scholarly journals Callus induction and chemical characterization of cell suspension cultures of jojoba (Simmondsia chinensis L.)

2021 ◽  
Vol 6 (3) ◽  
pp. 5
Author(s):  
Muhammad Akram ◽  
Faheem Aftab
Keyword(s):  
Cell Viability ◽  
Cell Suspension ◽  
Leaf Explants ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Cell Counting ◽  
Naphthalene Acetic Acid ◽  
Simmondsia Chinensis ◽  
Triphenyltetrazolium Chloride ◽  
Efficient System

Jojoba (Simmondsia chinensis L.) oil is also known as liquid wax or fixed oil. It is an important metabolite of jojoba having commercial importance in cosmetics as well as a potential biofuel source. We presented an efficient system for in vitro establishment of cell suspension cultures (CSC) from proliferating friable calluses. For this purpose, cotyledon, internode, and leaf explants were cultured on MS medium + 1, 2, 4, 6, 8 or 10 µM 2, 4-Dichlorophenoxyacetic acid (2, 4-D), α-Naphthalene acetic acid (NAA) alone or in combination with 1 or 2 µM N6-benzylaminopurine (BAP) or Kinetin. Results demonstrated that 100% healthy, friable and variegated calluses were obtained on 8 µM, 10 µM 2, 4-D or 2, 4-D 10 µM + 2 µM BAP and represented as callus lines (CL) CL-1, CL-2 or CL-3, respectively, after 38 days. One-gram callus tissue per CL was then immersed in the respective liquid medium and agitated on an orbital shaker at 60-70 rpm under the growth room conditions (25 ± 2 °C, 16 h light period) for the preparation of CSC. After 15 days, CSC was sieved and large clumps were removed. Growth measurement of CSC was determined by cell counting, packed cell volume (PCV) and cell viability. The highest number of viable cells was obtained at 2.57 OD with CL-3, where PCV was highest (0.35 ml) on CL-1 of 38 days old calluses. 2,3,5-Triphenyltetrazolium chloride was a reliable approach for the determination of cell viability of CSC.

Effects of proline on cell viability and hCTLA4Ig production in transgenic rice cell suspension cultures

2008 ◽  
Vol 136 ◽  
pp. S138-S139
Author(s):  
Mi-Na Song ◽  
Su-Hwan Cheon ◽  
Jung-Ae Lim ◽  
Hyun-Cheol Park ◽  
Dong-Il Kim
Keyword(s):  
Cell Viability ◽  
Cell Suspension ◽  
Transgenic Rice ◽  
Cell Suspension Cultures ◽  
Suspension Cultures

Characterization and plant regeneration of cell suspension cultures of Lycopersicon chilense

1991 ◽  
Vol 69 (10) ◽  
pp. 2257-2260 ◽  
Author(s):  
Ann Francine Greer ◽  
Zohreh Tabaeizadeh
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
Casein Hydrolysate ◽  
Leaf Explants ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Cell Suspensions ◽  
Lag Phase ◽  
Petiole Explants ◽  
Lycopersicon Chilense

To produce calli for the establishment of a cell suspension, leaf, stem, and petiole explants of Lycopersicon chilense Dun., grown in vitro and in the soil, were cultured on media containing 15 different combinations of benzylaminopurine, kinetin, and indole acetic acid. Among the three types of tissues, leaf explants showed the best response. Cell suspension cultures of L. chilense were established from leaf callus derived from soil grown plants using Murashige and Skoog's medium supplemented with casein hydrolysate (250 mg/L), coconut water (5%), and 2,4-dichlorophenoxyacetic acid (2 mg/L). Once established, cell suspensions showed a rapid growth rate with no marked lag phase. Shooting via organogenesis occurred from callus derived from cell suspensions on medium containing 2 mg/L benzylaminopurine. Regenerated plants had the same morphology as the original plants. Key words: Lycopersicon chilense, tomato, tissue culture, cell suspensions, organogenesis, plant regeneration.

Establishment of Callus and Cell Suspension Cultures of Corydalis saxicola Bunting, a Rare Medicinal Plant

2006 ◽  
Vol 61 (3-4) ◽  
pp. 251-256 ◽  
Author(s):  
Hua Cheng ◽  
Long-Jiang Yu ◽  
Qiong-Yue Hu ◽  
Shan-Cai Chen ◽  
You-Ping Sun
Keyword(s):  
Cell Suspension ◽  
Callus Induction ◽  
Large Scale ◽  
Callus Formation ◽  
Leaf Explants ◽  
Cell Suspension Cultures ◽  
Biomass Accumulation ◽  
Suspension Cultures ◽  
Inoculum Size ◽  
Enhanced Production

An efficient procedure has been developed for callus induction and cell suspension cultures of C. saxicola for the first time. Explant selection was carried out among leaf, stem and root to select a suitable type of explants capable of higher callus formation. Leaf explants thus selected showed maximum response to callus induction (67.1%). Modified B5 medium supplemented with 0.5 mg l−1 2,4-D plus 2 mg l−1 BA was the most favorable medium for callus formation with the highest induction rate (94.8%) and greatest fresh weight of callus (1.7 g per explant). Cell suspension cultures were established by transferring 2-8 g fresh callus to 80 ml liquid B5 medium. An inoculum size of 8 g produced the greatest biomass accumulation, dehydrocavidine and berberine productions, which was 13.1 g l−1, 8.0 mg l−1 and 4.1 mg l−1, respectively. In response to various sucrose concentrations from 10 g l−1 to 80 g l−1, cultures with 60 g sucrose l−1 not only produced the highest dry biomass (18.5 g l−1) but also the highest formation of dehydrocavidine (11.6 mg l−1) and berberine (7.6 mg l−1). These prepared cell suspension cultures provided a useful material for further regulation of alkaloid biosynthesis and for enhanced production of valuable alkaloids on a large scale.

Effects of substitute carbon sources on cell viability and hCTLA4Ig productivity in transgenic rice cell suspension cultures

2008 ◽  
Vol 136 ◽  
pp. S159-S160
Author(s):  
Hyun-Cheol Park ◽  
Su-Hwan Cheon ◽  
Jun-Young Kwon ◽  
Mi-Na Song ◽  
Ji-Yoen Han ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cell Suspension ◽  
Transgenic Rice ◽  
Carbon Sources ◽  
Cell Suspension Cultures ◽  
Suspension Cultures

scholarly journals Cell Culture of Bursera Linanoe in a Stirred Tank Bioreactor for Production of Linalool and Linalyl Acetate

2017 ◽  
Vol 12 (3) ◽  
pp. 1934578X1701200
Author(s):  
Leticia Pavón-Reyes ◽  
Silvia Evangelista-Lozano ◽  
Gabriela Sepúlveda-Jiménez ◽  
Víctor Chávez Ávila ◽  
Mario Rodríguez-Monroy
Keyword(s):  
Cell Viability ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Stirred Tank ◽  
Phase Cell ◽  
Naphthalene Acetic Acid ◽  
Linalyl Acetate ◽  
Stirred Tank Bioreactor ◽  
Maximum Biomass ◽  
Murashige And Skoog Medium

Bursera linanoe cell suspension cultures were initiated from callus grown in Murashige and Skoog medium supplemented with naphthalene acetic acid (3.0 mg L−1) and 6-benzylaminopurine (0.5 mg L−1). In flasks, B. linanoe cell cultures grew over a 9 day period, reaching a maximum biomass of 11.16 g DW L−1. Throughout the growth phase, cell viability was constant at 60 – 70%. In contrast, B. linanoe cells growing in a bioreactor achieved a maximum biomass of 22.26 g DW L−1 (after 7 days), and cell viability was constant at 75 - 85%. Production of linalool and linalyl acetate in the bioreactor (3.02 and 2.40 mg g−1 DW, respectively) was significantly greater than that achieved from cells in flask cultures (1.05 and 0.97 mg g−1 DW, respectively). B. linanoe cell suspension culture has potential as an alternative method for the production of essential oils.

scholarly journals Comparison of Cuminaldehyde Contents from Cell Suspension Cultures and Seeds of [Bunium persicum (Boiss.) B. Fedtsch.]

2012 ◽  
Vol 4 (4) ◽  
pp. 49-54 ◽  
Author(s):  
Sara KHOSRAVINIA ◽  
Seyed Mahdi ZIARATNIA ◽  
Abdolreza BAGHERI ◽  
Ghadir RAJABZADEH ◽  
Seyed Hassan MARASHI
Keyword(s):  
Gas Chromatography ◽  
Acetic Acid ◽  
Cell Suspension ◽  
Supercritical Fluid ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Naphthalene Acetic Acid ◽  
Chromatography Analysis ◽  
Bunium Persicum ◽  
Dichlorophenoxy Acetic Acid

The cell suspension culture and seed samples of Bunium persicum were extracted by supercritical fluid, hydrodistillation and solvent methods and analyzed by Gas Chromatography. In this study to compare the different methods of extractions, cuminaldehyde was targeted as one of the Black zira essential oil constitute. For callus induction the germinated seeds were cultured as explants on Murashige and Skoog medium supplemented with 2 mg/l 2,4-dichlorophenoxy acetic acid and 0.5 mg/l kinetin (treatment A) as well as 2 mg/l ?-naphthalene acetic acid and 0.5 mg/l 6-benzyl aminopurine (treatment B) and followed by cells suspension cultures establishment for the first time. The results of cell culture showed that cells from treatment B have a growth rate higher than A. All extracts were dissolved in 1 ml hexane and analyzed by Gas Chromatography. According to the Gas Chromatography analysis, cuminaldehyde was not detected in the supercritical fluid samples, while it was present in hydrodistillation and solvent extract. Cuminaldehyde percentage in cell and seed solvent extracts was 4.65% and 18.61% respectively. Gas Chromatography results also showed that no cuminaldehyde is present in media extracts, means no cuminaldehyde has been secreted into the medium.

scholarly journals Rosmarinic acid production in cell suspension cultures of Ehretia asperula Zollinger & Moritz

2022 ◽  
Vol 9 (1) ◽  
pp. 70-75
Author(s):  
Pham Thi My Tram ◽  
Ngo Ke Suong ◽  
Le Thi Thuy Tien
Keyword(s):  
Cell Suspension ◽  
Rosmarinic Acid ◽  
Rotation Speed ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Inoculum Size ◽  
Naphthalene Acetic Acid ◽  
Callus Line ◽  
Pharmaceutical Industries

Plant cell cultures provide an alternative means for producing secondary compounds in food, cosmetic and pharmaceutical industries. Ehretia asperula Zollinger & Moritzi is used as a traditional medicine for the treatment of liver detoxification, ulcers, tumors, inflammation and enhancing the body's resistance in Vietnam. The study was carried out to select suitable callus line for cell suspension cultures of E. asperula Zollinger & Moritzi and investigate the effects of inoculum size, rotation speed and naphthalene acetic acid (NAA) on the proliferation of cell suspension cultures. In addition, the influence of light intensity on the growth and rosmarinic acid (RA) biosynthesis of cell suspension was also surveyed. After 4 weeks of culture, the white to pale yellow friable callus expanded significantly with a fresh weight (FW) of 0.788 g and a high RA content of 2.062 mg/g FW. An appropriate medium for cell proliferation was the liquid B5 medium, which contained 30 g/l glucose, 0.1 mg/l benzyl adenine (BA) and 0.4 mg/l NAA. The results also demonstrated that a 1:20 ratio (w/v) inoculum size, darkness and rotation speed of 90 rpm were the optimal conditions for the proliferation and RA accumulation to 188.217 mg/l in 4 weeks of culture. These findings showed that E. asperula Zollinger & Moritzi cell suspension cultures could be a potential alternative approach for RA production in vitro.

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