scholarly journals Tracking a recent horizontal transfer event: The P-element reaches Brazilian populations of Drosophila simulans

2020 ◽  
Vol 43 (2) ◽  
Author(s):  
Ana M.L. Nascimento ◽  
Bráulio S.M.L. Silva ◽  
Marta Svartman ◽  
Gustavo C.S. Kuhn
Keyword(s):  
Horizontal Transfer ◽  
Drosophila Simulans ◽  
P Element ◽  
Transfer Event ◽  
Brazilian Populations

scholarly journals Evidence for horizontal transmission of the P transposable element between Drosophila species.

Genetics ◽  
1990 ◽  
Vol 124 (2) ◽  
pp. 339-355 ◽  
Author(s):  
S B Daniels ◽  
K R Peterson ◽  
L D Strausbaugh ◽  
M G Kidwell ◽  
A Chovnick
Keyword(s):  
Horizontal Transfer ◽  
Horizontal Transmission ◽  
Natural Populations ◽  
P Element ◽  
Base Substitution ◽  
Potential Donor ◽  
Transfer Event ◽  
P Elements ◽  
Autonomous Element ◽  
Group A

Abstract Several studies have suggested that P elements have rapidly spread through natural populations of Drosophila melanogaster within the last four decades. This observation, together with the observation that P elements are absent in the other species of the melanogaster subgroup, has lead to the suggestion that P elements may have entered the D. melanogaster genome by horizontal transmission from some more distantly related species. In an effort to identify the potential donor in the horizontal transfer event, we have undertaken an extensive survey of the genus Drosophila using Southern blot analysis. The results showed that P-homologous sequences are essentially confined to the subgenus Sophophora. The strongest P hybridization occurs in species from the closely related willistoni group. A wild-derived strain of D. willistoni was subsequently selected for a more comprehensive molecular examination. As part of the analysis, a complete P element was cloned and sequenced from this line. Its nucleotide sequence was found to be identical to the D. melanogaster canonical P, with the exception of a single base substitution at position 32. When the cloned element was injected into D. melanogaster embryos, it was able to both promote transposition of a coinjected marked transposon and induce singed-weak mutability, thus demonstrating its ability to function as an autonomous element. The results of this study suggest that D. willistoni may have served as the donor species in the horizontal transfer of P elements to D. melanogaster.

scholarly journals The recent invasion of natural Drosophila simulans populations by the P-element

2015 ◽  
Vol 112 (21) ◽  
pp. 6659-6663 ◽  
Author(s):  
Robert Kofler ◽  
Tom Hill ◽  
Viola Nolte ◽  
Andrea J. Betancourt ◽  
Christian Schlötterer
Keyword(s):  
Defense Mechanisms ◽  
Germ Line ◽  
Natural Populations ◽  
Genomic Data ◽  
Drosophila Simulans ◽  
P Element ◽  
Expression Data ◽  
Transfer Event ◽  
P Elements ◽  
Close Relatives

The P-element is one of the best understood eukaryotic transposable elements. It invaded Drosophila melanogaster populations within a few decades but was thought to be absent from close relatives, including Drosophila simulans. Five decades after the spread in D. melanogaster, we provide evidence that the P-element has also invaded D. simulans. P-elements in D. simulans appear to have been acquired recently from D. melanogaster probably via a single horizontal transfer event. Expression data indicate that the P-element is processed in the germ line of D. simulans, and genomic data show an enrichment of P-element insertions in putative origins of replication, similar to that seen in D. melanogaster. This ongoing spread of the P-element in natural populations provides a unique opportunity to understand the dynamics of transposable element spread and the associated piwi-interacting RNAs defense mechanisms.

scholarly journals Extensive Phenotypic Changes Associated with Large-scale Horizontal Gene Transfer

2013 ◽  
Author(s):  
Kevin Dougherty ◽  
Brian A Smith ◽  
Autum F Moore ◽  
Shannon Maitland ◽  
Chris Fanger ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Horizontal Gene Transfer ◽  
Horizontal Transfer ◽  
Large Scale ◽  
Pseudomonas Stutzeri ◽  
Transfer Event ◽  
Small Plasmid ◽  
Phenotypic Changes ◽  
Evolutionary Trajectories ◽  
Evolutionary Consequences

Horizontal gene transfer often leads to phenotypic changes within recipient organisms independent of any immediate evolutionary benefits. While secondary phenotypic effects of horizontal transfer (i.e. changes in growth rates) have been demonstrated and studied across a variety of systems using relatively small plasmid and phage, little is known about how size of the acquired region affects the magnitude or number of such costs. Here we describe an amazing breadth of phenotypic changes which occur after a large-scale horizontal transfer event (~1Mb megaplasmid) within Pseudomonas stutzeri including sensitization to various stresses as well as changes in bacterial behavior. These results highlight the power of horizontal transfer to shift pleiotropic relationships and cellular networks within bacterial genomes. They also provide an important context for how secondary effects of transfer can bias evolutionary trajectories and interactions between species. Lastly, these results and system provide a foundation to investigate evolutionary consequences in real time as newly acquired regions are ameliorated and integrated into new genomic contexts.

scholarly journals The P-element strikes again: the recent invasion of naturalDrosophila simulanspopulations

2015 ◽  
Author(s):  
Robert Kofler ◽  
Tom Hill ◽  
Viola Nolte ◽  
Andrea Betancourt ◽  
Christian Schlötterer
Keyword(s):  
Drosophila Melanogaster ◽  
Defense Mechanisms ◽  
Natural Populations ◽  
Genomic Data ◽  
P Element ◽  
Expression Data ◽  
Transfer Event ◽  
P Elements ◽  
Origins Of Replication ◽  
Close Relatives

The P-element is one of the best understood eukaryotic transposable elements. It invadedDrosophila melanogasterpopulations within a few decades, but was thought to be absent from close relatives, includingD. simulans. Five decades after the spread inD. melanogaster, we provide evidence that the P-element has also invadedD. simulans. P-elements inD. simulansappear to have been acquired recently fromD. melanogasterprobably via a single horizontal transfer event. Expression data indicate that the P-element is processed in the germline ofD. simulans, and genomic data show an enrichment of P-element insertions in putative origins of replication, similar to that seen inD. melanogaster. This ongoing spread of the P-element in natural populations provides an unique opportunity to understand the dynamics of transposable element spreads and the associated piRNA defense mechanisms.

Molecular analysis of P element behavior in Drosophila simulans transformants

1985 ◽  
Vol 200 (2) ◽  
pp. 258-265 ◽  
Author(s):  
Stephen B. Daniels ◽  
Linda D. Strausbaugh ◽  
Robert A. Armstrong
Keyword(s):  
Molecular Analysis ◽  
Drosophila Simulans ◽  
P Element

Comparative analysis of 1.688 satellite DNA on D. melanogaster species subgroup provides new insights of its pervasive presence throughout both chromatin domains and reveals a recent horizontal transfer event.

2018 ◽  
Author(s):  
Leonardo Gomes De Lima ◽  
Gustavo C. S. Kuhn
Keyword(s):  
Phylogenetic Analysis ◽  
Comparative Analysis ◽  
X Chromosome ◽  
Satellite Dna ◽  
Horizontal Transfer ◽  
Tandem Repeats ◽  
Specific Pattern ◽  
Chromosome X ◽  
Transfer Event ◽  
Species Specific

The 1.688 satellite DNA is present in the genome of Drosophila species from the melanogaster subgroup and has never been detected in species outside this subgroup. We investigated the presence and evolution of the 1.688 satDNA in all Drosophila genomes sequenced so far. Blast searches showed that 1.688 repeats are virtually confined to species from the melanogaster subgroup. Phylogenetic analysis of ~6,500 repeats extracted from D. melanogaster , D. simulans , D. sechellia , D. yakuba and D. erecta revealed the presence of 1.688 family on heterochromatin and euchromatin of all five species. Heterochromatic copies revealed a concerted mode of evolution and a species-specific pattern. Oppositely, euchromatic copies lack species-specific or array-specific pattern. Euchromatic arrays also showed a high number of insertions on 5Kb upstream/downstream of genes and in intronic regions. Unexpectedly, we found an array with at least three full 1.688 tandem repeats in the genome of D. willistoni . These repeats were highly similar to the ones present in the chromosome X of D. melanogaster , although both species have diverged from each other more than 35Mya, suggesting that 1.688 repeats from the X chromosome of D. melanogaster moved to D. willistoni by a recent horizontal transfer event.

scholarly journals Horizontal transfer of the n-TASE gene cluster in Burkholderia seminalis TC3.4.2R3 inferred from phylogenetic and molecular methods

2021 ◽  
Author(s):  
Sarina Tsui ◽  
Welington Luiz Araújo
Keyword(s):  
Comparative Genomics ◽  
Gene Transfer ◽  
Horizontal Gene Transfer ◽  
Horizontal Transfer ◽  
Horizontal Gene Transfer Event ◽  
Donor Strain ◽  
Biotechnological Applications ◽  
Transfer Event ◽  
Species Phylogeny ◽  
Homologous Genes

Abstract This study describes the n-TASE cluster in Burkholderia seminalis TC3.4.2R3, which was present in B. contaminans (CP046609.1), but absent in other related Burkholderia species. Phylogeny, comparative genomics and molecular analysis indicated it is not common to B. seminalis species, presenting similarity with homologous genes presents Aquamicrobium sp. SK-2 and B. contaminans LMG23361, probably acquired by an HGT (Horizontal Gene Transfer) event. It was not possible to determine which was the most likely donor strain of the n-TASE cluster. The HGT event did not occur in all strains of the Bcc group, nor in the B. seminalis, but it did occur punctually in the strain B. seminalis TC34.2R3. It has a correlation in biotechnological applications related processes. Aiming at understanding the involvement of the n-TASE cluster in the interaction of this bacterium in the environment, genes in this cluster will be inactivated, next.

scholarly journals Lipoxygenase in bacteria: a horizontal transfer event?

Microbiology ◽  
2001 ◽  
Vol 147 (12) ◽  
pp. 3199-3200 ◽  
Author(s):  
Helena Porta ◽  
Mario Rocha-Sosa
Keyword(s):  
Horizontal Transfer ◽  
Transfer Event

scholarly journals Mosaic Structure of the smpB-nrdEIntergenic Region of Salmonella enterica

1998 ◽  
Vol 180 (8) ◽  
pp. 2220-2223 ◽  
Author(s):  
Andreas J. Bäumler ◽  
Fred Heffron
Keyword(s):  
Escherichia Coli ◽  
Intergenic Region ◽  
Horizontal Transfer ◽  
Salmonella Enterica ◽  
Mosaic Structure ◽  
Transfer Event

ABSTRACT The Salmonella enterica smpB-nrdE intergenic region contains about 45 kb of DNA that is not present in Escherichia coli. This DNA region was not introduced by a single horizontal transfer event, but was generated by multiple insertions and/or deletions that gave rise to a mosaic structure in this area of the chromosome.

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