Haematology values and effect of age and reproductive stage on haematological parameters of Chios sheep

The main aim of the present study was to determine the values of the haematological parameters of dairy sheep reared under intensive and semi-intensive conditions, as well as to test for the effects of age and reproductive stage of the animals on the values of these parameters. In total, 200 clinically healthy Chios sheep from 10 farms of Central Macedonia were used in the study. For the determination of the effect of age 150 sheep were assigned in three groups. Group A consisted of 50 lambs aged 3-6 months (meantSD: 4.45±1.22 months), group Β of 50 non-pregnant ewes into lactation aged 1-3 years (meantSD: 2.18±0.74 years) and group C of 50 non-pregnant ewes into lactation aged more than 3 years (meantSD: 5.88±1.87 years). For evaluating the effect of reproductive status 50 pregnant ewes in dry period were used, 15-30 days before the expected day of lambing (group D), along with the 100 non-pregnant ewes into lactation of groups Β and C (group E). Blood sampling was performed once, in dry ewes from December to January, and in lambs and lactating ewes from March to May. The mean values of the determined haematological parameters were within the range provided by the literature, except for red blood cells count of ewes. The results also showed that haematocrit, haemoglobin concentration and red blood cells, white blood cells, neutrophils, lymphocytes, eosinophils and platelets count were significantly affected by the age; only monocytes and eosinophils count by the reproductive stage of the animals.

Download Full-text

HAEMATOLOGICAL PARAMETERS AMONG SUDANESE PATIENTS WITH CHRONIC RENAL FAILURE

International Journal of Research -GRANTHAALAYAH ◽

10.29121/granthaalayah.v4.i1.2016.2842 ◽

2016 ◽

Vol 4 (1) ◽

pp. 50-54

Author(s):

RaianBakhet Yassein ◽

Nada Omer Alseedig ◽

SihamKhalifaAbd Allah ◽

AreegAlsail Mohmmed ◽

NosibaAbdelmajid Alballah ◽

...

Keyword(s):

Renal Failure ◽

Platelet Count ◽

Chronic Renal Failure ◽

Red Blood Cells ◽

Blood Cells ◽

White Blood Cells ◽

Haemoglobin Concentration ◽

Haematological Parameters ◽

Common Causes ◽

Hb Concentration

Chronic renal failure is one of the more common causes of morbidity and mortality among Sudanese. This study describes altered haemoglobin concentration (Hb), haematochrit percent (Hct), red blood cells count (RBCs), platelet count (Plts) and white blood cells count (WBCs) in patients with chronic renal failure and describes the effect of disease duration on haemoglobin concentration and red blood cells count. Methods: Haematological parameters in (50) patients with Chronic renal failure and (50) age and sex matched controls were evaluated, living in Shendi locality in northern Sudan. The results of this study showed that mean of Hb concentration, RBCs count, Hct percent and platelet count were exhibited significant decreased, but mean of WBCs count was insignificantly increased. Increased duration of the disease lead to significantly decreased on mean of Hb concentration and RBCs count. Chronic renal failure is associated with different degrees of reduced haematological parameters. Also increase duration of the disease lead to significantly decrease in mean of Hb concentration and RBCs count.

Download Full-text

Reference intervals for haematological parameters in the Lusitano horse breed

Acta Veterinaria Hungarica ◽

10.1556/004.2018.047 ◽

2018 ◽

Vol 66 (4) ◽

pp. 530-541 ◽

Cited By ~ 1

Author(s):

Ana Cristina Silvestre-Ferreira ◽

Mário Cotovio ◽

Mário Maia ◽

Felisbina Queiroga ◽

M João Pires ◽

...

Keyword(s):

Blood Cells ◽

White Blood Cells ◽

Age Groups ◽

Haemoglobin Concentration ◽

Reference Intervals ◽

Haematological Parameters ◽

Distribution Width ◽

Mean Values ◽

Corpuscular Haemoglobin ◽

Mean Corpuscular Haemoglobin

The Lusitano horse is an autochthonous Portuguese breed with a growing worldwide expansion. Our objective was to establish reference intervals for haematological parameters using the haematological cell counter LaserCyte (IDEXX). For this purpose, blood samples from 100 healthy adult horses (13 females and 87 males, ranging from 3 to 25 years of age) were analysed. The reference intervals were estimated following the ASVCP guidelines with the Reference Value Advisor software. The obtained reference intervals were 6.4–10.1 × 1012/L for red blood cells, 30.6–45.1% for haematocrit, 11.6–17.1 g/dL for haemoglobin, 42.8–53.2 fL for mean corpuscular volume (MCV), 15.5–20.8 pg for mean corpuscular haemoglobin (MCH), 33.7–39.4 g/dL for mean corpuscular haemoglobin concentration, 17.8–20.3% for red cell distribution width (RDW), 4.5–10.1 × 109/L for white blood cells, 2.2–6.0 × 109/L for neutrophils, 0.9–4.9 × 109/L for lymphocytes, 0.2–0.5 × 109/L for monocytes, 0.1–0.6 × 109/L for eosinophils, 0.0–0.1 × 109/L for basophils, 78.5–172.2 K/mL for platelets, 4.3–9.4 fL for mean platelet volume, 18.8–24.2% for platelet distribution width, and 0.06–0.12% for plateletcrit. LaserCyte equine reference intervals are transferable to the Lusitano horse for 18 of the 22 analytes studied. Regarding age, significant statistical differences were observed for MCV, RDW, neutrophils and lymphocytes between the mean values of young (3–6 years old), middle-aged (7–14 years old) and old (< 15 years old) age groups. MCH means were statistically significantly different between the three age groups. The haematological reference intervals established in this study might represent a valuable and applicable tool for haematological assessment of adult Lusitano horses, providing useful information that helps clinicians to interpret clinical data.

Download Full-text

Comparative assessment of Chemosuppressive activity of three Chinese teas on Plasmodium berghei infected mice

The Journal of Phytopharmacology ◽

10.31254/phyto.2018.7405 ◽

2018 ◽

Vol 7 (4) ◽

pp. 376-383

Author(s):

Bankole Olukayode Olusola ◽

◽

Oderinde Abdulganiyu Olumuyiwa ◽

Keyword(s):

Body Weight ◽

Red Blood Cells ◽

Plasmodium Berghei ◽

Blood Cells ◽

Haemoglobin Concentration ◽

Treated Group ◽

Antimalarial Drugs ◽

Comparative Assessment ◽

Haematological Parameters ◽

Weight Changes

Malaria, a hazardous infirmity caused by a parasitic malady of the red blood cells, is without question harming to the wellbeing. In the present investigation, the chemosuppresive and haematopoietic activities of 200 mg/kg and 400 mg/kg body weight of unrefined ethanolic concentrates of three Chinese green teas (BIA 849, TD 570 and GB/T19598) were assessed using the 4-day suppressive anti-plasmodial assay in mice Plasmodium berghei (NK65 strain) pre-infected mice. The effect of the extracts on weight of the animals was evaluated. It was observed that 200 mg/kg bw (body weight) of BIA 849 and GB/T19598 were as potent as 5 mg/kg bw of chloroquine, with percentage suppressions of 58.97 ± 5.04, 57.63 ± 5.62 and 57.50 ± 4.5, respectively. TD570 at 200 mg/kg bw was more effective in suppressing plasmodium. 400 mg/kg body weight of TD570 and GB/T19598 extracts were more potent than 5 mg/kg bw of chloroquine having 100 % chemosuppression. The chemosuppression of BIA 849 did not change altogether at 400 mg/kg bw. The haematological parameters, WBC, RBC and MCV did not significantly change in the groups treated with the tea extracts utilizing suppressive model of malaria treatment contrasted with the uninfected group and were comparable to those treated with chloroquine. Haemoglobin concentration nonetheless, varied significantly with respect to the uninfected group. Weight changes were most significant with 200 mg/kg bw of TD 570 treated group (32 % increase) on suppression. All in all, the green teas displayed high chemosuppressive and haematopoietic possibilities and are thusly prescribed as contender for additionally screening as elective antimalarial drugs

Download Full-text

Haematological Changes in Donors Post Apheresis

JOURNAL FOR CLINICAL AND DIAGNOSTIC RESEARCH ◽

10.7860/jcdr/2021/48896.15157 ◽

2021 ◽

Author(s):

. Nikhil ◽

Subhashish Das ◽

. Snigdha

Keyword(s):

Platelet Count ◽

Blood Cells ◽

Complete Blood Count ◽

White Blood Cells ◽

Haemoglobin Concentration ◽

Point Of View ◽

Haematological Parameters ◽

Cross Sectional ◽

Wbc Count ◽

Rbc Count

Introduction: The productivity, quality of platelet apheresis collection has improved because of the considerable advancement in the automated cell separators. Automated cell separators have lot of sizeable scientific advances, but the alertness has been centered to Platelet Concentrates (PCs) quality than on safety of donor. Aim: To find the changes in haematological parameters and the consequences of apheresis and plateletpheresis on donor’s health. Materials and Methods: It was observational cross-sectional study done in laboratory at RL Jalappa Blood Bank, Tamaka, Kolar, Karnataka, India. The study was done from March 2019 to August 2020. A total of 300 healthy donors (plateletpheresis donors) were involved in the study. The plateletpheresis (Haemonetics MCS), predonation and postdonation haematological parameters such as haemoglobin concentration, Haematocrit (Hct), platelet, white and red blood cell count were calculated in all donors. The samples for Complete Blood Count (CBC) were secured from the donors, at the beginning and end of the procedure. Postdonation haematological parameters such as platelet count, haemoglobin, haematocrit, White Blood Cells (WBC), Red Blood Cells (RBC) counts of the donor was inscribed and comparison was done with the pre donation haematological parameters. Quality control of all Single Donor Platelet (SDP) products was done. All donors were evaluated for adverse donor reactions. The mean pre and post plateletpheresis values comparison was done utilising paired t-test. Statistical analysis was accomplished utilising Statistical Package for the Social Sciences (SPSS) software version 16.0. Results: Platelet count, haemoglobin, WBC count, RBC count and haematocrit were jotted down from 262 donors and a significant decrease was noticed in these parameters postdonation. Donor parameter platelet count (lac/mL) value was decreased from 273.57-224.28 whereas WBC count (cu/mm) predonation value decreased from 9.91-8.86 Postdonation, haemoglobin (g/dL) value decreased from 14.46-12.91, haematocrit (%) decreased slightly from 45.19-44.19, RBC count (million/mm3) decreased from 5.21-5.01. This concluded that the values decreased postdonation. Conclusion: The study conducted was safe from donor’s point of view. SDP is very effective in treatment of thrombocytopenia and is safe from recipient’s point of view.

Download Full-text

STUDIES IN THE BLOOD CYTOLOGY OF THE RABBIT

Journal of Experimental Medicine ◽

10.1084/jem.53.5.695 ◽

1931 ◽

Vol 53 (5) ◽

pp. 695-714

Author(s):

Albert E. Casey

Keyword(s):

Red Blood Cells ◽

Significant Relationship ◽

Blood Cells ◽

Inverse Relationship ◽

White Blood Cells ◽

Cell Types ◽

Normal Male ◽

Hemoglobin Content ◽

Mean Values ◽

Observation Period

1. Statistical analyses have been made of the weekly variations in the blood counts of groups of normal rabbits to find whether there exists any relationship between the numerical changes occurring in the various cell types. Consecutive blood counts and differential white cell determinations on five groups of normal male rabbits comprising 45 animals in all were made at weekly intervals from October, 1927, to June, 1929, the number of observations on each group varying from eight to thirty-five. 2. The following relationships between the varying group means were found to be consistent and significant:—The number of the red blood cells varied with the amount of hemoglobin per cubic millimeter and with the number of lymphocytes. There was an inverse relationship between the amount of hemoglobin and the number of monocytes. The neutrophiles varied in number with the monocytes; the basophiles with the eosinophiles; and the eosinophiles with the monocytes. Other associations not always similar but of high significance as far as the combined values were concerned, were the relations of the red blood cells with the basophiles and the monocytes. The relations of the neutrophiles with the red blood cells and the hemoglobin were very irregular. 3. Significant association of the white blood cells with variations in the red blood cells and the hemoglobin content were observed. The numerical variations in the group means of the total white cells were associated with similar variations in the group means of the neutrophiles, the lymphocytes, the monocytes, the basophiles, and the eosinophiles almost to the degree of their numerical occurrence in the peripheral blood. 4. With the exception of the total white cells, approximately only half the variations in the group levels of the various cells and of the hemoglobin content can be accounted for on the basis of simultaneous associations with each other. 5. The red blood cells, the lymphocytes, and the basophiles as one group, the eosinophiles and the monocytes as another group, and the hemoglobin content and the neutrophiles as a third group, described a definite shift from a high to a low numerical value during the 2 year observation period. From the standpoint of the magnitude of the shift, the basophiles, the eosinophiles, the monocytes, the lymphocytes, and the red blood cells participated in the order mentioned. The neutrophiles were only slightly affected and the hemoglobin content relatively not at all. 6. No significant relationship was ever found, even in the component groups, between the weekly mean values of the following: the hemoglobin with the basophiles, the eosinophiles, or the lymphocytes; the neutrophiles with the basophiles or the eosinophiles; and the lymphocytes with the eosinophiles or the monocytes.

Download Full-text

Comparative effect of genotype and season on the haematological profile of two laying chicken strains in southwest Nigeria

Agricultura tropica et subtropica ◽

10.2478/ats-2020-0002 ◽

2020 ◽

Vol 53 (1) ◽

pp. 13-18

Author(s):

Young Imienwain Irivboje ◽

Adeboye Olusesan Fafiolu ◽

Muyideen Timothy Sanni ◽

Oluwabusayo Adewunmi Irivboje ◽

Christian Obiora Ndubuisi Ikeobi

Keyword(s):

Red Blood Cells ◽

Cell Volume ◽

Packed Cell Volume ◽

Blood Cells ◽

Rainy Season ◽

White Blood Cells ◽

Tropical Condition ◽

Mean Values ◽

Significant Difference ◽

Haematological Profile

AbstractHaematological studies help in understanding the relationship of blood characteristics to the environment. One of the major environmental factors influencing haematological indicators is season. This study was aimed at the variation in haematological profiles of two strains of laying birds at different seasons. We therefore evaluated the effects of genotype and season on the haematological profile in two strains of laying birds – Dominant brown (D-192) and Hyline brown in the tropics. A total of 50 birds per strain at 30 weeks of age were randomly selected for this study. Packed cell volume (PCV) and platelets were obtained using microhaematocrit whereas red blood cells (RBC) and white blood cells (WBC) were counted using a haemocytometer. The result obtained showed that there was a significant difference (P < 0.05) in the haematological profile of the birds in the two seasons compared (rainy and dry seasons). The haematological indices obtained during the rainy season had significantly higher mean values in packed cell volume (29.65 ± 0.32%), red blood cells (2.65 ± 0.03 × 1012/L), haemoglobin (100.1 ± 0.11 g/L) and eosinophil (2.5 ± 0.16%) while those obtained during the dry season had a higher significant effect on the white blood cells (19.16 ± 0.36 × 109/L). The results further revealed that between the strains, there was no significant difference (P > 0.05) in most of the indicators analysed but Hyline brown had significantly higher values (P < 0.05) of eosinophils and platelets. We concluded that the observed differences could be a result of genetic differences between the two strains of birds which gives room for selection and that the rainy season is more favourable for laying birds production under tropical condition.

Download Full-text

Evaluation of Hematocrit Level, Red Blood Cells and White Blood Cells Counts in Blood from Patients with Different Severities of Periodontal Diseases

Journal of Baghdad College of Dentistry ◽

10.26477/jbcd.v30i3.2523 ◽

2018 ◽

Vol 30 (3) ◽

pp. 1-6

Author(s):

Chinar J Ali ◽

Maha A Ahmed

Keyword(s):

Chronic Disease ◽

Red Blood Cells ◽

Blood Cells ◽

Periodontal Diseases ◽

Hematological Parameters ◽

White Blood Cells ◽

Control Group ◽

Pocket Depth ◽

Anemia Of Chronic Disease ◽

Mean Values

Background: Anemia of chronic disease (ACD) occurs in the presence of chronic infection, inflammatory conditions or neoplastic conditions despite of adequate iron and vitamins storage. Gingivitis is the inflammation of the gingiva, periodontitis is the inflammation in the periodontium that extend deeper with loss of connective tissue attachment and supporting bone. The main pathogenesis of periodontal diseases and ACD is immune activation. Aims of study: Determine and compare the clinical periodontal parameters (plaque index (PLI), gingival index (GI), bleeding on probing (BOP), probing pocket depth (PPD) and clinical attachment level (CAL)). Evaluate the hematocrit (Hct) level, red blood cells (RBCs) count and white blood cells (WBCs) count. Assess the correlations between the clinical periodontal parameters and hematological parameters at patients had gingivitis, chronic periodontitis (CP) with different severities (mild, moderate and severe) with healthy periodontium subjects. Materials and Methods: 35-50 years old, 150 male subjects were included in this study. They were divided into three study groups: group of 30 patients with gingivitis, group of 90 patients with CP which subdivided into (Mild CP=30 patients, Moderate CP =30 patients, Severe CP =30 patients) and control group 30 subjects with clinically healthy periodontium. Blood samples were collected then by automated blood analyzer the Hct, RBCs and WBCs were evaluated. Results: Comparisons among groups and subgroups revealed significant differences in Hct and WBCs, while RBCs was non-significant. Means values of RBCs count showed reduction in mild and severe CP subgroups. while, the Hct and WBCs mean values increased in patients with periodontal disease. The correlations between the clinical periodontal parameters with WBCs and RBCs were almost non-significant but, with Hct was mostly significant negative correlations. Conclusion: Inflammatory and immune responses in periodontal diseases caused change in different hematologic parameters which could contribute to the development of anemia of chronic disease.

Download Full-text

Antibody Response and Haematological Changes of Nigerian Locally Adapted Turkeys to Sheep Red Blood Cells (SRBC)

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Animal Science and Biotechnologies ◽

10.15835/buasvmcn-asb:2020.0005 ◽

2020 ◽

Vol 77 (2) ◽

pp. 20

Author(s):

Ayodele Emmanuel OGUNDERO ◽

Mofoyeke Oluwayemisi SANDA ◽

Adeyemi Sunday ADENAIKE ◽

Michael Irewole TAKEET ◽

Christian Obiora Ndubuisi IKEOBI

Keyword(s):

Red Blood Cells ◽

Antibody Titre ◽

Blood Cells ◽

White Blood Cells ◽

Primary Response ◽

Haematological Parameters ◽

High Antibody ◽

Sheep Red Blood Cells ◽

Antibody Titres ◽

High Antibody Titre

Haemagglutination assay and haematological analysis of 143 poults generated as F1 individuals by artificial insemination from randomly selected turkeys of White, Black and Lavender genotypes which are classified by antibody titre was carried out so as to confirm their antibody titre levels in response to sheep red blood cells (SRBC). Results showed that mean values obtained for high and low antibody titres were 7.31 and 2.67 respectively, resulting in the classification of the turkeys into Black high and low, Lavender high and black, and White high and low antibody titres. The genotype’s titre had significant (P <0.05) effect on the packed cell volume (PCV), haemoglobin (Hb), red blood cells (RBC), white blood cells (WBC) and basophil (BAS) of the basal haematological parameters. Genotype’s titre had no significant (P >0.05) effect on the primary response haematological parameters. Meanwhile, the primary response haematological parameters to SRBC antigen varied along the genotypes with the WBC increasing drastically in all the genotypes, signifying the presence of an antigen. The study concluded that the F1 turkey poult population studied diverged along the high and low antibody titre in response to SRBC. Thus, the F1 generation of the high antibody titre genotypes (Black high, White high and Lavender high) can be used as foundation stock for selection of local turkeys for high antibody titre.

Download Full-text

Changes in Haematological Parameters in Plasmodium falciparum Infected Malaria Patients in an Urban Slum of Lagos, Nigeria

Asian Journal of Biochemistry, Genetics and Molecular Biology ◽

10.9734/ajbgmb/2020/v5i430137 ◽

2020 ◽

pp. 20-29

Author(s):

U. O. Ozojiofor ◽

O. O. Bankole ◽

N. Anene ◽

A. U. Hassan ◽

S. A. Emaleku

Keyword(s):

Red Blood Cells ◽

Parasite Density ◽

Blood Cells ◽

Malaria Parasite ◽

Human Subjects ◽

White Blood Cells ◽

Urban Slum ◽

Haematological Parameters ◽

Distribution Width ◽

The Mean

The present study was carried to determine the changes in haematological parameters in P. falciparum infected patients in Ajeromi Ifelodun area of Lagos, Nigeria. Seventy (70) human subjects comprising of 50 P. falciparum malarial infected and 20 non-infected (control) subjects between 10-60 years were selected for this study. RDT test and microscopy were carried out to ascertain the presence of P. falciparum. They were grouped based on age criteria and level of parasitaemia. This work was carried out in the Department of Biochemistry, Nigeria Institute for Medical Research Laboratory, Yaba, Lagos, Nigeria between August 2016 and January 2017. Blood samples were collected for the determination of P. falciparum, level of parasitaemia and haematological parameters. Haematological parameters were determined using a Coulter A-T Pierce haematology analyzer (Beckman Coulter, Inc. Fullerton, CA, USA), P. falciparum was determined by rapid diagnostic test (RDT) and Microscopy. There was a significant increase in the mean level of total white blood cells (WBC) and red blood cells distribution width (RDW), and a significant decrease in the mean level of haematocrit (HCT), haemoglobin (HGB), red blood cells (RBC), and platelets (PLT) in the malaria infected patients than in the controls (p<0.05). There was also a higher malaria parasite density among malaria infected patients for ages above 20 and a lower malaria parasite density for ages below 20 in this study. The findings of this study show that infection with P. falciparum produces changes in haematological parameters in those infected and tested positive for malaria. The most commonly affected parameters are haemoglobin, haematocrit, white blood cells and platelet count.

Download Full-text

Effects of Age, Breed and Sex on Haematological Parameters of Growing Omani Goat Breeds

Sultan Qaboos University Journal for Science [SQUJS] ◽

10.24200/squjs.vol21iss2pp82-88 ◽

2016 ◽

Vol 21 (2) ◽

pp. 82

Author(s):

Nur El Huda I. Osman ◽

Rashid M. Al-Busaidi ◽

Eugene H. Johnson

Keyword(s):

Cell Volume ◽

Blood Cells ◽

White Blood Cells ◽

Haemoglobin Concentration ◽

Blood Parameters ◽

Haematological Parameters ◽

Mean Cell Volume ◽

Sultan Qaboos University ◽

Breed Differences ◽

Agricultural Experiment

Sixty seven healthy growing goat kids of three Omani breeds, born and raised in Sultan Qaboos University Agricultural Experiment Station, were used to study the effects of age, breed and sex on haematological parameters. Blood samples were collected at several times from the first to 25 weeks to study the following parameters: Red Blood Cells (RBCs), White Blood Cells (WBCs), Packed Cell Volume (PCV), Haemoglobin (Hb), Mean Cell Volume (MCV), Mean Cell Haemoglobin Concentration (MCHC) and Mean Cell Haemoglobin (MCH). At birth, MCV and MCH levels were significantly higher in Batina (BAT) kids than both Jabal Akhdar (JA) and Dhofari (DOF), but there were no breed differences in the rest of the haematological values. There were significant breed differences in RBC, MCH, WBC, MCV at different ages, with Hb and PCV being highly significant at all ages. All blood parameters changed with time but showed different patterns in all breeds. There was no effect of sex on RBC, MCHC, PCV, Hb or WBC, but female kids had significantly higher MCH and MCV values at week 12. This study indicates that haematology parameters in Omani goat kids could be affected by age, breed and sex.

Download Full-text