scholarly journals Chemical sterilization of culture medium for in vitro multiplication of Cochlospermum regium

2018 ◽  
Vol 48 (9) ◽  
Author(s):  
Natália Helena Gavilan ◽  
Fernanda Cardoso Furlan ◽  
Alex Zichner Zorz ◽  
Leandro Silva de Oliveira ◽  
Wellington Ferreira Campos ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Culture Medium ◽  
Genetic Erosion ◽  
Chemical Agent ◽  
Shoot Induction ◽  
Popular Medicine ◽  
In Vitro Multiplication ◽  
Apical Buds ◽  
Chemical Sterilization

ABSTRACT: Cochlospermum regium roots are used in popular medicine and its extract has diverse phytochemical molecules some with antimicrobial activity, consequently exposing this specie to genetic erosion risks. Thus, the objective of this study was to develop an in vitro multiplication protocol using chemical sterilization of culture medium. Therefore, explants obtained from apical buds of C. regium seedlings were inoculated into with 0.05mg L-1 NAA and 1mg L-1 BAP sterilized by chemical agent sodium hypochlorite (NaOCl) at 0.001%, 0.003% and 0.005% of active chlorine (Cl). Autoclaved culture medium was used as control. Result showed that the contamination by bacterial at 91 days of cultivation was significantly (P<0.05) controlled by autoclaving, 0.001% and 0.005% Cl. Moreover, the callus induction in the culture medium with 0.001% and 0.005% Cl was, respectively, 30% and 20% major than autoclaving sterilization. There was not significant (P<0.05) in the percentage of shoot induction among the sterilization preparations methods, and 65% of the explants survived in the presence of culture medium with 0.005% Cl. Histological analyses indicated that the Cl did not have any deleterious effects on morphogenic events. These results indicated that the chemical sterilization using 0.001% - 0.005% Cl controlled the fungal and bacterial multiplication in the culture medium and no affected the C. regium explants development, becoming it an alternative to autoclaving method.

scholarly journals Optimization of explants in vitro sterilization protocol of some deciduous tree species

2021 ◽  
Vol 12 (3) ◽  
Author(s):  
O. Yu. Chornobrov ◽  
O. E. Tkachova
Keyword(s):  
Plant Material ◽  
Culture Medium ◽  
Deciduous Tree ◽  
Direct Regeneration ◽  
Planting Material ◽  
Number Of Factors ◽  
Tilia Platyphyllos ◽  
Chemical Sterilization ◽  
Sterilization Regime

One of the methods of obtaining planting material of deciduous plants, in particular common ash (Fraxinus excelsior L.), broad-leaved linden (Tilia platyphyllos Scop.) and silver birch (Betula pendula Roth) is microclonal propagation. Asepticity of explants is a prerequisite for microclonal plant propagation. Chemical sterilization with liquid substances is mostly used for this purpose. The mode of decontamination is influenced by a number of factors, in particular the genotype of the plant. The purpose of the study was to optimize the sterilization protocol of F. excelsior, T. platyphyllos and B. pendula explants for microclonal propagation. For research, 20–30 cm of shoots isolated from 12-year-old T. platyphyllos, 10-year-old B. pendula, and 15-year-old F. excelsior in February-March 2021 were used. Plant material was cultured according to conventional methods on a nutrient medium MS (Murashige & Skoog, 1962). Biotechnological methods were used (plant tissue culture in vitro, microclonal propagation). MS Excel software package was used to process the experimental data, the mean and its standard error were calculated. One-way analysis of variance (ANOVA) was performed to analyze the effect of explant sterilization on asepsis. The expediency of keeping plant material during the day in 0.1 % solution of «Samshit»  F. excelsior and 0.3 % solution «Fundazole»  T. platyphyllos and B. pendula is shown. The sterilization protocol of experimental plants (efficiency over 50 %) was optimized by using a stepwise method using 70 % ethyl alcohol, 1.0 % and 2.0 % AgNO3 and 2.5 % and 5.0 % NaClO. The effect of the sterilization regime of experimental plants on asepsis is statistically significant at the level of 5%. To initiate the explants, a culture medium according to the MS prescription was used with the addition of 0.25 mg/L kinetin and 2.0 g/L activated carbon. Further studies are aimed at developing a protocol for direct regeneration of microshoots of F. excelsior, T. platyphyllos and B. pendula under the action of components of the culture medium in vitro.

scholarly journals PENGARUH THIDIAZURON DAN HIDROLISAT KASEIN TERHADAP MULTIPLIKASI TUNAS SATOIMO (Colocasia esculenta (L.) Schott var antiquorum ) SECARA IN VITRO

2017 ◽  
Vol 4 (2) ◽  
pp. 17
Author(s):  
. Karyanti ◽  
Minda Kartini
Keyword(s):  
Culture Medium ◽  
Casein Hydrolysate ◽  
Shoot Multiplication ◽  
Colocasia Esculenta ◽  
Optimal Concentration ◽  
Plant Propagation ◽  
In Vitro Multiplication ◽  
Plant Seeds ◽  
Optimal Medium

Effect of Thidiazuron and Casein Hydrolysate on In Vitro Shoot Multiplication of Satoimo (Colocasia esculenta (L.) Schott var antiquorum)ABTRACTSatoimo (Colocasia esculenta (L.) Schott var antiquorum) is an alternative substitute of rice which has a big potential to be developed in Indonesia as an export commodity to Japan. Satoimo production needs to be increased to meet the demands for of the plant seeds. Plant propagation can be done using optimal media to stimulate the formation of shoots through, amongst others, the addition of thidiazuron (TDZ) and casein hydrolysate into the culture medium. This study aimed to determine the optimal concentration of TDZ and casein hydrolysate for in vitro multiplication of satoimo shoots. This research used RAL method with 2 factorials, namely the addition of TDZ at 0; 0.2; 0.6 mg/L concentrations, and of casein hydrolysate at 0; 150; 300; 450 mg/L concentrations. The results showed that the use of 0.6 mg/L TDZ and 150 mg/L casein hydrolysate resulted in the highest number of shoots, with the shoot average number of 6.9 per explant.Keywords: Casein hydrolysate, optimal medium, Satoimo, shoot multiplication, TDZ  ABTRAKSatoimo (Colocasia esculenta (L.) Schott var antiquorum) merupakan salah satu bahan alternatif pengganti beras yang memiliki peluang besar untuk dikembangkan di Indonesia, salah satunya sebagai komoditas ekspor ke negara Jepang. Produksi satoimo perlu ditingkatkan untuk memenuhi kebutuhan bibit tanaman tersebut. Perbanyakan tanaman dapat dilakukan menggunakan media yang optimal untuk merangsang pembentukan tunas, salah satunya dengan penambahan thidiazuron (TDZ) dan hidrolisat kasein pada media tanam. Penelitian ini bertujuan untuk mengetahui konsentrasi TDZ dan hidrolisat kasein yang optimal untuk perbanyakan tunas satoimo secara in vitro. Penelitian ini menggunakan metode RAL dengan 2 faktorial yaitu konsentrasi TDZ yang terdiri dari 0; 0,2; 0,6 mg/L dan konsentrasi hidrolisat kasein yang terdiri dari 0; 150; 300; 450 mg/L. Hasil penelitian menunjukkan bahwa pemberian TDZ 0,6 mg/L dan hidrolisat kasein 150 mg/L menghasilkan jumlah tunas tertinggi, dengan rata-rata tunas yang terbentuk 6,9 per eksplan.Kata kunci: Hidrolisat kasein, multiplikasi tunas, optimasi media, Satoimo, TDZ 

scholarly journals Propagation of Sedum spectabile Boreau in Leaf Culture in Vitro

2012 ◽  
Vol 40 (1) ◽  
pp. 107 ◽  
Author(s):  
Cuiqin YANG ◽  
Yaoguo QIN ◽  
Xin SUN ◽  
Shu YUAN ◽  
Honghui LIN
Keyword(s):  
Shoot Regeneration ◽  
Culture Medium ◽  
Shoot Proliferation ◽  
Shoot Formation ◽  
Root Induction ◽  
Ms Medium ◽  
Shoot Induction ◽  
Leaf Base ◽  
Stem Segments

An efficient protocol was established for Sedum spectabile Boreau propagation. Various leaf parts were used as explants to regenerate plantlets, the stem segments of which were cultured for shoot proliferation and plantlet multiplication. The results showed that the leaf base was the optimal explant, as compared to both the middle and the top of leaves, for shoot formation. The highest shoot induction of 88.9% was observed on MS medium supplemented with 0.6 mg/l TDZ and 0.1 mg/l NAA. Hyperhydric leaves obtained in primary culture developed first into abnormal somatic embryos 10 days after subculture, and then into hyperhydric plantlets after an additional 10 days. The hyperhydric plantlets reversed to normal plantlets when plant growth regulators were removed from culture medium. Further, stem segments from reversed plantlets were used for shoot regeneration and root induction. Optimal shoot regeneration was obtained in MS medium containing 0.6 mg/l TDZ with 0.1 mg/l NAA. Root induction and root mean number were all higher on auxin-free medium than on medium containing auxins.

scholarly journals IN VITRO SEED GERMINATION AND PLANT GROWTH OF “CABEÇA-DE-FRADE” (CACTACEAE)

2021 ◽  
Vol 34 (1) ◽  
pp. 1-8
Author(s):  
SHEILA VITÓRIA RESENDE ◽  
ALONE LIMA-BRITO ◽  
GABRIELA TORRES- SILVA ◽  
JOSÉ RANIERE FERREIRA DE SANTANA
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Culture Medium ◽  
Ornamental Plants ◽  
In Vitro Cultivation ◽  
Apical Segment ◽  
Aerial Portion ◽  
Low Concentrations ◽  
Chemical Sterilization

ABSTRACT The genus Melocactus (“cabeça-de-frade”) comprises 32 species in Brazil, of which M. glaucescens and M. paucispinus are threatened with extinction. The present work evaluated the effects of different concentrations of Murashige & Skoog (MS, MS/2 and MS/4) culture medium and sucrose (15 g L-1 and 30 g L-1) on in vitro seed germination and plant growth of M. glaucescens and the efficiency of sterilization with sodium hypochlorite (NaOCl) on in vitro seed germination and plant growth of M. glaucescens when using seeds and M. glaucescens and M. paucispinus when using apical segment of cladode. In M. glaucescens, the final germination at the different MS and sucrose concentrations varied between 53.5 and 68.1% and the best results for in vitro growth were observed with the lowest mineral salt (MS/2 and MS/4) and sucrose (15 g L-1) concentrations, with lengths of the aerial portion of 9.70 and 10.76 mm, respectively. There was no difference in seed germination and plant growth in chemical and autoclave medium. It is concluded that the use of chemical sterilization with NaOCl at low concentrations of salts (MS/2 and MS/4) and sucrose (15 g L-1) are quite advantageous for producing ornamental plants germinated in vitro and/or apical segment of cladode of M. glaucescens andM. paucispinus, representing a reduction of costs for in vitro cultivation of this species.

scholarly journals Antimicrobial Activity from Species Plectranthus amboinicus (Lour.) Spreng, a Review

2020 ◽  
pp. 1-14
Author(s):  
Juliane Maria dos Santos Silva ◽  
Jackson Roberto Guedes da Silva Almeida ◽  
Cristiane dos Santos Cerqueira Alves ◽  
Daniel Amando Nery ◽  
Livia Maria Oliveira Damasceno ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Therapeutic Potential ◽  
In Vivo Studies ◽  
Synergistic Activity ◽  
Popular Medicine ◽  
Plectranthus Amboinicus

Introduction: Nowadays, several bacteria have acquired resistance to available antimicrobial agents making necessary the search for new therapeutic alternatives. Plectranthus amboinicus L. is a succulent and aromatic herb, popularly known as thick leaf mint, used in popular medicine for the treatment of colds, digestive diseases, asthma, headache and to fight pathogenic bacteria activity. In view the antimicrobial activity of P. amboinicus this study had as aim to review publications involving researches about antimicrobial activity of this species. Materials and Methods: For this, PubMed, Scopus, Science Direct and Scielo databases were consulted in November 2020 using the keywords Plectranthus amboinicus and antimicrobial activity. In vitro and/or in vivo studies on the antimicrobial activity of the species in the last 10 years were considered. Results: The main microorganisms evaluated were: Klebsiella pneumoniae, Mycobacterium tuberculosis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and some Candida species. The essential oils had carvacrol, germacrene D, thymol and camphor as main constituents. Most studies evaluated the antimicrobial activity using broth dilution and agar diffusion methods. In most studies essential oil, extracts and/or isolated substances showed significant antimicrobial activity. Synergistic activity was also observed through association with antibiotics. Conclusion: P. amboinicus has therapeutic potential for antimicrobial treatments and can be an alternative to the treatment of resistant microorganisms and that further in vivo and clinical studies with the species are still needed.

scholarly journals Antimicrobial activity of calcium hydroxide associated with a new vehicle (Triethanolamine)

2016 ◽  
Vol 19 (1) ◽  
pp. 43
Author(s):  
Jaci Simi Junior ◽  
Ricardo Machado ◽  
Rafael Stiz ◽  
Cristiane Figueiredo ◽  
Luiz Pascoal Vansan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Growth Inhibition ◽  
Olive Oil ◽  
Calcium Hydroxide ◽  
Root Canal ◽  
Culture Medium ◽  
Significance Level ◽  
Petri Dishes ◽  
Antimicrobial Effectiveness

<p><strong>Objective:</strong> The aim of this study was to evaluate the antimicrobial effectiveness of calcium hydroxide associated with a two conventional (anesthetic liquid and olive oil) and a new vehicle (Triethanolamine). <strong>Material and methods:</strong> Initially, microorganisms were collected from four upper incisors presenting unsatisfactory root canal treatments and persistent apical periodontitis from the same patient using sterile absorbent paper cones placed inside the root canal following initial access and root filling removal. Next, they were grown in a BHI culture medium for 24 h. Afterwards, they were placed in 10 Petri dishes with 3 holes, where different formulations of the medications studied were placed. After 48 and 72 h, readings were taken of the growth inhibition halos. <strong>Results:</strong> After using Kruskal-Wallis test with significance level of 1% (α = 0.01) it was observed that, calcium hydroxide associated with Triethanolamine, produced results similar to those produced by the anesthetic liquid. Both combinations were superior to the association with olive oil. <strong>Conclusion:</strong> Considering the results of this <em>in vitro</em> study, it is possible to consider that Triethanolamine can be a viable alternative to be used as a vehicle associated with the calcium hydroxide.</p><p><strong>Keywords:</strong> Vehicles; Calcium hydroxide; Endodontics.</p>

scholarly journals Easy and efficient chemical sterilization of the culture medium for in vitro growth of gerbera using chlorine dioxide (ClO2)

2018 ◽  
Vol 24 (3) ◽  
pp. 218-224 ◽  
Author(s):  
Jean Carlos Cardoso ◽  
Ana Carolina Petit Inthurn
Keyword(s):  
Chlorine Dioxide ◽  
Aerial Part ◽  
Microbial Contamination ◽  
Culture Medium ◽  
In Vitro Cultivation ◽  
Fresh Weight ◽  
In Vitro Shoots ◽  
Chemical Sterilization ◽  
Number Of Leaves

Micropropagation techniques changed the production of clonal plantlets in the world. However, the high costs of micropropagated plantlets continue as the main constraint for the expansion of the technique. This paper aimed to test the use of the chemical sterilization of culture medium using chlorine dioxide (ClO2) for in vitro cultivation of gerbera. There was used gerbera in vitro shoots in the stage of rooting for these experiments, using 0.0035%, 0.0070% and 0.0105% of chlorine dioxide in the culture medium. Also, peracetic acid was tested previously for sterilization, but resulted in microbial contamination. Chemical sterilization of the culture medium was successfully using ClO2 at 0.0035% to 0.0105% (100% decontamination) at rooting and elongation stage of gerbera with production of plantlets with similar (number of leaves, total and root fresh weight) or higher quality (mainly aerial part) at rooting/elongation stage, compared with autoclaved culture medium. The increase of concentration of ClO2 also resulted in increasing of height and fresh weight of aerial part of gerberas. The ClO2 could replace the autoclaving with production of sterilized culture medium without phytotoxic problems to gerbera in vitro cultivation.

scholarly journals Ação do fluoreto de sódio sobre a formação de depósitos, “in vitro”, por bactérias placogênicas

2021 ◽  
pp. 35-57
Author(s):  
Eduardo Roberto Corrêa de Barros
Keyword(s):  
Stainless Steel ◽  
Dental Caries ◽  
Sodium Fluoride ◽  
Dental Plaque ◽  
Culture Medium ◽  
External Factor ◽  
Dry Weight ◽  
The Other ◽  
Chemical Agent

The author investigated the action of sodium fluoride at the concentration of 9,2% and 1 ppm of flúor (respectively used as mouthrinses and added to water consumed by population, in dental caries prevention) "in vitro", on dental plaque forming bacteria (Streptococcus mutans, samples AHT and IB). These concentrations were selected for the present study because its application does not require previous dental prophylaxis. Thus, any possible change in the deposit formation would be the result of the action caused by the chemical agent and not by any other external factor. The developed technique was based on the determination, by dry weight of the material placed over "STICKS" of stainless steel kept inside the culture medium, where the bacteria grew. The results did not show any statistically significant changes (at the Ievel of l%) when 1 ppm of fluor was added to the cultures. On the other hand, it was noticed a reduction statistically significant (at the level of 1%), in the deposit accumulated when it was added sodium fluoride at 0.2%.

scholarly journals Effect of different carbon sources on the in vitro multiplication of Annona sp.

2011 ◽  
Vol 35 (3) ◽  
pp. 487-493 ◽  
Author(s):  
José Raniere Ferreira de Santana ◽  
Renato Paiva ◽  
Ana Valéria de Souza ◽  
Lenaldo Muniz de Oliveira
Keyword(s):  
Culture Medium ◽  
Carbon Sources ◽  
Dry Weight ◽  
Nodal Segments ◽  
Seedling Production ◽  
In Vitro Multiplication ◽  
Bud Induction ◽  
Significant Difference ◽  
Annona Glabra

The Annonaceae family comprises approximately 2.300 species, some with significant commercial value. Although commercial plantations have suffered due to problems related to seedling production. As micropropagation is a viable technique for seedling production, the present work evaluated the effects of different carbon sources on in vitro bud induction in five Annonaceae species. Nodal segments obtained from plants of the Annona glabra, A. cauliflora, A. coriacea, A. bahiensis and Rollinia silvatica species were inoculated into solid WPM culture medium with 8.87 μM BAP, 0.86 mM of benomyl, and 87.64 mM of the following carbon sources: glucose, sucrose, fructose, galactose, sorbitol and maltose. We evaluated the buds number, the length and weight of the largest bud, the number of expanded leaves per bud, the length of the largest leaf and the dry matter of the buds. No significant difference was observed among the different carbon sources used in terms of the number of produced buds; however, the length of the largest bud, the number of expanded leaves, the length of the largest leaf, and dry weight of the buds presented significant difference according to the studied speciesas well as the carbon sources used, with the lowest value being obtained with sorbitol. The results obtained here indicated that, except for sorbitol, any of the carbohydrates tested could be used in the in vitro multiplication protocols for A. bahiensis, A. cauliflora, A. coriacea, A. glabra and R. silvatica.

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