scholarly journals Ameliorative Effect of Aqueous Leaf Extract of Moringa oleifera on Reproductive Function Following Cadmium Chloride Induced Oxidative Stress in Male Wistar Rats

2019 ◽  
Vol 11 (4) ◽  
pp. 352-357 ◽  
Author(s):  
Avwerosuoghene ODODO ◽  
Sunday O. OJEKA ◽  
Victor D. DAPPER
Keyword(s):  
Oxidative Stress ◽  
Cadmium Chloride ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Reproductive Function ◽  
Testicular Tissue ◽  
Cellular Damage ◽  
Male Wistar Rats ◽  
Aqueous Leaf Extract

Cadmium disrupts the blood-testes barrier, interferes with various antioxidant levels thus enhancing lipid peroxidation and ultimately leading to apoptosis and necrosis of testicular tissue. Moringa oleifera is a medicinal plant and a rich source of essential phytochemicals possessing antioxidant properties. The effect of aqueous leaf extract of M. oleifera on reproductive function following cadmium chloride induced oxidative stress in male Wistar rats was investigated. Forty adult male Wistar rats were assigned into five groups of eight rats each. Treatment was administered orally daily as follows: Group 1 (control): animal feed and tap water ad libitum; Group 2: 5 mg kg-1 cadmium chloride for 21 days; Group 3: 500 mg kg-1 of M. oleifera and 5 mg kg-1 of cadmium chloride for 21 days; Group 4: 5 mg kg-1 cadmium chloride for 21 days followed by 500 mg kg-1 M. oleifera for the next 35 days; Group 5: 5 mg kg-1 cadmium chloride for 21 days followed by 750 mg kg-1 M. oleifera for the next 35 days. At the end of treatment, blood was obtained by direct cardiac puncture for fertility hormone assay and testicular tissue specimens were harvested for semen analysis and determination of antioxidant levels. Results obtained indicated that rats treated with the various extracts had significantly increased superoxide dismutase, malondialdehyde and catalase levels, increased serum concentrations of testosterone, follicle stimulating hormone and luteinizing hormone and increased percentage of viable and normal spermatozoa compared to control and only cadmium chloride treated rats (p < 0.05). The results obtained suggest that treatments with M. oleifera extract could ameliorate possible cellular damage caused by administration of cadmium chloride.

scholarly journals Effects of Aqueous Leaf Extract of Simarouba glauca DC (Simaroubaceae) on Lipoprotein Homeostasis and Oxidative Stress Biomarkers

2021 ◽  
Vol 1 (1) ◽  
pp. 20-29
Author(s):  
Sammydavies E. Osagie-Eweka ◽  
Noghayin J. Orhue ◽  
Eric I. Omogbai
Keyword(s):  
Oxidative Stress ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Biologically Active ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Male Wistar Rats ◽  
Aqueous Leaf Extract ◽  
Simarouba Glauca ◽  
And Oxidative Stress

Background and Purpose: Simarouba glauca is widely reported to contain a number of biologically active compounds with potentials in the treatment of numerous diseases. The study was conducted to evaluate the sub-acute effects of the aqueous leaf extract of Simarouba glauca (AESG) on lipoproteins and oxidative stress biomarkers in male Wistar rats. Methods: Oral administration of AESG was carried out in line with the guidelines of the Organization for Economic Co-operation and Development (OECD), No. 425 using a total of 24 male Wistar rats allotted to four groups (n=6); given distilled water, 500, 1000, and 2000 mg/kg/day of AESG respectively for 30 days. Results: In plasma, there was a significant reduction (P?0.05) in HDL-cholesterol; elevated (P?0.05) triglycerides (TG) at 1000 and 2000 mg/kg/day; elevated (P?0.05), and LDL-cholesterol at 500 and 1000 mg/kg/day, relative to the control. While the level of liver total cholesterol (TC) reduced significantly, it increased in the heart. Catalase (CAT) activity in the liver increased significantly (P?0.05) at all doses. The dose of 1000 mg/kg/day significantly (P?0.05) elevated kidney CAT activity. The activities of superoxide dismutase (SOD) in liver and heart reduced (P?0.05) at 500 mg/kg/day. At all doses, the levels of reduced glutathione (GSH) in plasma, liver and heart were comparable with the control. Although, there were no significant changes in plasma and liver glutathione peroxidase (GSH-PX) activity at all doses, animals given 500 mg/kg had reduction (P?0.05) in the heart GSH-PX activity compared to the control. Conclusion: Oral sub-acute AESG at high doses altered lipid homeostasis in plasma and heart without lipid peroxidation or oxidative stress. The extract has the potential to cause hyperlipidemia.

Adansonia digitata L. leaf extract attenuates lead-induced cortical histoarchitectural changes and oxidative stress in the prefrontal cortex of adult male Wistar rats

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Vivian Atuadu ◽  
Ben-Azu Benneth ◽  
John Oyem ◽  
Emmanuel Esom ◽  
Chris Mba ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Prefrontal Cortex ◽  
Lead Acetate ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Neurological Diseases ◽  
Lead Toxicity ◽  
Adansonia Digitata ◽  
Male Wistar Rats ◽  
Aqueous Leaf Extract

AbstractObjectivesAdansonia digitata L. is popularly known for the management of various neurological diseases in ethno-medicine. Studies have shown that lead toxicity is a possible risk factor for early onset of neurodegenerative disease. Hence, this study was designed to evaluate the effect of A. digitata aqueous leaf extract (ADALE) against lead-induced oxidative stress and histo-architectural changes in the prefrontal cortex of adult Wistar rats.MethodsSaline (10 mL/kg), ADALE (500 and 1000 mg/kg) and EDTA (55 mg/kg) were pretreated orally 30 min prior to lead acetate (LA) (120 mg/kg) administration to male Wistar rats (n=7) for 21 days. Thereafter, standard biochemical (superoxide dismutate, catalase, glutathionxe and malondialdehyde), histological (H&E) and histochemical assessment (crystyl fast violet stain for nissil substance) were carried out in the prefrontal cortex.ResultsADALE significantly (p<0.05) reversed LA-induced oxidative stress, as evidenced by increased catalase, superoxide dismutase and oxidized glutathione levels, and decreased malondialdehyde concentration in the prefrontal cortex. Also, the increase chromatolysis and neuronal pyknosis of the pyramidal neurons of the prefrontal cortex were significantly attenuated by ADALE.ConclusionsThe result of this study showed that A. digitata aqueous leaf extract attenuated lead acetate-induced cortical neurodegeneration via inhibition of oxidative stress.

Attenuation of Lead-Induced Oxidative Stress in Rat Brain, Liver, Kidney and Blood of Male Wistar Rats by Moringa Oleifera Seed Powder

2014 ◽  
Vol 33 (4) ◽  
pp. 323-337 ◽  
Author(s):  
Manoj Kumar Velaga ◽  
Lucius K. Daughtry ◽  
Angelica C. Jones ◽  
Prabhakara Rao Yallapragada ◽  
Sharada Rajanna ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rat Brain ◽  
Wistar Rats ◽  
Moringa Oleifera ◽  
Male Wistar Rats ◽  
Moringa Oleifera Seed

scholarly journals Reproductive Function Evaluation in Female Wistar Rats Treated with Aqueous leaf Extract of Cissusaralioides

2019 ◽  
Vol 16 (04) ◽  
pp. 857-864
Author(s):  
Bartholomew Chukwuebuka Nwogueze ◽  
Anthony Emeka Ojieh ◽  
Richard Nduka Ossai ◽  
ChidinmaNwanneamaka Eke
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Reproductive Function ◽  
Function Evaluation ◽  
Eastern Region ◽  
Body Weight Reduction ◽  
Therapeutic Benefits ◽  
The Family ◽  
Female Wistar Rats ◽  
Aqueous Leaf Extract

ABSTRACT: Background: Medicinal plants are increasingly getting recognition. Cissus aralioides is a lofty climber belonging to the family of Vitaceae and widely distributed in South-Eastern region of Nigeria. Varied therapeutic benefits as well as well health harming effects have been reported about the plant. This study evaluated the effects of aqueous extract of Cissus aralioides leaf on reproductive functions in female Wistar rats.Methods: 72 adult female Wistar rats weighing between 110-180/g were used for the study. The animals were randomly grouped into 6 groups of twelve rats each (n=12). Groups 1 and 2 served as control while groups 3 to 6 were the experimental groups and were administered (100/mg, 200/mg, 300/mg and 500/mg)/kg of the extract respectively for 4 weeks. At the end of the experiment, blood samples were collected and centrifuged to obtain the serum for biochemical analysis for Follicle Stimulating Hormone (FSH), Estrogen and Luteinizing Hormone (LH). Data were analyzed using the SPSS package and expressed as mean± SEM and ANOVA.Results: There was significant body weight reduction across groups administered with the extract. Also, there were significant decrease in estrogen level in groups that received 300/mg/kg and 500/mg/kg when compared with the control groups, a similar decrease was not observed with LH and FSH across the groups.Conclusion: Observations from this study shows that aqueous leaf extract of Cissus aralioides decreases circulating levels of estrogen in female Wistar rats.

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