scholarly journals Inhibitory Effects of Iso-α and β Hop Acids Against Pediococcus pentosaceus

2019 ◽  
Vol 47 (4) ◽  
pp. 1316-1322
Author(s):  
Delia MICHIU ◽  
Frank DELVIGNE ◽  
Nicolas MABON ◽  
Mirela JIMBOREAN ◽  
Melinda FOGARASI ◽  
...  
Keyword(s):  
Cell Viability ◽  
Incubation Period ◽  
Acid Stress ◽  
Digital Object Identifier ◽  
Inhibitory Effects ◽  
Pediococcus Pentosaceus ◽  
Selective Media ◽  
Ph Values ◽  
Short Incubation Period ◽  
Stress Changes

The goal of the research was to assess the inhibitory effects of  hop extracts, iso-α and β acids, against Pediococcus pentosaceus bacteria, during a short incubation period, both in liquid selective media (high pH values) and beer wort fermentation (low pH values) and testing if the identified iso-α acid stress changes the activity of S. cerevisiae boulardii yeast and ethanol production. Flow cytometry analysis was used for bacterial and yeast cell viability. In relation to the antibacterial activity of β-acids, a lower viability of Pediococcus pentosaceus cells was observed after a short incubation period in selective media, under iso-α acid stress. In beer wort, for a mixed culture with P. pentosaceus bacteria and S. cerevisiae boulardii yeast, under iso-α acid stress conditions at pH 4.0-5.0, Pediococcus pentosaceus exhibited lower cell viability (20.7%) than in selective media (61.4%). Regarding iso-α hop acid on S. cerevisiae boulardii yeast, the results showed that iso-α does not change the S. cerevisiae activity but prevents the culture from being contaminated by Pediococcus pentosaceus. The results highlighted reliable inhibitory effects of iso-α and β-acids against P. pentosaceus, both at pH 6.0-7.0 and pH 4.0-5.0, which open the possibility of hops being used as a supplement to prevent beverage contamination with spoilage microorganisms.   ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

Inhibition of Aflatoxin-Producing Fungi by Welsh Onion Extracts

1999 ◽  
Vol 62 (4) ◽  
pp. 414-417 ◽  
Author(s):  
J. J. FAN ◽  
J. H. CHEN
Keyword(s):  
Aspergillus Flavus ◽  
Mycelial Growth ◽  
Ethanol Extract ◽  
Aflatoxin Production ◽  
Inhibitory Effects ◽  
Welsh Onion ◽  
Extract Concentration ◽  
Ph Values ◽  
Ethanol Extracts ◽  
Yeast Extract Sucrose

Welsh onion ethanol extracts were tested for their inhibitory activity against the growth and aflatoxin production of Aspergillus flavus and A. parasiticus. The survival of spores of A. flavus and A. parasiticus depended on both the extract concentration and the exposure time of the spores to the Welsh onion extracts. The mycelial growth of two tested fungi cultured on yeast extract–sucrose broth was completely inhibited in the presence of the Welsh onion ethanol extract at a concentration of 10 mg/ml during 30 days of incubation at 25°C. The extracts added to the cultures also inhibited aflatoxin production at a concentration of 10 mg/ml or permitted only a small amount of aflatoxin production with extract concentration of 5 mg/ml after 2 weeks of incubation. Welsh onion ethanol extracts showed more pronounced inhibitory effects against the two tested aflatoxin-producing fungi than did the same added levels of the preservatives sorbate and propionate at pH values near 6.5.

A bat rabies isolate with an unusually short incubation period

1980 ◽  
Vol 33 (2) ◽  
pp. 211-222 ◽  
Author(s):  
G.M. Baer ◽  
A.K. Harrison ◽  
S.P. Bauer ◽  
J.H. Shaddock ◽  
F.A. Murphy
Keyword(s):  
Incubation Period ◽  
Short Incubation Period ◽  
Bat Rabies

Inhibitory effects of Panax ginseng glycoproteins in models of doxorubicin-induced cardiac toxicity in vivo and in vitro

2021 ◽  
Author(s):  
Yajun Chen ◽  
Lei Wang ◽  
Tianjia Liu ◽  
Zhidong Qiu ◽  
Ye Qiu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Cell Viability ◽  
Panax Ginseng ◽  
Cardiac Toxicity ◽  
H9c2 Cell ◽  
Inhibitory Effects ◽  
Myocardial Oxidative Stress

We investigated the protective effect of PGP against DOX-induced cardiotoxicity in vitro and in vivo. PGP increases H9C2 cell viability and inhibits apoptosis, alleviating DOX-induced myocardial oxidative stress-related cardiotoxicity.

scholarly journals Effect of dimethylmaleate and phorone on glutathione content of Setaria cervi (Nematoda: Filarioidea) during in vitro incubation

2007 ◽  
Vol 44 (1) ◽  
pp. 14-17 ◽  
Author(s):  
S. Gupta ◽  
N. Banu ◽  
A. Srivastava
Keyword(s):  
Drug Design ◽  
Incubation Period ◽  
Salt Solution ◽  
Glutathione Metabolism ◽  
Setaria Cervi ◽  
Short Incubation Period ◽  
In Vitro Incubation ◽  
Dose Dependent

AbstractGlutathione metabolism represents a prospective target for antifilarial drug design, and therefore, the alterations in glutathione (GSH) content of filarial worms by known mammalian GSH depletors i.e. dimethylmaleate (DMM) and phorone were first thought for investigation in model filarial worms Setaria cervi. The dose dependent GSH depletion was achieved when these worms were incubated at 37°C for 6 h in Hanks balanced salt solution with varying concentrations (10–250 μM) of DMM or phorone. During the short incubation period of 6 h, 250 μM of DMM and phorone declined more than 90 % of the GSH content of filarial worms.

scholarly journals The Shanghai Pasteur Institute

1901 ◽  
Vol 1 (2) ◽  
pp. 260-268
Author(s):  
Arthur Stanley
Keyword(s):  
Incubation Period ◽  
Human Subject ◽  
Local Condition ◽  
Pasteur Institute ◽  
Short Incubation Period

The idea of having the means of applying the Anti-rabic treatment of Pasteur in Shanghai, China, had its origin in the marked incidence of rabies among the numerous dogs of the Shanghai district and the consequent large mortality from this disease. The local condition was the more marked because of the short incubation period observed in Shanghai both in the human subject and in the rabbit inoculated from the rabid dog, being seldom much over a month in man and rarely over two weeks in rabbits inoculated subdurally.

scholarly journals Potential Probiotic Pediococcus pentosaceus M41 Modulates Its Proteome Differentially for Tolerances Against Heat, Cold, Acid, and Bile Stresses

2021 ◽  
Vol 12 ◽  
Author(s):  
Mohd Affan Baig ◽  
Mark S. Turner ◽  
Shao-Quan Liu ◽  
Anas A. Al-Nabulsi ◽  
Nagendra P. Shah ◽  
...  
Keyword(s):  
Acid Stress ◽  
Stress Conditions ◽  
Lactate Oxidase ◽  
Glycerol Phosphate ◽  
Pediococcus Pentosaceus ◽  
Sds Page ◽  
Tyrosine Protein Kinase ◽  
Imidazole Glycerol Phosphate ◽  
Proteomic Responses ◽  
Related Proteins

Probiotics containing functional food confer health benefits in addition to their nutritional properties. In this study, we have evaluated the differential proteomic responses of a potential novel probiotic Pediococcus pentosaceus M41 under heat, cold, acid, and bile stress conditions. We identified stress response proteins that could provide tolerances against these stresses and could be used as probiotic markers for evaluating stress tolerance. Pediococcus pentosaceus M41 was exposed for 2 h to each condition: 50°C (heat stress), 4°C (cold stress), pH 3.0 (acid stress) and 0.05% bile (bile stress). Proteomic analysis was carried out using 2D-IEF SDS PAGE and LC-MS/MS. Out of 60 identified proteins, 14 upregulated and 6 downregulated proteins were common among all the stress conditions. These proteins were involved in different biological functions such as translation-related proteins, carbohydrate metabolism (phosphoenolpyruvate phosphotransferase), histidine biosynthesis (imidazole glycerol phosphate synthase) and cell wall synthesis (tyrosine-protein kinase CapB). Proteins such as polysaccharide deacetylase, lactate oxidase, transcription repressor NrdR, dihydroxyacetone kinase were upregulated under three out of the four stress conditions. The differential expression of these proteins might be responsible for tolerance and protection of P. pentosaceus M41 against different stress conditions.

scholarly journals Inhibitory Effects of Indirubin Derivatives on the Growth of HL-60 Leukemia Cells

2010 ◽  
Vol 5 (1) ◽  
pp. 1934578X1000500
Author(s):  
Nguyen Manh Cuong ◽  
Bui Huu Tai ◽  
Dang Hoang Hoan ◽  
Tran Thu Huong ◽  
Young Ho Kim ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cancer Cells ◽  
Trypan Blue ◽  
Promyelocytic Leukemia ◽  
Leukemia Cells ◽  
Cytotoxic Activities ◽  
Inhibitory Effects ◽  
Trypan Blue Exclusion ◽  
Trypan Blue Exclusion Method ◽  
Exclusion Method

Six indirubin derivatives have been synthesized and their inhibitory effects on the growth of HL-60 human promyelocytic leukemia cells investigated. Cell viability was determined using the trypan blue exclusion method. Indirubin-3′-oxime (I-1) inhibited the growth of HL-60 cells with a GI50 value of 36.6 μM, whereas I-0, I-2, I-3, I-4 and I-6 showed only weak cytotoxic activities against HL-60 cancer cells with GI50 values in the range of 97.3 to over 100 μM. These results indicate that indirubin derivatives might be useful candidate agents for exploring potential antileukemic drugs.

A Case of Generalized Tetanus With a Short Incubation Period

2018 ◽  
pp. 64-66
Author(s):  
Fatih Temocin ◽  
◽  
Hatice Kose ◽  
Suleyman Sari ◽  
◽  
...  
Keyword(s):  
Incubation Period ◽  
Short Incubation Period
Sign in / Sign up

Export Citation Format

Share Document