Kecernaan Nutrien dan Status Fisiologis Domba Akhir Kebuntingan dengan Ransum Flushing Minyak Sawit dan Minyak Lemuru

The objective of this study was to evaluate nutrient digestibility and physiological status of local sheep at late pregnancy fed with flushing diet containing palm oil, lemuru oil, and their combination. In this research, the livestock used were 12 female local sheep at late pregnancy with an average body weight of 43.58 ± 5.57 kg were placed in metabolic cages. This study used a randomized block design (RBD) with 4 treatments and 3 block replications. The treatments were P1 = control ration (30% grass + 70% concentrate), P2 = control ration + 6% palm oil supplementation, P3 = control ration + 3% lemuru oil and 3% palm oil supplementation, P4 = control ration + 6% lemuru oil supplementation. The feed consumption, nutrient digestibility, and physiological status were measured in this study. The data obtained were analyzed using ANOVA. Means values differences were analyzed using Duncan test. The results showed that the addition of palm oil and lemuru oil to the concentrate diet increased (p<0.05) crude fat consumption, dry matter digestibility, and crude fat digestibility, but decreased (p<0.05) Nitrogen free extract consumption. Addition of palm oil and lemuru oil on the concentrate diet had no significant effect on dry matter consumption, crude protein consumption, crude fiber consumption, crude protein digestibility, crude fiber digestibility, Nitrogen free extract digestibility, and TDN value. The treatments did not affect significantly on physiological status. It can be concluded that the use of 6% palm oil in the ration can increase crude fat consumption and crude fat digestibility in pregnant ewes. Key words: digestibility, ewes, flushing, oil, physiological status

Effects of Garlic Allium sativum Powder on Nutrient Digestibility, Haematology, and Immune and Stress Responses in Eurasian Perch Perca fluviatilis Juveniles

The supplementation of fish diets with phytogenics can increase growth performance and can modulate immune system response. European perch Perca fluviatilis (initial weight 25.0 ± 0.4 g) were fed a diet including 0 (Control), 10 (G10), 20 (G20), and 30 (G30) g kg−1 garlic powder. No significant difference in the growth parameters and somatic indices were observed. Significantly higher fat digestibility was observed in G10 and G30 diets compared to in the control and G20 diets(p < 0.05). Significantly greater red blood cell and white blood cell counts were observed with the G10 diet (p < 0.05). Garlic significantly decreased serum cholesterol in all of the experimental groups. Serum albumin was significantly higher in the G10 and G20 diets (p < 0.05). Immediately after the overcrowding stress challenge, the garlic groups showed significantly higher cortisol levels than the control group, while no significant difference was observed in the glucose concentration among groups. At 1 h post-stress, all of the groups that had been fed a garlic-supplemented diet showed lower cortisol levels than the control group, and this trend was maintained at 6 and 24 h post stress (p < 0.05), and glucose level in all garlic groups was significantly lower than control (p < 0.05). Garlic at 10 g kg−1 in feed can improve apparent fat digestibility and selected blood parameters and can enhance resistance against high-density and net handling stress in Eurasian perch.

The effect of different medium chain fatty acids, calcium butyrate, and salinomycin on performance, nutrient utilization and gastrointestinal tract of chicken of Polish Green Legged Partridge hen

The present study aimed to determine the effect of diets supplemented by feed additives, on the growth performance and digestive tract functioning in chicken of green-legged partridge hen (GLPH). Two types of diets were used. The both “low-digestible” (LD) diet characterized by high level of crude fibre and lard, and “high-digestible” (HD) diet characterized by low content of crude fibre and soybean oil were supplemented by different additives such as 0,34% DM (dry matter) salinomycin, 0,30 % DM medium-chain triglycerides (MCT), 1,00 % DM calcium butyrate (CB), or 0,85 % DM medium-chain fatty acids (MCFA). The experiment was conducted on 960 one-day-old male chickens of GLPH, randomly divided into 10 treatment groups. One group consisted of 12 replications (each with 8 birds). Chickens fed HD diet presented significantly higher body weight gain (BWG) in 15-56 days and also 0-56 days of experiment and lower feed conversion ratio (FCR) during whole time (0-56 days) of experiment (P < 0.05). Addition of CB and MCFA did not affect the differences in BWG (15-56; 0-56 days) and FCR (15-56 days) in comparison with the control diet. Significant interaction between the type of diet and additives (P < 0.05) was observed for feed intake (FI) throughout the experiment. The highest FI was found on LD diet with CB. On LD diet, all additives resulted in significantly worse nitrogen retention, however, on HD diet, it was observed only for salinomycin. The additives had no impact on fat digestibility when chickens were fed HD diet. There was significant (P < 0.05) lower-fat digestibility when LD diet with MCT, CB, and MCFA was used. The greatest value of nitrogen-corrected apparent metabolizable energy (AMEN) was found on HD diet (P < 0.05). The addition of MCT, CB, and MCFA increased the percentage share of ileum, and MCT also significantly increased the percentage share of the liver. The lactic acid bacteria and C. perfringens counts were lower on HD diet (P<0,05). The addition of MCT and CB increased the count of lactic acid bacteria in ceaca (P<0,05). Many interactions between experimental factors in this experiment showed that the efficacy of using different feed additives depends on the type of diet. The results suggest that MCFA may be the most beneficial feed additive for GLPH chicken as an alternative for antibiotic growth promoters (AGP).

Effect of storage of wet brewer’s grains with incremental levels of salt on apparent total-tract nutrient digestibility and purine derivative excretion in dairy heifers

Objectives of this study were to evaluate apparent total-tract nutrient digestibility and purine derivative (PD) excretion in dairy heifers limit-fed diets containing wet brewer’s grains (WBG) treated with salt. A 12-wk replicated 4×4 Latin square was conducted using 8 Holstein heifers of 224.5 ± 19.4 d of age, and body weight (BW) of 219.2 ± 28.1 kg (mean ± SD). Fresh WBG were treated with 0%, 0.8%, 1.6%, and 2.4% salt and stored for 4 d before being fed. Salt was added either to the WBG or separately to equalize the amount of salt in the diet. The diet contained 9% grass silage, 47% corn silage, 19% corn meal, 17.6% WBG and salt, 2% soybean meal (SBM), and 3% mineral mix. Diets were formulated to be limit-fed at 2.15% of BW, provide 14% crude protein (CP) and 2.27 Mcal metabolizable energy (ME)/kg of dry matter (DM). Heifers were adapted to diets for 14 d followed by a 7-d collection period. Dry matter intake (DMI) was recorded daily during the collection week while BW was recorded once a week. Urine and fecal samples were collected during the last 4 d of the collection period. Acid insoluble ash was used as an internal marker to determine apparent nutrient digestibility. Weight loss of WBG during storage was determined from d 1 to d 11 and initial and final yeast and mold counts were determined. Final yeast counts were similar among treatments while final mold counts tended to be lesser (P = 0.07) for the 0.8 and 1.6% salt treatments. Urinary volume was similar among treatments while allantoin (P = 0.14), and uric acid (P &lt; 0.01) and total PD excretion tended to increase (P = 0.13) quadratically. Dry matter intake was varied by treatment (linear, quadratic, and cubic effects P &lt; 0.01). Heifers fed the 0.8% treatment had the least DMI. Non-fiber carbohydrate (NFC) digestibility linearly decreased (P &lt; 0.04) as salt increased. Digestibilities of DM, and organic matter (OM), tended to decrease (P &lt; 0.10) with increasing levels of salt added to WBG. Fat digestibility was quadratic with the greatest value for the 1.6% treatment. Treating WBG with salt reduced its deterioration based on lesser mold counts for the 0.8 and 1.6% treatments. These treatments had resulted in greater fat digestibility and tended to have increased PD excretion suggesting improved microbial protein synthesis.

Supplementation of bile acids and lipase in broiler diets for better nutrient utilization and performance: Potential effects and future implications

Bile acids are used for better emulsification, digestion and absorption of dietary fat in chicken, especially in early life. Similarly, exogenous lipases have also been used for the improvement of physiological limitation of the chicken digestive system. Owing to potential of both bile acids and lipases, their use has been increased in recent years, for better emulsification of dietary fat and improvement of growth performance in broilers. In the past, pancreatic lipases were used for supplementation, but recently, microbial lipase is getting attention in poultry industry as a hydrolysis catalyst. Bile acids strengthen the defence mechanism of body against bacterial endotoxins and also play a key role in lipid regulation and sugar metabolism as signaling molecules. It has been demonstrated that bile acids and lipases may improve feed efficiency by enhancing digestive enzyme activity and ultimately leading to better fat digestion and absorption. Wide supplemental range of bile acids (0.004% to 0.25%) and lipases (0.01% to 0.1%) have been used in broiler diets for improvement of fat digestibility and their performance. Combinations of different bile acids have shown more potential to improve feed efficiency (by 7.14%) even at low (0.008%) levels as compared to any individual bile acid. Lipases at a lower level of 0.03% have exhibited more promising potential to improve fat digestibility and feed efficiency. However, contradicting results have been published in literature, which needs further investigations to elucidate various nutritional aspects of bile acids and lipase supplementation in broiler diet. This review focuses on providing insight on the mechanism of action and potential application of bile acids and lipases in broiler diets. Moreover, future implications of these additives in poultry nutrition for enhancing nutrient utilization and absorption are also discussed.

DELIVERY ROUTE OF CHAMOMILE ON THE GROWTH AND SUBSEQUENT PHYSIOLOGY OF BROILER CHICKENS UNDER E. COLI CHALLENGE

This experiment was conducted to investigate the effect of different levels (5g/kg ,10 g/kg or litter) of chamomile flower in feed and water on performance and gut health of broiler chickens under E. coli challenge. A total of 480-day-old Ross 308 broilers were randomly assigned to 12 treatments each replicated 4 times with 10 birds. Oral inoculation with E. coli was on days 8 and 9 of birds age. At day 10 and 35, the interaction (p<0.05) between the experimental factors decreased the feed intake in the challenged birds supplemented with chamomile. At day 10, unchallenged birds were heavier (p<0.05) than the challenged groups. Up to 35 days, birds on antibiotics (zinc bacitracin 360g/kg) and chamomile containing diets were heavier than other groups. The FCR improved in chamomile supplemented birds in both challenge conditions. Challenged birds had poorer FCR than unchallenged birds. The FCR improved in antibiotics and chamomile supplemented birds than those on control diets. The intestinal pH, serum lipid content decreased and serum protein profile improved by chamomile supplementation. The interaction of the experimental factors increased (p<0.0001) protein digestibility in the unchallenged birds supplemented with chamomile in their diets. Protein digestibility improved (p<0.001) in chamomile supplemented birds. The interaction of the experimental factors increased fat digestibility (p<0.0001) in the challenged birds on the lower level of dietary chamomile. Fat digestibility was higher (p<0.0001) but protein digestibility and villi were lower in the challenged birds than unchallenged groups. Longer villi were observed in the unchallenged birds on the lower level of chamomile in their drinking water and those on the highest level of dietary chamomile

Use of Milk Fat/Cellulose Ether Emulsions in Spreadable Creams and the Effect of In Vitro Digestion on Texture and Fat Digestibility

This study investigated the texture properties and fat digestibility of new spreadable chocolate creams formulated with an emulsion composed of milk fat and a cellulose ether as a fat source. The spreadability was analysed at 20 °C and compared with a commercial spreadable cream formulated with palm fat. Structural changes in the creams after the in vitro oral and gastric digestion stages were evaluated; lipid digestibility was determined by titration with NaOH during intestinal digestion. Spreadability tests showed the spreads were similar. After oral digestion, the commercial spread showed an increase in extrusion force because of flocculation induced by saliva, an effect not observed in spreads with cellulose ether. Digestibility determination showed lower values for the reformulated spreads. Therefore, milk fat-cellulose ether based emulsions offer an alternative to achieve reformulated spreadable creams, with physical properties similar to those of commercial products but providing reduced fat content and lower lipid digestibility, without compromising the quality of the final product.

Effect of Protein and Calcium Microparticle feeding with Lactobacillus acidophilus or Citric Acid on Meat Quality

This research aims to determine the effect of administering a combination feed of calcium microparticle protein with Lactobacillus acidophilus or citric acid on fat digestibility and the meat of broiler chicken. The cattle used in this research are 15 days old broiler chickens, amounted in 160 chickens. The study uses complete random design (RAL) with 5 treatments and 4 repeat treatmens (8 chickens each). The treatment applied were as follows: T0 (non-microbial feed (protein 21%)), T1 (non-microparticle feed (18% protein + Lactobacillus acidophilus 1.2 ml (108 CFU/ml)), T2 (non-microparticle feed (18% protein + Citric acid 1.2%), T3 (Microparticle feed (18%) + Lactobacillus acidophilus 1.2 ml (108 CFU/ml)), T4 (microparticle feed (18% protein + Citric acid 1.2%). The observed parameters are the digestibility of fat, fat mass of meat, the percentage of abdominal fat and the increase in body weight. The analyzed data results were variegated and continued using Duncan's multiple range test at a 5% probability. Treatment was given from the age of 15 days to 42 days. The results of the study show that the digestibility of fats that had the treatment of feeding protein and calcium microparticles with the addition of real effect Lactobacillus acidophilus is lower than the T0 and T2 but not unlike the T1 and T4 (P<0.05). The fat mass of meat and abdominal fats in the T4 treatment has a real difference (P<0.05) and at the lowest of the T0, T1 and T2 but different from T3. Higher weight increase in T3 treatment is compared to T1, T2 and T4 but not different from T0. Based on the results of the study it can be concluded that feeding microparticles of protein and calcium with Lactobacillus acidophilus 1.2 ml can decrease fat digestibility and increase weight gain but not in the fat mass of meat and abdominal fat percentage. Keywords : broiler, citric acid, Lactobacillus acidophilus, microparticle.