scholarly journals Molecular and serological detection of Leishmania spp. in horses from an endemic area for canine visceral leishmaniasis in southeastern Brazil

2018 ◽  
Vol 38 (6) ◽  
pp. 1058-1063 ◽  
Author(s):  
Julia C. Benassi ◽  
Graziella U. Benvenga ◽  
Helena L. Ferreira ◽  
Rodrigo M. Soares ◽  
Diogo T. Silva ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Endemic Area ◽  
Direct Sequencing ◽  
Antibody Test ◽  
Epidemiological Surveillance ◽  
Southeastern Brazil ◽  
Evolutionary Analysis ◽  
Leishmania Spp ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

ABSTRACT: This study aimed to verify the occurrence of Leishmania spp. and Leishmania (Leishmania) infantum in horses from a visceral leishmaniasis endemic area in Brazil. DNA samples from blood and conjunctival swab (CS) were tested by PCR and Indirect Immunofluorescence Antibody Test (IFAT). Although none of the horses was clinically sick, animals infected by Leishmania spp. were found and some could be characterized as infected by L. (L.) infantum. From 40 horses, 100% of the animals were positive by blood PCR, 90% (36/40) by CS PCR, and 2.5% (01/40) in serodiagnosis, by IFAT. Six from these 40 horses were L. (L.) infantum positive by blood PCR. Direct sequencing and analysis of amplicons resulted in a sequence to evolutionary analysis. Results indicate the presence of Leishmania spp. and L. (L.) infantum infecting healthy horses in Brazil. The presence of Leishmania spp. and L. (L.) infantum DNA in asymptomatic horses suggests that they can be important reservoirs of these parasites, a highly relevant finding for the epidemiological surveillance of the diseases they cause.

scholarly journals Toxoplasma gondii, Neospora caninum and Leishmania spp. serology and Leishmania spp. PCR in dogs from Pirassununga, SP

2015 ◽  
Vol 24 (4) ◽  
pp. 454-458 ◽  
Author(s):  
Nathália Mendonça de Seabra ◽  
Vanessa Figueredo Pereira ◽  
Marcos Vinícius Kuwassaki ◽  
Julia Cristina Benassi ◽  
Trícia Maria Ferreira de Sousa Oliveira
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Seropositivity Rate ◽  
Three Samples ◽  
Leishmania Spp ◽  
Polymerase Chain ◽  
The City ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

Abstract We examined the presence of antibodies against the parasites Toxoplasma gondii, Neospora caninum, and Leishmania spp., as well the presence of DNA from Leishmania spp., in dogs from Pirassununga - SP. The seropositivity rate was compared with the animals’ originating location. Three hundred seventy-three blood samples from the county’s kennel and local veterinary clinics were collected and analyzed. A total of 300 samples were tested for T. gondii and N. caninum using an indirect immunofluorescence antibody test (IFAT); 45% (135/300) were positive for T. gondii and 24.3% (73/300) were positive for N. caninum. Three hundred seventy-three samples were tested for Leishmania spp. using the IFAT. Of these, 4.6% (17/373) were positive. Additionally, 145 samples were tested using a polymerase chain reaction (PCR); of these samples, 0.7% (1/145) was positive. Considering the results, we conclude that these parasites are present in the city of Pirassununga - SP and that the animals have contact with the protozoan. It is therefore necessary to create methods for disease prevention to maintain both animal and human health in regard to leishmaniasis and toxoplasmosis.

scholarly journals PARTICIPATION OF TICKS IN THE INFECTIOUS CYCLE OF CANINE VISCERAL LEISHMANIASIS, IN TERESINA, PIAUÍ, BRAZIL

2014 ◽  
Vol 56 (4) ◽  
pp. 297-300 ◽  
Author(s):  
José Henrique Furtado Campos ◽  
Francisco Assis Lima Costa
Keyword(s):  
Visceral Leishmaniasis ◽  
Bone Marrow Aspiration ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Canine Visceral Leishmaniasis ◽  
Positive Skin ◽  
Leishmania Spp ◽  
The City ◽  
Infectious Cycle ◽  
Immunofluorescence Antibody Test

In this study, we detected Leishmania spp. infection in R. sanguineus collected from dogs that were naturally infected with L. (L.) infantum. We examined 35 dogs of both sexes and unknown ages. The infected dogs were serologically positive by the immunofluorescence antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA), and Quick Test-DPP (Dual Path Platform), as well as parasitological examination of a positive skin biopsy or sternal bone marrow aspiration. Ten negative dogs were included as controls. The ticks that infested these dogs were collected in pools of 10 adult females per animal. The PCR was performed with specific primers for Leishmania spp., which amplified a 720-bp fragment. Of the 35 analyzed samples, a product was observed in eight samples (8/35; 22.9%). We conclude that the presence of parasite DNA suggests that ticks participate in the zoonotic cycle of canine visceral leishmaniasis, in the city of Teresina, Piauí.

scholarly journals Leishmaniasis in dogs from recycling centers and from a neighborhood with adjacent forest in an urban area of Londrina, Paraná, Brazil

2016 ◽  
Vol 37 (3) ◽  
pp. 1407 ◽  
Author(s):  
Letícia Da Costa ◽  
Eloiza Teles Caldart ◽  
Bruno Bergamo Ruffolo ◽  
Roberta Dos Santos Toledo ◽  
Renata Cristina Ferreira Dias ◽  
...  
Keyword(s):  
Antibody Test ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Leishmania Spp ◽  
The Difference ◽  
Group 2 ◽  
Genus Leishmania ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody ◽  
Group 1

The leishmaniasis are a group of globally widespread parasitic diseases caused by a protozoa of the genus Leishmania, the state of Paraná accounts for 98% of leishmaniasis human cases in southern Brazil and the dogs might serve as a sentinel of the risk of infection to humans in a given area. The aim of this work was to study the epidemiology of leishmaniasis in dogs residents in two different environments, they are: recycling of solid waste centers (Group 1) and a neighborhood adjacent to a forest (Group 2), both in Londrina, Paraná State, Brazil and to determine epidemiological variables associated to infection in these different realities. Serum samples, randomly collected, from 61 dogs from Group 1 and from 74 dogs from Group 2 were submitted to Indirect Immunofluorescence Antibody Test (IFAT) to research for anti-Leishmania IgG antibodies. Of the 135 dogs analyzed, 13 animals (9.6%) were positive; nine (14.7%) in Group 1 and four (5.4%) in Group 2, the difference between the groups is not statistically significant. These results suggest the presence of the protozoan Leishmania spp. in both environments studied which indicate potential for infection in the human population.

scholarly journals Seroprevalence of Toxoplasma gondii in dogs of riverside communities of Mato Grosso Pantanal, Brazil

2016 ◽  
Vol 25 (4) ◽  
pp. 531-535 ◽  
Author(s):  
Juliana Yuki Rodrigues ◽  
Arleana do Bom Parto Ferreira de Almeida ◽  
Eveline da Cruz Boa Sorte ◽  
Naiani Domingos Gasparetto ◽  
Felipe Augusto Constantino Seabra da Cruz ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Antibody Test ◽  
Human Infection ◽  
Mato Grosso ◽  
Exact Test ◽  
Worldwide Distribution ◽  
Significant Difference ◽  
Chi Squared ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

Abstract Toxoplasma gondii is an intracellular protozoan with worldwide distribution and dogs act as sentinels of human infection. This search aimed to determine the occurrence of antibodies against T. gondii in dogs of the communities on the Cuiabá River, Mato Grosso and variables associated with infection. The dogs of the riverside communities in Cuiabá River, which includes Barranco Alto, Praia do Poço, Engenho Velho, Varginha, Bom Sucesso, Passagem da Conceição and São Gonçalo Beira Rio, were evaluated for the presence of T. gondii antibodies by indirect immunofluorescence antibody test (IFAT). The prevalence and factors associated with infection were calculated by chi-squared test (χ2) or Fisher’s exact test, and univariate and multiple analysis. Of the 248 dogs surveyed, 107 (43.1%) were seropositive for T. gondii. The seroprevalence ranged from 25.6% to 64.3%. There was no statistically significant difference between the communities studied (p > 0.05). As for the associated factors, the only statistically significant factor was that of dogs living with cats (p = 0.02), with approximately twice the risk of acquiring infection. In conclusion, the seroprevalence in dogs of riverside communities in the Baixada Cuiabana demonstrated that high rates of infection, being the factor associated with infection, contact with domestic cats.

scholarly journals Low prevalence of infection by Sarcocystis neurona in horses from the State of Alagoas, Brazil

2019 ◽  
Vol 28 (2) ◽  
pp. 298-302 ◽  
Author(s):  
Sandra Regina Fonseca de Araújo Valença ◽  
Müller Ribeiro-Andrade ◽  
Gastón Moré ◽  
Pedro Paulo Feitosa de Albuquerque ◽  
José Wilton Pinheiro Júnior ◽  
...  
Keyword(s):  
Risk Factors ◽  
Antibody Test ◽  
Sarcocystis Neurona ◽  
Animal Management ◽  
Serological Status ◽  
Potential Risk Factors ◽  
Low Exposure ◽  
Low Prevalence ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

Abstract The aim of this study was to determine the prevalence of infection by Sarcocystis neurona in horses and identify potential risk factors. Were analyzed 427 samples from 36 farms in 21 municipalities in the Alagoas State, Brazil. Presence of anti-S. neurona antibodies was diagnosed by indirect immunofluorescence antibody test (IFAT) and was confirmed using the immunoblot test. Risk factors were assessed through investigative questionnaires on animal management on the farms. The prevalence of anti-S.neurona antibodies was 2.8% (confidence interval, CI: 1.5-4.9%) from IFAT and 1.6% (CI:0.8-3.34%) from immunoblot, and there were positive horses on 16.6% of the studied farms. None of the variables studied presented associations with serological status for S. neurona. This is the first report on infection by S. neurona in horses reared in Alagoas, Brazil showing a low exposure to S. neurona in this region, but with significant numbers of foci.

scholarly journals Seroepidemiology ofLeishmaniaspp. in equids from Uberlândia, Minas Gerais, Brazil

2017 ◽  
Vol 47 (5) ◽  
Author(s):  
Patrícia Magalhães de Oliveira ◽  
Felipe Garcia ◽  
Fernanda Evers ◽  
Vinícius de Morais Barbosa ◽  
Diego Camilo Mora Obando ◽  
...  
Keyword(s):  
Economic Activity ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Epidemiological Survey ◽  
Minas Gerais ◽  
Southeastern Brazil ◽  
Wild Animals ◽  
Serum Samples ◽  
Leishmania Spp

ABSTRACT: This study evaluated the occurrence of Leishmania spp. in equids from Uberlândia, Minas Gerais, Southeastern Brazil. Serum samples from 257 healthy equids of Uberlândia were assessed for the presence of antibodies against Leishmania spp. by using the indirect fluorescent-antibody test. Additionally, an epidemiological survey was done to evaluate the possible risk factors (species of equids, age, gender, economic activity, and contact with domestic and wild animals) associated with the presence of antibodies against Leishmania spp. The total seroprevalence against Leishmania spp. was 24.1% (62/257); representing seroprevalence levels of 22.7% (55/242) in horses, 50% (4/8) in mules, and 42.9% (3/7) in ponies from all regions evaluated. The species of equid was the only factor that contributed to an elevated seroprevalence of Leishmania spp.

scholarly journals An indirect immunofluorescence antibody test employing whole eggs as the antigen for the diagnosis of abdominal angiostrongyliasis

2011 ◽  
Vol 106 (4) ◽  
pp. 390-393 ◽  
Author(s):  
Elizabeth Abrahams-Sandi ◽  
Paolo Mesén-Ramírez ◽  
Dimelsa Suarez-Chacón ◽  
Katherine Fernández-Quesada
Keyword(s):  
Indirect Immunofluorescence ◽  
Antibody Test ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

scholarly journals Giardia sp. and Cryptosporidium sp. in Iberian Wolf

2019 ◽  
Vol 70 (2) ◽  
pp. 1579
Author(s):  
A.L. PEREIRA ◽  
T. L. MATEUS ◽  
L. LLANEZ ◽  
S. D. DUARTE
Keyword(s):  
Canis Lupus ◽  
Antibody Test ◽  
Direct Immunofluorescence ◽  
Fecal Samples ◽  
First Report ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody ◽  
Canis Lupus Signatus ◽  
Iberian Wolf

A subsample consisting of fifty fecal samples from wild Iberian Wolf (Canis lupus signatus), from the northwest of Spain were collected in the field. The samples were analyzed for cysts of Giardia sp. and oocysts of Cryptosporidium sp. using a direct immunofluorescence antibody test (IFA). Giardia sp. and Cryptosporidium sp. were found in 20.0 % of the samples examined. Simple infections were more frequent (90.0 %) with seven (14.0 %) positive for Giardia sp. and two (4.0 %) positive for Cryptosporidium sp. To the authors’ knowledge, this is the first report of occurrence of Cryptosporidium sp. in Iberian Wolf.

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