Low Temperature Is Critical for Jujube Grafting Success in Frost-prone Northern New Mexico

The increased popularity of jujube (Ziziphus jujuba) combined with the difficulty of grafting have limited supplies of grafted trees in the United States. From 2011 to 2020, grafting was practiced for cultivar amplification after importation and cultivar trials in frost-prone northern New Mexico. Grafting success was related to not only grafting techniques but also climate factors. Bark grafting, whip/tongue grafting, and cleft grafting were commonly used in nurseries. Low temperatures had a critical role in jujube grafting success in marginal regions and were more important than the grafting technique. If frost occurs before or near the leafing time, then grafting should be delayed until the rootstocks are determined to be healthy and alive. If frost occurs after grafting, then grafting failure and/or thin and small plant percentages increased. If only branchlets appear after grafting, then pinching branchlets could stimulate new shoot growth.

CryoEM analysis of small plant biocatalysts at sub-2 Å resolution

Enzyme catalysis has emerged as a key technology for developing efficient, sustainable processes in the chemical, biotechnological and pharmaceutical industries. Plants provide large and diverse pools of biosynthetic enzymes that facilitate complex reactions, such as the formation of intricate terpene carbon skeletons, with exquisite specificity. High-resolution structural analysis of these enzymes is crucial in order to understand their mechanisms and modulate their properties by targeted engineering. Although cryo-electron microscopy (cryoEM) has revolutionized structural biology, its applicability to high-resolution structural analysis of comparatively small enzymes has so far been largely unexplored. Here, it is shown that cryoEM can reveal the structures of plant borneol dehydrogenases of ∼120 kDa at or below 2 Å resolution, paving the way for the rapid development of new biocatalysts that can provide access to bioactive terpenes and terpenoids.

There Is No ‘Rule of Thumb’: Genomic Filter Settings for a Small Plant Population to Obtain Unbiased Gene Flow Estimates

The application of high-density polymorphic single-nucleotide polymorphisms (SNP) markers derived from high-throughput sequencing methods has heralded plenty of biological questions about the linkages of processes operating at micro- and macroevolutionary scales. However, the effects of SNP filtering practices on population genetic inference have received much less attention. By performing sensitivity analyses, we empirically investigated how decisions about the percentage of missing data (MD) and the minor allele frequency (MAF) set in bioinformatic processing of genomic data affect direct (i.e., parentage analysis) and indirect (i.e., fine-scale spatial genetic structure – SGS) gene flow estimates. We focus specifically on these manifestations in small plant populations, and particularly, in the rare tropical plant species Dinizia jueirana-facao, where assumptions implicit to analytical procedures for accurate estimates of gene flow may not hold. Avoiding biases in dispersal estimates are essential given this species is facing extinction risks due to habitat loss, and so we also investigate the effects of forest fragmentation on the accuracy of dispersal estimates under different filtering criteria by testing for recent decrease in the scale of gene flow. Our sensitivity analyses demonstrate that gene flow estimates are robust to different setting of MAF (0.05–0.35) and MD (0–20%). Comparing the direct and indirect estimates of dispersal, we find that contemporary estimates of gene dispersal distance (σrt = 41.8 m) was ∼ fourfold smaller than the historical estimates, supporting the hypothesis of a temporal shift in the scale of gene flow in D. jueirana-facao, which is consistent with predictions based on recent, dramatic forest fragmentation process. While we identified settings for filtering genomic data to avoid biases in gene flow estimates, we stress that there is no ‘rule of thumb’ for bioinformatic filtering and that relying on default program settings is not advisable. Instead, we suggest that the approach implemented here be applied independently in each separate empirical study to confirm appropriate settings to obtain unbiased population genetics estimates.

Sibbaldia procumbens: An icon redrawn

A new cover has been created for Sibbaldia the International Journal of Botanic Garden Horticulture to commemorate the 350 Anniversary of the Royal Botanic Garden Edinburgh (RBGE) in 2020. The Sibbald Trust, which supports the work of the Garden, agreed to fund the commissioning of a botanical drawing of the alpine species Sibbaldia procumbens L. to be used as the cover image for volume 20 and subsequent volumes. The drawing and accompanying colour wash overlay will become part of the RBGE Florilegium - a collection of botanical drawings that form a visual record of the Living Collection and a reflection of the scientific and horticultural interests of the institute. This article describes the process of creating a painting of a small plant with minute details. Colour images of the plant and the drawings are also reproduced.

Mobius Assembly for Plant Systems highlights promoter-terminator interaction in gene regulation

Plant synthetic biology is a fast-evolving field that employs engineering principles to empower research and bioproduction in plant systems. Nevertheless, in the whole synthetic biology landscape, plant systems lag compared to microbial and mammalian systems. When it comes to multigene delivery to plants, the predictability of the outcome is decreased since it depends on three different chassis: E.coli, Agrobacterium, and the plant species. Here we aimed to develop standardised and streamlined tools for genetic engineering in plant synthetic biology. We have devised Mobius Assembly for Plant Systems (MAPS), a user-friendly Golden Gate Assembly system for fast and easy generation of complex DNA constructs. MAPS is based on a new group of small plant binary vectors (pMAPs) that contains an origin of replication from a cryptic plasmid of Paracoccuspantotrophus. The functionality of the pMAP vectors was confirmed by transforming the MM1 cell culture, demonstrating for the first time that plant transformation is dependent on the Agrobacterium strains and plasmids; plasmid stability was highly dependent on the plasmid and bacterial strain. We made a library of new short promoters and terminators and characterised them using a high-throughput protoplast expression assay. Our results underscored the strong influence of terminators in gene expression, and they altered the strength of promoters in some combinations and indicated the presence of synergistic interactions between promoters and terminators. Overall this work will further facilitate plant synthetic biology and contribute to improving its predictability, which is challenged by combinatorial interactions among the genetic parts, vectors, and chassis.

Scutellaria baicalensis – a small plant with large pro-health biological activities

Scutellaria baicalensis, known also as Huang-Qin is a traditional Chinese plant used in medicine for at least 2000 years. The plant is widely distributed in Japan, Korea, Mongolia and Russia, and is listed in Chinese Pharmacopoeia, European Pharmacopoeia and British Pharmacopoeia. The interest in Huang-Qin results from various biological activities which are primarily related to secondary plants metabolites consisting of flavonoids, phenolic compounds and terpenes. It is known that the compounds are active against numerous diseases and protect the organism against harmful pathogenic agents. Particular attention is paid to baicalein, wogonin and oroxylin A – which are characteristic secondary metabolites of the plant. In this paper, we focused on phytochemical analysis and selected biological activities used in periodontal and cardiovascular problems. The presented studies confirm the ability of Huang-Qin to scavenge free radicals, moreover, that it presents anti-bacterial, anti-inflammatory and enzyme inhibitory activities.

Mikroalglerin Antiviral Etkileri

Microalgae, also called phytoplankton by biologists, are very small plant-like organisms with a diameter of 1-50 micrometers without roots, stem and leaves. Microalgae, which have hundreds of thousands of species in both fresh waters and seas, form the lowest link of the food chain in aquatic ecosystems. Most species contain chlorophyll, use sunlight as an energy source, and convert carbon dioxide into biomass (biomass). Because of their role in the photosynthesis process, microalgae produce most of the oxygen in the atmosphere. It has a very wide biodiversity and is reported to contain more than 200 thousand species. As a result of genetic analysis, a continuous increase in microalgae species is observed. More than 15 thousand new chemical compounds have been discovered from algae in recent years. It has been observed that most of the bio compounds obtained from microalgae have antiviral effects. However, although extensive research has been done on the antibacterial, antioxidant and antifungal effects of these bioactive compounds, there is limited research on their antiviral effects. In these limited number of studies on the antiviral effects of microagines, it has been reported that some biocompounds isolated from algae may be effective against viruses that are the cause of diseases such as “HIV, SARS and AIDS”. However, the number of researches on viruses that cause today's biggest pandemic, such as coronavirus, of different biocompounds isolated from microalgae, is very small. To date, no vaccine that can be effective against the COVID-19 virus or a drug that can inhibit the reproduction of the virus has not been found. It is thought that micro or macro algae may be one of the most promising natural resources in solving this global health problem. Because Spirulina, which is a microalgae, has antiviral, anticancer, antidiabetic, antibiotic, antioxidant, prebiotic, cardiovascular system protective and antiallergic effects and these positive effects are caused by bioactive compounds found in high content (Rosales-Mendoza et al., 2020a). In this review, especially the antiviral effects of microalgae were tried to be summarized and it was tried to be emphasized that algae could be promising natural resources in the development of new antiviral drugs by our country's scientists.