scholarly journals Apoptotic effect of Bulbine Natalensis and Chlorophytum Comosum in myelogenous Leukemia K562 cell line

2024 ◽  
Vol 84 ◽  
Author(s):  
B. Padayachee ◽  
F. Odun-Ayo ◽  
L. Reddy
Keyword(s):  
Cell Line ◽  
K562 Cell ◽  
Methanolic Extract ◽  
Membrane Integrity ◽  
Myelogenous Leukaemia ◽  
Myelogenous Leukemia ◽  
K562 Cell Line ◽  
Hematopoietic Stem ◽  
Anti Cancer ◽  
Apoptotic Effect

Abstract Bulbine natalensis and Chorophytum comosum are potential medicinal source for the treatment of cancers. Chronic myeloid leukaemia is a hematopoietic stem cells disorder treated by tyrosine kinase inhibitors but often cause recurrence of the leukaemia after cessation of therapy, hence require alternative treatment. This study determines the anti-cancer effect of leaf, root and bulb methanolic and aqueous extracts of B. natalensis and C. comosum in chronic human myelogenous leukaemia (K562) cell line by MTT, Hoechst bis-benzimide nuclear and annexin V stain assays. The root methanolic extract of B. natalensis and C. comosum showed a high cytotoxicity of 8.6% and 16.7% respectively on the K562 cell line at 1,000 μg/ml concentration. Morphological loss of cell membrane integrity causing degradation of the cell and fragmentation were observed in the root methanolic extract of both plants. A high apoptosis (p < 0.0001) was induced in the K562 cells by both leaf and root extracts of the C. comosum compared to the B. natalensis. This study shows both plants possess apoptotic effect against in vitro myelogenous leukaemia which contributes to the overall anti-cancer properties of B. natalensis and C. comosum to justify future therapeutic applications against chronic myelogenous leukaemia blood cancer.

scholarly journals Induction of erythropoietic colonies in a human chronic myelogenous leukemia cell line

Blood ◽  
1979 ◽  
Vol 54 (5) ◽  
pp. 1182-1187 ◽  
Author(s):  
R Hoffman ◽  
MJ Murnane ◽  
EJ Jr Benz ◽  
R Prohaska ◽  
V Floyd ◽  
...  
Keyword(s):  
Cell Line ◽  
Chronic Myelogenous Leukemia ◽  
Sodium Butyrate ◽  
K562 Cell ◽  
Leukemia Cell ◽  
Myelogenous Leukemia ◽  
Leukemia Cell Line ◽  
Erythroid Cell ◽  
K562 Cell Line ◽  
Chronic Myelogenous Leukemia Cell

The ability of cells derived from the K562 cell line to generate erythropoietic colonies was studied. The K562 cell line was derived from a patient with chronic myelogenous leukemia 8 yr ago by Lozzio and Lozzio. Rare benzidine-positive colonies formed when these cells were cloned in plasma clots (3 +/- 1/10(4) cells), and their number was not substantially increased by the addition of erythropoietin (9.5 +/- 1/10(4) cells). Sodium butyrate was capable of markedly enhancing the number of benzidine-positive colonies (19.5 +/- 1/10(4) cells) formed, while the combination of sodium butyrate plus erythropoietin exerted a synergistic effect on erythropoietic colony formation (57 +/- 4/10(4) cells). The K562 cell line is a long-term culture system that contains human erythropoietic stem cells. This cell line should be useful in future studies on the cellular and molecular events associated with human erythroid cell differentiation.

scholarly journals Induction of erythropoietic colonies in a human chronic myelogenous leukemia cell line

Blood ◽  
1979 ◽  
Vol 54 (5) ◽  
pp. 1182-1187 ◽  
Author(s):  
R Hoffman ◽  
MJ Murnane ◽  
EJ Jr Benz ◽  
R Prohaska ◽  
V Floyd ◽  
...  
Keyword(s):  
Cell Line ◽  
Chronic Myelogenous Leukemia ◽  
Sodium Butyrate ◽  
K562 Cell ◽  
Leukemia Cell ◽  
Myelogenous Leukemia ◽  
Leukemia Cell Line ◽  
Erythroid Cell ◽  
K562 Cell Line ◽  
Chronic Myelogenous Leukemia Cell

Abstract The ability of cells derived from the K562 cell line to generate erythropoietic colonies was studied. The K562 cell line was derived from a patient with chronic myelogenous leukemia 8 yr ago by Lozzio and Lozzio. Rare benzidine-positive colonies formed when these cells were cloned in plasma clots (3 +/- 1/10(4) cells), and their number was not substantially increased by the addition of erythropoietin (9.5 +/- 1/10(4) cells). Sodium butyrate was capable of markedly enhancing the number of benzidine-positive colonies (19.5 +/- 1/10(4) cells) formed, while the combination of sodium butyrate plus erythropoietin exerted a synergistic effect on erythropoietic colony formation (57 +/- 4/10(4) cells). The K562 cell line is a long-term culture system that contains human erythropoietic stem cells. This cell line should be useful in future studies on the cellular and molecular events associated with human erythroid cell differentiation.

The venom of the spider Macrothele raveni induces apoptosis in the myelogenous leukemia K562 cell line

2012 ◽  
Vol 36 (8) ◽  
pp. 1063-1066 ◽  
Author(s):  
Zhonghua Liu ◽  
Yan Zhao ◽  
Jing Li ◽  
Shiyan Xu ◽  
Changjun Liu ◽  
...  
Keyword(s):  
Cell Line ◽  
K562 Cell ◽  
Myelogenous Leukemia ◽  
K562 Cell Line ◽  
Leukemia K562 Cell

scholarly journals Autophagy is an important event for megakaryocytic differentiation of the chronic myelogenous leukemia K562 cell line

Autophagy ◽  
2009 ◽  
Vol 5 (8) ◽  
pp. 1092-1098 ◽  
Author(s):  
Pascal Colosetti ◽  
Alexandre Puissant ◽  
Guillaume Robert ◽  
Fréderic Luciano ◽  
Arnaud Jacquel ◽  
...  
Keyword(s):  
Cell Line ◽  
Chronic Myelogenous Leukemia ◽  
K562 Cell ◽  
Myelogenous Leukemia ◽  
K562 Cell Line ◽  
Megakaryocytic Differentiation ◽  
Leukemia K562 Cell

A New Series of Antileukemic Agents: Design, Synthesis, In Vitro and In Silico Evaluation of Thiazole-Based ABL1 Kinase Inhibitors

2020 ◽  
Vol 20 ◽  
Author(s):  
Ebru Zeytün ◽  
Mehlika D. Altıntop ◽  
Belgin Sever ◽  
Ahmet Özdemir ◽  
Doha E. Ellakwa ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Anticancer Activity ◽  
Cell Line ◽  
Antiproliferative Activity ◽  
In Silico ◽  
K562 Cell ◽  
Kinase Inhibitors ◽  
K562 Cell Line ◽  
Cytotoxic Effects ◽  
Atp Binding Site

Background: After the milestone approval of imatinib, more than 25 antitumor agents targeting kinases have been approved, and several promising candidates are in various stages of clinical evaluation. Objectives : Due to the importance of thiazole scaffold in targeted anticancer drug discovery, the goal of this work is the design of new thiazolyl hydrazones as potent ABL1 kinase inhibitors for the management of chronic myeloid leukemia (CML). Methods: New thiazolyl hydrazones (2a-p) were synthesized and investigated for their cytotoxic effects on K562 CML cell line. Compounds 2h, 2j and 2l showed potent anticancer activity against K562 cell line. The cytotoxic effects of these compounds on other leukemia (HL-60, MT-2 and Jurkat) and HeLa human cervical carcinoma cell lines were also investigated. Furthermore, their cytotoxic effects on mitogen-activated peripheral blood mononuclear cells (MA-PBMCs) were evaluated to determine their selectivity. Due to its selective and potent anticancer activity, compound 2j was benchmarked for its apoptosis-inducing potential on K562 cell line and inhibitory effects on eight different tyrosine kinases (TKs) including ABL1 kinase. In order to investigate the binding mode of compound 2j into the ATP binding site of ABL1 kinase (PDB: 1IEP), molecular docking study was conducted using MOE 2018.01 program. The QikProp module of Schrödinger’s Molecular modelling package was used to predict the pharmacokinetic properties of compounds 2a-p. Results: 4-(4-(Methylsulfonyl)phenyl)-2-[2-((1,3-benzodioxol-4-yl)methylene)hydrazinyl]thiazole (2j) showed antiproliferative activity against K562 cell line with an IC50 value of 8.87±1.93 µM similar to imatinib (IC50= 6.84±1.11 µM). Compound 2j was found to be more effective than imatinib on HL-60, Jurkat and MT-2 cells. Compound 2j also showed cytotoxic activity against HeLa cell line similar to imatinib. The higher selectivity index value of compound 2j than imatinib indicated that its antiproliferative activity was selective. Compound 2j also induced apoptosis in K562 cell line more than imatinib. Among eight TKs, compound 2j showed the strongest inhibitory activity against ABL1 kinase enzyme (IC50= 5.37±1.17 µM). According to molecular docking studies, compound 2j exhibited high affinity to the ATP binding site of ABL1 kinase forming significant intermolecular interactions. On the basis of in silico studies, this compound did not violate Lipinski's rule of five and Jorgensen's rule of three. Conclusion: Compound 2j stands out as a potential orally bioavailable ABL1 kinase inhibitor for the treatment of CML.

Optimization of differentiation condition for K562 cell line and rat erythroleukemia cell line

2018 ◽  
Vol 295 ◽  
pp. S251
Author(s):  
M. Otani ◽  
K. Iwashita ◽  
T. Utsumi ◽  
S. Kawamura
Keyword(s):  
Cell Line ◽  
K562 Cell ◽  
K562 Cell Line ◽  
Erythroleukemia Cell
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