scholarly journals Use of MALDI-TOF for identification and surveillance of gram-negative bacteria in captive wild psittacines

2022 ◽  
Vol 82 ◽  
Author(s):  
Y. M. Davies ◽  
L. S. Franco ◽  
F. B. Barbosa ◽  
C. L. Vanin ◽  
V. T. M. Gomes ◽  
...  
Keyword(s):  
Health Status ◽  
Accurate Method ◽  
Fecal Microbiota ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Proteus Hauseri ◽  
Tof Ms

Abstract Microbiological studies of the sanitary and health status of psittacine birds that will be reintroduced is important in evaluating whether these animals act as carriers of pathogenic agents to other animals and humans. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a faster and more accurate method to identify bacteria than conventional microbiology methods. The aim of this study was to evaluate the health status of psittacines housed in captivity, by assessment of Gram-negative bacteria from fecal microbiota through MALDI- TOF MS identification. The results indicate high frequency of Gram-negative bacteria in feces (96.5%), especially from the Enterobacteriaceae family (88.7%). The most prevalent bacteria were Escherichia coli (39.0%), Proteus vulgaris (12.2%), Klebsiella spp. (12.1%) and Raoultella ornithinolytica (8.7%). Proteus hauseri, Citrobacter spp., Morganella morgannii, Providencia rettgeri, Enterobacter spp. and Escherichia hermannii were isolated with lower frequency. . All these agents are potentially pathogenic for parrots and can cause systemic infections in other animals and humans. These findings reinforce that MALDI- TOF MS proved to be a rapid and accurate method of identification of the microorganism and evaluation of the health status of psittacines, providing relevant data to assist decision-making regarding the sanitary protocols in wildlife centers, and possible future reintroduction of wild birds.

scholarly journals Discrepancy in MALDI-TOF MS identification of uncommon Gram-negative bacteria from lower respiratory secretions in patients with cystic fibrosis

2015 ◽  
pp. 83 ◽  
Author(s):  
Atqah AbdulWahab ◽  
Saad Taj-Aldeen ◽  
Emad B. ibrahim ◽  
Eman Talaq ◽  
Marawan Abu-Madi ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Rapid Discrimination and Authentication of Korean Farmstead Mozzarella Cheese through MALDI-TOF and Multivariate Statistical Analysis

Metabolites ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 333
Author(s):  
Sujatha Kandasamy ◽  
Jayeon Yoo ◽  
Jeonghee Yun ◽  
Han-Byul Kang ◽  
Kuk-Hwan Seol ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Multivariate Statistical Analysis ◽  
Biomarker Discovery ◽  
Geographical Location ◽  
Accurate Method ◽  
Multivariate Statistical ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Geographical origin and authenticity are the two crucial factors that propel overall cheese perception in terms of quality and price; therefore, they are of great importance to consumers and commercial cheese producers. Herein, we demonstrate a rapid, accurate method for discrimination of domestic and import mozzarella cheeses in the Republic of Korea by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The protein profiles’ data aided by multivariate statistical analysis successfully differentiated farmstead and import mozzarella cheeses according to their geographical location of origin. A similar investigation within domestic samples (farmsteads/companies) also showed clear discrimination regarding the producer. Using the biomarker discovery tool, we identified seven distinct proteins, of which two (m/z 7407.8 and 11,416.6) were specific in farmstead cheeses, acting as potential markers to ensure authentication and traceability. The outcome of this study can be a good resource in building a database for Korean domestic cheeses. This study also emphasizes the combined utility of MALDI-TOF MS and multivariate analysis in preventing fraudulent practices, thereby ensuring market protection for Korean farmstead cheeses.

scholarly journals MASS-SPECTROMETRY IN MICROBIOLOGICAL PRACTICE OF SCIENTIFIC CENTRE OF OBSTETRICS, GYNECOLOGY AND PERINATOLOGY

2016 ◽  
pp. 52-58
Author(s):  
T. V. Priputnevich ◽  
A. R. Melkumyan ◽  
L. A. Lyubasovskaya ◽  
V. V. Muravieva ◽  
E. N. Ilina ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Species Identification ◽  
Gram Negative Bacteria ◽  
Analysis Method ◽  
Gram Negative ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Yeast Fungi ◽  
Tof Ms

Aim. Comparative evaluation of species identification of microorganisms by MALDI-TOF mass-spectrometry and automatic biochemical analyzer VITEK2 Compact30. Materials and methods. Species identification of18 400 isolates of microorganisms (staphylococci, streptococci, enterococci, enterobacteria, nonfermenting gram-negative bacteria, lactobacilli, anaerobes, yeast fungi, neisseriae), isolated from vagina of pregnant and non-pregnant women and from newborns, was carried out. Identification of the isolated microorganisms was carried out by automatic bac-teriologic analyzer VITEK2 Compact30 (BioMerieux, France) and MALDI-TOF-MS analysis method on AutoflexIII (Bruker Daltonics, Germany) mass-spectrometer. Results. Comparative identification of 2005 isolates of microorganisms was carried out. Sequencing of ribosomal RNA was used as a reference method. Authenticity of species identification my MALDI-TOF-MS analysis method was: for staphylococci (95.8%), enterococci (97.5%), enterobacteria (98.4%), nonfermenting gram-negative bacteria (93.6%), P-hemolytic staphylococci (93.8%), lactobacilli (92.8%), yeast fungi (99.9%). Conclusion. Introduction of MALDI-TOF-MS analysis technology into practical work of microbiological laboratories exceeds previously used methods of microbiological testing in terms of speed, cost and authenticity of identification of a wide spectrum of microorganisms.

scholarly journals MALDI-TOF MS for rapid detection and differentiation between Tet(X)-producers and non-Tet(X)-producing tetracycline-resistant Gram-negative bacteria

Virulence ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 77-88
Author(s):  
Zi-Jian Zheng ◽  
Ze-Hua Cui ◽  
Qiu-Yue Diao ◽  
Xin-Qing Ye ◽  
Zi-Xing Zhong ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Microarray-Based Nucleic Acid Assay and MALDI-TOF MS Analysis for the Detection of Gram-Negative Bacteria in Direct Blood Cultures

2018 ◽  
Vol 151 (2) ◽  
pp. 143-153
Author(s):  
Seon Young Kim ◽  
Jeong Su Park ◽  
Yun Ji Hong ◽  
Taek Soo Kim ◽  
Kiho Hong ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Blood Cultures ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Nucleic Acid Assay ◽  
Tof Ms

scholarly journals Direct Identification of Urinary Tract Pathogens from Urine Samples, Combining Urine Screening Methods and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

2016 ◽  
Vol 54 (4) ◽  
pp. 988-993 ◽  
Author(s):  
Melania Íñigo ◽  
Andreu Coello ◽  
Gema Fernández-Rivas ◽  
Belén Rivaya ◽  
Jessica Hidalgo ◽  
...  
Keyword(s):  
Laser Desorption ◽  
Urine Samples ◽  
Screening Methods ◽  
Gram Negative Bacteria ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms

Early diagnosis of urinary tract infections (UTIs) is essential to avoid inadequate or unnecessary empirical antibiotic therapy. Microbiological confirmation takes 24 to 48 h. The use of screening methods, such as cytometry and automated microscopic analysis of urine sediment, allows the rapid prediction of negative samples. In addition, matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) is a widely established technique in clinical microbiology laboratories used to identify microorganisms. We evaluated the ability of MALDI-TOF MS to identify microorganisms from direct urine samples and the predictive value of automated analyzers for the identification of microorganisms in urine by MALDI-TOF MS. A total of 451 urine samples from patients with suspected UTIs were first analyzed using the Sysmex UF-1000iflow cytometer, an automatic sediment analyzer with microscopy (SediMax), culture, and then processed by MALDI-TOF MS with a simple triple-centrifuged procedure to obtain a pellet that was washed and centrifuged and finally applied directly to the MALDI-TOF MS plate. The organisms in 336 samples were correctly identified, mainly those with Gram-negative bacteria (86.10%). No microorganisms were misidentified, and noCandidaspp. were correctly identified. Regarding the data from autoanalyzers, the best bacteriuria cutoffs were 1,000 and 200 U/μl for UF-1000iand SediMax, respectively. It was concluded that the combination of a urine screening method and MALDI-TOF MS provided a reliable identification from urine samples, especially in those containing Gram-negative bacteria.

Antibacterial activity of Lactobacilli detected in Algerian raw honeys against gram-negative bacteria

2019 ◽  
Vol 8 (3) ◽  
pp. 83-90
Author(s):  
Homrani Mounia ◽  
Dalache Fatiha ◽  
Bouzouina Mohammed ◽  
Nemmiche Said ◽  
Homrani AbdelKader
Keyword(s):  
Inhibition Zone ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Lactobacillus Pentosus ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Gram Staining ◽  
Lactobacillus Paraplantarum ◽  
Agar Well Diffusion Assay ◽  
Tof Ms

This study reports the isola on of Lactobacilli from Four (n=04) typical Apis Mellifeca raw honey samples collected from different regions in Algeria and the evalua on of their an bacterial ac vi es against Gram-negative bacteria. The isolation of Lactobacilli was performed using different media. Isolates were identified  firrstly by catalase test, Gram staining and cells morphology, and confirmed by MALDI-TOF-MS. An bacterial activities of Bacterial cultures of selected Lactobacilli and their cell free supernatants were tested by agar spot assays and agar well diffusion assay. A total of eighteen (n=18) bacteria isolated from honey samples were presump vely iden ed as Lac- tobacillus spp. based on their posi ve Gram reactions, absence of catalase and their rod shape. MALDI-TOF MS iden ca on confirmed that all isolates were classified as Lactobacillus genus, but the results for their species were inconclusive with uncertainty between, Lactobacillus pentosus, Lactobacillus plantarum and Lactobacillus paraplantarum. Isolates exhibited an microbial activity with inhibition zone diameters ranging from 11 ± 1.41 mm to 17.5 ± 0.71 mm. 6 on 11 Lactobacilli Supernatants demonstrated inhibitory ac vity against all target bacteria. This study reveals the existence of Lactobacilli in Algerian raw Honeys. These Lactobacilli possess an bacterial properties against Gram-negative bacteria, often responsible of human infections, and can be a favorable substitute to antibiotics.

Rapid susceptibility testing of multi-drug resistant Escherichia coli and Klebsiella by glucose metabolization monitoring

2019 ◽  
Vol 57 (8) ◽  
pp. 1271-1279 ◽  
Author(s):  
Maximilian Kittel ◽  
Peter Findeisen ◽  
Beniam Ghebremedhin ◽  
Thomas Miethke ◽  
Alexander Grundt ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Therapy ◽  
Susceptibility Testing ◽  
Gram Negative Bacteria ◽  
Drug Resistant ◽  
Gram Negative ◽  
Maldi Tof Ms ◽  
E Coli ◽  
Maldi Tof ◽  
Tof Ms

Abstract Background The increasing number of multi-drug resistant (MDR) bacteria provides enormous challenges for choosing an appropriate antibiotic therapy in the early phase of sepsis. While bacterial identification has been greatly accelerated by the introduction of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), the antibiotic susceptibility testing (AST) remains time-consuming. Here, we present a rapid susceptibility testing method for testing Gram-negative bacteria, exemplarily validated for Escherichia coli and Klebsiella spp. Methods Gram-negative isolates (E. coli and Klebsiella spp.) were either taken as single colonies from agar plates (n=136) or directly extracted and identified from positive blood cultures (n=42) using MALDI-TOF MS. Bacteria were incubated in glucose-supplemented Luria broths (LBs) each containing one antibiotic (ceftazidime, piperacillin, imipenem and ciprofloxacin), routinely used to classify Gram-negative bacteria in Germany. To determine susceptibility the dynamics of glucose utilization in bacterial suspensions were quantitatively measured in the presence or absence of antibiotics designated liquid-AST (L-AST). Results The L-AST can be run on clinical-chemistry analyzers and integrated into laboratory routines. It yields critical resistance information within 90–150 min downstream of a MS-based identification. The results showed a high concordance with routine susceptibility testing, with less than 1% very major errors (VME) and 3.51% major errors (ME) for 178 assessed isolates. Analysis of turnaround time (TAT) for 42 clinical samples indicated that L-AST results could be obtained 34 h earlier than the routine results. Conclusions As exemplified for E. coli and Klebsiella spp., L-AST provides substantial acceleration of susceptibility testing following MALDI-TOF MS identification. The assay is a simple and low-cost method that can be integrated into clinical laboratory to allow for 24/7 AST. This approach could improve antibiotic therapy.

scholarly journals Identification of the ‘Streptococcus anginosus group’ by matrix-assisted laser desorption ionization – time-of-flight mass spectrometry

2014 ◽  
Vol 63 (9) ◽  
pp. 1143-1147 ◽  
Author(s):  
Katherine Woods ◽  
David Beighton ◽  
John L. Klein
Keyword(s):  
Species Level ◽  
Direct Transfer ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Streptococcus Anginosus ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Transfer Method ◽  
Tof Ms

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) provides rapid, accurate and cost-effective identification of a range of bacteria and is rapidly changing the face of routine diagnostic microbiology. However, certain groups of bacteria, for example streptococci (in particular viridans or non-haemolytic streptococci), are less reliably identified by this method. We studied the performance of MALDI-TOF MS for identification of the ‘Streptococcus anginosus group’ (SAG) to species level. In total, 116 stored bacteraemia isolates identified by conventional methods as belonging to the SAG were analysed by MALDI-TOF MS. Partial 16S rRNA gene sequencing, supplemented with sialidase activity testing, was performed on all isolates to provide ‘gold standard’ identification against which to compare MALDI-TOF MS performance. Overall, 100 % of isolates were correctly identified to the genus level and 93.1 % to the species level by MALDI-TOF MS. However, only 77.6 % were correctly identified to the genus level and 59.5 % to the species level by a MALDI-TOF MS direct transfer method alone. Use of a rapid in situ extraction method significantly improved identification rates when compared with the direct transfer method (P<0.001). We recommend routine use of this method to reduce the number of time-consuming full extractions required for identification of this group of bacteria by MALDI-TOF MS in the routine diagnostic laboratory. Only 22 % (1/9) of Streptococcus intermedius isolates were reliably identified by MALDI-TOF MS to the species level, even after full extraction. MALDI-TOF MS reliably identifies S. anginosus and Streptococcus constellatus to the species level but does not reliably identify S. intermedius.

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