scholarly journals Prevalence, Biotyping, and Antimicrobial Resistance of Yersinia enterocolitica Isolated from Traditional Iranian Cheeses - Evaluation of Yersinia enterocolitica in Traditional Cheeses

2021 ◽  
Vol 78 (1) ◽  
pp. 122
Author(s):  
Milad TAVASSOLI ◽  
Abdollah JAMSHIDI ◽  
Golnaz RANJBAR ◽  
Mohammad Reza TORBATI MOGHADDAM ◽  
Asma AFSHARI
Keyword(s):  
Polymerase Chain Reaction ◽  
Antimicrobial Resistance ◽  
Yersinia Enterocolitica ◽  
Contamination Rate ◽  
Chain Reaction ◽  
Traditional Cheeses ◽  
Traditional Cheese ◽  
Selective Agar ◽  
Polymerase Chain

The present study aimed to investigate the contamination rate of various traditional Iranian cheese samples with Yersinia enterocolitica. In total, 200 cheese samples were collected from the northeast of Iran, and 10 types of traditional cheese were evaluated, including Lighvan, Kurdish, lactic, Tape-Salam, Onsory, Turkmen (type one and two), Sistani, Baluchi, and Kormange. The samples were analyzed using pre-enrichment Peptone-Sorbitol-Bile (PSB) broth, Yersinia selective agar (Cefsulodin-Irgasan-Novobiocin (CIN))following polymerase chain reaction (PCR). Antimicrobial tests were carried out using 13 antibiotics on all the positive samples. From the cheese samples collected from Khorasan Razavi province, Kurdish cheese had the highest contamination rate (9/20; 45%), while the lowest contamination rate was observed in Lighvan and Onsory cheese. Also, the most commonly identified biotype was biotype 1A (23/38; 61%). Y. entrocolitica was mostly susceptible to ciprofloxacin, tetracycline, gentamicin, chloramphenicol, cefotaxime, and ceftazidime, while resistant to ampicillin and amoxicillin.

scholarly journals Designing a time-effective TaqMan probe-based real-time polymerase chain reaction protocol for the identification of Yersinia enterocolitica in raw pork meat

2017 ◽  
Vol 86 (4) ◽  
pp. 317-323
Author(s):  
Milena Alicja Stachelska
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Yersinia Enterocolitica ◽  
Meat Products ◽  
Taqman Probe ◽  
Pork Meat ◽  
Chain Reaction ◽  
Microbial Risk ◽  
Polymerase Chain

The aim of this study was to design a time-effective method comprising a short pre-enrichment step in a non-selective broth in combination with the TaqMan probe applied in the real-time polymerase chain reaction to detectYersinia enterocoliticastrains in raw pork meat. The method enabled to detect 1 colony forming unit per 25 mg ofYersinia enterocoliticain pork meat. The specificity and reliability of the method was not diminished by the company of microflora naturally present in meat. The method was found successful to detect pathogenicYersinia enterocoliticastrains in pork meat. It is advised to be used for assessing the microbial risk and for controlling the microbial quality of meat and meat products.

scholarly journals Antimicrobial Resistance of Staphylococcus sp. Isolated from Cheeses

Animals ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 36
Author(s):  
Jana Výrostková ◽  
Ivana Regecová ◽  
František Zigo ◽  
Boris Semjon ◽  
Gabriela Gregová
Keyword(s):  
Polymerase Chain Reaction ◽  
Antimicrobial Resistance ◽  
Meca Gene ◽  
Common Species ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Chain Reaction ◽  
Coagulase Negative Staphylococci ◽  
Flight Mass Spectrometry ◽  
Polymerase Chain

S. aureus and some species of coagulase-negative staphylococci, including S. chromogenes and S. simulans, commonly cause intramammary infections. However, little attention was paid to the antimicrobial resistance of these species with respect to their occurrence in dairy products, for example, popular sheep and goat cheeses made from unpasteurized milk. The aim of this study was to investigate such sheep and goat cheeses for the occurrence and antimicrobial resistance of the relevant staphylococci species. The staphylococcal isolates were identified by polymerase chain reaction (130 isolates) and matrix assisted laser desorption/ionization time-of-flight mass spectrometry. The most common species of S. aureus (56 isolates) were identified, as well as S. chromogenes (16 isolates) and S. simulans (10 isolates). Antimicrobial resistance to penicillin, oxacilin, ceftaroline, teicoplanin, gentamicin, erythromycin, tetracycline and ofloxacin was subsequently determined in these species using the agar dilution method. The highest resistance was confirmed in all species, especially to penicillin (91%) and erythromycin (67%). The highest sensitivity was confirmed to ofloxacin (83%). Due to the high incidence of penicillin and oxacilin-resistant staphylococci, the mecA gene was detected by polymerase chain reaction, which was confirmed only in S. aureus isolates (19%). Our study shows that the tested strains (77%) were resistant to more than one antibiotic at a time.

scholarly journals Detection of Cholera Toxin-Producing Vibrio cholerae in Phytoplankton from Santubong and Samariang Estuaries

2019 ◽  
Vol 9 (1) ◽  
pp. 36-43
Author(s):  
Lesley Maurice Bilung ◽  
Mintra Prommani Etriam ◽  
Ahmad Syatir Tahar ◽  
Teng Sing Tung ◽  
Kasing Apun
Keyword(s):  
Polymerase Chain Reaction ◽  
Cholera Toxin ◽  
Vibrio Cholerae ◽  
Aquatic Environment ◽  
Physicochemical Parameters ◽  
Bile Salts ◽  
Chain Reaction ◽  
Selective Agar ◽  
Polymerase Chain ◽  
Number Of Bacteria

Many cholera outbreaks worldwide were associated with cholera toxin-producing Vibrio cholerae. The bacteria are ubiquitous in aquatic environment, whilst phytoplankton is associated with adaptation of the Vibrio species. This study was conducted to detect cholera toxin-producing Vibrio cholerae, and to determine association of the selected water physicochemical parameters with the number of the bacteria. In this study, a total of ten phytoplankton samples were collected at Santubong and Samariang Estuaries in Kuching, Sarawak. Water physicochemical parameters (temperature, pH and salinity) were recorded. Vibrio bacteria were cultivated on thiosulfate citrate bile-salts sucrose selective agar and analysed for cholera toxin-producing Vibrio cholerae using polymerase chain reaction by targeting ctxA gene that encodes for virulence cholera enterotoxin subunit A. The result revealed that a range of 1.0 × 107 – 8.0 × 107 CFU/ml of yellow colonies growing on the thiosulfate citrate bile-salts sucrose agars. Inversely, no samples were positive with cholera toxin-producing Vibrio cholerae. The physicochemical parameters at Samariang Estuary were more associated with the number of bacteria in the samples compared to Santubong Estuary.

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