Effects of Lysophospholipid and Lipase Enzyme Supplementation to Low Metabolizable Energy Diets on Growth Performance, Intestinal Morphology and Microbial Population and Some Blood Metabolites in Broiler Chickens

Abstract Two experiments were conducted to investigate the effects of a combined α-galactosidase and xylanase preparation on nutrients digestibility and growth performance in broiler chickens. Experiment 1 had 240 broilers allocated to 3 treatments with the dietary supplementation of 0, 300 and 500 g/t of the enzyme combination. Diet and amino acid (AA) digestibility were assessed. Experiment 2 was a 2 × 3 (enzyme × diet) factorial arrangement with 10 replicates of 12 male broilers per replicate. Diets were based on corn-Soybean meal (SBM) diet and had 3 nutritional levels (normal, 2% apparent metabolizable energy (AME) and crude protein (CP) reduction, and 4% AME and CP reduction). Each of these diets was fed with or without enzyme supplementation. Growth performance, chyme viscosity, nutrients digestibility, and endogenous enzymes activity were assessed. In experiment 1, enzyme supplementation improved the digestibility of Ca (P = 0.025) and ileal digestibility of total AA, Pro, Alu, Ile, Lys, His, Thr, Glu, Val, Leu, Tyr and Phe (P < 0.05), and also tended to increase the AME of diets (P < 0.10). In experiment 2, broilers fed the corn-SBM diet with 4% nutrient reduction had better growth performance (P < 0.05), jejunal digesta viscosity at 42 days (P < 0.01), and lower digestibility of gross energy (GE) (P < 0.05) when compared to those fed the normal nutrient diet. Enzyme inclusion increased digestibility of CP (P = 0.044), GE (P = 0.009), raffinose (P < 0.001) and stachyose (P < 0.001), improved average daily gain (P = 0.031), and reduced jejunal digesta viscosity at 42 days (P = 0.011). Besides, similar improvements trend in amylase, trypsin, sucrase, and maltase activity with enzyme inclusion were observed as with energy. These data support that the enzyme supplementation increased nutrients and ileal amino acid digestibility, improved performance and endogenous enzymes activity.

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Effects of flavomycin and probiotic supplementation to diets containing different sources of fat on growth performance, intestinal morphology, apparent metabolizable energy, and fat digestibility in broiler chickens

Poultry Science ◽

10.3382/ps.2011-01844 ◽

2012 ◽

Vol 91 (4) ◽

pp. 918-927 ◽

Cited By ~ 20

Author(s):

S.D. Sharifi ◽

A. Dibamehr ◽

H. Lotfollahian ◽

B. Baurhoo

Keyword(s):

Growth Performance ◽

Broiler Chickens ◽

Metabolizable Energy ◽

Intestinal Morphology ◽

Probiotic Supplementation ◽

Apparent Metabolizable Energy ◽

Different Sources ◽

Fat Digestibility

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Impact of exogenous carbohydrases and phytase on growth performance and nutrient digestibility in broilers

Canadian Journal of Animal Science ◽

10.4141/cjas2012-138 ◽

2013 ◽

Vol 93 (2) ◽

pp. 243-249 ◽

Cited By ~ 8

Author(s):

H. Lu ◽

S.A. Adedokun ◽

A. Preynat ◽

V. Legrand-Defretin ◽

P. A. Geraert ◽

...

Keyword(s):

Body Weight ◽

Growth Performance ◽

Broiler Chickens ◽

Body Weight Gain ◽

Negative Control ◽

Nutrient Digestibility ◽

Metabolizable Energy ◽

Feed Ratio ◽

Exogenous Enzyme ◽

Enzyme Supplementation

Lu, H., Adedokun, S. A., Preynat, A., Legrand-Defretin, V., Geraert, P. A., Adeola, O. and Ajuwon, K. M. 2013. Impact of exogenous carbohydrases and phytase on growth performance and nutrient digestibility in broilers. Can. J. Anim. Sci. 93: 243–249. The effect of supplementing corn–soybean-based diets with Rovabio®Max AP, an exogenous enzyme mix with carbohydrase (xylanase and β-glucanase) and phytase activities to broiler chickens was evaluated. Male Ross 708 broilers were assigned to four diets: positive control (PC), negative control (NC), deficient in metabolizable energy (ME), crude protein (CP), calcium (Ca) and non-phytate phosphorus (nPP), relative to the PC, by 100 kcal kg−1, 5 g kg−1, 1.1 g kg−1and 1.6 g kg−1, respectively, in the starter and grower diets; and NC+Rovabio®at 0.05 or 0.075 g kg−1diet. Starter diets were fed from day 0 to day 21 and grower diets from day 22 to day 42. Body weight (BW) and body weight gain on days 22 and 42 were increased (P<0.05) by enzyme supplementation. Enzyme addition increased (P<0.05) feed intake from day 22 to day 42 and overall (day 0 to day 42). Gain to feed ratio (G:F) was significantly increased (P<0.05) with enzyme supplementation during both starter and grower phases. Enzyme addition increased (P<0.05) serum phosphorus (P) concentration on day 21. On day 42 enzyme supplementation increased (P<0.05) the apparent ileal digestibility of P, dry matter (DM), energy, and N. Addition of a combination of carbohydrases (xylanase and β-glucanase) and phytase to corn–soybean-based diets deficient in ME, CP, Ca and P resulted in a significant increase in growth performance and utilization of P, DM, energy and N in broiler chickens.

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Growth performance, internal organ traits, intestinal morphology, and microbial population of broiler chickens fed quinoa seed–based diets with phytase or protease supplements and their combination

Tropical Animal Health and Production ◽

10.1007/s11250-021-02980-0 ◽

2021 ◽

Vol 53 (6) ◽

Author(s):

Mohammad Yusef Asnaashari Amiri ◽

Mohammad Ali Jafari ◽

Mehrdad Irani

Keyword(s):

Growth Performance ◽

Broiler Chickens ◽

Microbial Population ◽

Intestinal Morphology

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In ovo Administration of Nucleosides Improved the Performance, Apparent Metabolizable Energy and Gut Development in Broiler Chickens

Frontiers in Veterinary Science ◽

10.3389/fvets.2020.583748 ◽

2020 ◽

Vol 7 ◽

Author(s):

Marappan Gopi ◽

Villavan Manojkumar ◽

Ashok Kumar Verma ◽

Putan Singh ◽

Jaydip Jaywant Rokade ◽

...

Keyword(s):

Growth Performance ◽

Plasma Protein ◽

Broiler Chickens ◽

Metabolizable Energy ◽

Intestinal Morphology ◽

Gut Development ◽

In Ovo ◽

Plasma Protein Concentration ◽

Apparent Metabolizable Energy ◽

Phosphate Buffered Saline

An in ovo study on the effect of the administration of a combination of nucleosides (25, 50, and 100 mg/egg) on hatchability, growth performance, energy metabolizability, and intestinal morphology in broilers was carried out. Four hundred eighty (480) fertile eggs were divided into four groups (in four replicates each having 30 eggs). On the 18th days of incubation of the eggs, candling was carried out and the fertile eggs were selected and given one of the four in ovo administrations. Group one served as control and was injected with phosphate-buffered saline (PBS). The other groups were given in ovo administration of nucleosides (25, 50, and 100 mg/egg) at 100 μl through the yolk sac route, and chicks of respective groups were hatched out. Among the experimental groups, the hatchability was comparable; however, the hatchability was affected in the group injected with a higher level of nucleosides at 100 mg/egg. The hatched out chicks from higher doses of nucleosides (50 and 100 mg) had higher body weight (BW) (P < 0.05) than the control. Higher energy metabolizability (%) was observed in nucleoside-injected groups. Plasma protein concentration was higher in groups administered with nucleosides (50 and 100 mg). Histologically, the intestinal villi length was maximum in 100 mg-injected group followed by 50 and 25 mg. Relative expression of homeobox (Cdx) in the jejunum was significantly (P < 0.05) upregulated in all the injected groups at 3, 7, and 14 days of age. Nucleoside-administered groups had better performance, energy metabolizability, and intestinal morphology. Among the experimental groups, the administration of nucleosides at 50 mg/egg resulted in higher growth performance, plasma protein, intestinal surface, and villi development in broiler chickens.

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