scholarly journals Toxoplasma gondii, Neospora caninum and Leishmania spp. serology and Leishmania spp. PCR in dogs from Pirassununga, SP

2015 ◽  
Vol 24 (4) ◽  
pp. 454-458 ◽  
Author(s):  
Nathália Mendonça de Seabra ◽  
Vanessa Figueredo Pereira ◽  
Marcos Vinícius Kuwassaki ◽  
Julia Cristina Benassi ◽  
Trícia Maria Ferreira de Sousa Oliveira
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Seropositivity Rate ◽  
Three Samples ◽  
Leishmania Spp ◽  
Polymerase Chain ◽  
The City ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

Abstract We examined the presence of antibodies against the parasites Toxoplasma gondii, Neospora caninum, and Leishmania spp., as well the presence of DNA from Leishmania spp., in dogs from Pirassununga - SP. The seropositivity rate was compared with the animals’ originating location. Three hundred seventy-three blood samples from the county’s kennel and local veterinary clinics were collected and analyzed. A total of 300 samples were tested for T. gondii and N. caninum using an indirect immunofluorescence antibody test (IFAT); 45% (135/300) were positive for T. gondii and 24.3% (73/300) were positive for N. caninum. Three hundred seventy-three samples were tested for Leishmania spp. using the IFAT. Of these, 4.6% (17/373) were positive. Additionally, 145 samples were tested using a polymerase chain reaction (PCR); of these samples, 0.7% (1/145) was positive. Considering the results, we conclude that these parasites are present in the city of Pirassununga - SP and that the animals have contact with the protozoan. It is therefore necessary to create methods for disease prevention to maintain both animal and human health in regard to leishmaniasis and toxoplasmosis.

scholarly journals Occurrences of anti-Toxoplasma gondiiand anti-Neospora caninum antibodies in Barbary sheep at Curitiba zoo, southern Brazil

2014 ◽  
Vol 23 (2) ◽  
pp. 255-259 ◽  
Author(s):  
Vivien Midori Morikawa ◽  
Cristina Kraemer Zimpel ◽  
Igor Adolfo Dexheimer Paploski ◽  
Maria do Carmo Custódio de Souza Hunold Lara ◽  
Eliana Monteforte Cassaro Villalobos ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Southern Brazil ◽  
Intermediate Hosts ◽  
Blood Samples ◽  
Ammotragus Lervia ◽  
Barbary Sheep ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

Barbary sheep (Ammotragus lervia) have the potential to act as hosts of important infectious diseases, particularly zoonoses. Blood samples from 17 Barbary sheep at the Curitiba zoo were collected to evaluate occurrences of anti-Toxoplasma gondii and anti-Neospora caninum antibodies, tested using the indirect immunofluorescence antibody test (IFAT). Anti-T. gondii and anti-N. caninum antibodies were detected in 4/17 (23.5%) and 4/17 (23.5%) samples, respectively. The present study has shown that Barbary sheep at Curitiba zoo were exposed to T. gondii andN. caninum and therefore may act as intermediate hosts, spreading toxoplasmosis and neosporosis within and between species in shared areas.

scholarly journals Application of different techniques to detect Toxoplasma gondii in slaughtered sheep for human consumption

2015 ◽  
Vol 24 (4) ◽  
pp. 416-421 ◽  
Author(s):  
Annelise Castanha Barreto Tenório Nunes ◽  
Edna Maria Vieira da Silva ◽  
José Aelson de Oliveira ◽  
Elise Myuki Yamasaki ◽  
Pomy de Cássia Peixoto Kim ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Antibody Test ◽  
Human Consumption ◽  
Cerebral Tissue ◽  
Serum Samples ◽  
Kappa Test ◽  
Polymerase Chain ◽  
Immunofluorescence Antibody Test ◽  
Perivascular Cuff ◽  
Immunofluorescence Antibody

Abstract The aim of this study was to investigate occurrence of Toxoplasma gondii in sheep slaughtered in the state of Alagoas, Brazil, by means of different diagnosis techniques. Serum samples and tissues from 100 slaughtered sheep were used. To detect antibodies, the indirect immunofluorescence antibody test (IFAT) was used, and tissues from seropositive animals (cut-off ≥1:64) were submitted to Polymerase Chain Reaction (PCR) and immunohistochemistry (IHC). To assess the concordance between the direct techniques, the kappa test was used. In the IFAT, it was observed that 14% (14/100) of the ovine samples were serum-positive. In the PCR, 21.43% (3/14) of the animals were positive and in IHC, it was observed that 7.14% (1/14) were positively stained for T. gondii in cerebral tissue. Histopathologically, the predominant finding was the presence of mononuclear cell infiltrate in the heart and a perivascular cuff in the cerebrum and cerebellum. The concordance between the direct diagnosis techniques was moderate (k=0.44). Thus, it is important to use different direct techniques in diagnosing toxoplasmosis in naturally infected sheep.

scholarly journals Seroprevalence of Toxoplasma gondii in dogs of riverside communities of Mato Grosso Pantanal, Brazil

2016 ◽  
Vol 25 (4) ◽  
pp. 531-535 ◽  
Author(s):  
Juliana Yuki Rodrigues ◽  
Arleana do Bom Parto Ferreira de Almeida ◽  
Eveline da Cruz Boa Sorte ◽  
Naiani Domingos Gasparetto ◽  
Felipe Augusto Constantino Seabra da Cruz ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Antibody Test ◽  
Human Infection ◽  
Mato Grosso ◽  
Exact Test ◽  
Worldwide Distribution ◽  
Significant Difference ◽  
Chi Squared ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

Abstract Toxoplasma gondii is an intracellular protozoan with worldwide distribution and dogs act as sentinels of human infection. This search aimed to determine the occurrence of antibodies against T. gondii in dogs of the communities on the Cuiabá River, Mato Grosso and variables associated with infection. The dogs of the riverside communities in Cuiabá River, which includes Barranco Alto, Praia do Poço, Engenho Velho, Varginha, Bom Sucesso, Passagem da Conceição and São Gonçalo Beira Rio, were evaluated for the presence of T. gondii antibodies by indirect immunofluorescence antibody test (IFAT). The prevalence and factors associated with infection were calculated by chi-squared test (χ2) or Fisher’s exact test, and univariate and multiple analysis. Of the 248 dogs surveyed, 107 (43.1%) were seropositive for T. gondii. The seroprevalence ranged from 25.6% to 64.3%. There was no statistically significant difference between the communities studied (p > 0.05). As for the associated factors, the only statistically significant factor was that of dogs living with cats (p = 0.02), with approximately twice the risk of acquiring infection. In conclusion, the seroprevalence in dogs of riverside communities in the Baixada Cuiabana demonstrated that high rates of infection, being the factor associated with infection, contact with domestic cats.

scholarly journals Serosurvey for Leishmania spp., Toxoplasma gondii, Trypanosoma cruzi and Neospora caninum in neighborhood dogs in Curitiba-Paraná, Brazil

2016 ◽  
Vol 25 (4) ◽  
pp. 504-510 ◽  
Author(s):  
Caroline Constantino ◽  
Maysa Pellizzaro ◽  
Edson Ferraz Evaristo de Paula ◽  
Thállitha Samih Wischral Jayme Vieira ◽  
Ana Pérola Drulla Brandão ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Trypanosoma Cruzi ◽  
Urban Areas ◽  
Neospora Caninum ◽  
Antibody Test ◽  
Human Movement ◽  
Human Movements ◽  
Public Areas ◽  
Leishmania Spp ◽  
Protozoan Infections

Abstract Neighborhood dogs may act as reservoirs for several zoonotic protozoan infections, particularly in urban areas, thus constituting a potential public health threat. Accordingly, the aim of the present study was to evaluate the exposure of neighborhood dogs to four protozoan pathogens in public areas with high levels of human movement in Curitiba, southern Brazil. Blood samples from 26 neighborhood dogs were screened by means of the indirect immunofluorescent antibody test (IFAT) for Leishmania spp., Toxoplasma gondii, Trypanosoma cruzi and Neospora caninum, and a questionnaire was answered by the respective keeper. A total of 8/26 dogs (30.7%) seroreactive to T. gondii, 3/26 (11.5%) to N. caninum and 2/26 (7.7%) to both were identified. All the samples were seronegative for T. cruzi and Leishmania spp. Pathogen seroreactivity was not associated with the daily human movements or other epidemiological variables investigated (p > 0.05). In conclusion, the low seroprevalence for T. gondii and N. caninum indicated low environmental and food risk for animal infection and the seronegativity for Leishmania spp. and T. cruzi may reflect the absence of these pathogens in urban areas of Curitiba. Moreover, neighborhood dogs may be used as environmental sentinels for the presence of protozoan pathogens and their vectors.

scholarly journals PARTICIPATION OF TICKS IN THE INFECTIOUS CYCLE OF CANINE VISCERAL LEISHMANIASIS, IN TERESINA, PIAUÍ, BRAZIL

2014 ◽  
Vol 56 (4) ◽  
pp. 297-300 ◽  
Author(s):  
José Henrique Furtado Campos ◽  
Francisco Assis Lima Costa
Keyword(s):  
Visceral Leishmaniasis ◽  
Bone Marrow Aspiration ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Canine Visceral Leishmaniasis ◽  
Positive Skin ◽  
Leishmania Spp ◽  
The City ◽  
Infectious Cycle ◽  
Immunofluorescence Antibody Test

In this study, we detected Leishmania spp. infection in R. sanguineus collected from dogs that were naturally infected with L. (L.) infantum. We examined 35 dogs of both sexes and unknown ages. The infected dogs were serologically positive by the immunofluorescence antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA), and Quick Test-DPP (Dual Path Platform), as well as parasitological examination of a positive skin biopsy or sternal bone marrow aspiration. Ten negative dogs were included as controls. The ticks that infested these dogs were collected in pools of 10 adult females per animal. The PCR was performed with specific primers for Leishmania spp., which amplified a 720-bp fragment. Of the 35 analyzed samples, a product was observed in eight samples (8/35; 22.9%). We conclude that the presence of parasite DNA suggests that ticks participate in the zoonotic cycle of canine visceral leishmaniasis, in the city of Teresina, Piauí.

scholarly journals Molecular and serological detection of Leishmania spp. in horses from an endemic area for canine visceral leishmaniasis in southeastern Brazil

2018 ◽  
Vol 38 (6) ◽  
pp. 1058-1063 ◽  
Author(s):  
Julia C. Benassi ◽  
Graziella U. Benvenga ◽  
Helena L. Ferreira ◽  
Rodrigo M. Soares ◽  
Diogo T. Silva ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Endemic Area ◽  
Direct Sequencing ◽  
Antibody Test ◽  
Epidemiological Surveillance ◽  
Southeastern Brazil ◽  
Evolutionary Analysis ◽  
Leishmania Spp ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

ABSTRACT: This study aimed to verify the occurrence of Leishmania spp. and Leishmania (Leishmania) infantum in horses from a visceral leishmaniasis endemic area in Brazil. DNA samples from blood and conjunctival swab (CS) were tested by PCR and Indirect Immunofluorescence Antibody Test (IFAT). Although none of the horses was clinically sick, animals infected by Leishmania spp. were found and some could be characterized as infected by L. (L.) infantum. From 40 horses, 100% of the animals were positive by blood PCR, 90% (36/40) by CS PCR, and 2.5% (01/40) in serodiagnosis, by IFAT. Six from these 40 horses were L. (L.) infantum positive by blood PCR. Direct sequencing and analysis of amplicons resulted in a sequence to evolutionary analysis. Results indicate the presence of Leishmania spp. and L. (L.) infantum infecting healthy horses in Brazil. The presence of Leishmania spp. and L. (L.) infantum DNA in asymptomatic horses suggests that they can be important reservoirs of these parasites, a highly relevant finding for the epidemiological surveillance of the diseases they cause.

scholarly journals Detection and molecular analysis of Toxoplasma gondii and Neospora caninum from dogs with neurological disorders

2012 ◽  
Vol 45 (3) ◽  
pp. 365-368 ◽  
Author(s):  
Helio Langoni ◽  
Guilherme Matteucci ◽  
Bruno Medici ◽  
Lucilene Granuzio Camossi ◽  
Virgínia Bodelão Richini-Pereira ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Neurological Disorders ◽  
Neospora Caninum ◽  
Genotypic Variation ◽  
Antibody Test ◽  
Human Beings ◽  
Virulence Analysis ◽  
Polymerase Chain ◽  
Veterinary Hospital ◽  
Immunofluorescent Antibody Test

INTRODUCTION: Toxoplasma gondii and Neospora caninum are related Apicomplexa parasites responsible for systemic diseases in many species of animals, including dogs. METHODS: This study aimed to determine the occurrence of T. gondii and N. caninum infections in 50 dogs with neurological signs that were admitted to the Veterinary Hospital of Universidade Estadual Paulista, City of Botucatu, Brazil. All animals were screened for antibodies using an immunofluorescent antibody test for both parasites. Tissues of positive animals were bioassayed in mice (T. gondii) and gerbils (N. caninum), and DNA was analyzed using the polymerase chain reaction (PCR). Positive samples for T. gondii by PCR were typed using restriction fragment length polymorphism-PCR for 11 markers: SAG1, SAG2 (5′-3′-SAG2 and alt.SAG2), SAG3, Btub, GRA6, L358, c22-8, c29-6, PK1 and Apico, and CS3 marker for virulence analysis. RESULTS: Specific antibodies were detected in 11/50 (22%; 95% confidence interval (CI95%), 12.8-35.3%) animals for T. gondii and 7/50 (14%; CI95%, 7.02-26.3%) for N. caninum. In the bioassay and PCR, 7/11 (63.6%; CI95%, 34.9-84.8%) samples were positive for T. gondii and 3/7 (42.9%; CI95%I, 15.7-75.5%) samples were positive for N. caninum. Three different genotypes were identified, but only 1 was unique. CONCLUSIONS: These data confirm the presence of T. gondii and N. caninum in dogs from Brazil, indicating the importance of this host as a sentinel of T. gondii for human beings, and the genotypic variation of this parasite in Brazil.

scholarly journals Antigenic and genetic characterization of the first rabies virus isolated from the bat Eumops perotis in Brazil

2008 ◽  
Vol 50 (2) ◽  
pp. 95-99 ◽  
Author(s):  
Juliana Galera Castilho ◽  
Flávia Marchizeli Canello ◽  
Karin Corrêa Scheffer ◽  
Samira Maria Achkar ◽  
Maria Luiza Carrieri ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Antibody Test ◽  
Nucleoprotein Gene ◽  
Adult Mice ◽  
Vampire Bats ◽  
The City ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody

Although the main transmitters of rabies in Brazil are dogs and vampire bats, the role of other species such as insectivorous and frugivorous bats deserves special attention, as the rabies virus has been isolated from 36 bat species. This study describes the first isolation of the rabies virus from the insectivorous bat Eumops perotis. The infected animal was found in the city of Ribeirão Preto, São Paulo. The virus was identified by immunofluorescence antibody test (FAT) in central nervous system (CNS) samples, and the isolation was carried out in N2A cell culture and adult mice. The sample was submitted to antigenic typing using a panel of monoclonal antibodies (CDC/Atlanta/USA). The DNA sequence of the nucleoprotein gene located between nucleotides 102 and 1385 was aligned with homologous sequences from GenBank using the CLUSTAL/W method, and the alignment was used to build a neighbor-joining distance-based phylogenetic tree with the K-2-P model. CNS was negative by FAT, and only one mouse died after inoculation with a suspension from the bat's CNS. Antigenic typing gave a result that was not compatible with the patterns defined by the panel. Phylogenetic analysis showed that the virus isolated segregated into the same cluster related to other viruses isolated from insectivorous bats belonging to genus Nyctinomops ssp. (98.8% nucleotide identity with each other).

scholarly journals Leishmaniasis in dogs from recycling centers and from a neighborhood with adjacent forest in an urban area of Londrina, Paraná, Brazil

2016 ◽  
Vol 37 (3) ◽  
pp. 1407 ◽  
Author(s):  
Letícia Da Costa ◽  
Eloiza Teles Caldart ◽  
Bruno Bergamo Ruffolo ◽  
Roberta Dos Santos Toledo ◽  
Renata Cristina Ferreira Dias ◽  
...  
Keyword(s):  
Antibody Test ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Leishmania Spp ◽  
The Difference ◽  
Group 2 ◽  
Genus Leishmania ◽  
Immunofluorescence Antibody Test ◽  
Immunofluorescence Antibody ◽  
Group 1

The leishmaniasis are a group of globally widespread parasitic diseases caused by a protozoa of the genus Leishmania, the state of Paraná accounts for 98% of leishmaniasis human cases in southern Brazil and the dogs might serve as a sentinel of the risk of infection to humans in a given area. The aim of this work was to study the epidemiology of leishmaniasis in dogs residents in two different environments, they are: recycling of solid waste centers (Group 1) and a neighborhood adjacent to a forest (Group 2), both in Londrina, Paraná State, Brazil and to determine epidemiological variables associated to infection in these different realities. Serum samples, randomly collected, from 61 dogs from Group 1 and from 74 dogs from Group 2 were submitted to Indirect Immunofluorescence Antibody Test (IFAT) to research for anti-Leishmania IgG antibodies. Of the 135 dogs analyzed, 13 animals (9.6%) were positive; nine (14.7%) in Group 1 and four (5.4%) in Group 2, the difference between the groups is not statistically significant. These results suggest the presence of the protozoan Leishmania spp. in both environments studied which indicate potential for infection in the human population.

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