Autologous platelet-rich plasma action on skin autografts in horses

ABSTRACT: This study evaluated the action of autologous platelet-rich plasma (PRP) on cutaneous wounds, containing skin autografts, in the gluteal region of horses. Seven healthy horses were used. Two 6 x 6cm cutaneous wounds were produced on each side of the gluteal region. Eight days after wound induction, grafts were performed with skin fragments harvested from the neck, as well as the application of PRP, prepared by double-centrifugation protocol. Wounds with autografts on the left side received PRP (group T), and those with autografts on the right side did not receive treatment (group C). Macroscopic and microscopic evaluations were performed, considering the integration of autografts and retraction of wound edges, as well as neovascularization, inflammatory infiltrate, young fibroblasts, collagenization, reepithelization and autografts integration. There was no difference between the groups (P > 0.05) in relation to most macroscopic and microscopic variables. However, neovascularization was significantly greater (p = 0.0191) in group T, on the 14th day after grafting. It is concluded that PRP favors the process of skin repair with autografts in horses, since it increases the neovascularization in the initial phase of wound healing. Furthermore, the PRP seems to positively influence the integration of the skin autografts and the retraction of the wound edges.

Download Full-text

Assessment of the healing of standardized wounds in rabbits treated serially with autologous platelet-rich plasma gel

Semina Ciências Agrárias ◽

10.5433/1679-0359.2016v37n6p4131 ◽

2016 ◽

Vol 37 (6) ◽

pp. 4131 ◽

Cited By ~ 2

Author(s):

Eliane Szücs dos Santos ◽

Cecília Braga Laposy ◽

Karina Gomes Barros Abegão ◽

Rosa Maria Barilli Nogueira ◽

Gisele Alborghetti Nai ◽

...

Keyword(s):

Therapeutic Potential ◽

Platelet Rich Plasma ◽

Cutaneous Lesions ◽

Cutaneous Wounds ◽

Morphometric Analyses ◽

Autologous Platelet Rich Plasma ◽

The Right ◽

Autologous Platelet

Recent studies have been carried out to evaluate the role of platelet-rich plasma (PRP) in the cicatrization of wounds; however, the protocols for treatment have been based on a single application of PRP.To evaluate the effect of autologous platelet-rich plasma in gel form on the cicatrization of cutaneous wounds in vivo experimental model, wounds were induced in the dorsal areas of six New Zealand white rabbits with the aid of an 8-mm punch. The right side was used as a control (A) and treated with 0.9% NaCl, whereas the left side (B) was treated serially with the autologous platelet-rich plasma gel. Lesions were assessed over a 17-day period. At days 0, 10 and 17, the animals were evaluated and morphological and morphometric analyses of the wounds were performed. At day 17, a biopsy was performed for histopathological evaluation. Macroscopically, wounds treated with PRP showed better cicatrization and higher contraction percentages than the control wounds. Regarding the percentage of wound contraction, it was found that the average treated wound with autologous platelet-rich plasma gelwas 95% while withthecontrolwas88%. We concluded that autologous platelet-rich plasma gel is effective and accelerates cicatrization when used serially in short intervals, thus confirming its therapeutic potential in cutaneous lesions and potential as an alternative wound treatment option.

Download Full-text

Ultrasound-guided injection of platelet-rich plasma in a patient with an ischeotibial muscle injury (Inyección guiada por ultrasonido de plasma rico en plaquetas en un paciente con lesión en el origen de la musculatura isquiotibial)

Retos ◽

10.47197/retos.v0i41.82520 ◽

2021 ◽

pp. 209-213

Author(s):

Lina Andrea Gomez ◽

Javier Fernando Bonilla Briceño ◽

Angélica Patricia Martinez Vasquez ◽

Ana Luisa Muñoz

Keyword(s):

Muscular Strength ◽

Platelet Rich Plasma ◽

Cost Effective ◽

Sports Activity ◽

Calidad De Vida ◽

Application Techniques ◽

Autologous Platelet Rich Plasma ◽

The Right ◽

Autologous Platelet

Platelet-rich plasma (PRP) is a platelet concentrate that is obtained after centrifugation and may influence tissues healing by growth factors released after platelet degranulation. This treatment has been used for the management of muscles, ligaments, and tendinopathy injuries with promising results, there is still limited clinical evidence for its use. In this study we present the report of a case of a 50-year-old soccer player who presented an injury at the origin of the ischeotibial muscles of the right thigh. On day 13, after the injury, autologous Platelet Rich Plasma (APRP) was applied under ultrasound (US) guidance. The control by US and Nuclear Magnetic Resonance (NMR) was performed two weeks after the PPR application, which show resolution of the hematoma, and tissue regeneration. The patient returned to his sports activity at week 12 after the PRP treatment, without pain and with improvement in muscular strength The PRP treatment could be an option to avoid surgical procedures in athletes because it could improve muscular strength and healing, added it is easy to obtain and apply. Further studies are necessary to reach a consensus regarding clinical indications, processing and PRP application techniques that rebound in alternatives to improve the quality of life of patients in a cost-effective way, as well as shortening the recovery time of athletes. Resumen. El plasma rico en plaquetas (PRP) es un concentrado de plaquetas que se obtiene después de centrifugación y puede influir en la curación de los tejidos por los factores de crecimiento liberados después de la desgranulación de las plaquetas. La terapia con PRP ha sido utilizada para el manejo de lesiones de tejidos blandos, como músculos, ligamentos y tendones con resultados prometedores. aunque la evidencia clínica actual para su uso es limitada. En este estudio presentamos el reporte del caso de un jugador de fútbol de 50 años que presentó lesión en el origen de la musculatura isquiotibial del muslo derecho. El día 13, después de la lesión, se le aplicó Plasma Rico en Plaquetas Autólogo (PRPA) guiado por ultrasonido (US). El control por US y resonancia magnética nuclear (RMN) se realizó dos semanas después de la aplicación del PRP, mostrando recuperación de la lesión y resolución del hematoma. El paciente volvió a realizar actividades deportivas en la semana 12 después del tratamiento, sin dolor y con mejoría de la fuerza muscular. El tratamiento con PRP podría ser una opción para evitar procedimientos quirúrgicos mejorando la fuerza muscular y la curación de la musculatura isqueotibial, adicionalmente el PRP es fácil de obtener y aplicar. De igual manera se necesitan más estudios para llegar a un consenso sobre las indicaciones clínicas, el procesamiento y las técnicas de aplicación de PRP que redunden en alternativas para mejorar la calidad de vida de los pacientes de una manera costo-efectiva, así como acortar el tiempo de recuperación de los atletas.

Download Full-text