We confirm previous data demonstrating that C32 cells possess numerous microvilli which adhere to the PRBC, mainly via the knobs (Fig. 1). In contrast, the HUVEC were relatively smooth and the PRBCs appeared partially flattened onto the cell surface (Fig. 2). Furthermore, many of the PRBCs exhibited an invagination of the limiting membrane in the attachment zone, often containing a cytoplasmic process from the endothelial cell (Fig. 2).

Phagocytosis of opsonized red blood cells by hepatocytes after gene transfer of chimeric Fc γR III Aα and γcDNA

2001 ◽  
Vol 120 (5) ◽  
pp. A356-A357
Author(s):  
M FURUKAWA ◽  
Y MAGAMI ◽  
D NAKAYAMA ◽  
F MORIYASU ◽  
J PARK ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Red Blood Cells ◽  
Blood Cells
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