Adansonia digitata L. leaf extract attenuates lead-induced cortical histoarchitectural changes and oxidative stress in the prefrontal cortex of adult male Wistar rats

AbstractObjectivesAdansonia digitata L. is popularly known for the management of various neurological diseases in ethno-medicine. Studies have shown that lead toxicity is a possible risk factor for early onset of neurodegenerative disease. Hence, this study was designed to evaluate the effect of A. digitata aqueous leaf extract (ADALE) against lead-induced oxidative stress and histo-architectural changes in the prefrontal cortex of adult Wistar rats.MethodsSaline (10 mL/kg), ADALE (500 and 1000 mg/kg) and EDTA (55 mg/kg) were pretreated orally 30 min prior to lead acetate (LA) (120 mg/kg) administration to male Wistar rats (n=7) for 21 days. Thereafter, standard biochemical (superoxide dismutate, catalase, glutathionxe and malondialdehyde), histological (H&E) and histochemical assessment (crystyl fast violet stain for nissil substance) were carried out in the prefrontal cortex.ResultsADALE significantly (p<0.05) reversed LA-induced oxidative stress, as evidenced by increased catalase, superoxide dismutase and oxidized glutathione levels, and decreased malondialdehyde concentration in the prefrontal cortex. Also, the increase chromatolysis and neuronal pyknosis of the pyramidal neurons of the prefrontal cortex were significantly attenuated by ADALE.ConclusionsThe result of this study showed that A. digitata aqueous leaf extract attenuated lead acetate-induced cortical neurodegeneration via inhibition of oxidative stress.

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The effect of aqueous leaf extract of Adansonia digitata (baobab) on diabetes mellitus and the anterior pituitary of adult male wistar rats

Journal of Diabetes and Endocrinology ◽

10.5897/jde2019.0131 ◽

2019 ◽

Vol 10 (3) ◽

pp. 18-29

Author(s):

Pamela Okorie ◽

Francis Agu ◽

Celestine Ani ◽

Ifeoma Alozie ◽

Choice Nworgu ◽

...

Keyword(s):

Diabetes Mellitus ◽

Adult Male ◽

Anterior Pituitary ◽

Wistar Rats ◽

Leaf Extract ◽

Adansonia Digitata ◽

Male Wistar Rats ◽

Aqueous Leaf Extract

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Effects of Aqueous Leaf Extract of Simarouba glauca DC (Simaroubaceae) on Lipoprotein Homeostasis and Oxidative Stress Biomarkers

Pharmacology and Toxicology of Natural Medicines (ISSN: 2756-6838) ◽

10.52406/ptnm.v1i1.12 ◽

2021 ◽

Vol 1 (1) ◽

pp. 20-29

Author(s):

Sammydavies E. Osagie-Eweka ◽

Noghayin J. Orhue ◽

Eric I. Omogbai

Keyword(s):

Oxidative Stress ◽

Wistar Rats ◽

Leaf Extract ◽

Biologically Active ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

Male Wistar Rats ◽

Aqueous Leaf Extract ◽

Simarouba Glauca ◽

And Oxidative Stress

Background and Purpose: Simarouba glauca is widely reported to contain a number of biologically active compounds with potentials in the treatment of numerous diseases. The study was conducted to evaluate the sub-acute effects of the aqueous leaf extract of Simarouba glauca (AESG) on lipoproteins and oxidative stress biomarkers in male Wistar rats. Methods: Oral administration of AESG was carried out in line with the guidelines of the Organization for Economic Co-operation and Development (OECD), No. 425 using a total of 24 male Wistar rats allotted to four groups (n=6); given distilled water, 500, 1000, and 2000 mg/kg/day of AESG respectively for 30 days. Results: In plasma, there was a significant reduction (P?0.05) in HDL-cholesterol; elevated (P?0.05) triglycerides (TG) at 1000 and 2000 mg/kg/day; elevated (P?0.05), and LDL-cholesterol at 500 and 1000 mg/kg/day, relative to the control. While the level of liver total cholesterol (TC) reduced significantly, it increased in the heart. Catalase (CAT) activity in the liver increased significantly (P?0.05) at all doses. The dose of 1000 mg/kg/day significantly (P?0.05) elevated kidney CAT activity. The activities of superoxide dismutase (SOD) in liver and heart reduced (P?0.05) at 500 mg/kg/day. At all doses, the levels of reduced glutathione (GSH) in plasma, liver and heart were comparable with the control. Although, there were no significant changes in plasma and liver glutathione peroxidase (GSH-PX) activity at all doses, animals given 500 mg/kg had reduction (P?0.05) in the heart GSH-PX activity compared to the control. Conclusion: Oral sub-acute AESG at high doses altered lipid homeostasis in plasma and heart without lipid peroxidation or oxidative stress. The extract has the potential to cause hyperlipidemia.

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Ameliorative Effect of Trevo Dietary Supplement Against Lead Acetate Nephrotoxicity

Iranian Jornal of Toxicology ◽

10.32598/ijt.16.1.857.1 ◽

2022 ◽

Vol 16 (1) ◽

pp. 34-42

Author(s):

Omotayo B. Ilesanmi ◽

◽

Eni-yimini Solomon Agoro ◽

Keyword(s):

Oxidative Stress ◽

Uric Acid ◽

Lead Acetate ◽

Wistar Rats ◽

Antioxidant Properties ◽

Kidney Tissue ◽

Lead Toxicity ◽

Serum Levels ◽

Tissue Samples ◽

Male Wistar Rats

Background: Trevo is a nutritional supplement with numerous bioactive natural products, with detoxifying and antioxidant properties. The purpose of this study was to investigate the ability of Trévo to protect against oxidative stress induced by lead in the kidneys of male Wistar rats. Methods: Thirty-five healthy male Wistar rats were divided into five groups of seven rats each, using a randomized design. I=control; II=15 mg/kg of lead acetate (PbA); III=2 ml/kg of trevo+PbA; IV=5 ml/kg of trevo+PbA; V=5 ml/kg of trevo. Animals were treated with trevo for five days before co-administration with lead intraperitoneally for 10 consecutive days. Animals were sacrificed 24hr after the last administration, blood samples were collected via cardiac puncture, and processed for assessment of urea, creatinine, and uric acid (UA), while the kidney samples were excised and processed for the following biochemical assays: Malondialdehyde (MDA), Glutathione-S-Transferase (GST), Catalase (CAT), Superoxide Dismutase (SOD), and Reduced Glutathione (GSH). Results: Injection of PbA caused a significant increase in the serum levels of urea, creatinine, and uric acid, and a significant increase (P<0.001) in the MDA concentration, and decreases in GSH concentration, CAT, SOD, and GST activities (P<0.05) as compared to the controls. Pretreatment with trevo prevented the oxidative stress induced by lead acetate in the kidney tissue samples and improve the renal function. The protective effect was evident at 5 ml/kg of trevo. Conclusion: The results showed that trevo was nephroprotective against lead toxicity and the activity might be linked to the presence of numerous antioxidant phytochemicals present in trevo.

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Ameliorative Effect of Aqueous Leaf Extract of Moringa oleifera on Reproductive Function Following Cadmium Chloride Induced Oxidative Stress in Male Wistar Rats

Notulae Scientia Biologicae ◽

10.15835/nsb11410485 ◽

2019 ◽

Vol 11 (4) ◽

pp. 352-357 ◽

Cited By ~ 1

Author(s):

Avwerosuoghene ODODO ◽

Sunday O. OJEKA ◽

Victor D. DAPPER

Keyword(s):

Oxidative Stress ◽

Cadmium Chloride ◽

Wistar Rats ◽

Leaf Extract ◽

Moringa Oleifera ◽

Reproductive Function ◽

Testicular Tissue ◽

Cellular Damage ◽

Male Wistar Rats ◽

Aqueous Leaf Extract

Cadmium disrupts the blood-testes barrier, interferes with various antioxidant levels thus enhancing lipid peroxidation and ultimately leading to apoptosis and necrosis of testicular tissue. Moringa oleifera is a medicinal plant and a rich source of essential phytochemicals possessing antioxidant properties. The effect of aqueous leaf extract of M. oleifera on reproductive function following cadmium chloride induced oxidative stress in male Wistar rats was investigated. Forty adult male Wistar rats were assigned into five groups of eight rats each. Treatment was administered orally daily as follows: Group 1 (control): animal feed and tap water ad libitum; Group 2: 5 mg kg-1 cadmium chloride for 21 days; Group 3: 500 mg kg-1 of M. oleifera and 5 mg kg-1 of cadmium chloride for 21 days; Group 4: 5 mg kg-1 cadmium chloride for 21 days followed by 500 mg kg-1 M. oleifera for the next 35 days; Group 5: 5 mg kg-1 cadmium chloride for 21 days followed by 750 mg kg-1 M. oleifera for the next 35 days. At the end of treatment, blood was obtained by direct cardiac puncture for fertility hormone assay and testicular tissue specimens were harvested for semen analysis and determination of antioxidant levels. Results obtained indicated that rats treated with the various extracts had significantly increased superoxide dismutase, malondialdehyde and catalase levels, increased serum concentrations of testosterone, follicle stimulating hormone and luteinizing hormone and increased percentage of viable and normal spermatozoa compared to control and only cadmium chloride treated rats (p < 0.05). The results obtained suggest that treatments with M. oleifera extract could ameliorate possible cellular damage caused by administration of cadmium chloride.

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