Stable expression of Mycobacterium bovis antigen 85B in auxotrophic M. bovis bacillus Calmette-Guérin

ABSTRACT Interleukin 21 (IL-21) is a pleiotropic common cytokine receptor γ chain cytokine that promotes the effector functions of NK cells and CD8+ T cells and inhibits CD8+ T cell exhaustion during chronic infection. We found that the absolute number of short-lived effector CD8+ T cells (SLECs) (KLRG1high CD127low) decreased significantly in IL-21 receptor-deficient (IL-21R−/−) mice during Mycobacterium bovis bacillus Calmette-Guérin (BCG) infection. Early effector CD8+ T cells (EECs) (KLRG1low CD127low) were normally generated in IL-21R−/− mice after infection. Exhausted CD8+ T cells (PD-1high KLRG1low) were also normally generated in IL-21R−/− mice after infection. Mixed bone marrow (BM) chimera and transfer experiments showed that IL-21R on CD8+ T cells was essential for the proliferation of EECs, allowing them to differentiate into SLECs after BCG infection. On the other hand, the number of SLECs increased significantly after infection with recombinant BCG (rBCG) that secreted an antigen 85B (Ag85B)–IL-21 fusion protein (rBCG–Ag85B–IL-21), but the number of exhausted CD8+ T cells did not change after rBCG–Ag85B–IL-21 infection. These results suggest that IL-21 signaling drives the differentiation of SLECs from EECs but does not inhibit the exhaustion of CD8+ T cells following BCG infection in mice.

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Prosthetic joint infection with pseudo-tumoral aspect due to Mycobacterium bovis infection after Bacillus-Calmette-Guerin therapy

Annals of Physical and Rehabilitation Medicine ◽

10.1016/j.rehab.2017.08.001 ◽

2018 ◽

Vol 61 (1) ◽

pp. 62-64 ◽

Cited By ~ 4

Author(s):

B. Metayer ◽

P. Menu ◽

L. Khatchatourian ◽

P. Preuss ◽

M. Dauty ◽

...

Keyword(s):

Mycobacterium Bovis ◽

Prosthetic Joint Infection ◽

Joint Infection ◽

Bacillus Calmette Guerin ◽

Prosthetic Joint

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Mycobacterium bovis Bacillus Calmette-Guérin Killed by Extended Freeze-Drying Reduces Colitis in Mice

Gastroenterology ◽

10.1053/j.gastro.2011.05.002 ◽

2011 ◽

Vol 141 (2) ◽

pp. 642-652.e4 ◽

Cited By ~ 7

Author(s):

Micheline Lagranderie ◽

Christoph Kluge ◽

Helene Kiefer–Biasizzo ◽

Mohammad Abolhassani ◽

Marie–Anne Nahori ◽

...

Keyword(s):

Mycobacterium Bovis ◽

Freeze Drying ◽

Bacillus Calmette Guerin

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Impact of in vitro evolution on antigenic diversity of Mycobacterium bovis bacillus Calmette-Guerin (BCG)

Vaccine ◽

10.1016/j.vaccine.2014.07.113 ◽

2014 ◽

Vol 32 (45) ◽

pp. 5998-6004 ◽

Cited By ~ 42

Author(s):

Richard Copin ◽

Mireia Coscollá ◽

Efstratios Efstathiadis ◽

Sebastien Gagneux ◽

Joel D. Ernst

Keyword(s):

Mycobacterium Bovis ◽

Bacillus Calmette Guerin ◽

In Vitro Evolution ◽

Antigenic Diversity

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The double-sided effects of Mycobacterium Bovis bacillus Calmette–Guérin vaccine

npj Vaccines ◽

10.1038/s41541-020-00278-0 ◽

2021 ◽

Vol 6 (1) ◽

Author(s):

Junli Li ◽

Lingjun Zhan ◽

Chuan Qin

Keyword(s):

Literature Review ◽

Experimental Evidence ◽

Mycobacterium Bovis ◽

Virus Disease ◽

Bcg Vaccine ◽

Bacillus Calmette Guerin ◽

Mycobacterial Infections ◽

Specific Effects ◽

Mycobacterial Diseases ◽

Protection Mechanisms

AbstractBacillus Calmette–Guérin (BCG), the only vaccine proven to be effective against tuberculosis (TB), is the most commonly used vaccine globally. In addition to its effects on mycobacterial diseases, an increasing amount of epidemiological and experimental evidence accumulated since its introduction in 1921 has shown that BCG also exerts non-specific effects against a number of diseases, such as non-mycobacterial infections, allergies and certain malignancies. Recent Corona Virus Disease 2019 (COVID-19) outbreak has put BCG, a classic vaccine with significant non-specific protection, into the spotlight again. This literature review briefly covers the diverse facets of BCG vaccine, providing new perspectives in terms of specific and non-specific protection mechanisms of this old, multifaceted, and controversial vaccine.

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